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The objective of this study was to examine the effects of varying levels of dietary chitosan supplementation on mitigating cadmium stress and its influence on growth performance, serum biochemical indices, antioxidant capacity, immune response, inflammatory response, and the expression of related genes in juvenile Genetically Improved Farmed Tilapia (GIFT, Oreochromis niloticus). Five groups of juvenile tilapias (initial body weight 21.21 ± 0.24 g) were fed five diets with different levels (0%, 0.5%, 1.0%, 1.5%, and 2.0%) of chitosan supplementation for 60 days under cadmium stress (0.2 mg/L Cd2+). The findings indicated that, compared with the 0% chitosan group, dietary chitosan could significantly increase (p < 0.05) the final weight (Wf), weight gain rate (WGR), specific growth rate (SGR), daily growth index (DGI), and condition factor (CF), while the feed conversion ratio (FCR) expressed the opposite trend in juvenile GIFT. Dietary chitosan could significantly increase (p < 0.05) the activities (contents) of cholinesterase (CHE), albumin (ALB), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), acid phosphatase (ACP), and lysozyme (LZM), while glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), and complement 3 (C3) in the serum of juvenile GIFT expressed the opposite trend. Dietary chitosan could significantly increase (p < 0.05) the activities of superoxide dismutase (SOD) and catalase (CAT) and significantly decrease (p < 0.05) the activities (contents) of glutathione S-transferase (GST), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) in the serum of juvenile GIFT. Dietary chitosan could significantly increase (p < 0.05) the activities (contents) of CAT, GST, GSH-Px, and total antioxidant capacity (T-AOC) and significantly decrease (p < 0.05) the contents of MDA in the liver of juvenile GIFT. Dietary chitosan could significantly increase (p < 0.05) the activities (contents) of SOD, GSH-Px, T-AOC, Na+-K+-ATPase, and Ca2+-ATPase and significantly decrease (p < 0.05) the activities (contents) of CAT, GST, and MDA in the gills of juvenile GIFT. Dietary chitosan could significantly up-regulate (p < 0.05) the gene expression of cat, sod, gst, and gsh-px in the liver of juvenile GIFT. Dietary chitosan could significantly up-regulate (p < 0.05) the gene expression of interferon-γ (inf-γ) in the gills and spleen and significantly down-regulate (p < 0.05) the gene expression of inf-γ in the liver and head kidney of juvenile GIFT. Dietary chitosan could significantly down-regulate (p < 0.05) the gene expression of interleukin-6 (il-6), il-8, and tumor necrosis factor-α (tnf-α) in the liver, gills, head kidney, and spleen of juvenile GIFT. Dietary chitosan could significantly up-regulate (p < 0.05) the gene expression of il-10 in the liver, gills, head kidney, and spleen of juvenile GIFT. Dietary chitosan could significantly up-regulate (p < 0.05) the gene expression of transforming growth factor-ß (tgf-ß) in the liver and significantly down-regulate (p < 0.05) the gene expression of tgf-ß in the head kidney and spleen of juvenile GIFT. In conclusion, dietary chitosan could mitigate the impact of cadmium stress on growth performance, serum biochemical indices, antioxidant capacity, immune response, inflammatory response, and related gene expression in juvenile GIFT. According to the analysis of second-order polynomial regression, it was found that the optimal dietary chitosan levels in juvenile GIFT was approximately 1.42% to 1.45%, based on its impact on Wf, WGR, SGR, and DGI.
