[Leaking index--a new urodynamic parameter in the diagnosis of female stress urinary incontinence]. / Wskaznik wyciekania--nowy parametr urodynamiczny w ocenie wysilkowego nietrzymania moczu u kobiet.

UNLABELLED: Stress urinary incontinence is the most prevalent kind of urinary incontinence in women. Treatment efficiency depends on the type of stress urinary incontinence. PURPOSE OF THE STUDY: The purpose of the study was to assess the differences of values of leaking indexes in women with different types of stress urinary incontinence. MATERIAL AND METHODS: 64 patients were hospitalized at Obstetrics and Gynecology Department of Silesian Medical University between 2005 and 2007. All patients included in the study were diagnosed with stress urinary incontinence (SUI) based on medical history clinical evaluation, pad test and urodynamic evaluation. The study population was divided into three groups according to VLPP and MUCP. Leaking indexes were calculated as a quotient of the area under the curve of leakage and the area under the curve of bladder pressure during the Valsalva manoeuvre. RESULTS: The values of leaking indexes and relative leaking indexes in different types of SUI were compared. Statistically significant differences between relative leaking indexes for groups of women divided according to MUCP and VLPP criterion were found. CONCLUSIONS: Judging from statistically significant differences in relative leaking indexes between groups of women with different types of SUI, relative leaking indexes might be of use in differential diagnosis of types of SUI.

[Evaluation of wild-type estrogen receptor beta and its isoforms: ER-beta/delta 5/6 i ER-beta/delta 6 in endometrial adenocarcinoma]. / Ocena profilu ekspresji receptora estrogenowego beta i jego izoform ER-beta/delta 5/6 i ER-beta/delta 6 w gruczolakoraku endometrium.

Estrogen target genes play essential role among pathogenetic and prognostic factors of endometrial adenocarcinoma; and among them estrogen receptor genes. Although prevailing estrogen receptor type is ER-alpha in endometrium, transduction of signal carried by estrogens can be also mediate by ER-beta, which biological meaning relies on modulation of ER-alpha transcriptional activity. There is hypothesized that ER-beta can keep under control mitogenic activity mediating by ER-alpha. The aim of the study was qualitative and quantitative analysis of wtER-beta and ER-beta/delta 5-6, ER-beta/delta 6 isoforms in endometrial adenocarcinoma. Material used in the study included specimens obtained from 27 female: group I--normal endometrium (n = 12), group II--endometrial adenocarcinoma (n = 15). RNA was extracted from the analyzed material using phenol-chloroform method by Total RNA Prep Plus. Qualitative analysis was performed basing on RT-PCR reaction and polyacrylamide gel electrophoreses. For all RNA extracts of the samples examined RT-PCR was performed with a sequence detector ABIPRISMTM7700--Perkin-Elmer Applied Biosystems (RT: 600 C-30 min; PCR: 950 C-5 min, 40 cycles: 950 C-30s, 600 C-1 min, and 720 C-10 min) in order to determine the number of RNA copies (which corresponds to the number of cDNA copies) for wtER-beta and ER-beta/delta 5-6 and ER-beta/delta 6. Specificity of QRT-PCR reaction was determined by delimitation of melting temperature of all examined amplimers (ABI PRISM7000, Qu-antiTect SYBR Green RT-PCR Kit) and by sequence analysis using ABI PRISM377 DNA Sequencer. Wild-type of estrogen receptor beta and ER-beta/delta 5-6, ER-beta/delta 6 isoforms--coming into alternative splicing of mRNA, presented both, in proliferative endometrium and endometrial adenocarcinoma. Copy number of wtER-beta mRNA was significantly (p < 0.05, Mann-Whitney U test) lower in comparison with proliferative endometrium and correlated with decrease of ER-beta/delta 6 mRNA copy number (r = 0.85; p < 0.05). Preliminary results confirming decrease of wtER-beta, ER-beta/delta 5/6 and ER-beta/delta 6 mRNA copy number in endometrial adenocarcinoma can show their relationship with high risk of carcinogenesis.

[Expression patterns in isoforms of vascular endothelial growth-factors in tissue samples of vulval cancer T1 N2M0 stage]. / Wzory ekspresji izoform naczyniowo-sródblonkowego czynnika wzrostu (VEGF) w raku sromu T1N2M0.

OBJECTIVES: The aim of the study was estimating the expression of isoforms of mRNA vascular endothelial growth factor: VEGF121, VEGF145, VEGF165, VEGF183, VEGF189, VEGF206, in tissue samples of vulvar cancer, normal and inguinal lymph nodes metastases. MATERIALS AND METHODS: The material for this investigation of expression of mRNA selected genes was 4 tissue specimens from women with vulvar cancer T1N2M0. The method used for quantitative determination of the numbers of mRNA copies of selected genes was the QRT-PCR and QPCR technique, with the use of an ABI PRISM 7700 (TaqMan) sequence detector. The nucleotide sequence for starters and probes for quantitative RT-PCR was designed with the use of the Primer Express Version 1.0 ABI PRISM software. RESULTS: Expression of all mRNA isoforms VEGF was found in the samples. The number of mRNA copies obtained in 1 microgram total mRNA extract of the genes examined in tissue sections with various pathomorphology were clearly differentiated. CONCLUSIONS: Expression profile of aforementioned genes indicated a definitive relationship between vascular endothelial growth factor isoform and vulvar cancer. These findings provide evidence that the expression pattern of mRNA VEGF isoform should find broad applications in clinical and research settings.