Dermatoglyphic Pattern Assessment in Smokeless Tobacco Users in the North Gujarat Population: A Comparative Case-Control Study.

Introduction: Dermatoglyphic patterns identify genetic conditions as they develop early in pregnancy and remain constant throughout life. Multiple studies suggest that there is a genetic link to smokeless tobacco use. So, this study has been conducted to assess dermatoglyphic patterns among smokeless tobacco users in the North Gujarat population with nonconsumers. Materials and Methods: A total of 500 cases were investigated for "ATD" angle and patterns, of which 250 subjects of smokeless tobacco are assessed for statistical significance via comparing them to 250 subjects of nonconsumers. Student's t-test was employed for evaluating quantitative factors (also known as the "ATD" angle), and the Chi-square test was used to analyze qualitative variables (also known as fingerprint patterns). A P value of 0.05 or less was considered significant. Results: In this study, the angle "ATD" was found to be reduced in both the palms of males (P 0.001) and females (P 0.001) when compared to controls, which was statistically highly significant. In our study, we discovered that the number of whorls and arches is lower in male cases as compared to male controls, which is highly significant (P = 0.020 and P = 0.010). Male cases had a greater number of loops than male controls, which was statistically significantly greater (P 0.001). Conclusion: In this latest study, an association between smokeless tobacco users and palmar dermatoglyphics was identified. Though dermatoglyphics solely is unable to identify individuals who abuse alcohol and/or cigarette packs, the results of this research could serve to further develop diagnostic guidelines.

Effect of Dentin Bonding Agent on Intrapulpal Temperature during Fabrication of Provisional Restorations by a Direct Method: An In Vitro Study.

AIM: To determine the effect of three different provisional restorative materials (PRMs) and application of dentin bonding agent (DBA) on intrapulpal temperature rise during fabrication of provisional crowns using a direct method. MATERIALS AND METHODS: Three PRMs-polymethyl methacrylate resin (PMMA), bis-acrylic resin, and dimethyl methacrylate resin (DMMA)-were used in the study. Dentin bonding agent (Single Bond 2, 3M ESPE, Germany) was applied in a single layer or double layer as an insulating material. A total of 45 molars were prepared for complete coverage restoration with chamfer finish line and 1.5 mm axial and 2 mm occlusal reduction. After application of a single or double layer of DBA, provisional materials were polymerized according to the manufacturer's instructions. The temperature increase inside the pulp chamber was measured with a T-type thermocouple wire. The readings were averaged for each group to determine the mean value of temperature rise. RESULTS: Statistical analysis was performed with analysis of variance (ANOVA) followed by post hoc Tukey test (a = 0.05). The temperature rise varied according to the provisional restoration material used (PMMA > DMMA > bis-acrylic resin) (p < 0.001) and the single or double layer of DBA (p < 0.001). Polymethyl methacrylate resin produced a higher intrapulpal temperature. CONCLUSION: Application of a double layer of DBA resulted in a significantly lower intrapulpal temperature.

Assessment and correlation between functional and histological staging of oral submucous fibrosis: A clinicohistopathologic study.

BACKGROUND AND OBJECTIVE: Oral submucous fibrosis (OSMF) is a precancerous condition. It is widespread in the Asian subcontinent, with India bearing most of the burden. It is characterized by mucosal rigidity of varying intensity due to the fibroelastic changes of the juxta epithelial layer, resulting in a progressive inability to open the mouth. Early recognition with accurate staging of the disease and appropriate treatment planning is of utmost importance to prevent the malignant transformation and to improve the quality of life of the patient. In the present study, an attempt is made to clinically evaluate the condition and correlate it with the histopathological findings according to standard criteria. MATERIALS AND METHODS: A hospital-based study was conducted on sixty OSMF patients. Detailed history was recorded, and functional staging was given depending on mouth opening. Punch biopsy was performed, and histological stages were given based on standard criteria. The data so received were mathematically evaluated to determine whether any correlation exists between the stages using Chi-square test. RESULTS: The sixty patients were in the age range of 16-50 years. Male-to-female ratio was that of 97:3. The statistical analysis using Chi-square test showed statistically significant association (P < 0.001) between the functional and histologic stages. CONCLUSION: There is a definite correlation between functional and histological stages of OSMF which suggests that clinically advanced OSMF has extensive fibrosis histologically.

