The "Traditional Chinese medicine regulating liver regeneration" treatment plan for reducing mortality of patients with hepatitis B-related liver failure based on real-world clinical data / 医学前沿

On the basis of real-world clinical data, the study aimed to explore the effect and mechanisms of the treatment plan of "traditional Chinese medicine (TCM) regulating liver regeneration." A total of 457 patients with HBV-related liver failure were retrospectively collected. The patients were divided into three groups: the modern medicine control group (MMC group), patients treated with routine medical treatment; the control group combining traditional Chinese and Western medicine (CTW), patients treated with routine medical treatment plus the common TCM formula; and the treatment group of "TCM regulating liver regeneration" (RLR), patients treated with both routine medical treatment and the special TCM formula of RLR. After 8 weeks of treatment, the mortality of patients in the RLR group (12.31%) was significantly lower than those in the MMC (50%) and CTW (29.11%) groups. Total bilirubin level significantly decreased and albumin increased in the RLR group when compared with the MMC and CTW groups (P < 0.05). In addition, there were significant differences in the expression of several cytokines related to liver regeneration in the RLR group compared with the MMC group. RLR treatment can decrease jaundice, improve liver function, and significantly reduce the mortality in patients with HBV-related liver failure. The mechanism may be related to the role of RLR treatment in influencing cytokines related to liver regeneration.

Establishment and application value of a monosodium glutamate-rat-liver regeneration model / 临床肝胆病杂志

The establishment of an animal model of liver regeneration in a state of neuro-endocrine-immune network dysfunction is an urgent need to promote the research on the regulatory mechanism of liver regeneration. The monosodium glutamate (MSG)-rat-liver regeneration model is a composite model of liver regeneration which combines the MSG-rat model with the model of partial hepatectomy (PHx) or hepatotoxicity (for example, CCl4), and it is mainly used to investigate liver regeneration and its regulatory mechanism in the state of neuro-endocrine-immune network dysfunction, mechanisms and drug screening of liver-brain syndrome, and mechanisms of disease-syndrome combination and drug efficacy. Dysregulated liver regeneration process is observed in the MSG-rat-liver regeneration model established by the MSG-rat model and PHx, which is significantly different from the liver regeneration process in normal rats after major hepatectomy. The MSG-rat-liver regeneration model established by the MSG-rat model and hepatotoxicity has a significantly higher degree of liver fibrosis than normal rats with CCl4-induced liver fibrosis. The hypothalamic-pituitary-hepatic axis is an important mechanism of the influence of the external environment on liver regeneration, and the imbalance of epithelial-mesenchymal transition/mesenchymal-epithelial transition is an important mechanism of the influence of intrahepatic microenvironment on abnormal liver regeneration (liver fibrosis). The research on MSG-rat-liver regeneration model preliminarily reveals the efficacy and mechanism of traditional Chinese medicine in the prevention and treatment of liver and related diseases (including liver-brain syndrome) by influencing the neuro-endocrine-immune-liver regeneration regulatory network and promotes the research on the combination of diseases and syndromes. The establishment and application of the MSG-rat-liver regeneration model has important scientific significance and application value in further exploration of the complex mechanism of liver regeneration and the mechanism of traditional Chinese medicine in regulating liver regeneration.

Comparison of pharmacokinetics and subacute toxicity for four arsenic species in rats / 中国食品卫生杂志

Objective Study on the absorption,distribution,transformation,excretion and toxicity of four forms of arsenic in rats.Methods Four thousand and fifty data were obtained from 28 days animal metabolism experiments of 81 rats exposed to the four As species.Distribution,metabolism,excretion,and subacute toxicity of 4 As species were compared by analyzing the arsenate [As(V)],arsenite [As(Ⅲ)],methyl arsenate (MMAV) and dimethyl arsenate (DMAV) content of rat feces (excrement and urine),blood,seven organs,and liver and kidney pathology.Results After oral administration of each As species,82.9％ of As(Ⅲ),85.1％ of As(V),95.0％ of MMAV and 96.2％ of DMAV were accumulatively secreted via feces and urine,while 16.2％ of As(Ⅲ),14.1％ of As(V),4.65％ of MMAV and 0.120％ of DMAV were detected in blood.The DMAV accumulated in blood and urine after dosing As(Ⅲ),As(V) and MMAV,and the content of DMAV in the blood was 8 times greater than urine.Four kinds of arsenic had effects on rat liver and kidney function,the influence on liver was acute phase effect while that on renal was cumulative effects.The effect of As(Ⅲ) was the most significant,followed by MMAV.Four kinds of arsenic could cause tissue deformation and inflammatory cells infiltration,but showed no difference between groups.Conclusion The distribution,metabolism,excretion,and toxicity of the four kinds of arsenic in rats were different in varying degrees.The research of health risk for arsenic should consider its different forms.

