RESUMO
An outbreak of possible acute poisoning by Sinapis arvensis occurred in a flock of 50 fat-tailed sheep located in the Semnan province of Iran. Sinapis arvensis is an annual or winter annual plant of the genus Sinapis in the family Brassicaceae, commonly known as field mustard, wild mustard or charlock. The poisonous constituents are volatile oil of mustard, the alkaloid sinapin, and the alkaloidal glucoside sinalbin. The flock was grazing in land containing high amounts of wild mustard (Sinapis arvensis) in late spring. Seven sheep (aged between 1 and 5 years) died within approximately 3 days. The affected animals displayed signs of depression, reluctance to move, tachycardia, tachypnea, mucoid and hemorrhagic nasal discharges, pale conjunctiva, ataxia, abdominal pain, bruxism, and anorexia. Rectal temperature in these animals was normal to high (39-41.5 °C). Ruminal movements were reduced (1-2/min). Serum biochemical levels in affected sheep showed marked increases of blood urea nitrogen (BUN), Gamma glutamyl transferase (GGT), magnesium (Mg), and phosphorous (P) and a marked decrease in calcium (Ca). In urinalysis, marked hemoglobinuria and proteinuria were observed. Necropsy findings included congestion in lungs and hemorrhage on the epicardial and endocardial heart surfaces, on the surface and medulla of the kidneys, and abomasal mucosa. The liver was also congested with a nutmeg pattern. Rumen contents included digested materials and large quantities of seeds and stems of wild mustard. For the first time, our findings confirmed wild mustard toxicosis in sheep in Iran.
Assuntos
Laboratórios , Sinapis , Animais , Irã (Geográfico) , OvinosRESUMO
Vitamin D has been shown to play physiological functions beyond calcium and phosphorus homeostasis and control bone metabolism in the body since its cellular receptors are present in numerous tissues. A total of 20 healthy bulls were divided into four groups to evaluate the effect of different doses of vitamin D3 on the number of bovine blood cells. Groups A, B, C, and D received 11,000, 22,000, 33,000, and 44,000 units/kg of vitamin D3, respectively. The control group was injected with 10 ml of physiological saline intramuscularly. Blood samples were taken before the injection, as well as 2, 4, and 6 days after the injection; furthermore, the white blood cell counts (including granulocytes and lymphocytes), hematocrit, haemoglobin, and platelets were examined by a cell counter. The results showed that vitamin D could cause leukopenia (e.g., neutropenia and lymphopenia), thrombocytopenia, as well as an increase in hematocrit and hemoglobin levels in the blood. Although the mentioned increase or decrease is largely dose- and time-dependent, the first and best group to indicate this is group B. To find the second group, the investigation of the long-term effects of injections, especially in high doses, and evaluation of different tests are required with larger groups over a longer period.
Assuntos
Colecalciferol , Vitamina D , Animais , Cálcio/metabolismo , Bovinos , Colecalciferol/farmacologia , Suplementos Nutricionais , Masculino , Vitamina D/metabolismo , Vitamina D/farmacologiaRESUMO
PURPOSE: To report our 14-year experience with orbital exenteration and assess risk factors for poor prognosis by focusing on conjunctival melanoma. PATIENTS AND METHOD: A retrospective study was conducted in our tertiary care centre (Jules Gonin Eye Hospital, Lausanne, Switzerland) between 2003 and 2017. Inclusion criteria were patients aged ≥18 years with a follow-up >12 months, without metastatic spread at the time of surgery. Data recorded were age, gender, tumour histology, surgical technique, postoperative complications, surgical margin status, local recurrence, postoperative radiation beam therapy and metastatic status. RESULTS: Twenty-five patients with a mean age of 63.2 years (38-92) were included. Conjunctival melanoma was the most frequently identified tumour (n = 14, 56%) followed by conjunctival squamous cell carcinoma (n = 4, 16%), sebaceous carcinoma (n = 3, 12%), choroidal melanoma (n = 2, 8%) and basal cell carcinoma (n = 2, 8%). Eighteen tumours (72%) originated from the conjunctival tissue. Clear surgical margins were achieved in 21 (84%) patients. Fourteen (56%) patients experienced distant metastases and died from metastatic spread after a mean follow-up of 52.3 months (6-120). The 1-, 3- and 5-year overall survival (OS) was 96%, 72% and 60%, respectively. In the univariate analysis, positive surgical margins, local recurrence and metachronous metastases were associated with a decreased OS (p = 0.002, p = 0.005 and p = 0.007, respectively). In the multivariate analysis, positive surgical margins and metachronous metastases were also associated with a decreased OS (p = 0.02 and p = 0.042, respectively). Conjunctival melanoma was not associated with a poorer prognosis (p = 0.280). CONCLUSION: Free surgical margins are needed to increase OS. To achieve clearer surgical margins, neoadjuvant targeted therapies/immunotherapies may be considered.