Amelogenin Gene - The Pioneer in Gender Determination from Forensic Dental Samples.

INTRODUCTION: In the event of any mass fatality incident, DNA analysis plays a vital role in disaster victim identification. Teeth are one of the most resistant structures in the human body that resist decomposition hence making them prime choice for extracting DNA for identification of individuals. Polymerase Chain Reaction (PCR) analysis that target regions of Amelogenin gene have become the method of choice for sex determination of biological samples. AIM: Determining the sex of a given DNA sample from either dental pulp or dentin of tooth and help in identification of missing persons and disaster victims. MATERIALS AND METHODS: In our study 50 teeth samples were studied and they were subjected to various environmental conditions along with freshly extracted teeth taken as control for duration of one month and three months. Pulpal tissue was retrieved from the teeth specimens by access opening of root canals and for incinerated samples, the specimens were crushed. From the DNA that was extracted from the dental pulp sample Amelogenin gene locus was used for sex determination by amplifying a segment of X-Y homologous gene locus through PCR analysis. RESULTS: ANOVA test and t-test proved to be statistically significant and 100% retrieval rate was observed in samples. CONCLUSION: Pulpal tissue along with degenerating odontoblastic processes yield sufficient amount of DNA for gender determination when subjected to various forensic conditions with maximum accuracy.

In vitro Evaluation of the Accuracy of Seating Cast Metal Fixed Partial Denture on the Abutment Teeth with Varying Degree of Convergence Angle.

BACKGROUND: The prime goal of a diligent prosthodontist is to obtain adequate marginal fit while restoring lost tooth structure. The marginal fit of the restoration, in turn depends upon the geometrical morphology of the tooth preparation. OBJECTIVE: To determine the effect of varying degree of convergence angle on the marginal seating of the single crown, three-unit fixed partial denture and multiple-unit fixed partial denture with pier abutment. MATERIALS AND METHODS: Three dies, of same convergence angle, were placed in an arch form on a base. In this way, four arch forms were prepared for four different convergence angles i.e. 0°, 6°, 12°, and 20°. Five castings each were made for single crown, 3-unit fixed partial denture and multiple-unit fixed partial denture (FPD) with pier abutment for each convergence angle. The castings were seated on their respective dies and vertical marginal discrepancy was measured at four points for each casting with the help of an optical microscope. RESULTS: The results showed that 200 convergence angle showed better marginal seating of the single crown, 3-unit FPD as well as for the multiple unit FPD. CONCLUSION: There was a possibility that the retention and resistance may be compromised with 200 taper. Hence 120 taper is suggested for crowns and fixed partial denture retainers as the marginal discrepancy is reasonable and retention and resistance is optimum. There was a high statistical significant difference in the values obtained for the different convergence angles.

Prosthetic Rehabilitation of Ocular Defect resulting from Pediatric Retinoblastoma.

Ocular defects result from tumor, congenital anomaly and external injury not only lead to serious impairment of function and esthetics but also make the patient psychologically disabled. Prosthetic rehabilitation attempts to restore these disfgurements may improve esthetic, level of function, general psychologic improvement and quality of life. This clinical report details an attempt to rehabilitate a pediatric patient who has undergone orbital enucleation resulting from retinoblastoma with the aid of custom ocular prosthesis using commercially available prefabricated eye shell. How to cite this article: Janya S, Gubrellay P, Purwar A, Khanna S. Prosthetic Rehabilitation of Ocular Defect resulting from Pediatric Retinoblastoma. Int J Clin Pediatr Dent 2014; 7(3):209-212.