Impact of sampling techniques on measured stormwater quality data for small streams.

Science-based sampling methodologies are needed to enhance water quality characterization for setting appropriate water quality standards, developing Total Maximum Daily Loads, and managing nonpoint source pollution. Storm event sampling, which is vital for adequate assessment of water quality in small (wadeable) streams, is typically conducted by manual grab or integrated sampling or with an automated sampler. Although it is typically assumed that samples from a single point adequately represent mean cross-sectional concentrations, especially for dissolved constituents, this assumption of well-mixed conditions has received limited evaluation. Similarly, the impact of temporal (within-storm) concentration variability is rarely considered. Therefore, this study evaluated differences in stormwater quality measured in small streams with several common sampling techniques, which in essence evaluated within-channel and within-storm concentration variability. Constituent concentrations from manual grab samples and from integrated samples were compared for 31 events, then concentrations were also compared for seven events with automated sample collection. Comparison of sampling techniques indicated varying degrees of concentration variability within channel cross sections for both dissolved and particulate constituents, which is contrary to common assumptions of substantial variability in particulate concentrations and of minimal variability in dissolved concentrations. Results also indicated the potential for substantial within-storm (temporal) concentration variability for both dissolved and particulate constituents. Thus, failing to account for potential cross-sectional and temporal concentration variability in stormwater monitoring projects can introduce additional uncertainty in measured water quality data.

Gene deletion of inositol hexakisphosphate kinase 2 predisposes to aerodigestive tract carcinoma.

Inositol hexakisphosphate kinase 2 (IP6K2), a member of the inositol hexakisphosphate kinase family, functions as a growth suppressive and apoptosis-enhancing kinase during cell stress. We created mice with a targeted deletion of IP6K2; these mice display normal embryogenesis, development, growth and fertility. Chronic exposure to the carcinogen 4-nitroquinoline 1-oxide (4-NQO, a UV-mimetic compound) in drinking water resulted in fourfold increased incidence of invasive squamous cell carcinoma (SCC) formation in the oral cavity and esophagus of the knockout (KO) mice compared to the wild-type (WT) littermates. Paradoxically, KO mice displayed relative resistance to ionizing radiation and exhibit enhanced survival following 8-10 Gy total body irradiation. Primary KO fibroblasts displayed resistance to antiproliferative effects of interferon-beta and increased colony forming units following ionizing radiation. Radioresistance of KO fibroblasts was associated with accelerated DNA repair measured by comet assay. Direct microinjection of 5-PP-Ins(1,2,3,4,6)P(5) (the enzymatic product of IP6K2), but not InsP(6) (the substrate of IP6K2) induced cell death in SCC22A squamous carcinoma cells.

A cryptic lineage within the pupfish Cyprinodon dearborni suggests multiple colonizations of South America.

The coastal South American species Cyprinodon dearborni contains two lineages distinct at both mitochondrial and nuclear loci. One appears to be a long-term South American endemic, whereas the other is a more recent colonizer related to the widespread Cyprinodon variegatus.

Cellular membranes and lipid-binding domains as attractive targets for drug development.

Interdisciplinary research focused on biological membranes has revealed them as signaling and trafficking platforms for processes fundamental to life. Biomembranes harbor receptors, ion channels, lipid domains, lipid signals, and scaffolding complexes, which function to maintain cellular growth, metabolism, and homeostasis. Moreover, abnormalities in lipid metabolism attributed to genetic changes among other causes are often associated with diseases such as cancer, arthritis and diabetes. Thus, there is a need to comprehensively understand molecular events occurring within and on membranes as a means of grasping disease etiology and identifying viable targets for drug development. A rapidly expanding field in the last decade has centered on understanding membrane recruitment of peripheral proteins. This class of proteins reversibly interacts with specific lipids in a spatial and temporal fashion in crucial biological processes. Typically, recruitment of peripheral proteins to the different cellular sites is mediated by one or more modular lipid-binding domains through specific lipid recognition. Structural, computational, and experimental studies of these lipid-binding domains have demonstrated how they specifically recognize their cognate lipids and achieve subcellular localization. However, the mechanisms by which these modular domains and their host proteins are recruited to and interact with various cell membranes often vary drastically due to differences in lipid affinity, specificity, penetration as well as protein-protein and intramolecular interactions. As there is still a paucity of predictive data for peripheral protein function, these enzymes are often rigorously studied to characterize their lipid-dependent properties. This review summarizes recent progress in our understanding of how peripheral proteins are recruited to biomembranes and highlights avenues to exploit in drug development targeted at cellular membranes and/or lipid-binding proteins.

