Cloning, characterization, and spatio-temporal expression patterns of HdhSPARC and its responses to multiple stressors.

SPARC is an extracellular Ca2+-binding, secreted glycoprotein that plays a dynamic role in the growth and development of organisms. This study aimed to describe the isolation, characterization, and expression analysis of HdhSPARC in Pacific abalone (Haliotis discus hannai) to infer its potential functional role. The isolated HdhSPARC was 1633 bp long, encoding a polypeptide of 284 amino acid residues. Structurally, the SPARC protein in abalone is comprised of three biological domains. However, the structure of this protein varied between vertebrates and invertebrates, as suggested by their distinct clustering patterns in phylogenetic analysis. In early development, HdhSPARC was variably expressed, and higher expression was found in veliger larvae. Moreover, HdhSPARC was highly expressed in juvenile abalone with rapid growth compared to their slower-growing counterparts. Among the testicular development stages, the growth stage exhibited higher HdhSPARC expression. HdhSPARC was also upregulated during muscle remodeling and shell biomineralization, as well as in response to different stressors such as heat shock, LPS, and H2O2 exposure. However, this gene was downregulated in Cd-exposed abalone. The present study first comprehensively characterized the HdhSPARC gene, and its spatio-temporal expressions were analyzed along with its responses to various stressors.

The Neuropeptide HGAP Regulates Growth, Reproduction, Metamorphosis, Tissue Damage Repair, and Response against Starvation in Pacific Abalone.

INTRODUCTION: Neuropeptides regulate vital physiological processes in multicellular organisms, including growth, reproduction, metamorphosis, and feeding. Recent transcriptome analyses have revealed neuropeptide genes with potential roles in vertebrate and invertebrate growth and reproduction. Among these genes, haliotid growth-associated peptide (HGAP) was identified as a novel gene in abalone. METHODS: This study focused on HGAP in Pacific abalone (Haliotis discus hannai), where the complete cDNA sequence named Hdh-HGAP was identified and characterized. Samples from different experiments, such as metamorphosis, juvenile abalone growth, gonad development stages, muscle remodeling, and starvation, were collected for mRNA expression analysis. RESULTS: The sequence spans 552 bp, encoding 96 amino acids with a molecular weight of 10.96 kDa. Expression analysis revealed that Hdh-HGAP exhibited higher levels in muscle tissue. Notably, during metamorphosis, Hdh-HGAP exhibited greater expression in the trochophore, veliger, and juvenile stages than in the cell division stages. Regarding growth patterns, Hdh-HGAP was highly expressed during rapid growth compared to stunted, minimal, and normal growth. In gonadal development, Hdh-HGAP mRNA reached its highest expression level during the ripening stage, indicating a potential role in gonadal cell proliferation and maturation. The in vivo effects of GnRH on gonad development and the expression of the Hdh-HGAP neuropeptide indicate its involvement in regulating reproduction in Pacific abalone. While tissue remodeling is primarily governed by immune genes, Hdh-HGAP was also upregulated during muscle tissue remodeling. Conversely, Hdh-HGAP was downregulated during prolonged starvation. CONCLUSION: This study marks the first comprehensive exploration of the Hdh-HGAP neuropeptide gene in Pacific abalone, shedding light on its involvement in growth, reproduction, metamorphosis, tissue remodeling, and response to starvation, although regulatory mechanisms are mostly unknown.

EF-Hand-Binding Secreted Protein Hdh-SMP5 Regulates Shell Biomineralization and Responses to Stress in Pacific Abalone, Haliotis discus hannai.

The development of a shell is a complex calcium metabolic process involving shell matrix proteins (SMPs). In this study, we describe the isolation, characterization, and expression of SMP5 and investigate its potential regulatory role in the shell biomineralization of Pacific abalone Haliotis discus hannai. The full-length Hdh-SMP5 cDNA contains 685 bp and encodes a polypeptide of 134 amino acids. Structurally, the Hdh-SMP5 protein belongs to the EF-hand-binding superfamily, which possesses three EF-hand Ca2+-binding regions and is rich in aspartic acid. The distinct clustering patterns in the phylogenetic tree indicate that the amino acid composition and structure of this protein may vary among different SMPs. During early development, significantly higher expression was observed in the trochophore and veliger stages. Hdh-SMP5 was also upregulated during shell biomineralization in Pacific abalone. Long periods of starvation cause Hdh-SMP5 expression to decrease. Furthermore, Hdh-SMP5 expression was observed to be significantly higher under thermal stress at temperatures of 15, 30, and 25 °C for durations of 6 h, 12 h, and 48 h, respectively. Our study is the first to characterize Hdh-SMP5 comprehensively and analyze its expression to elucidate its dynamic roles in ontogenetic development, shell biomineralization, and the response to starvation and thermal stress.

