Integrated biomarker response to assess the effects of pesticide residues on Nile Tilapia in aquatic ecosystems contaminated by cotton-field effluents.

An in-situ study combined with an integrated biomarker response was used to evaluate the impact of agricultural effluents in the physiological responses of Nile tilapia reared in cages and enclosures of water reservoirs in North Benin. Fish were distributed in fish farming systems at two sites: Songhai located outside the cotton basin and Batran located in the most productive commune. They were sampled for blood and organs before (BST), during (DST) and after (AST) pesticide treatment. Pesticide residues were analysed in water, sediments and fish muscles. Several biomarkers were investigated related to the immune (peroxidase, lysozyme and complement activities, superoxide anion production) and reproductive (sex steroids and vitellogenin levels) responses as well as neurotoxicity (cholinesterase activity) and tissue alterations. Biomarkers were assessed and analysed via the integrated biomarker response (IBR). The results showed that Batran water reservoir was a more harmful ecosystem for fish than Songhai one, especially by depressing some immune and reproductive functions in relation to a higher-level of pesticide contamination. They also demonstrated that the contact of fish to sediments in enclosures aggravated the pesticide burden on fish. Therefore, using males as bioindicators would improve the sensitivity of the used biomarkers since males seemed more affected than females especially due to pesticide estrogenic induction impacting their reproductive system.

Chronic effects of a binary insecticide Acer 35 EC on Nile tilapia Oreochromis niloticus through a multi-biomarker approach.

Acer 35 EC is a widely used insecticide (a binary mixture of lambda-cyhalothrin and acetamiprid) in pest control in many West African countries, particularly in the cotton culture in north Benin. The aim of this study was to investigate the chronic effects of Acer 35 EC on Nile tilapia Oreochromis niloticus juveniles using a multi-biomarker approach under laboratory conditions. For this purpose, fish were exposed to sublethal concentrations of Acer 35 EC (0, 1 and 10% of LC50- 96 h value). After 28 and 56 days of exposure, several biomarkers were measured in males and females including enzymatic activities related to detoxification and oxidative stress, neurotoxicity and immune responses, sex steroid hormones (testosterone, 17ß-estradiol and 11-keto-testosterone) and histological alterations of liver, kidney and gonads. An Integrated Biomarker Response (IBR) was then calculated. The results showed a reduction of cholinesterase activity in muscles, and intercellular superoxide anion production in both sexes. Female steroidogenesis and gametogenesis were affected, especially testosterone levels and oocyte growth. More alterations were observed in liver after exposure to Acer 35 EC. In both sexes, IBR values were higher after 56 days than after 28 days of exposure. In conclusion, based on a large set of biomarkers and IBR values, the chronic exposure to low doses of insecticide Acer 35 EC seems to impair different physiological functions in Nile tilapia juveniles on a time-dependent manner, with a stronger impact on females than on males.