RESUMO
The ultimate aim of nuclear reprogramming is to provide stem cells or differentiated cells from unrelated cell types as a cell source for regenerative medicine. A popular route towards this is transcription factor induction, and an alternative way is an original procedure of transplanting a single somatic cell nucleus to an unfertilized egg. A third route is to transplant hundreds of cell nuclei into the germinal vesicle (GV) of a non-dividing Amphibian meiotic oocyte, which leads to the activation of silent genes in 24â h and robustly induces a totipotency-like state in almost all transplanted cells. We apply this third route for potential therapeutic use and describe a procedure by which the differentiated states of cells can be reversed so that totipotency and pluripotency gene expression are regained. Differentiated cells are exposed to GV extracts and are reprogrammed to form embryoid bodies, which shows the maintenance of stemness and could be induced to follow new directions of differentiation. We conclude that much of the reprogramming effect of eggs is already present in meiotic oocytes and does not require cell division or selection of dividing cells. Reprogrammed cells by oocytes could serve as replacements for defective adult cells in humans.
Assuntos
Oócitos , Transplante de Células-Tronco , Adulto , Animais , Humanos , Núcleo Celular , Anfíbios , Reprogramação Celular , MamíferosRESUMO
An important characteristic of cell differentiation is its stability. Only rarely do cells or their stem cell progenitors change their differentiation pathway. If they do, it is often accompanied by a malfunction such as cancer. A mechanistic understanding of the stability of differentiated states would allow better prospects of alleviating the malfunctioning. However, such complete information is yet elusive. Earlier experiments performed in Xenopus oocytes to address this question suggest that a cell may maintain its gene expression by prolonged binding of cell type-specific transcription factors. Here, using DNA competition experiments, we show that the stability of gene expression in a nondividing cell could be caused by the local entrapment of part of the general transcription machinery in transcriptionally active regions. Strikingly, we found that transcriptionally active and silent forms of the same DNA template can stably coexist within the same nucleus. Both DNA templates are associated with the gene-specific transcription factor Ascl1, the core factor TBP2, and the polymerase II (Pol-II) ser5 C-terminal domain (CTD) phosphorylated form, while Pol-II ser2 CTD phosphorylation is restricted to the transcriptionally dominant template. We discover that the active and silent DNA forms are physically separated in the oocyte nucleus through partition into liquid-liquid phase-separated condensates. Altogether, our study proposes a mechanism of transcriptional regulation involving a spatial entrapment of general transcription machinery components to stabilize the active form of a gene in a nondividing cell.
Assuntos
DNA/genética , Regulação da Expressão Gênica , Oócitos/metabolismo , Transcrição Gênica , Animais , Diferenciação Celular , DNA/metabolismo , Humanos , Oócitos/citologia , Fosforilação , RNA Polimerase II/metabolismo , Moldes Genéticos , XenopusRESUMO
Proper function of the body is maintained by an intricate interaction and communication among cells. during the animal development how these cells are formed and maintained is an important yet elusive. Understanding of how cells such as muscle and nerve cells maintain their identities would enable us to control the diseases which include malfunctioning in cellular identities such as cancer. In this article, we describe how the concept of formation and maintenance of cell identities has changed over the last 100 years. We will also briefly describe our current experimental work which includes transcriptional dynamics, and protein-protein interaction and how they are bringing new molecular insights. We also describe liquid-liquid phase separation as a potential new mechanism for the stability of gene expression in the non dvididng specialised cells of Xenopus oocytes.
