Identity Test of Single NV

Atomiclike defects in solids are not considered to be identical owing to the imperfections of host lattice. Here, we found that even under ambient conditions, negatively charged nitrogen-vacancy (NV^{-}) centers in diamond could still manifest identical at Hz-precision level, corresponding to a 10^{-7}-level relative precision, while the lattice strain can destroy the identity by tens of Hz. All parameters involved in the NV^{-}-^{14}N Hamiltonian are determined by formulating six nuclear frequencies at 10-mHz-level precision and measuring them at Hz-level precision. The most precisely measured parameter, the ^{14}N quadrupole coupling P, is given by -494 575 4.9(8) Hz, whose precision is improved by nearly 4 orders of magnitude compared with previous measurements. We offer an approach for performing precision measurements in solids and deepening our understandings of NV centers as well as other solid-state defects. Besides, these high-precision results imply a potential application of a robust and integrated atomiclike clock based on ensemble NV centers.

A flexible nitrogen-vacancy center probe for scanning magnetometry.

The key component of the scanning magnetometry based on nitrogen-vacancy centers is the diamond probe. Here, we designed and fabricated a new type of probe with an array of pillars on a (100 µm)2 × 50 µm diamond chip. The probe features high yield, convertibility to be a single pillar, and expedient reusability. Our fabrication is dramatically simplified by using ultraviolet laser cutting to shape the chip from a diamond substrate instead of additional lithography and time-consuming reactive ion etching. As an example, we demonstrate the imaging of a single magnetic skyrmion with nanoscale resolution. In the future, this flexible probe will be particularly well-suited for commercial applications.

Experimental Constraint on an Exotic Parity-Odd Spin- and Velocity-Dependent Interaction with a Single Electron Spin Quantum Sensor.

Improved laboratory limits on the exotic spin- and velocity-dependent interaction at the micrometer scale are established with a single electron spin quantum sensor. The single electron spin of a near-surface nitrogen-vacancy center in diamond is used as the quantum sensor, and a fused-silica half-sphere lens is taken as the source of the moving nucleons. The exotic interaction between the polarized electron and the moving nucleon source is explored by measuring the possible magnetic field sensed by the electron spin quantum sensor. Our experiment sets improved constraints on the exotic spin- and velocity-dependent interaction within the force range from 1.4 to 330 µm. The upper limit of the coupling g_{A}^{e}g_{V}^{N} at 200 µm is |g_{A}^{e}g_{V}^{N}|≤5.3×10^{-19}, significantly improving the current laboratory limit by more than 4 orders of magnitude.

Spin-Torque Memristors Based on Perpendicular Magnetic Tunnel Junctions for Neuromorphic Computing.

Spin-torque memristors are proposed in 2009, and can provide fast, low-power, and infinite memristive behavior for neuromorphic computing and large-density non-volatile memory. However, the strict requirements of combining high magnetoresistance, stable domain wall pinning and current-induced switching in a single device pose difficulties in physical implementation. Here, a nanoscale spin-torque memristor based on a perpendicular-anisotropy magnetic tunnel junction with a CoFeB/W/CoFeB composite free layer structure is experimentally demonstrated. Its tunneling magnetoresistance is higher than 200%, and memristive behavior can be realized by spin-transfer torque switching. Memristive states are retained by strong domain wall pinning effects in the free layer. Experiments and simulations suggest that nanoscale vertical chiral spin textures can form around clusters of W atoms under the combined effect of opposite Dzyaloshinskii-Moriya interactions and the Ruderman-Kittel-Kasuya-Yosida interaction between the two CoFeB free layers. Energy fluctuation caused by these textures may be the main reason for the strong pinning effect. With the experimentally demonstrated memristive behavior and spike-timing-dependent plasticity, a spiking neural network to perform handwritten pattern recognition in an unsupervised manner is simulated. Due to advantages such as long endurance and high speed, the spin-torque memristors are competitive in the future applications for neuromorphic computing.

Transcriptome analysis reveals potential immune function-related regulatory genes/pathways of female Lubo goat submandibular glands at different developmental stages.

