An Investigation of the Effect of Irradiation on the Biomechanical Properties of Fibular Grafts.

PURPOSE: Irradiation of fibular grafts for jaw reconstruction following mandibulectomy can be associated with increased graft failure and implant instability. The objective of this study is to investigate the effect of isolated irradiation on the biomechanical properties of ex-vivo porcine fibula grafts. It was hypothesized that the isolated irradiation (without biological response) will not significantly influence the biomechanical properties of the fibular grafts. METHODS: Forty porcine fibular grafts (n = 20 irradiated and n = 20 nonirradiated) were obtained. The irradiated group was subjected to a single dose of 60 Gy of irradiation with cesium 137. Both groups underwent 3-point bending tests with cyclic loading for 1500 cycles (50 to 500 N at 2 Hz) followed by push to failure. Rate of damage (mm/s), stiffness (N/mm), modulus of elasticity (MPa), maximum load (N), maximum displacement (mm), and maximum stress (MPa) were compared between the 2 groups using independent t tests (P < .05). RESULTS: No significant differences were found between the irradiated and nonirradiated groups for any of the biomechanical parameters (all P values > 0.05). CONCLUSIONS: The results of this study support our hypothesis that even high levels of isolated irradiation do not significantly affect the biomechanical properties of fibular bone grafts. This work allows us to exclude acute structural changes in the bone due to irradiation as a possible factor leading to bone/implant instability following jaw reconstruction with fibular grafts. Further studies need to be conducted to better understand the range of factors that may lead to implant instability, including the biological response after radiation therapy.

Biomechanical Strength of All-Inside ACL Reconstruction Grafts Using Side-to-Side and Backup Fixation.

BACKGROUND: The all-inside anterior cruciate ligament reconstruction (ACLR) procedure uses a single hamstring tendon folded twice and secured to itself to form a 4-stranded graft. There are several possible configurations for preparing the graft. PURPOSE: To investigate the biomechanical properties of a new graft preparation technique in comparison with 2 commonly used configurations. STUDY DESIGN: Controlled laboratory study. METHODS: Five porcine flexor tendons were prepared into the test graft configuration: side-to-side fixation with a backup fixation at the button loop (graft M). The test configuration was compared with the results of a previous study that included grafts with simple interrupted sutures (graft A; n = 5) and end-to-end fixation (graft C; n = 5). All grafts were subjected to the same mechanical testing protocol to determine the mean failure load, stiffness, rate of elongation, and total elongation during both cyclic loading and pull to failure. Differences between groups were evaluated. RESULTS: Graft A had a significantly lower failure load (637 ± 99 N) compared with graft M (883 ± 66 N; P = .002) and graft C (846 ± 26 N; P = .002). Graft A also had significantly lower stiffness (166 ± 12 N/mm) compared with graft M (215 ± 8 N/mm; P < .001) and graft C (212 ± 11 N/mm; P < .001). Graft C had a significantly lower elongation during cyclic loading (3.42 ± 0.24 mm) compared with graft M (4.37 ± 0.74 mm; P = .026) and graft A (4.90 ± 0.88 mm; P = .006). The unsecured fixation was the weakest graft, with the lowest failure load and stiffness. The new side-to-side configuration and end-to-end configuration were equally strong. CONCLUSION: The new side-to-side configuration was not biomechanically superior to the end-to-end configuration; however, they were both stronger than unsecured fixation. CLINICAL RELEVANCE: As the all-inside ACLR is gaining popularity, this study provides surgeons with a new method of preparing grafts and evaluates the method with respect to currently used configurations.