Assuntos
Regulação da Expressão Gênica no Desenvolvimento , Oócitos/citologia , Oócitos/metabolismo , Xenopus laevis/embriologia , Xenopus laevis/genética , Animais , Divisão Celular Assimétrica/genética , Diferenciação Celular/genética , Feminino , Células Musculares/metabolismo , Neurônios/metabolismo , Ovoviviparidade/genética , Mapas de Interação de Proteínas/genética , Transcrição Gênica/genética , Xenopus laevis/metabolismoRESUMO
The aim of this short review is to comment on the advantages of injecting purified molecules into a normal living cell as a complement to the constitution of a cell-free system for analyzing the function of cell components. We emphasize here that the major difference is that, by injection, most components of a cell are maintained at their normal concentration, which is difficult, even if at all possible, to achieve in a cell-free system. We exemplify the benefits of a cell injection system by the efficiency and long duration of DNA transcription by RNA polymerase II, as used by most genes, and by the widespread success of injecting purified messenger RNA for protein synthesis. The most recent work using cell injection also gives a new understanding of a long lasting transcription factor residence on its DNA or chromatin not shown by other procedures. Lastly, we re-visit an old idea that transcription factors that guide cell fate may be stably bound to DNA or chromatin, except at S-phase or mitosis in the cell cycle, when they can undergo exchange with equivalent molecules in the cell.
Assuntos
Células/metabolismo , Regulação da Expressão Gênica , Injeções , Animais , Sistema Livre de Células , Humanos , Fatores de Transcrição/metabolismo , Transcrição GênicaRESUMO
Some lineage-determining transcription factors are overwhelmingly important in directing embryonic cells to a particular differentiation pathway, such as Ascl1 for nerve. They also have an exceptionally strong ability to force cells to change from an unrelated pathway to one preferred by their action. Transcription factors are believed to have a very short residence time of only a few seconds on their specific DNA or chromatin-binding sites. We have developed a procedure in which DNA containing one copy of the binding site for the neural-inducing factor Ascl1 is injected directly into a Xenopus oocyte nucleus which has been preloaded with a limiting amount of the Ascl1 transcription factor protein. This is followed by a further injection of DNA as a competitor, either in a plasmid or in chromosomal DNA, containing the same binding site but with a different reporter. Importantly, expression of the reporter provides a measure of the function of the transcription factor in addition to its residence time. The same long residence time and resistance to competition are seen with the estrogen receptor and its DNA response elements. We find that in this nondividing oocyte, the nerve-inducing factor Ascl1 can remain bound to a specific chromatin site for hours or days and thereby help to stabilize gene expression. This stability of transcription factor binding to chromatin is a necessary part of its action because removal of this factor causes discontinuation of its effect on gene expression. Stable transcription factor binding may be a characteristic of nondividing cells.
Assuntos
Fatores de Transcrição Hélice-Alça-Hélice Básicos/metabolismo , Cromatina/metabolismo , Animais , Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Sítios de Ligação , Cromatina/genética , DNA/genética , DNA/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Oócitos/crescimento & desenvolvimento , Oócitos/metabolismo , Ligação Proteica , Xenopus laevis/embriologia , Xenopus laevis/genética , Xenopus laevis/metabolismoRESUMO
Unlike mammals, Xenopus laevis tadpoles have a high regenerative potential. To characterize this regenerative response, we performed single-cell RNA sequencing after tail amputation. By comparing naturally occurring regeneration-competent and -incompetent tadpoles, we identified a previously unrecognized cell type, which we term the regeneration-organizing cell (ROC). ROCs are present in the epidermis during normal tail development and specifically relocalize to the amputation plane of regeneration-competent tadpoles, forming the wound epidermis. Genetic ablation or manual removal of ROCs blocks regeneration, whereas transplantation of ROC-containing grafts induces ectopic outgrowths in early embryos. Transcriptional profiling revealed that ROCs secrete ligands associated with key regenerative pathways, signaling to progenitors to reconstitute lost tissue. These findings reveal the cellular mechanism through which ROCs form the wound epidermis and ensure successful regeneration.
Assuntos
Epiderme/fisiologia , Reepitelização/fisiologia , Cauda/fisiologia , Xenopus laevis/fisiologia , Animais , Células Epidérmicas/fisiologia , Reepitelização/genética , Análise de Sequência de RNA/métodos , Análise de Célula Única/métodos , Transcriptoma , Proteínas de Xenopus/genética , Xenopus laevis/genéticaRESUMO
Initial nuclear transplantation experiments in Xenopus eggs provided the first evidence for the conservation of the genome after cellular differentiation. This Discovery-in-Context Review recounts the early experiments that led to successful nuclear transfer in amphibians and the establishment of totipotency of a differentiated cell and shows how these discoveries paved the way for similar cloning experiments in other organisms.