Selectivity of Sol-Gel and Hydrothermal TiO2 Nanoparticles towards Photocatalytic Degradation of Cationic and Anionic Dyes.

Titanium dioxide (TiO2) nanoparticles have been extensively studied for catalyzing the photo-degradation of organic pollutants, the photocatalyst being nonselective to the substrate. We, however, found that TiO2 nanoparticles prepared via the sol-gel and hydrothermal synthetic routes each possess a definite specificity to the charge of the substrate for photodegradation. The nanoparticles were characterized by SEM, FTIR, XRD, TGA, and UV-visible spectra, and the photocatalytic degradation under UV-B (285 nm) irradiation of two model compounds, anionic methyl Orange (MO) and cationic methylene blue (MB) was monitored by a UV-visible spectrophotometer. Untreated sol-gel TiO2 nanoparticles (Tsg) preferentially degraded MO over MB (90% versus 40% in two hours), while after calcination at 400 °C for two hours (Tsgc) they showed reversed specificity (50% MO versus 90% MB in one hour). The as-prepared hydrothermal TiO2 nanoparticles (Tht) behaved in the opposite sense of Tsg (41% MO versus 91% MB degraded in one and a half hours); calcination at 400 °C (Thtc) did not reverse the trend but enhanced the efficiency of degradation. The study indicates that TiO2 nanoparticles can be made to degrade a specific class of organic pollutants from an effluent facilitating the recycling of a specific class of pollutants for cost-effective effluent management.

Glutathione reductase, a biomarker of pollutant and stress in Pacific abalone.

Abalone are frequently exposed to several environmental factors including heavy metal toxicity, thermal stress, H2O2-stress, starvation, viral and bacterial infection that can induce oxidative stress. Glutathione reductase is a vital enzyme in the antioxidant defense system that catalyzes the reduction of oxidized glutathione to reduced glutathione. The present study aimed to identify and localize glutathione reductase in Pacific abalone (Hdh-GR) and assess its potential role in stress physiology, heavy metal toxicity, immune response, gonadal development, and metamorphosis. The mRNA expression of Hdh-GR was upregulated in response to thermal stress, starvation, H2O2-stress, and cadmium-exposed toxicity. The induced mRNA expression was also quantified in immune-challenged abalone. Moreover, the Hdh-GR expression was significantly higher during metamorphosis. The Hdh-GR mRNA expression showed an inverse relationship with ROS production in heat stressed Pacific abalone. These results suggest that Hdh-GR has central role in the stress physiology, immune response, gonadal development, and metamorphosis of Pacific abalone.

Fabrication of Robust and Stable N-Doped ZnO/Single-Walled Carbon Nanotubes: Characterization, Photocatalytic Application, Kinetics, Degradation Products, and Toxicity Analysis.

The production of effective visible-light (VL) photocatalysts for the elimination of noxious organic pollutants from wastewater has attracted considerable interest owing to increasing awareness worldwide. Despite the large number of photocatalysts reported, the selectivity and activity of photocatalysts still need to be developed. The goal of this research is to eliminate toxic methylene blue (MB) dye from wastewater through a cost-effective photocatalytic process using VL illumination. A novel N-doped ZnO/carbon nanotube (NZO/CNT) nanocomposite was successfully synthesized via a facile cocrystallization method. The structural, morphological, and optical properties of the synthesized nanocomposite were systematically investigated. The as-prepared NZO/CNT composite exhibited remarkable photocatalytic performance (96.58%) within 25 min of VL irradiation. The activity was 92, 52, and 27% greater than that of photolysis, ZnO, and NZO, respectively, under identical conditions. The enhanced photocatalytic efficiency of NZO/CNT was attributed to the N atom and CNT involvement: N contributes to narrowing the band gap of ZnO, and CNT captures the electrons and maintains the electron flow in the system. The reaction kinetics of MB degradation, catalyst reusability, and stability were also investigated. In addition, the photodegradation products and their toxicity effects in our environment were analyzed using the liquid chromatography-mass spectrometry and ecological structure activity relationships programs, respectively. The findings of the current study demonstrate that the NZO/CNT nanocomposite can be utilized to remove contaminants in an environmentally acceptable manner, thereby providing a new window for practical applications.