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The present study aimed to compare the nutritional effects of cholesterol, bile acids, and combination of cholesterol with bile acids in plant-based diets on juvenile genetically improved farmed tilapia (GIFT; Oreochromis niloticus). The isonitrogenous (321 g/kg crude protein) and isolipidic (76 g/kg crude fat) diets (Con diet) were based on plant protein sources, which included corn gluten meal, soybean meal, cottonseed meal and rapeseed meal. The Con diet was supplemented with 12 g/kg cholesterol (CHO diet), 0.2 g/kg bile acids (BAs diet), a combination of 12 g/kg cholesterol and 0.2 g/kg bile acids (CHO-BAs diet), respectively. Each diet was fed to three tanks in an indoor recirculating aquaculture system for 9 weeks. Results showed that compared to the Con group, fish had a higher weight gain rate, hepatosomatic index, and a lower feed conversion ratio in the CHO-BAs group. The highest levels of whole-fish fat and ash were found in the Con group. Serum parameters, including activities of alanine aminotransferase (ALT) and aspartate transaminase (AST), along with levels of glucose (GLU) and triglyceride (TG) except for total cholesterol (TCHO), were lower in the CHO, BAs, and CHO-BAs groups than those in the Con group (P < 0.001). Histological examination revealed that fish in the Con group exhibited severe hepatocyte vacuolization and diminished hepatocyte proliferation. Gene expression analysis indicated that the transcriptional levels of bile acid metabolism-related genes (including fxr, fgf19, bsep) were up-regulated in the CHO-BAs group (P < 0.05), whereas cholesterol metabolism-related genes (acly and hmgcr) were down-regulated in both CHO and CHO-BAs groups (P < 0.001). Moreover, UPLC-MS/MS analysis revealed that the higher taurine-conjugated bile acids (T-BAs), followed by free bile acids (Free-BAs) and glycine (G-BAs) were determined in tilapia bile. Among these, taurochenodeoxycholic bile acid was the predominant bile acid. Dietary bile acids supplementation also increased the proportion of T-BAs (tauro ß-muricholic acid and taurodehydrocholic acid) while decreasing Free-BAs in the fish bile. In conclusion, the incorporation of cholesterol with bile acids into plant-based diets can effectively reduce cholesterol uptake, suppress bile acids synthesis, enhance bile acids efflux, and promote hepatocyte proliferation, which is helpful for maintaining the normal liver morphology in tilapia, and thus improving its growth performance.
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Nile tilapia (Oreochromis niloticus) is a worldwide farmed fish and has been widely used for the study on comparative immunology in teleosts. It is well known that cadmium (Cd) can cause a variety of adverse effects in fish. However, data on the effects of Cd in fish liver and the defensive mechanisms of these effects using transcriptome approach are relatively scarce to date. In this study, by using an RNA sequencing approach, the gene expression profiling was performed in livers of tilapia exposed to 0 (control), 50, 100, and 200⯵g/L of Cd for 2 months. The results showed that exposure to 50⯵g/L Cd altered the expressions of 911 genes, while exposure to 100 and 200⯵g/L Cd resulted in 4318 and 3737 differentially expressed genes compared to the control. Weighted correlation network analysis (WGCNA) and gene ontology (GO) analysis identified a 14-gene network linked to the immune system development. Further, in a fuzzy analysis, the GO term immune system development was enriched in cluster 3, and gene expression decreased with increasing Cd levels in a concentration-dependent manner. The qPCR and RNA-seq results identified 4 genes, i.e., dnmt3bb.1, sf3b1, SMARCAL1, and zap70, as convenient potential biological indicators for detecting waterborne Cd. The present results help systematically understand the effects of Cd on the hepatic transcriptome in tilapia.
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Ciclídeos , Tilápia , Animais , Ciclídeos/metabolismo , Cádmio/toxicidade , Cádmio/metabolismo , Perfilação da Expressão Gênica , Tilápia/metabolismo , TranscriptomaRESUMO
Indoor decoration generates a large number of volatile organic compounds (VOCs), which are simultaneously released from different paints. Nevertheless, the interaction mechanism of pollutant diffusion from multisource building materials (such as primer and finish) needs to be examined. In this paper, a multisource mass transfer model for VOC emissions from different combinations of paints is established, and the analytical solution is derived. The finite difference method is used to simulate the experimental results of VOC release in the environmental chambers, and its convergence and stability are verified. Using the optimization parameters of the single-source model and the law of conservation of mass, the key parameters of the multisource mass transfer model are obtained. The results show that the established model is in excellent agreement with both experimental data and literature data. In addition, the Little number Lt is used to analyse the change trend from the initial released concentration in the single-source and multisource models.