Assuntos
Núcleo Celular/genética , Clonagem de Organismos/métodos , Genoma , Técnicas de Transferência Nuclear/tendências , Animais , Bovinos , Diferenciação Celular , Clonagem de Organismos/história , Instabilidade Genômica , História do Século XX , História do Século XXI , Camundongos , Técnicas de Transferência Nuclear/história , Oócitos/citologia , Oócitos/metabolismo , Ovinos , Suínos , Xenopus laevis/genéticaRESUMO
Recent advances in genome editing using programmable nucleases have revolutionized gene targeting in various organisms. Successful gene knock-out has been shown in Xenopus, a widely used model organism, although a system enabling less mosaic knock-out in founder embryos (F0) needs to be explored in order to judge phenotypes in the F0 generation. Here, we injected modified highly active transcription activator-like effector nuclease (TALEN) mRNA to oocytes at the germinal vesicle (GV) stage, followed by in vitro maturation and intracytoplasmic sperm injection, to achieve a full knock-out in F0 embryos. Unlike conventional injection methods to fertilized embryos, the injection of TALEN mRNA into GV oocytes allows expression of nucleases before fertilization, enabling them to work from an earlier stage. Using this procedure, most of developed embryos showed full knock-out phenotypes of the pigmentation gene tyrosinase and/or embryonic lethal gene pax6 in the founder generation. In addition, our method permitted a large 1 kb deletion. Thus, we describe nearly complete gene knock-out phenotypes in Xenopus laevis F0 embryos. The presented method will help to accelerate the production of knock-out frogs since we can bypass an extra generation of about 1 year in Xenopus laevis. Meantime, our method provides a unique opportunity to rapidly test the developmental effects of disrupting those genes that do not permit growth to an adult able to reproduce. In addition, the protocol shown here is considerably less invasive than the previously used host transfer since our protocol does not require surgery. The experimental scheme presented is potentially applicable to other organisms such as mammals and fish to resolve common issues of mosaicism in founders.
Assuntos
Desoxirribonucleases/genética , Efeito Fundador , Técnicas de Inativação de Genes/métodos , RNA Mensageiro/genética , Xenopus laevis/genética , Animais , Sequência de Bases , Desoxirribonucleases/metabolismo , Embrião não Mamífero , Proteínas do Olho/genética , Feminino , Fertilização , Regulação da Expressão Gênica no Desenvolvimento , Genes Letais , Proteínas de Homeodomínio/genética , Masculino , Microinjeções , Dados de Sequência Molecular , Monofenol Mono-Oxigenase/deficiência , Monofenol Mono-Oxigenase/genética , Oócitos/citologia , Oócitos/crescimento & desenvolvimento , Oócitos/metabolismo , Fator de Transcrição PAX6 , Fatores de Transcrição Box Pareados/deficiência , Fatores de Transcrição Box Pareados/genética , Fenótipo , RNA Mensageiro/metabolismo , Proteínas Repressoras/deficiência , Proteínas Repressoras/genética , Alinhamento de Sequência , Injeções de Esperma Intracitoplásmicas , Ativação Transcricional , Xenopus laevis/embriologiaRESUMO
POST-NOBEL PERSPECTIVE: This brief introduction is followed by a published version of my Nobel Laureate lecture, re-published herein with the kind permission of the Nobel Foundation. Much has happened since my original research, for which that prize was awarded. Hence, I am pleased to offer a few thoughts about the future of my research and its possible impact on humankind.Although the original work on nuclear transfer and reprogramming was done over half a century ago, advances continue to be made. In particular the Takahashi and Yamanaka induced pluripotent stem cells (iPS) procedure has opened up the field of cell replacement to a great extent. Now, more recently, further advances make this whole field come closer to actual usefulness for humans. Recently, in the UK, the government approved the use of mitochondrial replacement therapy to avoid the problems associated with genetically defective mitochondria in certain women. Although the House of Commons (members of Parliament) and the House of Lords had to debate and discuss whether to allow this kind of human therapy, I was very pleased to find that both bodies approved this procedure. This means that a patient can choose to make use of the procedure; it does not in any way force an individual to have a procedure that they are not comfortable with. In my view, this is a great advance in respect to giving patients a choice about the treatment they receive. I am told that the UK is the first country in the world to approve mitochondrial replacement therapy.Now that the Clustered Regularly Interspaced Short Palindromic Repeat (CRISPr) technology is being widely used and works well, one can foresee that there will be those who wish to use this technology to make genetic changes to humans. For example, if a human has a gene that makes it susceptible to infection or any other disorder, the removal of that gene might give such a person immunity from that disease. If this gene deletion is done within the germ line, the genetic change will be inherited. However, one can imagine that various people will strongly object and say that this technology should not be allowed. I would very much hope that various regulatory bodies, governments, etc. will allow the choice to remain with the individual. I can see no argument for such bodies to make a law that removes any choice whatsoever by an individual.
