Assuntos
Carcinoma Pulmonar de Células não Pequenas/patologia , Neoplasias Pulmonares/patologia , Carcinomatose Meníngea/secundário , Neoplasias do Nervo Óptico/secundário , Adenocarcinoma de Pulmão/patologia , Evolução Fatal , Angiofluoresceinografia , Fundo de Olho , Humanos , Neoplasias Hepáticas/secundário , Masculino , Carcinomatose Meníngea/diagnóstico , Carcinomatose Meníngea/patologia , Pessoa de Meia-Idade , Invasividade Neoplásica , Nervo Óptico/patologia , Neoplasias do Nervo Óptico/diagnósticoRESUMO
Asthma and obesity are both common conditions, which lead to a substantial public health burden. The obese-asthma phenotype is characterized by poor asthma control, impaired lung function and decreased efficacy of inhaled treatment. However, this phenotype is highly heterogeneous and involves numerous mechanisms, including systemic inflammation and adipokines. A role for microbiota modifications and genetics has been suggested. Obese-asthma patient management currently consists in weight loss and usual anti-asthmatic treatment. New therapeutic options are being evaluated.
Assuntos
Asma/complicações , Asma/terapia , Obesidade/complicações , Obesidade/terapia , Adipocinas/fisiologia , Antiasmáticos/uso terapêutico , Asma/epidemiologia , Asma/metabolismo , Humanos , Inflamação/complicações , Inflamação/metabolismo , Inflamação/fisiopatologia , Obesidade/epidemiologia , Obesidade/metabolismo , Fenótipo , Transdução de Sinais/fisiologia , Programas de Redução de Peso/métodosRESUMO
Pyruvate kinase isozymic changes were studied in the adult hepatocyte cultures, by electrophoretic, kinetic and immunological methods. We were able to maintain parenchymal cells from normal adult rat liver in non-proliferating monolayer cultures up to 10 days. Hepatocytes appeared to contain a dominant PK I type up to 4-5 days of culture. After day 5, PK III type was regularly present with PK I and after 7 days PK III type was always the only isozyme detected in culture. It must be pointed out that, by the Ouchterlony method and sometimes by electrophoresis, concentrated extracts from freshly isolated hepatocytes or starting hepatocyte cultures did also contain Pyruvate kinase PK III type. These results suggest that Pyruvate kinase III is present but partly repressed in the adult parenchymal cells and becomes derepressed in culture.