A multi-locus assessment of connectivity and historical demography in the bluehead wrasse (Thalassoma bifasciatum).

The pelagic larval stage of most coral reef fishes might allow extensive dispersal or, alternatively, some level of local recruitment might be important. Molecular markers can be used to obtain indirect estimates of dispersal to evaluate these alternatives, yet the extent of gene flow among populations is known for only a small number of species. The use of such markers must take into account the properties of the markers and the demographic history of the population when making inferences about current gene flow. In the Caribbean bluehead wrasse, Thalassoma bifasciatum, previous studies have found both substantial levels of local recruitment, in studies interpreting otolith microchemistry and, conversely, a lack of genetic differentiation inferred from mitochondrial DNA (mtDNA) restriction-fragment length polymorphism (RFLP) data and allozymes. However, if subtle differentiation exists, larger sample sizes and highly variable markers may be required to discern it. Here we present results from mitochondrial control region sequence and microsatellite data that indicate a lack of genetic differentiation at both small and large spatial scales. However, historical processes, such as changes in population size, may have affected the current distribution of genetic variation.

Neutralization potential determination of siderite (FeCO3) using selected oxidants.

Siderite (FeCO3) is commonly found in coal overburden and, when present, can cause interference in the determination of neutralization potential (NP). Under acidic testing conditions, FeCO3 reacts to neutralize acid, which contributes to the NP. However, continued weathering of FeCO3 (oxidation of Fe2+ and hydrolysis of Fe3+) produces a neutral to slightly acidic solution. The effects of hydrogen peroxide (H2O2), potassium permanganate (KMnO4), and O2 on the laboratory measurement of NP of siderite samples taken from overburden were examined. All oxidation treatments lowered the NP values of the siderite samples as compared with the standard USEPA method. However, oxidation with H2O2 produced variable results depending on the amount of H2O2 added. Neutralization potential values obtained after oxidation treatments were highly correlated with Mn concentration. Reaction products (i.e., 2-line ferrihydrite) of siderite samples with H2O2 and KMnO4 were not representative of natural siderite weathering. Oxidation with O2 produced the lowest NP values for siderite samples. The reaction products produced by oxidation with O2 most closely represent those intermediate products formed when siderite is exposed to atmospheric weathering conditions. Oxidation with O2 also proved to be the most reproducible method for accurately assessing NP when siderite is present in overburden samples.

Water quality impacts of converting to a poultry litter fertilization strategy.

When improperly managed, land application of animal manures can harm the environment; however, limited watershed-scale runoff water quality data are available to research and address this issue. The water quality impacts of conversion to poultry litter fertilization on cultivated and pasture watersheds in the Texas Blackland Prairie were evaluated in this three-year study. Edge-of-field N and P concentrations and loads in surface runoff from new litter application sites were compared with losses under inorganic fertilization. The impact on downstream nutrient loss was also examined. In the fallow year with no fertilizer application, nutrient losses averaged 3 kg N ha(-1) and 0.9 kg P ha(-1) for the cultivated watersheds and were below 0.1 kg ha(-1) for the pasture watersheds. Following litter application, PO(4)-P concentrations in runoff were positively correlated to litter application rate and Mehlich-3 soil P levels. Following litter application, NO(3)-N and NH(4)-N concentrations in runoff were typically greater from cultivated watersheds, but PO(4)-P concentrations were greater for the pasture watersheds. Total N and P loads from the pasture watersheds (0.2 kg N ha(-1) and 0.7 kg P ha(-1)) were significantly lower than from the cultivated watersheds (32 kg N ha(-1) and 5 kg P ha(-1)) partly due to lower runoff volumes from the pasture watersheds. Downstream N and P concentrations and per-area loads were much lower than from edge-of-field watersheds. Results demonstrate that a properly managed annual litter application (4.5 Mg ha(-1) or less depending on litter N and P content) with supplemental N should supply necessary nutrients without detrimental water quality impacts.