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Ammonia is a key risk factor in intensive aquaculture systems. This experiment is aimed at investigating the influence of dietary protein levels on genetically improved farmed tilapia (GIFT, Oreochromis niloticus) under chronic ammonia stress. GIFT juveniles of 4.00 ± 0.55 g were exposed to high ammonia level at 0.88 mg/L and fed with six diets comprising graded protein levels at 22.64%, 27.26%, 31.04%, 35.63%, 38.47%, and 42.66% for 8 weeks. The fish in negative control was fed the diet with 31.04% protein in normal water (0.02 mg ammonia/L water). Our results showed that high ammonia exposure (0.88 mg/L) caused significant decrease in fish growth performance, hematological parameters, liver antioxidant enzymes (catalase and glutathione peroxidase), and gill Na+- and K+-dependent adenosine triphosphatase (Na+/K+-ATP) activity. When fish were under high ammonia exposure, the weight gain rate, special growth rate, feed efficiency, and survival rate elevated significantly with dietary protein supplementation increase to 35.63%, whereas protein efficiency ratio, hepatosomatic index, and viscerosomatic index showed a decreased tendency. Dietary protein administration significantly enhanced crude protein but reduced crude lipid contents in the whole fish. Fish fed diets with 35.63%-42.66% protein had higher red blood cell counts and hematocrit percentage than fish fed 22.64% protein diet. The values of serum biochemical indices (lactate dehydrogenase, aspartate aminotransferase, and alanine aminotransferase), hepatic antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase), and gill Na+/K+-ATP activity were all elevated with the increment of dietary protein. Moreover, histological analysis indicated that dietary protein administration could prevent the ammonia-induced damages in fish gill, kidney, and liver tissues. Based on weight gain rate as a response criterion, the optimal dietary protein requirement for GIFT juveniles under chronic ammonia stress was 37.9%.
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Volatile organic compounds (VOCs) emitted from porous wood-based panels with fractal structure severely pollute indoor environment. Different from previous studies which the diffusion type of VOC in building materials is attributed to Fick diffusion, VOC emission from porous wood-based panels belongs to Knudsen diffusion is firstly determined by comparing the pore diameter of internal channel with VOC molecular free path in this paper. Therefore, a time fractional mass transfer model related to the fractal dimension has been proposed to analyze Knudsen diffusion characteristics firstly. This model considers areal porosity has an impact on surface emission. Analytical solution of the present model is obtained for the first time. Furthermore, it is proved that the finite difference scheme is solvable, unconditionally stable, and convergent, and numerical simulation result and experimental data match well. Moreover, the influences of the fractal dimension df, areal porosity ε, and delay time parameter λ on VOC emission are demonstrated and analyzed; results suggest that the higher ε and df, and lower λ promote VOC emission, which can provide guidance for improving indoor air quality.
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Poluição do Ar em Ambientes Fechados , Compostos Orgânicos Voláteis , Madeira/química , Compostos Orgânicos Voláteis/análise , Porosidade , Materiais de Construção , Poluição do Ar em Ambientes Fechados/análiseRESUMO
Indoor decoration causes a large amount of formaldehyde to be concurrently released from different surfaces of wood-based panels. However, this phenomenon has rarely been described with two- or three-dimensional mass transfer models. In this paper, multidimensional mass transfer models of formaldehyde emissions originating from different surfaces of building materials are first established. Moreover, mass balance integral equation is introduced. Experiments of formaldehyde emissions in the environmental chambers are numerically simulated by combining the parameter estimation method, composite Simpson formula and finite difference method, whose convergence and stability are verified. The results indicated that the proposed models suitably agreed with the experimental and literature data. Furthermore, the multidimensional mass transfer model confirmed that formaldehyde released from sides should not be ignored. Compared with the one-dimensional model, the three-dimensional model can more accurately simulate the release data of formaldehyde from wood-based panels. Subsequently, the variation trend between the chemical reaction formation rate and physical diffusion rate of formaldehyde is revealed.
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Formaldeído , Madeira , Materiais de Construção , DifusãoRESUMO
(1) Background: Singapore grouper iridovirus (SGIV) can cause extensive fish deaths. Therefore, developing treatments to combat virulent SGIV is of great economic importance to address this challenge to the grouper aquaculture industry. Green tea is an important medicinal and edible plant throughout the world. In this study, we evaluated the use of green tea components against SGIV infection. (2) Methods: The safe working concentrations of green tea components were identified by cell viability detection and light microscopy. Additionally, the antiviral activity of each green tea component against SGIV infection was determined with light microscopy, an aptamer (Q5c)-based fluorescent molecular probe, and reverse transcription quantitative PCR. (3) Results: The safe working concentrations of green tea components were green tea aqueous extract (GTAE) ≤ 100 µg/mL, green tea polyphenols (TP) ≤ 10 µg/mL, epigallocatechin-3-gallate (EGCG) ≤ 12 µg/mL, (-)-epigallocatechin (EGC) ≤ 10 µg/mL, (-)-epicatechin gallate (EGC) ≤ 5 µg/mL, and (-)-epicatechin (EC) ≤ 50 µg/mL. The relative antiviral activities of the green tea components determined in terms of MCP gene expression were TP > EGCG > GTAE > ECG > EGC > EC, with inhibition rates of 99.34%, 98.31%, 98.23%, 88.62%, 73.80%, and 44.31%, respectively. The antiviral effect of aptamer-Q5c was consistent with the results of qPCR. Also, TP had an excellent antiviral effect in vitro, wherein the mortality of fish in only the SGIV-injection group and TP + SGIV-injection group were 100% and 11.67%, respectively. (4) Conclusions: In conclusion, our results suggest that green tea components have effective antiviral properties against SGIV and may be candidate agents for the effective treatment and control of SGIV infections in grouper aquaculture.