Fish waste to sustainable additives: Fish protein hydrolysates alleviate intestinal dysbiosis and muscle atrophy induced by poultry by-product meal in Lates calcarifer juvenile.

Valorising waste from the processing of fishery and aquaculture products into functional additives, and subsequent use in aquafeed as supplements could be a novel approach to promoting sustainability in the aquaculture industry. The present study supplemented 10% of various fish protein hydrolysates (FPHs), obtained from the hydrolysis of kingfish (KH), carp (CH) and tuna (TH) waste, with 90% of poultry by-product meal (PBM) protein to replace fishmeal (FM) completely from the barramundi diet. At the end of the trial, intestinal mucosal barriers damage, quantified by villus area (VA), lamina propria area (LPA), LPA ratio, villus length (VL), villus width (VW), and neutral mucin (NM) in barramundi fed a PBM-based diet was repaired when PBM was supplemented with various FPHs (p < 0.05, 0.01, and 0.001). PBM-TH diet further improved these barrier functions in the intestine of fish (p < 0.05 and 0.001). Similarly, FPHs supplementation suppressed PBM-induced intestinal inflammation by controlling the expression of inflammatory cytokines (tnf-α and il-10; p < 0.05 and 0.001) and a mucin-relevant production gene (i-mucin c; p < 0.001). The 16S rRNA data showed that a PBM-based diet resulted in dysbiosis of intestinal bacteria, supported by a lower abundance of microbial diversity (p < 0.001) aligned with a prevalence of Photobacterium. PBM-FPHs restored intestine homeostasis by enhancing microbial diversity compared to those fed a PBM diet (p < 0.001). PBM-TH improved the diversity (p < 0.001) further by elevating the Firmicutes phylum and the Ruminococcus, Faecalibacterium, and Bacteroides genera. Muscle atrophy, evaluated by fiber density, hyperplasia and hypertrophy and associated genes (igf-1, myf5, and myog), occurred in barramundi fed PBM diet but was repaired after supplementation of FPHs with the PBM (p < 0.05, 0.01, and 0.001). Similarly, creatine kinase, calcium, phosphorous, and haptoglobin were impacted by PBM-based diet (p < 0.05) but were restored in barramundi fed FPHs supplemented diets (p < 0.05 and 0.01). Hence, using circular economy principles, functional FPHs could be recovered from the fish waste applied in aquafeed formulations and could prevent PBM-induced intestinal dysbiosis and muscular atrophy. GRAPHICAL ABSTRACT.

Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone.

Catalase is a crucial enzyme of the antioxidant defense system responsible for the maintenance of cellular redox homeostasis. The aim of the present study was to evaluate the molecular regulation of catalase (Hdh-CAT) in stress physiology, innate immunity, testicular development, metamorphosis, and cryopreserved sperm of Pacific abalone. Hdh-CAT gene was cloned from the digestive gland (DG) of Pacific abalone. The 2894 bp sequence of Hdh-CAT had an open reading frame of 1506 bp encoding 501 deduced amino acids. Fluorescence in situ hybridization confirmed Hdh-CAT localization in the digestive tubules of the DG. Hdh-CAT was induced by different types of stress including thermal stress, H2O2 induction, and starvation. Immune challenges with Vibrio, lipopolysaccharides, and polyinosinic-polycytidylic acid sodium salt also upregulated Hdh-CAT mRNA expression and catalase activity. Hdh-CAT responded to cadmium induced-toxicity by increasing mRNA expression and catalase activity. Elevated seasonal temperature also altered Hdh-CAT mRNA expression. Hdh-CAT mRNA expression was relatively higher at the trochophore larvae stage of metamorphosis. Cryopreserved sperm showed significantly lower Hdh-CAT mRNA expression levels compared with fresh sperm. Hdh-CAT mRNA expression showed a relationship with the production of ROS. These results suggest that Hdh-CAT might play a role in stress physiology, innate immunity, testicular development, metamorphosis, and sperm cryo-tolerance of Pacific abalone.

Role of extremophilic Bacillus cereus KH1 and its lipopeptide in treatment of organic pollutant in wastewater.

