Rehabilitación protésica en odontopediatría y sus efectos en la modificación conductual de un niño / Prosthetic rehabilitation in pediatric dentistry and its effects on the behavioral modification of a child

La caries de la temprana infancia puede afectar severamente la dentición primaria que requiere tratamientos complejos y en ocasiones la extracción del diente afectado. Se presenta informe de caso sobre la rehabilitación dental integral de un paciente de 6 años, la evolución de su comportamiento y la mejora en su calidad de vida, autoestima e interacción social. El plan de tratamiento incluyó una fase de adaptación a la consulta odontológica, eliminación de caries, restauración dental, exodoncias y elaboración de dos prótesis removibles. Al inicio del tratamiento presentó temor y rechazo al tratamiento, pero con el avance del proceso rehabilitador odontológico y la evidencia de los resultados, el comportamiento del niño fue de aceptación y colaboración. Su evolución positiva de comportamiento se presentó también en sus relaciones sociales, aumentó la interacción con otras personas y su participación en juegos con otros niños. La rehabilitación protésica contribuyó a recuperar las funciones de masticación y fonación. La renovación de la estética dental produjo un significativo cambio de conducta, mejora en su autoestima con un impacto psicológico positivo en su calidad de vida

Early childhood caries can severely affect the primary dentition requiring complex treatments and sometimes the extraction of the affected tooth. A case report is presented on the comprehensive dental rehabilitation of a 6-year-old patient, the evolution of his behavior and the improvement in his quality of life, self-esteem and social interaction. The treatment plan included a phase of adaptation to the dental consultation, elimination of cavities, dental restoration, extractions and elaboration of two removable prostheses. At the beginning of the treatment, he presented fear and rejection of the treatment, but with the progress of the dental rehabilitation process and the evidence of the results, the child's behavior was one of acceptance and collaboration. Their positive behavior evolution was also present in their social relationships, increased interaction with other people and their participation in games with other children. Prosthetic rehabilitation contributed to recovering the functions of chewing and phonation. The renovation of dental aesthetics produced a significant change in behavior, an improvement in their self-esteem with a positive psychological impact on their quality of life

Enhancement of alkaline-oxidative delignification of wheat straw by semi-batch operation in a stirred tank reactor.

This paper compares a semi-batch operation and a conventional one of an alkaline oxidative pretreatment of wheat straw carried out in a stirred tank reactor. For the pretreatment, different concentrations of biomass (6% up to 12% w/v) and two different particle sizes (mesh #40-60 and #>60) were experimented. The performance of processes was evaluated through the analysis of lignocellulosic composition of the biomass, and the enzymatic hydrolysis of pretreated biomass using the Cellic® CTec2 enzyme complex by Novozymes®. The process time of semi-batch operation is significantly lower than the batch one and enables a higher load of biomass, showing a delignification yield between 55 and 60%. In the first 5 h of reaction time, the enzymatic hydrolysis experiments reached their maximum yields of 72 and 66% according to reducing sugars conversion when using the mesh #>60 mesh and #40-60, respectively.

Purification and characterization of an extracellular ß-glucosidase from Sporothrix schenckii.

An extracellular ß-glucosidase (E.C. 3.2.1.21), induced by cellulose in the mycelial form of human pathogen fungus Sporothrix schenckii, was purified to homogeneity using hydroxyapatite (HAp) adsorption chromatography in batch and Sephacryl S200-HR size exclusion chromatography. The molecular mass of the purified enzyme was estimated to be 197 kDa by size exclusion chromatography with a subunit of 96.8 kDa determined by SDS/PAGE. The ß-glucosidase exhibited optimum catalytic activity at pH 5.5/45 °C and was relatively stable for up to 24 h at 45 °C. Isoelectric focusing displayed an enzyme with a pI value of 4.0. Its activity was inhibited by Fe2+ but not by any other ions or chelating agents. Km and Vmax values of the purified enzyme were 0.012 mm and 2.56 nmol·min-1·mg-1, respectively, using 4-methylumbelliferyl ß-D-glucopyranoside (4-MUG) as the substrate and 44.14 mm and 22.49 nmol·min-1·mg-1 when p-nitrophenyl ß-D-glucopyranoside (p-NPG) was used. The purified ß-glucosidase was active against cellobioside, laminarin, 4-MUG, and p-NPG and slightly active against 4-methylumbelliferyl ß-D-cellobioside and p-nitrophenyl ß-D-cellobioside but did not hydrolyze 4-methylumbelliferyl ß-D-xyloside, 4-methylumbelliferyl ß-D-galactopyranoside nor 4-methylumbelliferyl α-D-glucopyranoside. In addition, the enzyme showed transglycosylation activity when it was incubated along with different oligosaccharides. Whether the transglycosylation and cellulase activities function in vivo as a mechanism involved in the degradation of cellulolytic biomass in the saprophytic stage of S. schenckii remains to be determined.