Assuntos
Neoplasias da Túnica Conjuntiva , Melanoma , Adulto , Idoso , Idoso de 80 Anos ou mais , Neoplasias da Túnica Conjuntiva/cirurgia , Hospitais , Humanos , Melanoma/cirurgia , Pessoa de Meia-Idade , Recidiva Local de Neoplasia/epidemiologia , Exenteração Orbitária , Estudos RetrospectivosRESUMO
PURPOSE: To assess the efficacy and safety of bone-anchored dental implant placement at the same time as orbital exenteration compared with delayed implant placement. MATERIALS AND METHODS: A retrospective comparative study was conducted in a single tertiary care center between December 2003 and December 2017. Patients who underwent bone-anchored implant placement at the same time as orbital exenteration were included (group 1) and compared with patients who underwent delayed implant placement (group 2). The main outcome was the 1-year success rate of implant osseointegration. The secondary outcomes were the 5-year success rate of osseointegration, postoperative complications, and time between orbital exenteration and prosthesis placement. RESULTS: Ten and 11 patients (21 and 22 implants) with a mean follow-up of 50.2 and 48.5 months were included in groups 1 and 2, respectively. Patients in group 1 were significantly older (69.7 vs 61.2 years, P = .026). No significant differences were found between both groups regarding tumor type and location, prior treatments, smoking status, and postoperative radiation beam radiotherapy. The 1- and 5-year success rates of osseointegration were 95.5% and 93.3% in group 1, and 100% and 100% in group 2, respectively (P = .488 and P = .450 between both groups). One implant did not osseointegrate in group 1 due to osteitis. Ethmoidal fistula was the most common postoperative complication found in both groups (P = .670). The mean time between orbital exenteration and episthesis placement was 8 (3 to 14) vs 11 (3 to 15) months in groups 1 and 2, respectively (P = .467). CONCLUSION: Placing implants at the same time as orbital exenteration is a viable procedure. It reduces surgical morbidity and allows placement of implants in a nonirradiated area.
Assuntos
Implantes Dentários , Osseointegração , Implantação de Prótese , Seguimentos , Humanos , Duração da Cirurgia , Estudos Retrospectivos , Resultado do TratamentoRESUMO
INTRODUCTION: Orbital exenteration is a radical anatomically and psychologically disfiguring procedure. It is mostly performed for management of orbital cancers or cancers with orbital involvement. The lack of benefit in terms of overall survival and the development of new molecular therapies (targeted therapies, immunotherapy) in recent years leads us to question its use. The goal of our review is to answer to the following question: is orbital exenteration a viable procedure in 2019? MATERIALS AND METHODS: A literature review was performed using the PUBMED and MEDLINE databases. The following terms were used then crossed with each other: "orbital exenteration", "exenterated socket", "overall survival", "life expectancy", "orbital reconstruction", "socket reconstruction". Oncology articles from the past 15 years were included and separated into those in the oculoplastic literature and those in the ENT literature. RESULTS: Nineteen articles were included in this review. Eyelid tumours represent the main etiology of orbital exenteration. Basal cell carcinoma is the most frequently incriminated tumor, while sebaceous carcinoma and conjunctival squamous cell carcinoma are the most frequently encountered in Asian series. Non-conservative orbital exenteration is the most prevalent surgery performed. Orbital reconstruction depends on the surgeon's speciality: healing by secondary intention and split thickness skin grafts are mostly performed by oculoplastic surgeons, whereas regional or free flaps are mostly performed by ENT surgeons. Cerebrospinal fluid leakage is the most common intraoperative complication, encountered in 0 to 13 % of cases. The most common postoperative complications are ethmoid fistula and infection of the operative site, encountered in 0 to 50 % and 0 to 43 % of cases respectively. Orbital exenteration allows surgical resection of R0 tumors in 42.5 % to 97 % of cases. Overall survival following orbital exenteration is 83 % (50.5-97) and 65 % (37-92) at 1 and 5 years respectively. Identified risk factors for poor overall survival are: age, tumor histology (worse prognosis with choroidal melanoma, better prognosis with basal cell carcinoma), non-R0 surgical resection, locally advanced tumors (size>20mm, BCVA<20/400 and the presence of metastases at diagnosis). Recent studies have demonstrated favorable outcomes when managing locally advanced basal cell carcinoma, lacrimal gland cancer and conjunctival melanoma with targeted therapies or immunotherapies without performing orbital exenteration. CONCLUSION: Orbital exenteration remains a major part of our therapeutic arsenal. Although orbital exenteration has failed to demonstrate any overall survival benefit, it allows satisfactory local control of the disease with an increasingly less invasive procedure. The development of targeted therapies and immunotherapies may change our therapeutic decisions in the future.