An effective biosurfactant producer and extremophiles bacteria, Bacillus cereus KH1, was isolated from textile effluent and the biosurfactant was produced using molasses as the sole carbon source. Growth parameters such as pH, temperature, salinity and concentration of molasses were optimised for decolourising the textile effluent with 24-h incubation. The biosurfactant property of B. cereus KH1 was evaluated based on haemolytic activity, oil displacement technique, drop-collapsing test and emulsification index. The results of the produced biosurfactant showed a positive reaction in haemolytic activity, oil displacement technique, drop-collapsing test and exhibiting a 67% emulsification index. The cell-free broth was stable in 40 °C pH 7, 7% salinity and 7% molasses. Thin-Layer Chromatography and Fourier Transform Infrared Spectroscopy analysis revealed that the biosurfactant was a lipopeptide with a yield 2.98 g L-1. These findings proved the synergistic action of B. cereus KH1 with lipopeptide biosurfactant may accelerated the decolourisation efficiency to 87%.

Facile Preparation of a Bispherical Silver-Carbon Photocatalyst and Its Enhanced Degradation Efficiency of Methylene Blue, Rhodamine B, and Methyl Orange under UV Light.

The combination of organic and inorganic materials is attracting attention as a photocatalyst that promotes the decomposition of organic dyes. A facile thermal procedure has been proposed to produce spherical silver nanoparticles (AgNPs), carbon nanospheres (CNSs), and a bispherical AgNP-CNS nanocomposite. The AgNPs and CNSs were each synthesized from silver acetate and glucose via single- and two-step annealing processes under sealed conditions, respectively. The AgNP-CNS nanocomposite was synthesized by the thermolysis of a mixture of silver acetate and a mesophase, where the mesophase was formed by annealing glucose in a sealed vessel at 190 °C. The physicochemical features of the as-prepared nanoparticles and composite were evaluated using several analytical techniques, revealing (i) increased light absorption, (ii) a reduced bandgap, (iii) the presence of chemical interfacial heterojunctions, (iv) an increased specific surface area, and (v) favorable band-edge positions of the AgNP-CNS nanocomposite compared with those of the individual AgNP and CNS components. These characteristics led to the excellent photocatalytic efficacy of the AgNP-CNS nanocomposite for the decomposition of three pollutant dyes under ultraviolet (UV) radiation. In the AgNP-CNS nanocomposite, the light absorption and UV utilization capacity increased at more active sites. In addition, effective electron-hole separation at the heterojunction between the AgNPs and CNSs was possible under favorable band-edge conditions, resulting in the creation of reactive oxygen species. The decomposition rates of methylene blue were 95.2, 80.2, and 73.2% after 60 min in the presence of the AgNP-CNS nanocomposite, AgNPs, and CNSs, respectively. We also evaluated the photocatalytic degradation efficiency at various pH values and loadings (catalysts and dyes) with the AgNP-CNS nanocomposite. The AgNP-CNS nanocomposite was structurally rigid, resulting in 93.2% degradation of MB after five cycles of photocatalytic degradation.

Characterization and Expression Analysis of Mollusk-like Growth Factor: A Secreted Protein Involved in Pacific Abalone Embryonic and Larval Development.

Growth factors are mostly secreted proteins that play key roles in an organism's biophysical processes through binding to specific receptors on the cell surface. The mollusk-like growth factor (MLGF) is a novel cell signaling protein in the adenosine deaminase-related growth factor (ADGF) subfamily. In this study, the MLGF gene was cloned and characterized from the digestive gland tissue of Pacific abalone and designated as Hdh-MLGF. The transcribed full-length sequence of Hdh-MLGF was 1829 bp long with a 1566 bp open reading frame (ORF) encoding 521 amino acids. The deduced amino acid sequence contained a putative signal peptide and two conserved adenosine deaminase domains responsible for regulating molecular function. Fluorescence in situ hybridization localized Hdh-MLGF in the submucosa layer of digestive tubules in the digestive gland. The mRNA expression analysis indicated that Hdh-MLGF expression was restricted to the digestive gland in the adult Pacific abalone. However, Hdh-MLGF mRNA expressions were observed in all stages of embryonic and larval development, suggesting Hdh-MLGF might be involved in the Pacific abalone embryonic and larval development. This is the first study describing Hdh-MLGF and its involvement in the Pacific abalone embryonic and larval development.

A Bibliometric Analysis of Low-Cost Piezoelectric Micro-Energy Harvesting Systems from Ambient Energy Sources: Current Trends, Issues and Suggestions.

