Compact freeform-surface-based Offner imaging spectrometer with both a long-slit and broadband.

Current imaging spectrometers with conventional optical elements face major challenges in achieving a large field of view (FOV), broadband and compact structure simultaneously. In this paper, a compact freeform-surface-based Offner imaging spectrometer with both a long-slit and a broadband (CISLS) is proposed. To keep a long slit and an anastigmatic imaging, the slit off-axis amount of the initial system is within a specific range theoretically. While to achieve a compact structure, the slit off-axis amount should be away from the specific range and as small as possible. Based on the vector aberration theory and the analytical study, Zernike polynomial terms Z5 and Z6 introduce the astigmatism independent of FOV. They are utilized to well balance the astigmatism when the slit off-axis amount is away from the specific range, helping a miniaturization of the system. Other Zernike polynomial terms below the eighth order introduce the astigmatism related to FOV. They contribute to balancing the astigmatism that produced with the increasing of the FOV, thus achieving a wide FOV. The design results show that the proposed CISLS with a high spectral resolution of 2.7 nm achieves a long slit of 30 mm in length but a small size of only 60 mm × 64 mm × 90 mm in volume under a broadband from 400 nm to 1000 nm.

The Causal Relationships Between Gut Microbiota, Brain Volume, and Intelligence: A Two-Step Mendelian Randomization Analysis.

BACKGROUND: Growing evidence indicates that dynamic changes in gut microbiome can affect intelligence; however, whether these relationships are causal remains elusive. We aimed to disentangle the poorly understood causal relationship between gut microbiota and intelligence. METHODS: We performed a 2-sample Mendelian randomization (MR) analysis using genetic variants from the largest available genome-wide association studies of gut microbiota (N = 18,340) and intelligence (N = 269,867). The inverse-variance weighted method was used to conduct the MR analyses complemented by a range of sensitivity analyses to validate the robustness of the results. Considering the close relationship between brain volume and intelligence, we applied 2-step MR to evaluate whether the identified effect was mediated by regulating brain volume (N = 47,316). RESULTS: We found a risk effect of the genus Oxalobacter on intelligence (odds ratio = 0.968 change in intelligence per standard deviation increase in taxa; 95% CI, 0.952-0.985; p = 1.88 × 10-4) and a protective effect of the genus Fusicatenibacter on intelligence (odds ratio = 1.053; 95% CI, 1.024-1.082; p = 3.03 × 10-4). The 2-step MR analysis further showed that the effect of genus Fusicatenibacter on intelligence was partially mediated by regulating brain volume, with a mediated proportion of 33.6% (95% CI, 6.8%-60.4%; p = .014). CONCLUSIONS: Our results provide causal evidence indicating the role of the microbiome in intelligence. Our findings may help reshape our understanding of the microbiota-gut-brain axis and development of novel intervention approaches for preventing cognitive impairment.

Multi-tissue transcriptome-wide association study reveals susceptibility genes and drug targets for insulin resistance-relevant phenotypes.

AIM: Genome-wide association studies (GWAS) have identified multiple susceptibility loci associated with insulin resistance (IR)-relevant phenotypes. However, the genes responsible for these associations remain largely unknown. We aim to identify susceptibility genes for IR-relevant phenotypes via a transcriptome-wide association study. MATERIALS AND METHODS: We conducted a large-scale multi-tissue transcriptome-wide association study for IR (Insulin Sensitivity Index, homeostasis model assessment-IR, fasting insulin) and lipid-relevant traits (high-density lipoprotein cholesterol, triglycerides, low-density lipoprotein cholesterol and total cholesterol) using the largest GWAS summary statistics and precomputed gene expression weights of 49 human tissues. Conditional and joint analyses were implemented to identify significantly independent genes. Furthermore, we estimated the causal effects of independent genes by Mendelian randomization causal inference analysis. RESULTS: We identified 1190 susceptibility genes causally associated with IR-relevant phenotypes, including 58 genes that were not implicated in the original GWAS. Among them, 11 genes were further supported in differential expression analyses or a gene knockout mice database, such as KRIT1 showed both significantly differential expression and IR-related phenotypic effects in knockout mice. Meanwhile, seven proteins encoded by susceptibility genes were targeted by clinically approved drugs, and three of these genes (H6PD, CACNB2 and DRD2) have been served as drug targets for IR-related diseases/traits. Moreover, drug repurposing analysis identified four compounds with profiles opposing the expression of genes associated with IR risk. CONCLUSIONS: Our study provided new insights into IR aetiology and avenues for therapeutic development.

Ultra-compact dual band imaging spectrometer with freeform prisms.