Testing paleolimnological predictions with molecular data: the origins of Holarctic Eubosmina.

Zooplankton of the family Bosminidae have a unique paleolimnological record in many Holarctic lakes that provides a near continuous record of morphological change for thousands of years. If this morphological change could be interpreted reliably, then a rarely achieved direct observation of macroevolution would be feasible. We tested paleolimnological predictions derived from morphological variation found in the genus Eubosmina using mtDNA and nuclear DNA sequence variation from geographically distant Holarctic sites. The mtDNA and nDNA trees were congruent but genetic divergence was inversely associated with morphological divergence. The three most genetically divergent groups belonged to Eubosmina longispina, whose phylogeography and genetic divergence was consistent with glacial vicariance. The genetic evidence also supported the hypothesis that at least two Nearctic species were recent European introductions. Finally, the genetic evidence was consistent with paleolimnology in the finding of several proposed species undergoing rapid morphological evolution and being post-glacially derived from European E. longispina. The results suggested that lacustrine bosminids are susceptible to geographic speciation processes, and that morphological interpretation of diversity in paleolimnology can be markedly improved by genetic studies.

Effect of roundup ultra on microbial activity and biomass from selected soils.

Herbicides applied to soils potentially affect soil microbial activity. The quantity and frequency of Roundup Ultra [RU; N-(phosphonomethyl)glycine; Monsanto, St. Louis, MO] applications have escalated with the advent of Roundup-tolerant crops. The objective of this study was to determine the effect of Roundup Ultra on soil microbial biomass and activity across a range of soils varying in fertility. The isoproplyamine salt of glyphosate was applied in the form of RU at a rate of 234 mg active ingredient kg(-1) soil based on an assumed 2-mm glyphosate-soil interaction depth. Roundup Ultra significantly stimulated soil microbial activity as measured by C and N mineralization, as well as soil microbial biomass. Cumulative C mineralization as well as mineralization rate increased above background levels for all soils tested with addition of RU. There were strong linear relationships between C and N mineralized, as well as between soil microbial C and N (r2 = 0.96 and 0.95, respectively). The slopes of the relationships with RU addition approximated three. Since the isopropylamine salt of glyphosate has a C to N ratio of 3:1, the data strongly suggest that RU was the direct cause of the enhanced microbial activity. An increase in the C mineralization rate occurred the first day following RU addition and continued for 14 d. Roundup Ultra appeared to be rapidly degraded by soil microbes regardless of soil type or organic matter content, even at high application rates, without adversely affecting microbial activity.

Variability of particle size-specific fractions of personal coal mine dust exposures.

This study estimated the ratio of the tracheo-bronchial dust fraction to the fraction collected by a respirable dust sampler for a variety of job classifications found in conventional, continuous, and longwall coal mining sections. The ratios could then be applied in epidemiologic studies to existing respirable dust measurements to estimate thoracic mass concentrations for evaluation of the relative importance of the respirable and thoracic dust fractions to obstructive lung disease. Data collected include particle size distributions from four U.S. underground coal mines using eight-stage personal cascade impactors. A total of 180 samples were examined by mine, occupation and occupations grouped by proximity to the mine face, and by mining technology. Several fractions--that collected by the 10-mm nylon cyclone, the American Conference of Governmental Industrial Hygienists respirable and thoracic particulate mass fractions, and the estimated alveolar and tracheo-bronchial deposition fractions--were estimated. These were not significantly different when grouped by occupation, by proximity of work to the mine face, or by the type of mining technology in use. Distributions from one mine varied from the others, perhaps because it used diesel equipment in the haulage ways, which contributed to the fine aerosol fractions. Results suggest that although the tracheo-bronchial dust fraction may contribute to the development of obstructive lung disease, occupation-specific tracheo-bronchial dust fractions are not likely to produce stronger exposure-response estimates than the historically collected respirable dust concentrations.

Roentgenographic dimensions of the upper airway in snoring patients with and without obstructive sleep apnea.

