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J Invest Dermatol ; 135(11): 2603-2610, 2015 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-26121212

RESUMO

Many biochemical pathways involved in hair and skin development have not been investigated. Here, we reported on the lesions and investigated the mechanism underlying hair and skin abnormalities in Zdhhc13(skc4) mice with a deficiency in DHHC13, a palmitoyl-acyl transferase encoded by Zdhhc13. Homozygous affected mice showed ragged and dilapidated cuticle of the hair shaft (CUH, a hair anchoring structure), poor hair anchoring ability, and premature hair loss at early telogen phase of the hair cycle, resulting in cyclic alopecia. Furthermore, the homozygous affected mice exhibited hyperproliferation of the epidermis, disturbed cornification, fragile cornified envelope (CE, a skin barrier structure), and impaired skin barrier function. Biochemical investigations revealed that cornifelin, which contains five palmitoylation sites at cysteine residues (C58, C59, C60, C95, and C101), was a specific substrate of DHHC13 and that it was absent in the CUH and CE structures of the affected mice. Furthermore, cornifelin levels were markedly reduced when two palmitoylated cysteines were replaced with serine (C95S and C101S). Taken together, our results suggest that DHHC13 is important for hair anchoring and skin barrier function and that cornifelin deficiency contributes to cyclic alopecia and skin abnormalities in Zdhhc13(skc4) mice.


Assuntos
Aciltransferases/genética , Alopecia/genética , Anormalidades da Pele/genética , Aciltransferases/deficiência , Aciltransferases/metabolismo , Alopecia/patologia , Animais , Animais Recém-Nascidos , Western Blotting , Regulação da Expressão Gênica , Cabelo/crescimento & desenvolvimento , Imuno-Histoquímica , Proteínas de Membrana/metabolismo , Metilação , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Precursores de Proteínas/metabolismo , Sensibilidade e Especificidade , Anormalidades da Pele/patologia , Absorção Cutânea/genética
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