Assuntos
Exenteração Orbitária , Neoplasias Oculares/diagnóstico , Neoplasias Oculares/epidemiologia , Neoplasias Oculares/cirurgia , Neoplasias Palpebrais/diagnóstico , Neoplasias Palpebrais/epidemiologia , Neoplasias Palpebrais/cirurgia , História do Século XXI , Humanos , Expectativa de Vida/tendências , Exenteração Orbitária/história , Exenteração Orbitária/mortalidade , Exenteração Orbitária/tendências , Neoplasias Orbitárias/diagnóstico , Neoplasias Orbitárias/epidemiologia , Neoplasias Orbitárias/cirurgia , Prognóstico , Procedimentos de Cirurgia Plástica/métodos , Procedimentos de Cirurgia Plástica/tendências , Análise de Sobrevida , Resultado do TratamentoRESUMO
BACKGROUND: The use of conventional stains including Giemsa, Wright and Leishman have become an essential tool for differential diagnosis of cells in peripheral blood. AIMS: The aim of the study was to develop a new combination of Leishman-Giemsa (L&G) stain for avian blood smears and to compare its efficacy with conventional staining methods. METHODS: Three sets of peripheral blood smears, one smear for L&G stain and two other smears for Leishman and Giemsa stains, created from 50 broiler chickens blood samples. All the three sets of slides were blind screened by two expert clinical pathologists and scored based on the staining characteristics (4 parameters) such as nuclear features of red blood cell (RBC) and white blood cell (WBC), cytoplasmic features and cytoplasmic granularity of WBC. The average grading score assigned by two experts for each staining method were compared. RESULTS: The average grading score of two conventional Leishman and Giemsa staining methods were significantly lower (P<0.001) than new L&G staining method in avian nuclear features of the RBC and WBC. The L&G stain gave a better clarity of nuclear features of avian RBC and WBC. The new L&G staining technique created significant differences (P<0.001) in cytoplasmic features of avian WBC compared to the other two methods. CONCLUSIONS: For the first time, the results of the present study showed that the avian blood cells are more desirable stained with a new combination of L&G stain. In addition, it gives a better nuclear and cytoplasmic differential staining than the conventional Giemsa and Leishman stains when used alone.
RESUMO
The oro-ocular cleft number 5 according to the Tessier classification is one of the rarest facial clefts and few cases have been reported in the literature. Although the detailed structure of rare craniofacial clefts is well established, the cause of these pathological conditions is not. There are no existing guidelines for the management of this particular kind of cleft. We describe the case of a 19-month-old girl with a complete bilateral facial cleft. We describe the surgical steps taken to achieve the primary correction of the soft tissue deformation. Embryologic development and radiological approach are discussed, as are also the psychological and social aspects of severe facial deformities.
