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1.
Front Psychol ; 14: 1071985, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37292512

RESUMO

Personality is considered as the internal factor that defines a person's behavior. Therefore, providing adaptive features and personalized support in online learning by considering learners' personalities can improve their learning experiences and outcomes. In this context, several research studies have investigated the impact of personality differences in online learning. However, little is known about how personality differences affect learners' behavior while learning. To fill this gap, this study applies a lag sequential analysis (LSA) approach to understand learners' navigational behavior patterns in an online three-months course of 65 learners based on their personalities. In this context, the five factor model (FFM) model was used to identify learners' personalities. The findings revealed that learners with different personalities use different strategies to learn and navigate within the course. For instance, learners high in extraversion tend to be extrinsically motivated. They therefore significantly navigated between viewing the course module and their personal achievements. The findings of this study can contribute to the adaptive learning field by providing insights about which personalization features can help learners with different personalities. The findings can also contribute to the field of automatic modeling of personality by providing information about differences in navigational behavior based on learners' personalities.

2.
J Muscle Res Cell Motil ; 42(2): 381-397, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33710525

RESUMO

Hypertrophic cardiomyopathy (HCM) often leads to heart failure. Mutations in sarcomeric proteins are most frequently the cause of HCM but in many patients the gene defect is not known. Here we report on a young man who was diagnosed with HCM shortly after birth. Whole exome sequencing revealed a mutation in the FLNC gene (c.7289C > T; p.Ala2430Val) that was previously shown to cause aggregation of the mutant protein in transfected cells. Myocardial tissue from patients with this mutation has not been analyzed before and thus, the underlying etiology is not well understood. Myocardial tissue of our patient obtained during myectomy at the age of 23 years was analyzed in detail by histochemistry, immunofluorescence staining, electron microscopy and western blot analysis. Cardiac histology showed a pathology typical for myofibrillar myopathy with myofibril disarray and abnormal protein aggregates containing BAG3, desmin, HSPB5 and filamin C. Analysis of sarcomeric and intercalated disc proteins showed focally reduced expression of the gap junction protein connexin43 and Xin-positive sarcomeric lesions in the cardiomyocytes of our patient. In addition, autophagy pathways were altered with upregulation of LC3-II, WIPI1 and HSPB5, 6, 7 and 8. We conclude that the p.Ala2430Val mutation in FLNC most probably is associated with HCM characterized by abnormal intercalated discs, disarray of myofibrils and aggregates containing Z-disc proteins similar to myofibrillar myopathy, which supports the pathological effect of the mutation.


Assuntos
Cardiomiopatia Hipertrófica , Filaminas , Miopatias Congênitas Estruturais , Proteínas Adaptadoras de Transdução de Sinal , Adulto , Proteínas Reguladoras de Apoptose , Cardiomiopatia Hipertrófica/genética , Filaminas/genética , Humanos , Masculino , Mutação , Miócitos Cardíacos , Adulto Jovem
3.
Proc Natl Acad Sci U S A ; 115(49): 12531-12536, 2018 12 04.
Artigo em Inglês | MEDLINE | ID: mdl-30442670

RESUMO

During early postnatal development, sensory regions of the brain undergo periods of heightened plasticity which sculpt neural networks and lay the foundation for adult sensory perception. Such critical periods were also postulated for learning and memory but remain elusive and poorly understood. Here, we present evidence that the activity-regulated and memory-linked gene Arc/Arg3.1 is transiently up-regulated in the hippocampus during the first postnatal month. Conditional removal of Arc/Arg3.1 during this period permanently alters hippocampal oscillations and diminishes spatial learning capacity throughout adulthood. In contrast, post developmental removal of Arc/Arg3.1 leaves learning and network activity patterns intact. Long-term memory storage continues to rely on Arc/Arg3.1 expression throughout life. These results demonstrate that Arc/Arg3.1 mediates a critical period for spatial learning, during which Arc/Arg3.1 fosters maturation of hippocampal network activity necessary for future learning and memory storage.


