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Objective To detect the transferred vascular endothelial growth factor (VEGF)165 gene expression in rhesus autologous bone marrow mesenchymal stem cells (MSCs), and to explore the functional viability of transgenic MSCs. Methods MSCs from rhesus bone were isolated by Ficoll, which were used to detect the phenotype. After the culturing, the expression vector pcDNA-eGFP-VEGF165 was transfected into bone marrow MSCs. Fluorescence microscope and flow cytometry were used to detect the enhanced green fluorescent protein (eGFP) expression. At the same time, the phenotype in transfected MSCs was also indentified. The VEGF165 expression level was detected by RT-PCR. Results The highly purified MSCs were collected successfully. The transfected MSCs and daughter cells showed expressions of eGFP and VEGF165, which also remained the characteristics of MSCs. Conclusion The VEGF165 gene that is transfected into MSCs can maintain characteristics of MSCs, and stably express foreign genes.
RESUMO
Objective To observe the effects of nourishing-kidney herbs on rat model with glucocorticoid-induced osteoporosis.Methods 60 Wistar rats male and female half and half were randomly divided into a normal group,a model group and a nourishing-kidney group.By intramuscular injection of dexamethasone (2.5 mg/kg) twice a week to replicate osteoporosis rat model.All groups were treated for 9 weeks.Detected the BMD of femur in vitro and determined the bone metabolism marker in serum by biochemical process.Results The BMD decreased obviously (0.109± 0.007)g/cm2 and the content of TRAP in serum increased evidently (9.96± 1.15) μg/ml in the model group.In the nourishing-kidney group,the BMD was up-regulated (0.116 ± 0.007)g/cm2,and TRAP down-regulated(5.76 ± 0.85)μg/ml.Conclusion Intramuscular injection of dexamethasone can induce GIO rat model,and nourishing-kidney herbs have the effect of anti-osteoporosis.