Assuntos
Fissura Palatina/cirurgia , Anormalidades do Olho/cirurgia , Face/anormalidades , Ossos Faciais/anormalidades , Ossos Faciais/cirurgia , Anormalidades da Boca/cirurgia , Fissura Palatina/diagnóstico por imagem , Anormalidades do Olho/diagnóstico por imagem , Ossos Faciais/diagnóstico por imagem , Feminino , Humanos , Lactente , Anormalidades da Boca/diagnóstico por imagem , Tomografia Computadorizada por Raios XRESUMO
OBJECTIVE: Charcot-Marie-Tooth neuropathy affects mainly and early the lower limbs, but hands deformities are a relevant problem, which involves the quality of life of the patients. Unfortunately, there are few studies about the evaluation of the upper limbs and very rare works about the rehabilitation. A treatment study at the moment is missing and it is important to search rehabilitation exercises to improve the dexterity and the quality of life of the patients. METHODS: We recruited 9 patients with clinical and genetic diagnosis of CMT and we proposed a rehabilitation protocol which includes muscle recruitment, stretching and proprioceptive exercises for the hand with the duration of 4 weeks (two sessions for week). We evaluated the patients before and one week after the treatment with Thumb Opposition Test, Sollerman Hand Function Scale, dynamometry (tripod pinch and hand grip). RESULTS: The rehabilitation protocol has been well tolerated and there were not dropouts. We did not observe any worsening in every scale we used. Every parameter tested showed an improvement especially in the right/dominant hand. CONCLUSION: This study demonstrates that this three phases treatment is well tolerated by patients, it is not detrimental for the hands status and perfectly reproducible by professionals. Moreover, this could be the basis for future randomized single blind projects.
RESUMO
Antibiotic resistance occurs in the endogenous flora of exposed population in addition to pathogenic bacteria. This study was conducted to evaluate the distribution of antibiotic resistance genes among 63 isolates of Escherichia coli of Escherichia coli (E. coli) in diarrheic calves and poultry. According to the results, B1 and B2 were the most prevalent phylogroups of E. coli in calves and poultry carcasses, respectively. Antimicrobial resistance was observed in 76% of the isolates, and 62% of the strains were multi-drug resistant. Antibiotic resistance in E. coli strains obtained from calves strains was significantly higher than those obtained from poultries. Additionally, the strains of B1 and D phylogroups had the highest and lowest antimicrobial resistance, respectively. At least one encoding gene for integrone was detected in 23 strains (36.5%) and Class I integron had the highest prevalence. Accordingly, this study gave baseline information on the magnitude of the resistance problem and its genetic background in E. coli from domesticated animals of the Tehran, Iran. Moreover, the power of oligonucleotide array technology in the discrimination of different genotypes during a short time was confirmed in this study.
Assuntos
Doenças dos Bovinos/epidemiologia , Galinhas , Diarreia/veterinária , Farmacorresistência Bacteriana/genética , Infecções por Escherichia coli/epidemiologia , Escherichia coli/genética , Doenças das Aves Domésticas/epidemiologia , Animais , Antibacterianos/farmacologia , Bovinos , Doenças dos Bovinos/microbiologia , Diarreia/epidemiologia , Diarreia/microbiologia , Escherichia coli/classificação , Escherichia coli/efeitos dos fármacos , Infecções por Escherichia coli/microbiologia , Proteínas de Escherichia coli/genética , Irã (Geográfico)/epidemiologia , Análise de Sequência com Séries de Oligonucleotídeos/veterinária , Doenças das Aves Domésticas/microbiologia , PrevalênciaRESUMO
PURPOSE: To assess demographic, clinical, radiological, pathological features, treatment and follow-up of periocular or/and orbital amyloidosis. PATIENTS AND METHODS: We conducted an observational retrospective monocentric study from January 2004 to April 2017 in patients diagnosed with histologically proven periocular or/and orbital amyloidosis. RESULTS: Six patients were included (2 females, 4 males). Mean age was 76.8 years (range 66-88 years). Mean time between first ophthalmological symptoms and diagnosis was 27 months (range 11-36 months). The main symptoms were subconjunctival infiltration (6 patients; 100%), periocular pain or discomfort (4 patients; 66.6%) and subconjunctival hemorrhage (1 patient; 16.6%). Clinical findings included ptosis (4 patients; 66.6%), keratitis (3 patients; 50%) leading to corneal perforation in one patient, and proptosis (3 patients; 50%). One-half of the patients showed bilateral involvement. AL amyloidosis was identified on immunohistochemistry in 5 patients (83.3%). One case of B cell marginal zone orbital lymphoma was diagnosed. Systemic work-up was negative for all patients. Treatment consisted of simple monitoring (1 patient; 16.6%), surgical debulking (3 patients; 50%), ptosis surgery (1 patient; 16.6%), eyelid or eyelash malposition surgery (2 patients; 33.3%) and orbital radiation beam therapy (2 patients; 33.3%). Mean follow-up was 14.6 months (range 6-36 months), and no progression nor recurrence were noted. CONCLUSION: Periocular or/and orbital amyloidosis is rarely encountered. Diagnosis is based on pathological examination, and immunohistochemistry analysis should always be performed to guide systemic work-up. Orbital lymphoma and multiple myeloma should be ruled out if AL amyloidosis is diagnosed. Progression is slow, and surgery is the mainstay of treatment in symptomatic patients. Long-term multidisciplinary follow-up is advocated.