Wide spectrum and miniaturization are the main challenges in the imaging spectrometer design. In this paper, we propose an ultra-compact dual band imaging spectrometer (CDBIS) with cemented freeform prisms, which works at both the visible-near-infrared (VNIR) from 400 nm to 1000 nm and the shortwave-infrared (SWIR) from 1000 nm to 1700 nm. The imaging spectrometer is only composed of three cemented prisms, a primary prism and two triangular prisms. And a freeform surface characterized by the Zernike polynomial is introduced in each prism. The CDBIS is dispersed by a diffraction grating, which is designed on the second surface of the primary prism. Based on vector aberration theory (VAT), the relationship among the astigmatism generated by the introduced freeform surfaces, the wavelength, and the field of view is studied. Accordingly, a wideband is realized by introducing the freeform surfaces after the diffraction grating. Furthermore, through optimizing the coefficients of Zernike polynomial terms, residual astigmatism at different wavelengths is well balanced. An imaging spectrometer with a volume of only 100c m 3 is obtained, with a spectral resolution of 1.45 nm at VNIR and 2.40 nm at SWIR, respectively. It has a huge potential for broadband space exploration.

Analytical design of a cemented-curved-prism based integral field spectrometer (CIFS) with high numerical aperture and high resolution.

Snapshot hyperspectral imaging is superior to scanning spectrometers due to its advantage in dimensionality, allowing longer pixel dwell time and higher data cube acquisition efficiency. Due to the trade-off between spatial and spectral resolution in snapshot spectral imaging technologies, further improvements in the performance of snapshot imaging spectrometers are limited. Therefore, we propose a cemented-curved-prism-based integral field spectrometer (CIFS), which achieves high spatial and high spectral resolution imaging with a high numerical aperture. It consists of a hemispherical lens, a cemented-curved-prism and a concave spherical mirror. The design idea of aplanatic imaging and sharing-optical-path lays the foundation for CIFS to exhibit high-resolution imaging in a compact structure. The numerical model between the parameters of optical elements and the spectral resolution of the system is established, and we analyze the system resolution influenced by the hemispherical lens and the cemented-curved-prism. Thus, the refractive index requirements of the hemispherical lens and the cemented-curved-prism for the optimal spatial and spectral resolution imaging of the system are obtained, providing guidance for the construction of CIFS. The designed CIFS achieves pupil matching with a 1.8 f-number lenslet array, sampling 268 × 76 spatial points with 403 spectral channels in the wavelength band of 400 to 760 nm. The spectral and spatial resolution are further evaluated through a simulation experiment of spectral imaging based on Zemax. It paves the way for developing integral field spectrometers exhibiting high spatial and high spectral resolution imaging with high numerical aperture.

Non-uniformity correction of wide field of view imaging system.

Requirements for wide field of view (FOV) imaging system reflect the need for both uniform illumination as well as excellent image quality across the entire FOV. As the monocentric lens combined with a parallel array of relay imagers achieves a wide-FOV while maintaining a high resolution, we studied the monocentric cascade imaging system (MCIS). However, the imaging experiment of the prototype shows two issues, including vignetting and non-uniform image quality over the full FOV. They affect the image stitching which is necessary for wide-FOV image acquisition. This paper studies how the position of the aperture stop affects the vignetting and the local aberrations in MCIS. Moving laws of the aperture stop and its relationship with the local aberrations are presented. Moreover, aspheric surfaces on proper surfaces are introduced and studied to balance the local aberrations. Accordingly, an MCIS with uniform illumination and good image quality is presented. The MCIS achieves a wide-FOV of 116.4° and an instantaneous FOV of 0.0021°. It keeps a relative illumination exceeding 97% during the full FOV. The modulation transfer function (MTF) is over 0.285 at the Nyquist frequency of 270 lp/mm. This paper provides a profound theorical reference for further applications and developments of MCIS.

High spectral resolution compact Offner spectrometer based on the aberration-reduced convex holographic gratings recorded by spherical waves under Rowland circle mounting.

High spectral resolution, excellent imaging quality, and compact configuration have become a recent trend in push-broom imaging spectrometers. The concentric Offner imaging spectrometer has become popular due to its high optical performance and compactness. However, astigmatism is the dominant residual aberration in the Offner imaging spectrometer, which makes the meridional and sagittal images unable to be focused well and causes a deterioration in image quality and spectral resolution. In this paper, we present a compact Offner imaging spectrometer with a high resolution based on an aberration-reduced convex holographic grating (ACHG), which is recorded by spherical waves under Rowland circle mounting. The holographic aberration coefficients of ACHG and geometric aberration coefficients of the Offner imaging spectrometer are derived based on the analysis of the light-path function. Furthermore, we analyzed the relationship between holographic aberration coefficients and holographic recording parameters of ACHG under Rowland circle mounting. To balance the geometric aberration of the Offner imaging spectrometer, proper holographic aberration coefficients of the ACHG are achieved through adjusting the holographic recording parameters. The design result indicated that the Offner imaging spectrometer with ACHG provides better images than those with mechanically ruled convex grating (MRCG). Moreover, the spectral resolution is significantly improved. This lays down a theoretical basis for subsequent construction work in the Offner imaging spectrometer with holographic aberration-reduced gratings.