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Bass , Infecções por Vírus de DNA , Doenças dos Peixes , Iridovirus , Ranavirus , Animais , Antivirais/farmacologia , Antivirais/uso terapêutico , Infecções por Vírus de DNA/veterinária , Iridovirus/genética , Ranavirus/fisiologia , CháRESUMO
Singapore grouper iridovirus (SGIV) causes high economic losses in mariculture. Effective drugs for managing SGIV infection are urgently required. Medicinal plant resources are rich in China. Medicinal plants have a long history and significant curative effects in the treatment of many diseases. Reverse-transcription quantitative real-time PCR is the most commonly used method for detecting virus infection and assessing antiviral efficacy with high accuracy. However, their applications are limited due to high reagent costs and complex time-consuming operations. Aptamers have been applied in some biosensors to achieve the accurate detection of pathogens or diseases through signal amplification. This study aimed to establish an aptamer-based high-throughput screening (AHTS) model for the efficient selection and evaluation of medicinal plants components against SGIV infection. Q2-AHTS is an expeditious, rapid method for selecting medicinal plant drugs against SGIV, which was characterized as being dram, high-speed, sensitive, and accurate. AHTS strategy reduced work intensity and experimental costs and shortened the whole screening cycle for effective ingredients. AHTS should be suitable for the rapid selection of effective components against other viruses, thus further promoting the development of high-throughput screening technology.
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Aptâmeros de Nucleotídeos , Bass , Infecções por Vírus de DNA , Doenças dos Peixes , Ranavirus , Animais , Antivirais/farmacologia , Aptâmeros de Nucleotídeos/farmacologia , Ensaios de Triagem em Larga EscalaRESUMO
Circular RNA (circRNA) is an endogenous biomolecule in eukaryotes. It has tissue- and cell-specific expression patterns and can act as a microRNA sponge or competitive endogenous RNA. Although circRNA has been found in several species in recent years, the expression profiles in fish gonad are still not fully understood. We detected the expression of circRNA in the ovary, testis, and sex-changed gonad of tilapia by high-throughput deep sequencing, and circRNA-specific computing tools. A total of 20,607 circRNAs were obtained, of which 141 were differentially expressed in the testis and ovary. Among these circRNAs, 135 circRNAs were upregulated and 6 circRNAs were downregulated in female fish. In addition, GO annotation and KEGG pathway analysis of the host genes of circRNAs indicated that these host genes were mainly involved in adherens junction, androgen production, and reproductive development, such as ZP3, PLC, delta 4a, ARHGEF10, and HSD17b3. It is worth noting that we found that circRNAs in tilapia gonads have abundant miRNA-binding sites. Among them, 935 circRNAs have a regulatory effect on miR-212, 856 circRNAs have a regulatory effect on miR-200b-3p, and 529 circRNAs have a regulatory effect on miR-200b-5p. Thus, our findings provide a new evidence for circRNA-miRNA networks in the gonads in tilapia.
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Dantron (DA), a kind of polyhydric anthraquinone and one of the bio-active ingredient in Rheum officinale was chosen as the ligand to coordinate with the bio-active copper(II) ion to achieve its antibacterial copper(II) complex, DA-Cu. The coordination structure of DA-Cu, both in the crystal state and solution state, was studied by spectroscopy and X-ray single-crystal diffraction analysis. The inhibition zone, MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration) values regarding the in vitro antibacterial activity of DA-Cu towards Flavobacterium columnar, which causes the bacterial gill-rot disease on fish, were significant and specific. DA-Cu in vivo acute toxicity on zebrafish and tilapia was evaluated, suggesting that the higher dose of DA-Cu than 0.1 mg/mL might give potential toxicity. The further therapeutic effect of DA-Cu on the tested tilapia challenged by Flavobacterium columnar was also studied, which showed its clear advantage (including the survival rate, relative weight gain rate, and feed conversion ratio) over DA and the positive control, Sanhuang San, at a much lower dosage of 0.025 mg/mL.