The scientific interest in piezoelectric micro-energy harvesting (PMEH) has been fast-growing, demonstrating that the field has made a major improvement in the long-term evolution of alternative energy sources. Although various research works have been performed and published over the years, only a few attempts have been made to examine the research's influence in this field. Therefore, this paper presents a bibliometric study into low-cost PMEH from ambient energy sources within the years 2010-2021, outlining current research trends, analytical assessment, novel insights, impacts, challenges and recommendations. The major goal of this paper is to provide a bibliometric evaluation that is based on the top-cited 100 articles employing the Scopus databases, information and refined keyword searches. This study analyses various key aspects, including PMEH emerging applications, authors' contributions, collaboration, research classification, keywords analysis, country's networks and state-of-the-art research areas. Moreover, several issues and concerns regarding PMEH are identified to determine the existing constraints and research gaps, such as technical, modeling, economics, power quality and environment. The paper also provides guidelines and suggestions for the development and enhancement of future PMEH towards improving energy efficiency, topologies, design, operational performance and capabilities. The in-depth information, critical discussion and analysis of this bibliometric study are expected to contribute to the advancement of the sustainable pathway for PMEH research.

Micro Energy Storage Systems in Energy Harvesting Applications: Analytical Evaluation towards Future Research Improvement.

During the last decade, countless advancements have been made in the field of micro-energy storage systems (MESS) and ambient energy harvesting (EH) shows great potential for research and future improvement. A detailed historical overview with analysis, in the research area of MESS as a form of ambient EH, is presented in this study. The top-cited articles in the field of MESS ambient EH were selected from the Scopus database, and based on articles published from 2010 to 2021, and the number of citations. The search for these top-cited articles was conducted in the third week of December 2021. Mostly the manuscripts were technical and contained an experimental setup with algorithm development (65%), whereas 27.23% of the articles were survey-based. One important observation was that the top 20 selected articles, which are the most-cited articles in the different journals, come from numerous countries of origin. This study revealed that the MESS integrated renewable energy sources (RESs) are an enhancement field of research for EH applications. On the basis of this survey, we hope to identify and solve research problems in the field of MESS and RESs integration, and provide suggestions for future developments for EH applications.

Synergistic use of siderophores and weak organic ligands during zinc transport in the rhizosphere controlled by pH and ion strength gradients.

Citrate (Cit) and Deferoxamine B (DFOB) are two important organic ligands coexisting in soils with distinct different affinities for metal ions. It has been theorized that siderophores and weak organic ligands play a synergistic role during the transport of micronutrients in the rhizosphere, but the geochemical controls of this process remain unknown. Here we test the hypothesis that gradients in pH and ion strength regulate and enable the cooperation. To this end, first we use potentiometric titrations to identify the dominant Zn(II)-Cit and Zn(II)-DFOB complexes and to determine their ionic strength dependent stability constants between 0 and 1 mol dm-3. We parametrise the Extended Debye-Hückel (EDH) equation and determine accurate intrinsic association constants (logß0) for the formation of the complexes present. The speciation model developed confirms the presence of [Zn(Cit)]-, [Zn(HCit)], [Zn2(Cit)2(OH)2]4-, and [Zn(Cit)2]4-, with [Zn(Cit)]- and [Zn2(Cit)2(OH)2]4- the dominant species in the pH range relevant to rhizosphere. We propose the existence of a new [Zn(Cit)(OH)3]4- complex above pH 10. We also verify the existence of two hexadentate Zn(II)-DFOB species, i.e., [Zn(DFOB)]- and [Zn(HDFOB)], and of one tetradentate species [Zn(H2DFOB)]+. Second, we identify the pH and ionic strength dependent ligand exchange points (LEP) of Zn with citrate and DFOB and the stability windows for Zn(II)-Cit and Zn(II)-DFOB complexes in NaCl and rice soil solutions. We find that the LEPs fall within the pH and ionic strength gradients expected in rhizospheres and that the stability windows for Zn(II)-citrate and Zn(II)-DFOB, i.e., low and high affinity ligands, can be distinctly set off. This suggests that pH and ion strength gradients allow for Zn(II) complexes with citrate and DFOB to dominate in different parts of the rhizosphere and this explains why mixtures of low and high affinity ligands increase leaching of micronutrients in soils. Speciation models of soil solutions using newly determined association constants demonstrate that the presence of dissolved organic matter and inorganic ligands (i.e., bicarbonate, phosphate, sulphate, or chlorides) do neither affect the position of the LEP nor the width of the stability windows significantly. In conclusion, we demonstrate that cooperative and synergistic ligand interaction between low and high affinity ligands is a valid mechanism for controlling zinc transport in the rhizosphere and possibly in other environmental reservoirs such as in the phycosphere. Multiple production of weak and strong ligands is therefore a valid strategy of plants and other soil organisms to improve access to micronutrients.

