Monitored dressing evaluation.

The ability to measure changes in self care status for patients with cardiovascular disease is difficult to quantify since their independence is usually not limited by the need for physical assistance. Instead, limitations may include changes in vital signs that indicate excessive workload for the cardiovascular system, abnormally slow work pace, or inability to perform other tasks due to fatigue after self care is completed. Typical parameters that have been used to measure improvement in work tolerance, such as the ability to work for longer periods of time at progressively higher workloads, do not apply to self care. The purpose of this paper is to describe a monitored dressing evaluation that includes a scale to grade functional work tolerance. The scoring system provides a more objective way than usually available to measure improvements in tolerance for dressing. It is especially useful for the patient at a low level of functioning.

Structure of lactate dehydrogenase inhibitor generated from coenzyme.

Two inhibitors of lactate dehydrogenase generated during NADH storage have been isolated by chromatography. One is a dimer of the dinucleotide where the AMP moiety is unmodified. The other is also generated from NAD+ in the presence of a high concentration of phosphate ions at alkaline pH. This inhibitor was proved to be the addition compound of one phosphate group to position C-4 of the nicotinamide ring of NAD+ by NMR spectroscopy, enzymatic cleavage, and dissociation to NAD+ at neutral pH. This compound is a competitive inhibitor with respect to NAD+ in the presence of the lactate dehydrogenase with a Ki of 2 X 10(-7) M. The interaction of this inhibitor with lactate dehydrogenase is discussed relative to the structure of this enzyme.

Molar absorptivities of beta-NADH and beta-NAD at 260 nm.

To determine the molar absorptivities of beta-NADH and beta-NAD at 260 nm, we purified the reduced form of the coenzyme by repeated anion-exchange chromatography. A NADH preparation was so obtained for which the 260 nm/340 nm absorbance ratio was 2.265. When calculated with epsilon 340 beta-NADH = 6.22 times 10-3 for beta-NADH at 260 nm and 25 degrees C, a molar absorptivity of 14.1 times 10-3 liter - mol minus 1 - cm minus 1 resulted from this quotient. By use of the lactate dehydrogenase or glycerol-3-phosphate dehydrogenase assay, respectively, and referring to the new absorption coefficient for NADH at 260 nm, the molar absorptivity of beta-NAD at 260 nm and 25 degrees C was established to be 17.4 times 10-3 liter - mol minus 1 - cm minus 1. The values observed are lower than those reported thus far.