Race and ethnicity of mothers with Spanish surnames.

The objective of this study was to assess how hospital designation of race/ethnicity based on Spanish surnames of mothers correlated with the self-report, and explores how these mothers identified their own race/ethnicity using the National Institutes of Health (NIH) categories. 235 mothers were enrolled prospectively and asked to report their race/ethnicity. Positive predictive value (PPV) of using surnames as a tool for assigning race/ethnicity was determined. The PPV of using surnames to identify ethnicity and race was 79 and 30%, respectively. 57% of mothers could not identify their race by the NIH categories. Although Spanish surnames more accurately reflected ethnicity than race, its use was associated with substantial discrepancies.

Flow injection and HPLC determination of furosemide using pulsed amperometric detection at microelectrodes.

The flow-injection and HPLC determination of the diuretic drug furosemide using pulsed amperometric detection (PAD) at cylindrical carbon fibre microelectrodes (CFMEs) is reported. Experimental conditions such as pH (6.5) and buffer concentration (0.05 mol l(-1) HPO4(2-)/H2PO4(-)) were optimized using square-wave voltammetry (SWV). Repetitive flow-injection amperometric measurements at +1.25 V for furosemide showed a continuous decrease in the peak current, probably as a consequence of the microelectrode surface fouling. However, a suitable amperometric detection of furosemide was achieved using a PAD program consisting of a two-step potential waveform with alternating anodic and cathodic polarization. The anodic (detection) potential was +1.25 V (time of application 0.1 s), and the cathodic (cleaning) potential was -0.20 V (t=0.2 s). A linear calibration graph was obtained for furosemide in the 5.0 x 10(-7)-1.0 x 10(-4) mol l(-1) concentration range, with a limit of detection of 1.7 x 10(-7) mol l(-1). HPLC-PAD at carbon fibre microelectrodes was used for the determination of furosemide in the presence of several thiouracil drugs and oxytetracycline (OTC). The mobile phase selected was a 25:75 acetonitrile:5.0 x 10(-3) mol l(-1) NaH2PO4 (pH 5.0) mixture. A linear calibration graph was obtained for furosemide in the 1-100 microM range, with a limit of detection of 0.55 microM. The usefulness of this method for the determination of furosemide in real samples was evaluated by performing the analysis of commercial milk samples spiked with furosemide at a concentration level of 4.5 x 10(-7) mol l(-1) (150 ng ml(-1)), as well as with other thiouracil drugs and OTC. A mean recovery of 95+/-5% furosemide was obtained.

Characterisation of horseradish peroxidase immobilisation on an electrochemical biosensor by colorimetric and amperometric techniques.

This study presents the use of complementary colorimetric and amperometric techniques to measure the quantity of protein or enzyme immobilised onto a carbon paste electrode modified with a layer of electrodeposited polyaniline. By applying a solution of bovine serum albumin at 0.75 mg/ml, efficient blocking of the electrode from electroactive species in the bulk solution could be achieved. When the horseradish peroxidase was immobilised on the electrode, optimal amperometric responses from hydrogen peroxide reduction were achieved at approximately the same concentration. The mass of enzyme immobilised at this solution concentration was determined by a colorimetric enzyme assay to be equivalent to the formation of a protein monolayer. Under these conditions, amperometric responses from the immobilised layer are maximised and non-specific bulk solution interactions are minimised. At higher immobilised protein concentrations, diminished amperometric responses may be due to inhibited diffusion of hydrogen peroxide to enzyme which is in electronic communication with the electrode surface, or impeded electron transfer.

Carbon fiber cylindrical microelectrode-based detector for the determination of antithyroid drugs.

An amperometric flow-injection method for the determination of antithyroid drugs such as 6-methyl-2-thiouracil (MTU) using a carbon fiber cylindrical microelectrode-based detector is reported. A home-made flow-cell specially adapted for working with cylindrical microelectrodes ranging between 4 and 10 mm in length was used for this purpose. Methanol containing 0.05 mol l(-1) tetrabutylammonium perchlorate as the supporting electrolyte was employed, and a potential of +1.6 V was selected for the amperometric detection of MTU. No cleaning or electrode surface regeneration of the fiber was necessary during the whole working day. A limit of detection of 2.6x10(-7) mol l(-1) (37 mug MTU l(-1)) was achieved. The method was applied to the determination of MTU in spiked feed samples (peas and corn flour), at the 142 mug g(-1) level. The procedure involved the extraction of the drug in methanol, a clean-up step using Florisil cartridges and the use of the standard additions method. Recoveries of 81+/-6 and 85+/-8% were obtained for peas and corn flour, respectively.