Biomapping of Microbial Indicators on Beef Subprimals Subjected to Spray or Dry Chilling over Prolonged Refrigerated Storage.

As the global meat market moves to never frozen alternatives, meat processors seek opportunities for increasing the shelf life of fresh meats by combinations of proper cold chain management, barrier technologies, and antimicrobial interventions. The objective of this study was to determine the impact of spray and dry chilling combined with hot water carcass treatments on the levels of microbial indicator organisms during the long-term refrigerated storage of beef cuts. Samples were taken using EZ-Reach™ sponge samplers with 25 mL buffered peptone water over a 100 cm2 area of the striploin. Sample collection was conducted before the hot carcass wash, after wash, and after the 24 h carcass chilling. Chilled striploins were cut into four sections, individually vacuum packaged, and stored to be sampled at 0, 45, 70, and 135 days (n = 200) of refrigerated storage and distribution. Aerobic plate counts, enterobacteria, Escherichia coli, coliforms, and psychrotroph counts were evaluated for each sample. Not enough evidence (p > 0.05) was found indicating the hot water wash intervention reduced bacterial concentration on the carcass surface. E. coli was below detection limits (<0.25 CFU/cm2) in most of the samples taken. No significant difference (p > 0.05) was found between coliform counts throughout the sampling dates. Feed type did not seem to influence the (p > 0.25) microbial load of the treatments. Even though no immediate effect was seen when comparing spray or dry chilling of the samples at day 0, as the product aged, a significantly lower (p < 0.05) concentration of aerobic and psychrotrophic organisms in dry-chilled samples could be observed when compared to their spray-chilled counterparts. Data collected can be used to select alternative chilling systems to maximize shelf life in vacuum packaged beef kept over prolonged storage periods.

Presence of Salmonella and Escherichia coli O157 on the hide, and presence of Salmonella, Escherichia coli O157 and Campylobacter in feces from small-ruminant (goat and lamb) samples collected in the United States, Bahamas and Mexico.

The objective of this study was to evaluate the frequency of Salmonella and E. coli O157 found on the hides, as well as presence of Salmonella, Campylobacter and E. coli O157 found in small-ruminant fecal samples from abattoirs and farms in California, New Mexico, Texas, Mexico and the Bahamas. In small-ruminant fecal samples, overall organism presence was identified as 13.9% Salmonella (n=532), 15.3% E. coli O157 (n=477) and 80.7% Campylobacter (n=176). Overall, on small-ruminant hide surfaces, Salmonella frequency was 17.1% (N=339) and E. coli O157 was detected at 1.5% (n=266). The overall lower detection (P<0.0001) of E. coli O157 from hide samples (1.5%) when compared to presence in fecal samples (15.3%), is not consistent with trends expected. Results from this study can be used to better understand and potentially control pathogens in small-ruminants utilized for meat and milk.

Salmonella Presence in Mandibular, Mesenteric, and Subiliac Lymph Nodes Collected from Sheep and Goats in the United States.

Even though often underappreciated in the United States, meat derived from goats and lambs is a valuable global commodity. Although extensive studies have been conducted examining pathogen prevalence in beef, pork, and poultry species, less research is available about pathogen presence in small ruminants. Understanding the presence of Salmonella in small ruminants can influence processing method improvements for these species to minimize food safety risks. The objective of this study was to establish the Salmonella presence in lymph nodes from sheep (n = 311) and goats (n =357) in the United States. Mandibular, mesenteric, and subiliac lymph nodes were collected from animals at abattoirs located in California, New Mexico, and Texas over a 14-month period. Lymph nodes were analyzed for Salmonella presence by using standard isolation methods, and presumptive isolates were confirmed with latex agglutination. Salmonella was detected in 3.15% of mandibular lymph nodes (n = 222), 5.83% of mesenteric lymph nodes (n = 223), and 7.62% of subiliac lymph nodes (n = 223). A total of 30 animals (13.39%) were found to have one or more Salmonella -positive lymph nodes. Our data indicate Salmonella is harbored within the lymph nodes of small ruminants. With this knowledge, further work can target strategies and interventions to minimize the risk associated with this pathogen during the processing of small ruminants.