Antimicrobial indole alkaloids from Tabernaemontana corymbosa.

Four unreported monoterpene indole alkaloids, tabernaecorymines B-E (1-4), together with twenty-one known indole alkaloids (5-25) were obtained from the stem bark of Tabernaemontana corymbosa. Their structures and absolute configurations were elucidated by extensive spectroscopy, quantum chemical calculations, DP4+ probability analyses and Mo2(OAc)4-induced electronic circular dichroism experiment. The antibacterial and antifungal activities of these compounds were evaluated and some of them showed significant activity against Staphylococcus aureus,Bacillus subtilis, Streptococcus dysgalactiae and Candida albicans.

Dynamic Changes and Influencing Factors of Vegetation in the "Green Heart" Zone of the Chang-Zhu-Tan Urban Agglomeration during the Past 21 Years.

As a policy, protected green space in the rapidly developing the Chang-Zhu-Tan Urban Agglomeration is of great practical significance to study the vegetation changes and influencing factors in the Green Heart area. In this paper, data processing, grading and area statistics were carried out for the maximum value of normalized differential vegetation index (NDVI) from 2000 to 2020. Combined with Theil-Sen median trend analysis and Mann-Kendall, the change trend of long-time series NDVI was studied, and investigation of NDVI influencing factors, processes and mechanisms using geographical detectors. The results showed that: (1) The spatial distribution characteristics of NDVI in the study area were high in the middle and inlaid transition between adjacent grades. Except for the low grades, the distribution of NDVI in other grades was relatively scattered, and the overall trend of NDVI change was rising. (2) Population density was the main factor affecting NDVI changes, with an explanatory power of up to 40%, followed by elevation, precipitation and minimum temperature. (3) The influence of influencing factors on the change of NDVI was not the result of independent action of a single factor, but the result of the interaction between human factors and natural factors, and the factor combinations with greater interaction had significant differences in the spatial distribution of NDVI.

NPR3, transcriptionally regulated by POU2F1, inhibits osteosarcoma cell growth through blocking the PI3K/AKT pathway.

Natriuretic peptide receptor 3 (NPR3), mediates natriuretic peptides degradation, was reported to act as a tumor suppressor or promoter in some types of cancer. Previous studies showed that NPR3 was significantly decreased in osteosarcoma (OS) samples. However, the function and potential regulatory mechanism of NPR3 in OS development are unknown. By analyzing the protein expression of NPR3 in OS cell lines (n = 5) and human osteoblast cell line hFOB 1.19, we found that NPR3 expression was also significantly decreased in OS cells. The loss/gain-of-function analysis indicated that NPR3 overexpression observably decreased OS cell viability, arrested cell cycle, and induced apoptosis. However, NPR3 knockdown further enhanced the malignant phenotype of OS cells. Furthermore, NPR3 downregulation activated the PI3K/AKT pathway in OS cells, and the effects of NPR3 silencing on cell proliferation were reversed by the blockade of PI3K/AKT pathway. Of note, dual-luciferase reported assay and site-directed mutagenesis assay indicated that transcription factor POU domain class 2 transcription factor 1 (POU2F1) was proved to suppress NPR3 promoter activity by mainly binding to the -900 to -800 bp region of NPR3 promoter. Moreover, NPR3 overexpression inversed the promotion effect of POU2F1 on cell proliferation. In vivo experiments confirmed that NPR3 overexpression suppressed the growth of xenograft tumors. Taken together, the present study demonstrates that NPR3 may serve as a novel tumor suppressive factor through blocking the PI3K/AKT pathway and transcriptionally regulated by POU2F1.

Multiple morphologies from amphiphilic graft copolymers based on chitooligosaccharides as backbones and polycaprolactones as branches.

A new route to form multiple morphologies was outlined using amphiphilic graft copolymers with interesting biological and pharmacological properties by proper adjustment of backbone and graft chain length.

Template synthesis of functionalized polystyrene in ordered silicate channels.

A mesostructured nanocomposite was fabricated by using a novel electroactive, polymerizable surfactant as a template in a sol-gel process, and the first example of well-resolved polystyrene with redoxactive functional group synthesized in silicate matrices was provided.