Assuntos
Amiloidose/diagnóstico , Amiloidose/terapia , Doenças Orbitárias/diagnóstico , Doenças Orbitárias/terapia , Idoso , Idoso de 80 Anos ou mais , Amiloidose/patologia , Doenças da Túnica Conjuntiva/diagnóstico , Doenças da Túnica Conjuntiva/patologia , Doenças da Túnica Conjuntiva/terapia , Feminino , Seguimentos , Humanos , Masculino , Doenças Orbitárias/patologia , Estudos RetrospectivosRESUMO
The protective role of pentoxifylline (PTX) on sperm characteristics, reproductive hormones and histopathology following carrageenan-induced chronic nonbacterial prostatitis (CNP) was investigated in male Wistar rats. Thirty-six rats were grouped into six rats per group. Group 1 (control) received saline normal. Group 2 received a single intraprostatic dose of 3% carrageenan (50 µl) on day 1 (CNP). Groups 3 and 5 received cernilton (standard drug) and PTX orally at 100 and 50 mg/kg for 14 consecutive days respectively. Groups 4 and 6 received a single dose of 3% carrageenan (50 µl) intraprostatically on day 1 followed by cernilton and PTX orally at 100 and 50 mg/kg on the eighth day for 14 consecutive days respectively. Prostatic index, serum prostatic specific antigen, malondialdehyde, testosterone and luteinising hormone levels were significantly increased (p < .05), whereas serum follicle-stimulating hormone, sperm count, motility and viability were significantly decreased (p < .05) in CNP group. Histopathology of prostate revealed leucocyte infiltration, large involutions and projection into the lumen in CNP group and these aberrations were improved by PTX. According to these findings, we concluded that PTX effectively mitigated detrimental impact of CNP on sperm characteristics, reproductive hormones and histopathology in rats.
Assuntos
Pentoxifilina/farmacologia , Prostatite/tratamento farmacológico , Espermatozoides/efeitos dos fármacos , Testículo/efeitos dos fármacos , Animais , Carragenina , Hormônio Foliculoestimulante/sangue , Hormônio Luteinizante/sangue , Masculino , Pentoxifilina/uso terapêutico , Prostatite/induzido quimicamente , Prostatite/patologia , Ratos , Ratos Wistar , Motilidade dos Espermatozoides/efeitos dos fármacos , Espermatozoides/patologia , Testículo/patologia , Testosterona/sangueRESUMO
Background Graves' disease is an autoimmune disorder leading to hyperthyroidism. It is often associated with ophthalmic manifestations. Graves' disease is reported to be rare in the paediatric population. Patients and Methods We performed a retrospective analysis on all patients diagnosed with ophthalmopathy related to Graves' disease at a paediatric age (less than 18 years old) in our institution between 2004 and 2015. Results Eight patients were identified: 6 females and 2 males. The median age at diagnosis was 11.5 years (range 3-16). Ophthalmic signs were: proptosis (6/8), superficial punctate keratitis (5/8), eyelid retraction (4/8), lagophthalmos (2/8), and oculomotor dysfunction (2/8). No patients showed signs of complication such as ocular hypertension or compressive optic neuropathy. Orbital decompression was performed in one patient in a non-emergency setting. Conclusion Ophthalmic involvement in Graves' disease is rarely encountered in paediatric patients. Ophthalmologists should be aware of this entity to ensure that patients with thyroid dysfunction are identified at an early point in time.
