Protective efficacy of dietary natural antioxidants on microplastic particles-induced histopathological lesions in African catfish (Clarias gariepinus).

Microplastic particles (MPs) are a common environmental pollutant easily ingested by fish in aquaculture. The current study evaluated the protective efficacies of some antioxidant, e.g., lycopene, citric acid, and chlorella, against the toxic effects of MP ingestion by Clarias gariepinus using histopathological biomarkers. Five experimental groups were established, a control group receiving only a standard diet, a group exposed to 500 mg/kg MP concomitant with the standard diet, and three antioxidant groups exposed to MPs plus either lycopene (500 mg/kg), citric acid (30 g/kg), or chlorella (50 g/kg) in the standard diet. After 15 days, fish were sacrificed for histological and histochemical examinations. Histological analysis of the kidney for group 2 (fed 500 mg/kg MPs alone) revealed distributed tissue dissociation, regional glomerular hypertrophy or shrinkage, melanomacrophage accumulation, and expansion of Bowman's space, while liver tissue exhibited dilation and rupture of the central vein wall, hemorrhage, cytoplasmic vacuolation, and cellular necrosis or apoptosis. Fish exposed to MPs also exhibited connective tissue fiber accumulation around renal blood vessels, renal tubules, the central hepatic vein, hepatic blood sinusoids, and serosal, muscle, and submucosal layers of the intestine. In addition, MP exposure reduced carbohydrate (mainly glycogen) contents in the brush borders and basement membranes of renal tubules, glomeruli, and intestinal tissues as well as in the cytoplasm of hepatocytes. These signs of renal, hepatic, and intestinal histopathology were fully or partially reversed by dietary lycopene, chlorella, or citric acid. Enhancing dietary antioxidants is an effective strategy for preventing MP toxicity in Clarias gariepinus in aquaculture.

Cytotoxic and hemotoxic effects of silver nanoparticles on the African Catfish, Clarias gariepinus (Burchell, 1822).

The present study investigates the hemotoxic and cytotoxic impacts of two acute doses of silver nanoparticles (AgNPs) on the African catfish, Clarias garepinus in comparison to the impact of AgNO3 and the control fish. AgNPs-induced impacts were recorded on some biological and hematological indices of that species on the bases of their size (20 and 40 nm) and concentration (10 and 100 µg) but no significant interaction. AgNO3 had very low impact on these indices in comparison to AgNPs. Recovery period for 15 days was found to be valid to remove AgNPs and AgNO3 toxicity for most indices. The condition factor exhibited stability under stress whereas the hepatosomatic index was more sensitive to AgNPs. The AgNPs-induced hematological changes recorded were corresponding with different blood cell alterations which increased in frequency from the control and AgNO3 to 40 nm/100 µg; such blood cell alterations disappeared to great extent after recovery period of 15-days in a reverse order.