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Infecções Bacterianas , Tilápia , Animais , Antraquinonas , Antibacterianos/química , Antibacterianos/farmacologia , Cobre/química , Cobre/farmacologia , Flavobacterium , Brânquias , Peixe-ZebraRESUMO
Lycium barbarum polysaccharide (LBP) is one of the main active ingredients in the fruit of L. barbarum L. It has been used as herbal medicine for thousands of years in China. In this study, Nile tilapia (Oreochromis niloticus) was taken as the research object. After feeding tilapia with 5 different doses of LBP (0 mg/kg, 500 mg/kg, 1000 mg/kg, 1500 mg/kg, 2000 mg/kg) for 55 d, it was found that LBP could promote the growth of tilapia, and this effect was the strongest at Group 1500 mg/kg. Apoptosis analysis in the liver and spleen showed that dietary supplementation with 1000 mg/kg LBP had the best protective effect on the spleen and liver in tilapia. Combined transcriptomics and metabolomics of the spleen in tilapia at Group 0 mg/kg and 1000 mg/kg showed that the differentially expressed genes (DEGs) such as NT5C2L1, pmm1, FasL and the differentially metabolites such as xanthine, dGMP, guanine and glutamate were mainly concentrated in signaling pathways such as Purine metabolism and FoxO signaling pathway. In conclusion, LBP regulates the metabolic waste levels of tilapia mainly through Purine metabolism and the FoxO signaling pathway, thereby inhibiting cell apoptosis, improving the utilization of nutrients, and promoting the growth of tilapia. This study not only provides a theoretical basis for the application of LBP in aquatic animals but also provides useful information for the healthy development of the aquaculture.
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Ciclídeos , Lycium , Animais , Apoptose , Ciclídeos/genética , Medicamentos de Ervas Chinesas , Lycium/metabolismo , Metabolômica , TranscriptomaRESUMO
DGAT2 (acyl CoA:diacylglycerol acyltransferase 2) is a key and rate-limiting enzyme that catalyzes the final step of triglyceride (TG) synthesis. In this study, hybrid tilapia were generated from Nile tilapia (â) and blue tilapia (â) crossing. The TG content levels in the liver of these tilapia were measured. The results showed that the TG content was higher in the hybrid tilapia. In addition, protein and mRNA expression levels in the tilapia livers were determined. Higher hepatic mRNA and protein expression of DGAT2 in the hybrid fish was found. A luciferase reporter assay with HEK293T cells revealed that miRNA-19a-5p targeted the 3'UTR of DGAT2, suggesting a direct regulatory mechanism. Using qRT-PCR, we found that DGAT2 mRNA levels had a negative correlation with miRNA-19a-5p expression in Nile tilapia and hybrid. Taken together, these findings provide evidence that miRNA-19a-5p is involved in TG synthesis in the regulation of lipid metabolism in tilapia.
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MicroRNAs , Tilápia , Animais , Diacilglicerol O-Aciltransferase/genética , Diacilglicerol O-Aciltransferase/metabolismo , Feminino , Células HEK293 , Humanos , Masculino , MicroRNAs/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Tilápia/genética , Tilápia/metabolismo , Triglicerídeos/metabolismoRESUMO
Volatile organic compounds (VOCs) concentrations of dry building materials can significantly affect the quality of indoor air. In this paper, a spatial fractional diffusion model is established by introducing fractional Fick's law, based on the fact that the pore structure of porous building materials has a strongly impact on VOCs diffusion. Furthermore, the areal porosity of the material is drawn into the convection mass transfer equation. The relevant parameters are estimated by a kind of optimization algorithm. The fractional nonlinear equations are tackled by the finite difference method combined with L2-algorithm. Results indicate that spatial fractional diffusion model agrees better with the experimental data than Deng and Kim's model. The spatial fractional diffusion model is used to describe the formaldehyde concentration in the particleboard according to the experimental data. Taking formaldehyde as a common substance, the influences of physical parameters on VOCs diffusion are predicted by this model. The results of numerical simulation demonstrate that the diffusion is consistent with the actual situation.