Assuntos
Oftalmopatias/diagnóstico , Oftalmopatias/epidemiologia , Oftalmopatia de Graves/diagnóstico , Oftalmopatia de Graves/epidemiologia , Adolescente , Criança , Pré-Escolar , Comorbidade , Feminino , Humanos , Incidência , Masculino , Fatores de Risco , Suíça/epidemiologiaRESUMO
BACKGROUND: Dermoid cysts are frequent unilateral congenital benign tumors that can be found at different locations throughout the body. In the orbital region, dermoid cysts occur predominantly in the supero-temporal quadrant. However, different orbital locations are possible, making this entity an important differential diagnosis for orbital tumors. PATIENTS AND METHODS: We retrospectively reviewed the charts of 23 consecutive patients operated in our institution between 2005 and 2014 for orbital tumors that were diagnosed as congenital dermoid or epidermoid cysts. RESULTS: There were 21 dermoid and 2 epidermoid cysts. The median age at surgery time was 7 years (range 1-41). There were 15 females and 8 males. Eleven cysts were located supero-temporally (47.8%), seven supero-medially (30.4%), two temporally (8.7%), one at the frontal bone (4.4%) and two in the fossa of the lacrimal gland (8.7%). The mean cyst diameter was 13.1 mm±5.0 (SD). None had signs of malignant disease. No recurrence was observed after complete excision. CONCLUSION: Dermoid cysts of orbital region are mostly located in the supero-temporal quadrant. However, other orbital locations are possible and dermoid cysts must thus be considered in the differential diagnosis of any mass in the orbital region.
Assuntos
Cisto Dermoide/patologia , Neoplasias Orbitárias/patologia , Adolescente , Adulto , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Masculino , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Adulto JovemAssuntos
Antivirais/uso terapêutico , Dacriocistite/etiologia , Dacriocistite/prevenção & controle , Herpes Zoster Oftálmico/complicações , Herpes Zoster Oftálmico/tratamento farmacológico , Adulto , Dacriocistite/diagnóstico , Diagnóstico Diferencial , Herpes Zoster Oftálmico/diagnóstico , Humanos , Masculino , Resultado do TratamentoRESUMO
A very sensitive and convenient fluorescence nanobiosensor for rapid detection of DNA methylation based on Fe3O4/Au core/shell nanoparticles has been developed. Specific site of CpG islands of adenomatous polyposis coli (APC), a well studied tumor suppressor gene, was used as the detection target DNA sequence. The characteristics of nanoparticles were determined by scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive spectroscopy (EDS), UV-visible spectroscopy and X-ray diffraction (XRD) spectroscopy. Fe@Au nanoparticles functionalized by bounding of single stranded DNA (ssDNA) probe through sulfhydryl group at the 5' phosphate end. Then unmethylated and methylated complementary target ssDNA were hybridized with the immobilized ssDNA probe. Dipyridamole, a pharmaceutical agent used for the first time as a fluorescence probe which significantly interacted with hybridized unmethylated and methylated DNA. Upon the addition of the target unmethylated and methylated ssDNA, the fluorescence intensity increased in linear range by concentration of unmethylated ssDNA from 1.6 × 10(-15) to 6.6 × 10(-13)M with detection limit of 1.2 × 10(-16)M and on the other hand, fluorescence intensity declined linearly with concentration of 3.2 × 10(-15)-8.0 × 10(-13)M methylated DNA and detection limit was 3.1 × 10(-16)M. We have also shown that nanobiosensor could distinguish ratio of methylation in series of partially methylated DNA targets with identical sequences. A density functional theory (DFT) calculation was also performed to investigate the interaction between Dipyridamole with unmethylated and methylated cytosine. Finally real sample analysis suggested that nanobiosensor could have practical application for methylation detection in human plasma sample.
