RESUMO
We have designed and built a perfusion system and perfusion chamber to allow investigation of the effects of anaesthetic agents on human cilia in vitro. Using this system, samples of human respiratory cilia can be maintained in a stable and controlled environment for several hours. We measured cilia beat frequency of nasal respiratory epithelium from 10 healthy volunteers; cilia beat frequency was constant over a 4-h period, and measurements were found to be in good agreement with previously published work [1]. In a separate study we investigated the effect of a sleep dose of propofol on cilia beat frequency in samples from six patients undergoing minor surgery; samples were obtained before and immediately after induction of anaesthesia with propofol 2-3 mg kg-1. There was no statistically significant difference in cilia beat frequency between data obtained before and after induction with propofol.
Assuntos
Anestésicos Intravenosos/farmacologia , Cílios/efeitos dos fármacos , Perfusão/instrumentação , Propofol/farmacologia , Adulto , Sobrevivência Celular , Cílios/fisiologia , Células Epiteliais , Humanos , Técnicas In Vitro , Depuração Mucociliar , Nariz/citologia , Conchas Nasais/citologiaRESUMO
The effect of halothane on human ciliated nasal epithelium was studied in vitro. Samples from 24 healthy adult volunteers were exposed to halothane in varying concentrations and cilia beat frequency was measured using the transmitted light technique. Mean cilia beat frequency was measured at 30-min intervals. There was a significant decrease in cilia beat frequency at 2 h in samples that were exposed to halothane (mean 8.4 (SD 2.5) Hz, 9.18 (2.6) Hz and 6.99 (4.9) Hz) compared with air (10.8 (2.7) Hz, 11.6 (2.1) Hz and 12.1 (2.3) Hz) (P < 0.01). The coefficient of variation of cilia beat frequency measurements increased after exposure to halothane. There was no change in the cilia beat frequency of controls exposed to air over a 3-h period.
Assuntos
Halotano/farmacologia , Depuração Mucociliar/efeitos dos fármacos , Cavidade Nasal/efeitos dos fármacos , Adulto , Cílios/efeitos dos fármacos , Cílios/fisiologia , Técnicas de Cultura , Relação Dose-Resposta a Droga , Epitélio/efeitos dos fármacos , Humanos , Microscopia de Contraste de Fase , Processamento de Sinais Assistido por Computador , Fatores de TempoRESUMO
The effect of a commonly used anaesthetic agent, halothane, on neonatal cilial function was determined. A nasal cilial sample was taken from 13 healthy, term, newborn infants. For 1 h, half of each sample was exposed to halothane and half to air. Cilial function was assessed by measuring beat frequency with a modified light transmission method. Cilial beat frequency was significantly slower in the cilia exposed to halothane, 9(s.d. 2.5)Hz, than to air, 12.9(s.d. 1.5)Hz. Decreased mucociliary clearance following halothane anaesthesia is due, at least in part, to a directly depressant effect of halothane on ciliated cells.