High diversity of Rickettsia spp., Anaplasma spp., and Ehrlichia spp. in ticks from Yunnan Province, Southwest China.

Rickettsia, Anaplasma, and Ehrlichia belonging to the order Rickettsiales are causative agents of tick-borne diseases in humans. During 2021, 434 ticks including Rhipicephalus microplus and R. haemaphysaloides were collected from three sampling sites in Yunnan Province, Southwest China, and analyzed for the presence of these bacteria. Nine bacterial species were identified, including two Rickettsia spp., three Anaplasma spp., and four Ehrlichia spp., some of which are potential human pathogens. Genetic and phylogenetic analysis on 16S rRNA, gltA, groEL, ompA, ompB, and sca4 genes indicated the presence of a novel spotted fever group Rickettsia (SFGR) named "Candidatus Rickettsia shennongii" in six of the 38 R. haemaphysaloides ticks from two locations, Dehong Autonomous Prefecture and Honghe City. Another SFGR species, Candidatus Rickettsia jingxinensis was detected in ticks from all three sites, with an overall positive rate of 62.67%. Three other human pathogenic species, Anaplasma ovis (1.38%, 6/434), Ehrlichia canis (16.36%, 71/434), and E. chaffeensis (0.23%, 1/434) were detected in these ticks and characterized. Moreover, Ehrlichia sp. (4.84%, 21/434), E. minasensis (7.37%, 32/434), A. marginale (6.91%, 30/434), and Cadidatus Anaplasma boleense (1.15%, 5/434) were detected in R. microplus ticks, for which pathogenicity to humans remains to be determined. The results reveal the remarkable diversity of Rickettsiales bacteria in ticks from Yunnan Province, Southwest China. The high infection rate of some human pathogenic bacteria in ticks may indicate potential infection risk in humans, and it highlights the need for surveillance in local populations.

Effectiveness of adenovirus type 5 vectored and inactivated COVID-19 vaccines against symptomatic COVID-19, COVID-19 pneumonia, and severe COVID-19 caused by the B.1.617.2 (Delta) variant: Evidence from an outbreak in Yunnan, China, 2021.

BACKGROUND: In partial response to the coronavirus disease 2019 (COVID-19) pandemic, countries around the world are conducting large-scale vaccination campaigns. Real-world estimates of vaccine effectiveness (VE) against the B.1.617.2 (Delta) variant are still limited. An outbreak in Ruili city of Chinaprovided an opportunity to evaluate VE against the Delta variant of two types of COVID-19 vaccines in use in China and globally - inactivated (CoronaVac and BBIBP-CorV) and adenovirus type 5 vectored (Convidecia) vaccines. METHODS: We estimated VE using a retrospective cohort study two months after the Ruili vaccination campaign (median: 63 days). Close contacts of infected people (Chinese nationality, 18 years and above) were included to assess VE against symptomatic Covid-19, COVID-19 pneumonia, and severe COVID-19. We calculated the relative risks (RR) of the outcomes for unvaccinated compared with fully vaccinated individuals. We used logistic regression analyses to estimate adjusted VEs, controlling for gender and age group (18-59 years and 60 years and over).We compared unvaccinated and fully vaccinated individuals on duration of RT-PCR positivity and Ct value. FINDINGS: There were 686 close contacts eligible for VE estimates. Adjusted VE ofad5-vectored vaccine was 61.5% (95% CI, 9.5-83.6) against symptomatic COVID-19, 67.9% (95%CI: 1.7-89.9) against pneumonia, and 100% (95%CI: 36.6-100) against severe/critical illness. For the two inactivated vaccines, combined VE was 74.6% (95% CI, 36.0-90.0) against symptomatic COVID-19, 76.7% (95% CI: 19.3-93.3) against pneumonia, and 100% (95% CI: 47.6-100) against severe/critical COVID-19. There were no statistically significant differences in VE between twoinactivated vaccines for symptomatic COVID-19 and for pneumonia, nor were there statistically significant differences between inactivated and ad5-vectored VE in any of the three outcomes. The median durations of RT-PCR positivity were 17 days for fifteen people vaccinated with an inactivated vaccine, 18 days for forty-four people vaccinated with the Ad5 vectored vaccine, and 26 days for eleven unvaccinated individuals. INTERPRETATION: These results provide reassuring evidence that the three vaccines are effective at preventing Delta-variant COVID-19 in short term following vaccination campaign, and are most effective at preventing more serious illness. The findings of reduced duration of RT-PCR positivity and length of hospital stay associated with full vaccination suggests potential saving of health-care system resources.

[Epidemiological survey of suspected natural foci of schistosomiasis in Luxi City].

Three schistosomiasis patients pathologically diagnosed were detected in Luxi City, Yunnan Province, a non-endemic area of schistosomiasis. All the cases were hospitalized for serious intestinal diseases, and Schistosoma japonicum eggs were detected under their colonic mucosa. The 3 patients all denied they had contacted with infested water outside their resident place, and it was suggested that they might be infected in the local area.