An Aggregated Data Integration Approach to the Web and Cloud Platforms through a Modular REST-Based OPC UA Middleware.

The Internet of Things (IoT) empowers the development of heterogeneous systems for various application domains using embedded devices and diverse data transmission protocols. Collaborative integration of these systems in the industrial domain leads to incompatibility and interoperability at different automation levels, requiring unified coordination to exchange information efficiently. The hardware specifications of these devices are resource-constrained, limiting their performance in resource allocation, data management, and remote process supervision. Hence, unlocking network capabilities with other domains such as cloud and web services is required. This study proposed a platform-independent middleware module incorporating the Open Platform Communication Unified Architecture (OPC UA) and Representational State Transfer (REST) paradigms. The object-oriented structure of this middleware allows information contextualization to address interoperability issues and offers aggregated data integration with other domains. RESTful web and cloud platforms were implemented to collect this middleware data, provide remote application support, and enable aggregated resource allocation in a database server. Several performance assessments were conducted on the developed system deployed in Raspberry Pi and Intel NUC PC, which showed acceptable platform resource utilization regarding CPU, bandwidth, and power consumption, with low service, update, and response time requirements. This integrated approach demonstrates an excellent cost-effective prospect for interoperable Machine-to-Machine (M2M) communication, enables remote process supervision, and offers aggregated bulk data management with wider domains.

Photocatalytic VOCs Degradation Efficiency of Polypropylene Membranes by Incorporation of TiO2 Nanoparticles.

A class of serious environmental contaminants related to air, namely volatile organic compounds (VOCs), has currently attracted global attention. The present study aims to remove harmful VOCs using as-prepared polypropylene membrane + TiO2 nanoparticles (PPM + TiO2 NPs) via the photocatalytic gas bag A method under UV light irradiation. Here, formaldehyde was used as the target VOC. The PPM + TiO2 NPs material was systematically characterized using various microscopic and spectroscopic techniques, including field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, X-ray diffraction, Fourier-transform infrared spectroscopy, ultraviolet-visible diffuse reflectance spectroscopy, photoluminescence spectroscopy, and contact angle measurements. These results confirm the successful preparation of PPM + TiO2 NPs, which can be applied to the degradation of VOCs. Photocatalytic degradation of formaldehyde gas reached 70% within 1 h of UV illumination. The energy bandgap and photoluminescence intensity reductions are responsible for the improved photocatalytic activity. These characteristics increase the charge transport while decreasing the recombination of electron-hole pairs.

Molecular Characterization of Tropomyosin and Its Potential Involvement in Muscle Contraction in Pacific Abalone.

Tropomyosin (TPM) is a contractile protein responsible for muscle contraction through its actin-binding activity. The complete sequence of TPM in Haliotis discus hannai (Hdh-TPM) was 2160 bp, encoding 284 amino acids, and contained a TPM signature motif and a TPM domain. Gene ontology (GO) analysis based on the amino acid sequence predicted Hdh-TPM to have an actin-binding function in the cytoskeleton. The 3D analysis predicted the Hdh-TPM to have a coiled-coil α-helical structure. Phylogenetically, Hdh-TPM formed a cluster with other TPM/TPM1 proteins during analysis. The tissue-specific mRNA expression analysis found the higher expression of Hdh-TPM in the heart and muscles; however, during embryonic and larval development (ELD), the higher expression was found in the trochophore larvae and veliger larvae. Hdh-TPM expression was upregulated in fast-growing abalone. Increasing thermal stress over a long period decreased Hdh-TPM expression. Long-term starvation (>1 week) reduced the mRNA expression of Hdh-TPM in muscle; however, the mRNA expression of Hdh-TPM was significantly higher in the mantle, which may indicate overexpression. This study is the first comprehensive study to characterize the Hdh-TPM gene in Pacific abalone and to report the expression of Hdh-TPM in different organs, and during ELD, different growth patterns, thermal stress, seasonal changes, and starvation.