Assuntos
Proteínas do Citoesqueleto/metabolismo , Hipocampo/fisiologia , Memória de Longo Prazo/fisiologia , Proteínas do Tecido Nervoso/metabolismo , Aprendizagem Espacial/fisiologia , Animais , Comportamento Animal , Inibidor de Quinase Dependente de Ciclina p21/genética , Inibidor de Quinase Dependente de Ciclina p21/metabolismo , Proteínas do Citoesqueleto/genética , Deleção de Genes , Regulação da Expressão Gênica/fisiologia , Camundongos , Proteínas do Tecido Nervoso/genética , Plasticidade Neuronal , Neurônios/fisiologia
4.
Cancer Res ; 76(19): 5845-5856, 2016 10 01.
Artigo em Inglês | MEDLINE | ID: mdl-27488520

RESUMO

Hypoxia is a common feature of solid tumors, which controls multiple aspects of cancer progression. One important function of hypoxia and the hypoxia-inducible factors (HIF) is the maintenance of cancer stem-like cells (CSC), a population of tumor cells that possess stem cell-like properties and drives tumor growth. Among the changes promoted by hypoxia is a metabolic shift resulting in acidification of the tumor microenvironment. Here, we show that glioma hypoxia and acidosis functionally cooperate in inducing HIF transcription factors and CSC maintenance. We found that these effects did not involve the classical PHD/VHL pathway for HIF upregulation, but instead involved the stress-induced chaperone protein HSP90. Genetic or pharmacologic inactivation of HSP90 inhibited the increase in HIF levels and abolished the self-renewal and tumorigenic properties of CSCs induced by acidosis. In clinical specimens of glioma, HSP90 was upregulated in the hypoxic niche and was correlated with a CSC phenotype. Our findings highlight the role of tumor acidification within the hypoxic niche in the regulation of HIF and CSC function through HSP90, with implications for therapeutic strategies to target CSC in gliomas and other hypoxic tumors. Cancer Res; 76(19); 5845-56. ©2016 AACR.


Assuntos
Acidose/metabolismo , Fatores de Transcrição Hélice-Alça-Hélice Básicos/fisiologia , Neoplasias Encefálicas/patologia , Glioma/patologia , Proteínas de Choque Térmico HSP90/fisiologia , Subunidade alfa do Fator 1 Induzível por Hipóxia/fisiologia , Células-Tronco Neoplásicas/fisiologia , Prolil Hidroxilases/fisiologia , Proteína Supressora de Tumor Von Hippel-Lindau/fisiologia , Animais , Neoplasias Encefálicas/metabolismo , Linhagem Celular Tumoral , Feminino , Glioma/metabolismo , Humanos , Concentração de Íons de Hidrogênio , Camundongos
5.
Mol Cell Proteomics ; 13(8): 1925-36, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24763107

RESUMO

To quantify cell cycle-dependent fluctuations on a proteome-wide scale, we performed integrative analysis of the proteome and phosphoproteome during the four major phases of the cell cycle in Schizosaccharomyces pombe. In highly synchronized cells, we identified 3753 proteins and 3682 phosphorylation events and relatively quantified 65% of the data across all phases. Quantitative changes during the cell cycle were infrequent and weak in the proteome but prominent in the phosphoproteome. Protein phosphorylation peaked in mitosis, where the median phosphorylation site occupancy was 44%, about 2-fold higher than in other phases. We measured copy numbers of 3178 proteins, which together with phosphorylation site stoichiometry enabled us to estimate the absolute amount of protein-bound phosphate, as well as its change across the cell cycle. Our results indicate that 23% of the average intracellular ATP is utilized by protein kinases to phosphorylate their substrates to drive regulatory processes during cell division. Accordingly, we observe that phosphate transporters and phosphate-metabolizing enzymes are phosphorylated and therefore likely to be regulated in mitosis.


Assuntos
Proteômica/métodos , Proteínas de Schizosaccharomyces pombe/análise , Schizosaccharomyces/citologia , Schizosaccharomyces/fisiologia , Trifosfato de Adenosina/metabolismo , Técnicas de Cultura de Células , Ciclo Celular , Regulação Fúngica da Expressão Gênica , Espectrometria de Massas/métodos , Fosforilação , Proteoma/análise
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