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In this study, the effect of Lycium barbarum polysaccharides (LBP) on immunological parameters, apoptosis, and growth performance of Nile tilapia (Oreochromis niloticus) was investigated. Dietary supplementation with LBP significantly increased complement 3 (C3) activity and promoted interleukin IL-1ß gene expression in spleen tissue, significantly reduced apoptosis in spleen tissue, increased the specific growth rate (SGR), relative length gain (LG), and relative weight gain (WG) of Nile tilapia. However, dietary supplementation with LBP did not have a significant effect on serum alkaline phosphatase (AKP), malondialdehyde (MDA), and superoxide dismutase (SOD), blood constituents, apoptosis, or gene expression of IL-1ß in liver tissue. Overall, the results showed that dietary supplementation with LBP increased the nonspecific immunity of Nile tilapia and reduced the apoptosis rate to promote growth and development. Thus, LBP has potential for use as a new immunostimulant in aquaculture.
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Apoptose/efeitos dos fármacos , Ciclídeos/crescimento & desenvolvimento , Ciclídeos/imunologia , Suplementos Nutricionais , Medicamentos de Ervas Chinesas/administração & dosagem , Ração Animal , Animais , Aquicultura , Complemento C3/imunologia , Doenças dos Peixes/imunologia , Interleucina-1beta/genética , Interleucina-1beta/imunologiaRESUMO
This study investigated the molecular mechanisms involved in ovarian transcriptomic responses in Nile tilapia under different photoperiod regimes. Histological analysis indicated that ovarian development was significantly affected by photoperiod. The photoperiods tested were as follows: LD (12 h light:12 h dark), LL (24 h light:0 h dark), and DD (0 h light:24 h dark). The longer photoperiod (LL) was shown to induce ovary development earlier than LD and DD. Next, ovary transcriptome levels were sequenced and analyzed. These data indicated that 988, 992, and 1,036 differentially expressed genes (DEGs) were detected by comparing LD-LL, LD-DD, and LL-DD. A number of genes that may be involved in photoperiod-specific regulation of ovarian development were observed. These findings may be useful for investigating the molecular mechanisms underlying light-induced ovarian development in Nile tilapia.
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The concentrations, congener profiles and spatial distribution of 13 phthalate esters (PAEs) in the freshwater fish ponds in the Pearl River Delta (PRD) region were investigated in water and sediment samples collect from 22 sites during Jul. 2016-Sept. 2017. The di-2-ethylhexyl phthalate (DEHP) was the predominant compounds in both water and sediment samples, accounting for 70.1% and 66.1% of ∑PAEs, respectively. The DEHP concentrations in the water samples collected from the sites of Zhongshan (35.7⯵g/L), Jingmen (17.3⯵g/L) and Nanhai (14.2⯵g/L) were higher than that collected from other sampling sites (p <0.05), and exceed the Chinese environmental quality standards for surface water (DEHP, 8.00⯵g/L). The concentrations of ΣPAEs (mean and median were 11.8â¯mg/kg dw and 7.95â¯mg/kg dw) in sediment was higher than that in sediment of river and estuary in the PRD region (p <0.05). The median concentrations of DEHP and di-n-butyl phthalate (DBP) exceeded recommend environmental risk limit (ERL) that posed a potential risk to the aquaculture fish pond environment in the PRD.
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Aquicultura , Monitoramento Ambiental , Ésteres/análise , Ácidos Ftálicos/análise , Poluentes Químicos da Água/análise , China , Dibutilftalato/análise , Dietilexilftalato/análise , Estuários , Lagoas , Rios , Alimentos MarinhosRESUMO
Nonadditive expression contributes to heterosis in hybrids. In this study, the expression profiles of twelve lipid metabolism pathway-related genes were investigated in the intestine of Nile tilapia (Oreochromis niloticus) â × blue tilapia (Oreochromis aureus) â hybrid. The expression of genes from the hybrid were assigned to nonadditive and additive expression pattern groups and compared with expression patterns from Nile tilapia and blue tilapia. In the intestine of the hybrid, apoA4B was expressed at intermediate levels, but apoB and MTP were assigned to ELD-B and ELD-N categories, respectively. The LPL and LRP1 showed transgressive up-regulation in the hybrid, but LDLR was assigned to the ELD-B category. For fatty acid uptake related genes, only FABP11a was categorized as nonadditive expression with transgressive up-regulation, while CD36 and FABP3 were categorized as additive expression in the intestine of the hybrid. Two genes in triacylglycerol metabolism, namely, FAS and DGAT2, showed transgressive up-regulation in the hybrid. Most of the genes analyzed in the present study showed nonadditive expression (8 in 12), and five genes showed transgressive up-regulation. These results indicated that the stimulation of lipid metabolism in the hybrid compared to that of its parents. The hyperactive expression of these genes in the hybrid may be associated with the growth and lipid usage vigor.