The purpose of this study was to examine whether a simple test, such as routine roentgenographic views of the upper airway, is useful in identifying anatomic narrowing of the airway in patients with sleep apnea. To accomplish this, we prospectively studied a group of 117 patients (95 male and 22 female subjects) referred for evaluation of heavy snoring and possible obstructive sleep apnea. All patients had full nocturnal polysomnography, including measurements of snoring. Lateral view of the airway obtained after swallowing contrast material was used to measure pharyngeal diameters at three sites along the airway. All measurements were performed with the patients standing and supine. We used three different definitions of sleep apnea (apnea/hypopnea index of 10, 20, and 40), and compared airway diameters between the apneic and nonapneic snorers. Only when sleep apnea was defined as greater than 40 apneas plus hypopneas per hour of sleep was there a significant difference in airway diameter at the tip of the palate and 1 cm distal to it between apneic and nonapneic snorers. Both groups of patients demonstrated a significant reduction in the retropalatal distance on assumption of the supine posture. Stepwise, forward, multiple linear regression analysis showed that the retropalatal distance and airway diameter at the tip of the palate and 1 cm distal to it were significant predictors of snoring, but not apnea. We conclude that (1) airway diameters account for some of the variability in snoring, and (2) they do not differentiate between apneic and nonapneic snorers.

Self-selected exercise intensity is unchanged by sleep loss.

Sleep loss alters the perceptual response to exercise: subjects describe constant external work loads as more severe after sleep deprivation. However, since subjects cannot be "blind" to their sleep status and have knowledge of previous exertional ratings, it remains unknown if this increase in perceived exertion merely represents expectations of increased difficulty of exercise after sleep loss. As one approach to this problem, we asked 24 subjects to produce equivalent "very hard" efforts, once after normal sleep, and once after 30 h without sleep. This was done by allowing the subject, while walking at constant speed, to adjust treadmill grade, without knowledge of the actual elevation. We found that exercise at equal perceived exertion was associated with the choice of a nearly equal absolute work load after sleep deprivation as after normal sleep (17.1 vs. 17.5% grade; p = n.s.). In addition, after 10 min of exercise at the self-selected intensity, subjects displayed identical ventilation, oxygen uptake, and CO2 production. However, heart rate was significantly lower during exercise after sleep loss (170 +/- 3 vs. 178 +/- 3 b X min-1; p less than 0.001). These results suggest that previously measured increases in perceived exertion during constant-load exercise after sleep loss may be spurious.

Two-dimensional echocardiographic assessment of vegetative endocarditis.

Real-time, two-dimensional echocardiography was used to document the presence and assess the size and location of vegetative lesions of the cardiac valves and chambers in seven patients with bacterial endocarditis. Anatomic correlation (surgical or autopsy) was accurate in all patients. Two-dimensional echocardiography was shown to be of particular value in determining morphologic characteristics of the lesions since this technique provides spatial information concerning moving cardiac structures. The results of two-dimensional echocardiography were most helpful in determining selected aspects of the clinical care provided for each patient. These data demonstrate that real-time, two-dimensional echocardiography, in combination with M-mode imaging techniques, may be of great potential value in the detection of vegetative endocarditis. Furthermore, reliable assessment of lesion size and location may, in time, become a valuable method for following the morphologic changes that occur in vegetative lesions, and thus determine the efficacy of antibiotic therapy. This information may also provide a means for evaluating certain patients for surgical intervention.

Echocardiographic evaluation of tricuspid valve endocarditis: an M mode and two dimensional study.

Standard M mode echocardiography and a new real time two dimensional echocardiographic imaging system were utilized to follow the progressive anatomic destruction of the tricuspid valve in a patient with bacterial endocarditis. The initial two dimensional echocardiographic study revealed large vegetative masses attached to severely prolapsing tricuspid leaflets. Serial studies demonstrated eventual disruption of the chordal attachments of the anterior tricuspid leaflet resulting in frank leaflet flail. This technique was seen to complement both M mode echocardiography and cardiac angiography by providing spatial information concerning serial changes in the disordered tricuspid valve. Such findings call attention to the relative roles of various diagnostic measures, including M mode and two dimensional echocardiography, in assessing the specific anatomic and functional performance of a diseased tricuspid valve.