Review on Comparison of Different Energy Storage Technologies Used in Micro-Energy Harvesting, WSNs, Low-Cost Microelectronic Devices: Challenges and Recommendations.

This paper reviews energy storage systems, in general, and for specific applications in low-cost micro-energy harvesting (MEH) systems, low-cost microelectronic devices, and wireless sensor networks (WSNs). With the development of electronic gadgets, low-cost microelectronic devices and WSNs, the need for an efficient, light and reliable energy storage device is increased. The current energy storage systems (ESS) have the disadvantages of self-discharging, energy density, life cycles, and cost. The ambient energy resources are the best option as an energy source, but the main challenge in harvesting energy from ambient sources is the instability of the source of energy. Due to the explosion of lithium batteries in many cases, and the pros associated with them, the design of an efficient device, which is more reliable and efficient than conventional batteries, is important. This review paper focused on the issues of the reliability and performance of electrical ESS, and, especially, discussed the technical challenges and suggested solutions for ESS (batteries, supercapacitors, and for a hybrid combination of supercapacitors and batteries) in detail. Nowadays, the main market of batteries is WSNs, but in the last decade, the world's attention has turned toward supercapacitors as a good alternative of batteries. The main advantages of supercapacitors are their light weight, volume, greater life cycle, turbo charging/discharging, high energy density and power density, low cost, easy maintenance, and no pollution. This study reviews supercapacitors as a better alternative of batteries in low-cost electronic devices, WSNs, and MEH systems.

Selective growth of Ti3+/TiO2/CNT and Ti3+/TiO2/C nanocomposite for enhanced visible-light utilization to degrade organic pollutants by lowering TiO2-bandgap.

A convenient route was developed for the selective preparation of two stable nanocomposites, Ti3+/TiO2/CNT (labeled as TTOC-1 and TTOC-3) and Ti3+/TiO2/carbon layer (labeled as TTOC-2), from the same precursor by varying the amount of single-walled carbon nanotubes used in the synthesis. TiO2 is an effective photocatalyst; however, its wide bandgap limits its usefulness to the UV region. As a solution to this problem, our prepared nanocomposites exhibit a small bandgap and wide visible-light (VL) absorption because of the introduction of carbonaceous species and Ti3+ vacancies. The photocatalytic efficiency of the nanocomposites was examined via the degradation of methylene blue dye under VL. Excellent photocatalytic activity of 83%, 98%, and 93% was observed for TTOC-1, TTOC-2, and TTOC-3 nanocomposites within 25 min. In addition, the photocatalytic degradation efficiency of TTOC-2 toward methyl orange, phenol, rhodamine B, and congo red was 28%, 69%, 71%, and 91%, respectively, under similar experimental conditions after 25 min. Higher reusability and structural integrity of the as-synthesized photocatalyst were confirmed within five consecutive runs by photocatalytic test and X-ray diffraction analysis, respectively. The resulting nanocomposites provide new insights into the development of VL-active and stable photocatalysts with high efficiencies.

Hierarchical Nanocauliflower Chemical Assembly Composed of Copper Oxide and Single-Walled Carbon Nanotubes for Enhanced Photocatalytic Dye Degradation.

We present the fabrication and proficient photocatalytic performance of a series of heterojunction nanocomposites with cauliflower-like architecture synthesized from copper(II) oxide (CuO) nanocrystals and carbon nanotubes with single walls (SWCNTs). These unique photocatalysts were constructed via simplistic recrystallization succeeded by calcination and were labeled as CuOSC-1, CuOSC-2, and CuOSC-3 (representing the components; CuO and SC for SWCNTs, and the calcination time in hours). The photocatalytic potency of the fabricated nanocomposites was investigated on the basis of their capability to decompose methylene blue (MB) dye under visible-light irradiation. Every as-synthesized nanocomposite was effective photocatalyst for the photodecomposition of an MB solution. Moreover, CuOSC-3 exhibited the best photocatalytic activity, with 96% degradation of the visible-light irradiated MB solution in 2 h. Pure CuO nanocrystals generated through the same route and pure SWCNTs were used as controls, where the photocatalytic actions of the nanocomposite samples were found to be remarkably better than that of either the pure CuO or the pure SWCNTs. The recycling proficiency of the photocatalysts was also explored; the results disclosed that the samples could be applied for five cycles without exhibiting a notable change in photocatalytic performance or morphology.