Parasitic Helminths in Snakes from The Global Legal Trade.

In recent years, the demand for snakes imported from different countries around the world has increased in Slovakia. However, such snakes can be infected with a wide variety of parasites. We have been focused on monitoring the prevalence of parasitic helminths of snakes imported to Slovakia. From 2015 to 2020, 205 samples were collected. We examined faecal samples of 185 live snakes, and a parasitological autopsy was performed on 20 carcasses. Out of a total of 205 snakes, parasitic helminths were found in 44 individuals (21.46 %). Coprological examination of live snakes confirmed positivity for the presence of helminths in 38 snakes (20.54 %). Through parasitological autopsy of all the dead snakes, we found helminths or their eggs in 6 carcasses (30.00 %). The genera of helminths found in the positive snakes were: Ophiotaenia spp., Kapsulotaenia spp., Strongyloides spp., Rhabdias spp., Ophidascaris spp., Ascaridia spp., f. Heterakidae, Kalicephalus spp., Capillaria spp., order Oxyurida and stages of the plerocercoid of an unidentifi ed species of tapeworm in the subcutaneous tissue.

The Fatal Case of an Autochthonous Heartworm Disease in a Dog from a Non-endemic Region of South-eastern Slovakia.

During the past few years, several localities with increasing Dirofilaria immitis occurrences have been identified in Slovakia; particularly in areas regarded as endemic for Dirofilaria repens up until now. In terms of that, dogs with clinically manifested heartworm disease have been referred to the veterinary ambulances more frequently. We report in this study, two autochthonous cases of D. immitisinfections diagnosed in two seven-year-old siblings of Tibetan Mastiff dogs from the Kosice region of south-eastern Slovakia. The course of the disease in both dogs were very different. The female dog did not manifest any unusual findings, however the male dog exhibited severe clinical signs of heartworm disease that lead to his death. The subsequent autopsy revealed adult D. immitis worms in the right heart ventricle and pulmonary arteries.

Significantly higher occurrence of Cryptosporidium infection in Roma children compared with non-Roma children in Slovakia.

Cryptosporidiosis is considered to be a widespread world zoonosis. The occurrence of Cryptosporidium species was investigated in Roma children in a district of Eastern Slovakia and, at the same time, also in children of non-Roma parents. In total, 103 children (54 boys and 49 girls) between 0 and 14 years of age were involved in this study. Fifty-three were Roma children and 50 children represented a non-Roma control group. Fecal samples were examined: immunologically [enzyme-linked immunosorbent assay (ELISA) test to prove antigen in the feces] and by molecular analysis [nested polymerase chain reaction (PCR)]. After the sequencing of the PCR, the products were identified as species of Cryptosporidium muris. Based on the results, the relative risk (RR) of the Cryptosporidium infection occurrence was calculated and we came to the conclusion that the risk of Cryptosporidium infection was almost 12 times higher in the Roma children compared to the non-Roma children.

Chlamydophila felis in cats--are the stray cats dangerous source of infection?

Chlamydophila felis is a causative agent of acute or chronic conjunctivitis, and pneumonia in cats. Natural transmission mostly occurs consequently to close contact with other infected cats, their aerosol and fomites. We have examined 93 cats with symptoms of acute or chronic conjunctivitis, from Kosice region in Slovakia, during the period of 2 years. Conjunctival samples were obtained from 55 domestic cats (59.14%) and 38 stray cats (40.86%). Of the total number of 93 examined animals, 42 cats were positive, which represents 45.16% overall positivity. Out of the 42 positive cats, 25 cats were stray and 17 positive cats were classified as domestic, which means that of 38 stray cats, 25 were positive (which represented 65.78% positivity) and of 55 domestic cats, 17 were positive (positivity was 30.90%). Our results showed that cats, especially stray cats, could be a dangerous source of chlamydiosis for humans.

[Identification of Ca(2+)-activated Mg(2+)-dependent ATP-hydrolase activity of membrane microsomal fraction of loach embryo (Misgurnus fossilis L.)].

The functional confirmation of availability of Ca2+ transport initially-active systems in the embryo cells of loach Misgurnus fossilis L. has been obtained. Using thapsigargin, the specific inhibitor of endoplasmic reticulum of Ca2+, Mg(2+)-ATPase, this enzyme activity was divided into thapsigargin-sensitive (actually endoplasmic reticulum Ca2+, Mg(2+)-ATPase) and thapsigargin-insensitive (plasma membrane Ca2+, Mg(2+)-ATPase) constituents. The Ca(2+)-independent Mg(2+)-dependent ATPase activity makes above 39.7% of the common Ca2+, Mg(2+)-ATPase activity of embryo loach. The periodic changes of Ca2+, Mg(2+)-ATPase activity (except for the changes of plasma membrane Ca2+, Mg(2+)-ATPase activity) were found out, which coincide with periodic [Ca2+]i oscillations during the synchronous divisions of loach blastomers embryos.

The first finding of antibodies to Encephalitozoon cuniculi in cows in Slovakia.

The presence of antibodies against Encephalitozoon cuniculi in cows was observed by the method of indirect immunofluorescence (IFAT). The animals reacting at the titre 1:64 and more were considered positive. Of the total number of 55 sera examined, 20 were positive at the titre 1:64, from which four were positive at the titre 1:256 also. Our results indicate that it is very important to carry out screening examinations in large-scale breeding of cattle due to the persistent high percentage of bovine abortions and disorders in the reproductive cycle of unknown etiology.