Assuntos
Técnicas Biossensoriais/instrumentação , Metilação de DNA/genética , DNA de Neoplasias/genética , Detecção Precoce de Câncer/instrumentação , Fluorometria/instrumentação , Nanotecnologia/instrumentação , Neoplasias/diagnóstico , Animais , DNA de Neoplasias/análise , DNA de Neoplasias/química , Desenho de Equipamento , Análise de Falha de Equipamento , Humanos , Neoplasias/genética , Reprodutibilidade dos Testes , Sensibilidade e Especificidade , Análise de Sequência de DNA/instrumentaçãoRESUMO
The aim of this study was to evaluate the influence of age and sex on the concentration of total serum protein measured by the biuret method and protein fractions determined using cellulose acetate electrophoresis in apparently healthy camels (Camelus dromedarius). Blood samples were collected from 21 camels (12 males and 9 females). The camels were further divided into two groups: 12 young camels at the age of 3 months to 2 years and 9 adult camels at the age of 3-15 years. Cellulose acetate electrophoresis of serum proteins identified five protein fractions in adult camels as young camels, these five protein fractions include albumin, α1 and α2, ß and γ-globulins. In adult camels, serum levels (g/l) of total protein, albumin, α1-globulins, α2-globulins, ß-globulins and γ-globulins were 80.9±3.10, 42.9±3.10, 1.3±0.22, 2.2±0.30, 11.8±0.30 and 22.6±0.20, respectively. However, in young camels, these levels (g/l) were 66.8±2.90, 40.2±2.40, 1.0±0.14, 2.6±0.30, 10.6±0.80 and 12.3±1.20, respectively. The albumin/globulin (A/G) ratio was 2.08±0.28 in adult camels and 3.77±0.53 in young ones. The mean serum concentrations of total protein and γ-globulins were significantly (P<0.05) higher and the A/G ratio was significantly lower in adult camels compared to young camels. The mean concentrations of γ-globulins were significantly higher and the A/G ratio was significantly (P<0.05) lower in females compared to male camels. The results of the present study indicate a significant effect of age and sex on the concentrations of some of the serum protein fractions in dromedary camels.
Assuntos
Antibacterianos/uso terapêutico , Conjuntivite/diagnóstico , Conjuntivite/terapia , Imunossupressores/uso terapêutico , Plasminogênio/deficiência , Plasminogênio/genética , Dermatopatias Genéticas/diagnóstico , Dermatopatias Genéticas/terapia , Pré-Escolar , Terapia Combinada , Conjuntivite/genética , Feminino , Predisposição Genética para Doença/genética , Humanos , Mutação/genética , Dermatopatias Genéticas/genética , Resultado do TratamentoRESUMO
Mild to moderate forms of orbitopathy are common in auto-immune thyroid diseases, whereas severe forms are rare. Euthyroidism restoration, no smoking, and ocular local lubricants are necessary for all the patients. In case of mild orbitopathy, treatment by selenium is now indicated. Active forms of thyroid orbitopathy are better treated by IV steroids. Surgery is indicated in optic neuropathy resistant to steroids and in sequellar forms of the disease.
Assuntos
Oftalmopatia de Graves/diagnóstico , Oftalmopatia de Graves/terapia , Antitireóideos/uso terapêutico , Descompressão Cirúrgica/métodos , Oftalmopatia de Graves/epidemiologia , Oftalmopatia de Graves/etiologia , Humanos , Procedimentos Cirúrgicos Oftalmológicos/métodos , Procedimentos Cirúrgicos Oftalmológicos/tendências , Complicações Pós-Operatórias/epidemiologia , Complicações Pós-Operatórias/etiologiaRESUMO
The present study was undertaken to investigate the effect of various levels of vitamin B12 in Tris extender on semen quality of Dallagh rams following the cooling and freeze/thawing process. Semen was collected from six healthy and mature rams with an average body weight of 60.0 ± 5.0 Kg using an electro ejaculator. High quality samples were mixed and diluted in Tris extender supplemented with different concentrations of vitamin B12 (0, 1, 2 and 3 mg/ml). The semen aliquots were cooled and preserved at 5°C and their qualities were evaluated during pre-freezing and then the cooled semen samples were packaged into 0.25 ml straws. Straws were frozen in the vapor of liquid nitrogen, and were then stored at -196ºC. Straws were thawed seven days later and the characteristics of spermatozoa were examined. Results of this study showed that the effect of vitamin B12 on characteristics such as viability, motility, progressive motility and normality of spermatozoa were significant in pre and post freezing conditions (P<0.05). In conclusion, for long term storage of semen of Dallagh rams, we recommend using 2 mg/ml of vitamin B12 in semen extender.