RESUMO
Eggs and oocytes have a remarkable ability to induce transcription of sperm after normal fertilization and in somatic nuclei after somatic cell nuclear transfer. This ability of eggs and oocytes is essential for normal development. Nuclear actin and actin-binding proteins have been shown to contribute to transcription, although their mode of action is elusive. Here, we find that Xenopus Wave1, previously characterized as a protein involved in actin cytoskeleton organization, is present in the oocyte nucleus and is required for efficient transcriptional reprogramming. Moreover, Wave1 knockdown in embryos results in abnormal development and defective hox gene activation. Nuclear Wave1 binds by its WHD domain to active transcription components, and this binding contributes to the action of RNA polymerase II. We identify Wave1 as a maternal reprogramming factor that also has a necessary role in gene activation in development.
Assuntos
Reprogramação Celular/genética , Regulação da Expressão Gênica no Desenvolvimento , Proteínas Nucleares/fisiologia , Oócitos/crescimento & desenvolvimento , Transcrição Gênica , Ativação Transcricional , Família de Proteínas da Síndrome de Wiskott-Aldrich/fisiologia , Proteínas de Xenopus/fisiologia , Xenopus laevis/embriologia , Animais , Núcleo Celular/metabolismo , Feminino , Técnicas de Silenciamento de Genes , Genes Homeobox , Camundongos , Proteínas Nucleares/genética , Oócitos/metabolismo , Estrutura Terciária de Proteína , RNA Polimerase II/metabolismo , Família de Proteínas da Síndrome de Wiskott-Aldrich/genética , Proteínas de Xenopus/genética , Xenopus laevis/genéticaRESUMO
It is relatively unusual for the Nobel Prize in Physiology or Medicine to be made, to a large extent, on the basis of a single author paper, published over 50 years ago, for work carried out by a graduate student. This was largely true of a paper published in 1962 in the journal Development (called at that time the Journal of Embryology and Experimental Morphology). The main subject of that paper was the production of normal tadpoles from the nuclei of intestinal epithelium cells of Xenopus laevis. In view of this unusual situation, I have been invited to comment on the 1962 paper.
Assuntos
Técnicas de Transferência Nuclear/história , Xenopus laevis/crescimento & desenvolvimento , Animais , Núcleo Celular/genética , Reprogramação Celular , Clonagem de Organismos/história , História do Século XX , História do Século XXI , Mucosa Intestinal/citologia , Prêmio Nobel , Óvulo , Fisiologia/história , Xenopus laevis/genéticaRESUMO
Sir John Gurdon and Professor Shinya Yamanaka were the recipients of the 2012 Nobel Prize for Physiology or Medicine. This Spotlight article is a commentary on the early nuclear transplant work in Xenopus, which was very important for the Nobel award in 2012, and the influence of this work on the reprogramming field.
