RESUMO
Low-cost microbial remediation strategies serve as a viable and potent weapon for curbing the arsenic menace. In the present study, two arsenic-resistant bacteria were isolated from the contaminated lentil rhizosphere in Gangetic plain of eastern India. LAR-21 (Burkholderia cepacia, MW356875) and LAR-25 (Burkholderia cenocepacia, MW356894) could remove 87.6% and 85.9% of arsenite (10 mM) from the liquid culture medium in laboratory condition. They were highly resistant to arsenate and arsenite and also had a high arsenite oxidase activity. LAR-21 showed the highest level of minimum inhibitory concentration value of 390 mM for arsenate and 31 mM for arsenite. The same strain was found to show highest arsenite oxidase activity, i.e., 5.2 nM min-1 mg-1of protein. These two strains further possess potential plant growth-promoting characteristics like indole acetic acid production (5-15 mM IAA mL-1), 1-aminocyclopropane-1-carboxylate deaminase (8-21 nM α-keto butyrate mg protein-1 h-1), nitrogenase activity (3-8.99 nM ethylene mg cell protein-1 h-1), siderophore production (17-22.1 µM deferoxamine mesylate mL-1), phosphate solubilization (261-453 µg mL-1) under arsenic stress condition. The plant growth promotion of the strains was further validated by pot study of lentil by assessing their agronomic and growth-related traits, and potential to recover from arsenic stress (17.2-21.2% arsenic reduction in root and shoot, 16-19.2% in leaf and pod, and 15-23% reduction in seeds). The LAR-21 strain, thus, emerged as the most suited candidate for bioremediation and plant (lentil) growth promotion in arsenic polluted environment.
RESUMO
The problem of arsenic (As) pollution being severe warrants opting for low-cost microbial remediation strategies. The present study of identifying suitable bacterial strains led to the isolation of eleven As-tolerant strains from the As-contaminated rhizosphere soils of West Bengal, India. They were found to oxidize/reduce 55-31.6% of 5 mM As(III) and 73-37.6% of 5 mM As(V) within 12 h. The four isolates (BcAl-1, JN 73, LAR-2, and AR-30) had a high level of As(III) oxidase activity along with a higher level of As(V) and As(III) resistance. The agar diffusion assay of the isolates further confirmed their ability to endure As stress. The presence of aoxB gene was observed in these four As(III) oxidizing isolates. Evaluation of plant growth-promoting characteristics revealed that BcAl-1 (Burkholderia cepacia), JN 73 (Burkholderia metallica), AR-30 (Burkholderia cenocepacia), and LAR-2 (Burkholderia sp.) had significant plant growth-promoting characteristics (PGP), including the ability to solubilize phosphate, siderophore production, indole acetic acid-like molecules production, ACC deaminase production, and nodule formation under As stressed condition. BcAl-1 and JN 73 emerged as the most promising traits in As removal as well as plant growth promotion.