BACKGROUND: The submandibular glands, as major salivary glands, participate in rumen digestion in goats. Sialic acid, lysozyme, immunoglobulin A (IgA), lactoferrin and other biologically active substances secreted in the submandibular glands were reported in succession, which suggests that the submandibular gland may have immune functions in addition to participating in digestion. The aim of this study was to map the expression profile of differentially expressed genes (DEGs) at three different stages by transcriptome sequencing, screen immune-related genes and pathways by bioinformatics methods, and predict the immune function of submandibular glands at different developmental stages. METHODS: Nine submandibular gland tissue samples were collected from groups of 1-month-old kids, 12-month-old adolescent goats and 24-month-old adult goats (3 samples from each group), and high-throughput transcriptome sequencing was conducted on these samples. The DEGs among the three stages were screened and analysed. Key genes and signalling pathways were selected via protein-protein interaction (PPI) network analysis. RESULTS: The results revealed 2,706, 2,525 and 52 DEGs between 1-month-old and 12-month-old goats, between 1-month-old and 24-month-old goats, and between 12-month-old and 24-month-old goats, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses indicated that most of the DEGs were enriched in immune- related GO terms and pathways. Based on functional enrichment analysis and network analysis, 10 genes (PTPRC, CD28, SELL, LCP2, MYC, LCK, ZAP70, ITGB2, SYK and CCR7), two signalling pathways (the T cell receptor signalling pathway and the NF-κß signalling pathway) and eight GO terms (T cell receptor signalling pathway, neutrophil mediated immunity, B cell mediated immunity, regulation of alpha-beta T cell activation, positive regulation of T cell proliferation, regulation of leukocyte differentiation, positive regulation of antigen receptor-mediated signalling pathway, positive regulation of lymphocyte proliferation) that may play key roles in the immune functions of the goat submandibular glands at different developmental stages were identified. Moreover, we found that eight antibacterial peptide-encoding genes were downregulated in the tuberculosis and salivary secretion pathways, while all immunoglobulins were upregulated in 10 immune system pathways. These findings indicate that the submandibular glands may be important immunological organs during the growth process of goats and that the immune function of these glands gradually weakens with age up to 12 months but remains relatively stable after 12 months of age. Overall, this study will improve our understanding of transcriptional regulation related to goat submandibular gland immune function.

Characterization of microRNA profiles in the mammary gland tissue of dairy goats at the late lactation, dry period and late gestation stages.

MicroRNAs (miRNAs) play an important role in regulating mammary gland development and lactation. We previously analyzed miRNA expression profiles in Laoshan dairy goat mammary glands at the early (20 d postpartum), peak (90 d postpartum) and late lactation (210 d postpartum) stages. To further enrich and clarify the miRNA expression profiles during the lactation physiological cycle, we sequenced miRNAs in the mammary gland tissues of Laoshan dairy goats at three newly selected stages: the late lactation (240 d postpartum), dry period (300 d postpartum) and late gestation (140 d after mating) stages. We obtained 4038 miRNAs and 385 important miRNA families, including mir-10, let-7 and mir-9. We also identified 754 differentially expressed miRNAs in the mammary gland tissue at the 3 different stages and 6 groups of miRNA clusters that had unique expression patterns. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that GO terms such as mammary gland development (GO:0030879) and mammary gland morphogenesis (GO:0060443) and important signaling pathways, including the insulin signaling pathway (chx04910), hippo signaling pathway (chx04390) and estrogen signaling pathway (chx04915), were enriched. We screened miRNAs and potential target genes that may be involved in the regulation of lactation, mammary gland growth and differentiation, cell apoptosis, and substance transport and synthesis and detected the expression patterns of important genes at the three stages. These miRNAs and critical target genes may be important factors for mammary gland development and lactation regulation and potentially valuable molecular markers, which may provide a theoretical reference for further investigation of mammary gland physiology.

Nanoscale magnetic imaging of ferritins in a single cell.

The in situ measurement of the distribution of biomolecules inside a cell is one of the important goals in life science. Among various imaging techniques, magnetic imaging (MI) based on the nitrogen-vacancy (NV) center in diamond provides a powerful tool for the biomolecular research, while the nanometer-scale MI of intracellular proteins remains a challenge. Here, we use ferritin as a demonstration to realize the MI of endogenous proteins in a single cell using the NV center as the sensor. With the scanning, intracellular ferritins are imaged with a spatial resolution of ca. 10 nm, and ferritin-containing organelles are colocalized by correlative MI and electron microscopy. The approach paves the way for nanoscale MI of intracellular proteins.

Searching for an exotic spin-dependent interaction with a single electron-spin quantum sensor.

Searching for new particles beyond the standard model is crucial for understanding several fundamental conundrums in physics and astrophysics. Several hypothetical particles can mediate exotic spin-dependent interactions between ordinary fermions, which enable laboratory searches via the detection of the interactions. Most laboratory searches utilize a macroscopic source and detector, thus allowing the detection of interactions with submillimeter force range and above. It remains a challenge to detect the interactions at shorter force ranges. Here we propose and demonstrate that a near-surface nitrogen-vacancy center in diamond can be utilized as a quantum sensor to detect the monopole-dipole interaction between an electron spin and nucleons. Our result sets a constraint for the electron-nucleon coupling, [Formula: see text], with the force range 0.1-23 µm. The obtained upper bound of the coupling at 20 µm is [Formula: see text] < 6.24 × 10-15.