Herbal-Based Green Synthesis of TB-ZnO-TiO(II) Nanoparticles Composite From Terminalia bellirica: Characterization, Toxicity Assay, Antioxidant Assay, and Antimicrobial Activity.

Background Terminalia bellirica leaf extract was used as an herbal to get an aqueous extract of Tb-ZnO-TiO2 (zinc and titanium dioxide) nanoparticles composite, and this was subsequently subjected to an analysis of its antioxidant properties and possible antimicrobial activity against gram-negative and gram-positive bacteria. Employing the 2,2-Diphenyl-1-picrylhydrazyl and hydrogen peroxide assay techniques for antioxidant properties. In addition to their biocompatibility, rapid biodegradability, and low toxicity, herbal-based nanoparticles (Tb-ZnO-TiO2 NPs composite) synthesized by T. bellirica have drawn a lot of interest as promising options for administering drugs and effective antimicrobial applications. Materials and methods The form and dimensions of the dispersion of the synthesized nanoparticles were investigated through scanning electron microscopy (SEM), Fourier Transform Infrared Spectroscopy, and UV-visible for particle characterization. Nanoparticles were analyzed for antimicrobial activity using the well diffusion method. Ascorbic acid and vitamin E were used as two separate controls for antioxidant assay with different concentrations, and also toxicity assay was done by using zebrafish embryos. Results Tb-ZnO-TiO2 NPs composite were obtained as a powder, the X-beam diffraction (XRD) result revealed a small quantity of impurities and revealed that the structure was spherical in nature. A unique absorption peak for Tb-ZnO-TiO2 NPs composite may be seen in UV-Vis spectroscopy which is in the region of 260 to 320 nm. The Tb-ZnO-TiO2 NPs composite antibacterial efficacy was evaluated and showed noted antibacterial activity and free radical scavenging activity with less toxicity. Conclusion The results demonstrated the Tb-ZnO-TiO2 NPs composite has strong antioxidant qualities and enormous antibacterial activity obtained from T. bellirica extract. Therefore, the Tb-ZnO-TiO2 NPs composite synthesized nanoparticles can be used in biomedical applications as an effective antioxidant and antibacterial reagent.

Serial quantification of CRP and total leukocyte count as a complementary tool in neonatal sepsis.

Early detection and appropriate treatment of newborn sepsis reduce mortality and morbidity. A rapid, inexpensive laboratory approach is needed to assess newborn sepsis, even though blood culture is the gold standard for diagnosis. To compare serial CRP and Total Leukocyte Count (WBC) with blood culture, this study aimed to evaluate the role of newborn sepsis. A total 148 neonates with clinical symptoms of sepsis were included .CRP was measured by quantitative immuno turbidimetric method andotal leukocyte count (WBC) was measured by automated cell counter. CRP1 and WBC1 were measured within 6 hours of clinical symptoms. CRP2 and WBC2 were measured after 48 hours of clinical symptoms. Sensitivity, specificity, PPV, NPV of CRP1 and CRP2,WBC 1and WBC 2 were compared with culture positive and negative sepsis.CRP 2 showed high sensitivity 96% and high NPV95% with significant p value <0.0001. WBC2 has high sensitivity (90.57%) and NPV (91%) with significant p value <0.0001. CRP 1 has sensitivity 83%and NPV 82.3%, with p value < 0.001.WBC1 has lowest sensitivity (62.2%) and NPV (71.4%) compared to all other parameters. Serial CRP and WBC measurements are useful in the diagnosis of neonatal sepsis. Measurement of CRP and Total Leukocyte Count (WBC) after 48 hours of clinical symptoms were considered promptly for diagnose neonatal sepsis.

Linking chronic periodontitis and oral cancer: A review.

The aim of this article is to survey the accessible writing on the pathogenetic systems engaged with the relationship between oral malignancy and periodontitis. Gingival tissue contains multiple microbiota, which can induce inflammatory reactions. This reaction plays a crucial role in assessing the susceptibility of patients to periodontal diseases. The link between chronic periodontitis and the risk of malignancy through this inflammation of the affected epithelium have been studied thoroughly. Many studies have reported that, chronic periodontitis has systemic influence which has high risk of developing different types of cancers. Also, various confounding factors such as consumption of alcohol, smoking, diet, age and gender have been found to be associated with both chronic periodontitis and oral cancer. An online quest for a wide range of articles distributed was started utilizing MEDLINE/PubMed, with the keywords, for example, 'oral squamous cell carcinoma (OSCC)', 'oral microbiota,' 'microorganisms and malignancy and Porphyromonas gingivalis. This review aimed to study the current literature linking chronic periodontitis and oral cancer.

Esthetic lip repositioning: A cosmetic approach for correction of gummy smile - A case series.

Gummy smile has been a prevalent esthetic disorder commonly affecting younger individuals due to various causes such as skeletal, dento-alveolar, or soft-tissue origin. It can be due to jaw deformities, altered passive eruption, or tooth malpositioning. Usually, the corrective measures incorporate orthognathic surgery and orthodontic treatment. Orthognathic surgery results in significant morbidity and hospitalization. Lip repositioning can be employed as an alternative cosmetic treatment modality for the correction of an excessive gingival display with minimal risk or side effects. Although there are many articles existing in the literature on this topic, a series of three cases is unique in demonstrating the esthetic smile enhancement in an orthodontic patient and also a combination of lip-repositioning technique and laser-assisted crown lengthening in vertical maxillary excess cases. This case series also showed the predictability of lip-repositioning technique with a follow-up of 2 years.