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Vigor Híbrido/genética , Metabolismo dos Lipídeos/genética , Tilápia/genética , Ração Animal/análise , Animais , Ciclídeos/genética , Ácidos Graxos/metabolismo , Feminino , Intestinos , Lipídeos , Masculino , Transcriptoma/genéticaRESUMO
Hypoxia is as an endocrine disruptor, and, in crustaceans, the energy metabolic consequences of hypoxia in the brain tissue are still poorly understood. We combined gas chromatography-mass spectrometry (GC-MS)-based metabolomic analysis and high-throughput RNA sequencing to evaluate the metabolic effects and subjacent regulatory pathways in the brain tissue of the male Oriental river prawn (Macrobrachium nipponense) in response to hypoxia and reoxygenation. We recorded LC50 and heartbeats per minute of male M. nipponense juveniles. Hypoxia resulted in the generation of reactive oxygen species in the brain cells and alterations in gene expression and metabolite concentrations in the prawn brain tissue in a time-dependent manner. The transcriptomic analyses revealed specific changes in the expression of genes associated with metabolism pathways, which was consistent with the changes in energy metabolism indicated by the GC-MS metabolomic analysis. Quantitative real-time polymerase chain reaction and western blot confirmed the transcriptional induction of these genes because of hypoxia. The lactate levels increased significantly during hypoxia and decreased to normal after reoxygenation; this is consistent with a shift towards anaerobic metabolism, which may cause metabolic abnormalities in the brain tissue of M. nipponense. Overall, these results are consistent with metabolic disruption in the brain of M. nipponense exposed to hypoxia and will help in understanding how crustacean brain tissue adapts and responds to hypoxia and reoxygenation.
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Perfilação da Expressão Gênica , Metaboloma/fisiologia , Palaemonidae/fisiologia , Anaerobiose , Animais , Encéfalo/metabolismo , Metabolismo Energético , Eutrofização , Regulação da Expressão Gênica , Hipóxia/metabolismo , Masculino , MetabolômicaRESUMO
Hypoxia represents a major physiological challenge for prawns and is a problem in aquaculture. Therefore, an understanding of the metabolic response mechanism of economically important prawn species to hypoxia and re-oxygenation is essential. However, little is known about the intrinsic mechanisms by which the oriental river prawn Macrobrachium nipponense copes with hypoxia at the metabolic level. In this study, we conducted gas chromatography-mass spectrometry-based metabolomics studies and assays of energy metabolism-related parameters to investigate the metabolic mechanisms in the hepatopancreas of M. nipponense in response to 2.0 O2/L hypoxia for 6 and 24 h, and reoxygenation for 6 h following hypoxia for 24 h. Prawns under hypoxic stress displayed higher glycolysis-related enzyme activities and lower mRNA expression levels of aerobic respiratory enzymes than those in the normoxic control group, and those parameters returned to control levels in the reoxygenated group. Our results showed that hypoxia induced significant metabolomic alterations in the prawn hepatopancreas within 24 h. The main metabolic alterations were depletion of amino acids and 2-hydroxybutanoic acid and accumulation of lactate. Further, the findings indicated that hypoxia disturbed energy metabolism and induced antioxidant defense regulation in prawns. Surprisingly, recovery from hypoxia (i.e., reoxygenation) significantly affected 25 metabolites. Some amino acids (valine, leucine, isoleucine, lysine, glutamate, and methionine) were markedly decreased compared to the control group, suggesting that increased degradation of amino acids occurred to provide energy in prawns at reoxygenation conditions. This study describes the acute metabolomic alterations that occur in prawns in response to hypoxia and demonstrates the potential of the altered metabolites as biomarkers of hypoxia.