Assuntos
Núcleo Celular/genética , Clonagem de Organismos/métodos , Óvulo/citologia , Animais , Diferenciação Celular , Núcleo Celular/metabolismo , Reprogramação Celular , Clonagem de Organismos/história , Embrião não Mamífero/citologia , Embrião não Mamífero/embriologia , Embrião não Mamífero/metabolismo , Desenvolvimento Embrionário , Endoderma/citologia , Endoderma/metabolismo , Epigênese Genética , História do Século XX , História do Século XXI , Camundongos , Prêmio Nobel , Técnicas de Transferência Nuclear/história , Óvulo/metabolismo , Fisiologia/história , Xenopus/embriologia , Xenopus/genéticaRESUMO
There is currently particular interest in the field of nuclear reprogramming, a process by which the identity of specialised cells may be changed, typically to an embryonic-like state. Reprogramming procedures provide insight into many mechanisms of fundamental cell biology and have several promising applications, most notably in healthcare through the development of human disease models and patient-specific tissue-replacement therapies. Here, we introduce the field of nuclear reprogramming and briefly discuss six of the procedures by which reprogramming may be experimentally performed: nuclear transfer to eggs or oocytes, cell fusion, extract treatment, direct reprogramming to pluripotency and transdifferentiation.
Assuntos
Reprogramação Celular , Técnicas de Transferência Nuclear , Óvulo/metabolismo , Animais , Membrana Celular/metabolismo , Transdiferenciação Celular , Embrião de Mamíferos/citologia , Embrião de Mamíferos/metabolismo , Epigênese Genética , Humanos , Células-Tronco Pluripotentes Induzidas/citologia , Células-Tronco Pluripotentes Induzidas/metabolismo , Prófase Meiótica I , Metáfase , Óvulo/citologia , Transcrição GênicaRESUMO
Proper regulation of transcription is essential for cells to acquire and maintain cell identity. Transcriptional activation plays a central role in gene regulation and can be modulated by introducing transcriptional activators such as transcription factors. Activators act on their specific target genes to induce transcription. Reprogramming experiments have revealed that as cells become differentiated, some genes are highly silenced and even introduction of activators that target these silenced genes does not induce transcription. This can be explained by chromatin-based repression that restricts access of transcriptional activators to silenced genes. Transcriptional activation from these genes can be accomplished by opening chromatin, in addition to providing activators. Once a de novo transcription network is established, cells are differentiated or reprogrammed to a new cell type. Emerging evidence suggests that actin in the nucleus (nuclear actin) and nuclear actin-binding proteins are implicated in these transcriptional regulatory processes. This review summarizes roles of nuclear actin and actin-binding proteins in transcriptional regulation. We also discuss possible functions of nuclear actin during reprogramming in the context of transcription and chromatin remodeling.
Assuntos
Actinas/metabolismo , Núcleo Celular/metabolismo , Reprogramação Celular , Regulação da Expressão Gênica , Proteínas dos Microfilamentos/metabolismo , Transcrição Gênica , Animais , Diferenciação Celular , Montagem e Desmontagem da Cromatina , Drosophila melanogaster , Inativação Gênica , Humanos , Camundongos , Oócitos/citologia , Ativação Transcricional , Xenopus/metabolismoRESUMO
Nuclear transfer (NT) remains the most effective method to reprogram somatic cells to totipotency. Somatic cell nuclear transfer (SCNT) efficiency however remains low, but recurrent problems occurring in partially reprogrammed cloned embryos have recently been identified and some remedied. In particular, the trophectoderm has been identified as a lineage whose reprogramming success has a large influence on SCNT embryo development. Several interspecific hybrid and cybrid reprogramming systems have been developed as they offer various technical advantages and potential applications, and together with SCNT, they have led to the identification of a series of reprogramming events and responsible reprogramming factors. Interspecific incompatibilities hinder full exploitation of cross-species reprogramming systems, yet recent findings suggest that these may not constitute insurmountable obstacles.
