[Modern methods in the serological diagnosis of toxoplasmosis]. / Sovremennye metody serologicheskoi diagnostiki toksoplazmoza.

A total of 235 sera from patients suspected for toxoplasmosis were examined in the enzyme immunoassay (EIA), complement fixation, indirect immunofluorescence (IIF) and indirect hemagglutination (IHA) tests carried out in parallel. Good correlation between all tests was noted. As confirmed by the data obtained in this investigation, EIA showed the highest sensitivity. In IIF and IHA tests the maximum titers (1:512) were obtained in 2% of cases, while in EIA 17.5% of sera were positive in titers of 1:800 to 1:25,000. High sensitivity and good reproducibility of this assay suggest the necessity for its standardization and automation, which will permit its further use in laboratories at any level.

[Use of immunoenzyme analysis in the diagnosis of toxoplasmosis]. / Ispol'zovanie immunofermentnogo analiza v diagnostike toksoplazmoza.

The method for the preparation of antigen for ELISA and the technique of this assay are described. The comparative data obtained in the examination of 50 donors and 95 children suspected to have toxoplasmosis, carried out with the use of immunofluorescence and ELISA, are presented. A good correlation between the results of the both tests has been shown.

[Use of the passive hemagglutination test to diagnose toxoplasmosis]. / Primenenie reaktsii passivnoi gemagglutinatsii dlia diagnostiki toksoplazmoza.

The authors describe a method of obtaining toxoplasma erythrocytic diagnostic agent by sensitization of formalinized tannin-treated SRBC with purified toxoplasma antigen isolated by fractionation of complete toxoplasma antigen on Sephadex G-100. Comparative experiments with titration of sera of persons with suspected toxoplasmosis were conducted; the passive hemagglutination test with the antigen obtained proved to be highly sensitive in comparison with immunofluorescence and complement fixation tests.