Assuntos
Reprogramação Celular , Desenvolvimento Embrionário/genética , Regulação da Expressão Gênica no Desenvolvimento , Técnicas de Transferência Nuclear , Animais , Núcleo Celular/genética , Núcleo Celular/metabolismo , Clonagem Molecular , Embrião de Mamíferos/citologia , Embrião de Mamíferos/metabolismo , HumanosRESUMO
Nucleocytoplasmic hybrid (cybrid) embryos result from the combination of the nucleus of one species, and the egg cytoplasm of another species. Cybrid embryos can be obtained either in the haploid state by the cross-fertilization or intra-cytoplasmic injection of an enucleated egg with sperm from another species, or in the diploid state by the technique of interspecies somatic cell nuclear transfer (iSCNT). Cybrids that originate from the combination of the nucleus and the cytoplasm of distantly related species commonly expire during early embryonic development, and the cause of this arrest is currently under investigation. Here we show that cells isolated from a Xenopus cybrid (Xenopus (Silurana) tropicalis haploid nucleus combined with Xenopus laevis egg cytoplasm) embryo are unable to proliferate and expand normally in vitro. We also provide evidence that the lack of nuclear donor species maternal poly(A)(+) RNA-dependent factors in the recipient species egg may contribute to the developmental dead-end of distantly-related cybrid embryos. Overall, the data are consistent with the view that the development promoted by one species' nucleus is dependent on the presence of maternally-derived, mRNA encoded, species-specific factors. These results also show that cybrid development can be improved without nuclear species mitochondria supplementation or replacement.
RESUMO
Patient-specific somatic cell reprogramming is likely to have a large impact on medicine by providing a source of cells for disease modelling and regenerative medicine. Several strategies can be used to reprogram cells, yet they are generally characterised by a low reprogramming efficiency, reflecting the remarkable stability of the differentiated state. Transcription factors, chromatin modifications, and noncoding RNAs can increase the efficiency of reprogramming. However, the success of nuclear reprogramming is limited by epigenetic mechanisms that stabilise the state of gene expression in somatic cells and thereby resist efficient reprogramming. We review here the factors that influence reprogramming efficiency, especially those that restrict the natural reprogramming mechanisms of eggs and oocytes. We see this as a step towards understanding the mechanisms by which nuclear reprogramming takes place.
Assuntos
Reprogramação Celular , Epigênese Genética , Animais , Divisão Celular , Metilação de DNA , Humanos , Modelos Genéticos , Transcrição GênicaRESUMO
Differentiated cells can be experimentally reprogrammed back to pluripotency by nuclear transfer, cell fusion or induced pluripotent stem cell technology. Nuclear transfer and cell fusion can lead to efficient reprogramming of gene expression. The egg and oocyte reprogramming process includes the exchange of somatic proteins for oocyte proteins, the post-translational modification of histones and the demethylation of DNA. These events occur in an ordered manner and on a defined timescale, indicating that reprogramming by nuclear transfer and by cell fusion rely on deterministic processes.
Assuntos
Núcleo Celular/metabolismo , Reprogramação Celular , Oócitos/metabolismo , Óvulo/metabolismo , Animais , Desdiferenciação Celular , Fusão Celular , Núcleo Celular/genética , Cromatina/genética , Cromatina/metabolismo , Feminino , Expressão Gênica , Histonas/metabolismo , Humanos , Células-Tronco Pluripotentes Induzidas/citologia , Células-Tronco Pluripotentes Induzidas/metabolismo , Metilação , Técnicas de Transferência Nuclear , Oócitos/citologia , Óvulo/citologia , Fatores de Tempo , Xenopus laevisRESUMO
We review experiments in which somatic cell nuclei are transplanted singly to enucleated eggs (metaphase II) in amphibia and mammals and as multiple nuclei to the germinal vesicle of amphibian oocytes (prophase I). These experiments have shown the totipotency of some somatic cell nuclei, as well as switches in cell type and changes in gene expression. Abnormalities of nuclear transplant embryo development increase greatly as nuclei are taken from progressively more differentiated donor cells. The molecular changes that accompany the reprogramming of transplanted nuclei help to indicate the mechanisms used by eggs and oocytes to reprogram gene expression. We discuss the importance of chromosomal protein exchange, of transcription factor supply, and of chromatin access in reprogramming.