Research hotspots of DRGs based on co-word cluster analysis / 中华医院管理杂志

Objective To reveal the research hotspots and development trend of DRGs based on literature, and to provide a basis for domestic scholars and hospitals .Methods Literature review was made in database CNKI and Wanfang database , using DRGs and its co-words ( both Chinese and English ) to retrieve literatures published before April 2017 , and describing such distribution as paper publication year , journal, and first author.Co-word cluster analysis was then performed on the co-occurrence matrix of high frequency keywords to reveal the research hotspots and development trends in line with the subject strategic diagram.Results 2594paperswereretrievedand1303ofthemwereretainedwiththoseredundantand irrelevant bibliographies excluded .Observation of the distribution of the year , journal and first author revealed that papers in DRGs field were in rapid growth , while the journals and first authors were distributed evenly.These literature focused on eight research hotspots , namely medical insurance payment system , medical quality and efficiency, hospital management, DRGs payment, case-mix, coding uniformity, medical records management , and evaluation system .In the subject strategic diagram , medical insurance payment system, medical quality and efficiency were placed in the fourth quadrant , the DRGs payment , case-mix, coding uniformity in the first quadrant , hotspot coding uniformity in the second , and the last two hotspots were in the third .Conclusions DRGs has attracted attention of many scholars and institutions in China , as evidenced by numerous articles published in various journals .These articles covered a wide range of topics , and were in different development stages .Researchers should keep an eye on progress of mature themes , and promote practical exploration of immature topics .

Prevention and treatment of liver fibrosis by regulating liver regeneration / 临床肝胆病杂志

Liver fibrosis is regarded as a form or status of liver regeneration disorder. Therefore, from a new perspective of regulating liver regeneration, we put forward a new strategy for the prevention and treatment of liver fibrosis. This strategy not only focuses on confrontational reverse regulation, but also emphasizes positive induction. Moreover, this strategy applies an integrated method of promotion and inhibition, which improves liver injury and regeneration and corrects the abnormal liver regeneration and the normal liver regeneration disorder. Furthermore, this strategy also advocates the combination of prevention and treatment, which prevents the occurrence of liver fibrosis or a disorder of liver regeneration and treats the rapid progression of liver fibrosis. The imbalance between epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) is involved in the CCl4-induced liver fibrosis in rats. Diwu Yanggan capsules can delay the progression of liver fibrosis and attenuate the degree of fibrosis, which is associated with the regulation of liver regeneration by modulating the imbalance between EMT and MET (inhibiting EMT and/or promoting MET).

"Fundamental and Clinical Application for Liver Disease Treatment with Therapeutic Principle of""Tonifying the Kidney to Promote Liver Regeneration and Repair""" / 世界科学技术-中医药现代化

This paper was aimed to expound the concept , theoretical foundation , theoretical system as well as scientific connotation of the new therapeutic principle of tonifying the kidney to promote liver regeneration and repair . a brief introduction was provided for the development in both fundamental research and clinical appli-cations of liver disease treatment . It provided theoretical references for the clinical treatment of liver diseases .

"Objective and Quantitative Study on the Syndrome of ""Liver-Kidney Essence Deficiency"" in Chronic Liver Diseases" / 世界科学技术-中医药现代化

This study was aimed to establish an objective and quantitative index system and standards of diag-nosis and treatment on the syndrome of liver-kidney essence deficiency for chronic liver diseases , in order to improve the level of diagnosis and treatment , clinical efficacy and service capabilities in the prevention and treatment of chronic liver diseases in traditional Chinese medicine ( TCM ) . Modern biological indicators of liver damage and liver regeneration were used as objective and quantitative indicators to study the syndrome of liv-er-kidney essence deficiency . And the syndrome of liver-kidney essence deficiency , which was used to be considered as a recessive syndrome, was finally turning into a dominant syndrome . Hence , the symptom i-dentification can be accurate . The establishment of an objective and quantitative index system and standards of diagnosis and treatment of liver-kidney essence deficiency , which was based on biological indicators such as indicators of histological damage in liver , indicators of hypohepatia , indicators of aregeneratory and indicators of liver dysfunction . According to all indicators , a dynamic monitoring was given on the development law of the syndrome of liver-kidney essence deficiency . All indicators provided references for the prognosis determina-tion and syndrome differentiation . It was concluded that the objective and quantitative research on the syn-drome of liver-kidney essence deficiency in chronic liver diseases is an effective way to improve the level of diagnosis and treatment, clinical efficacy and service capabilities in the prevention and treatment of chronic liver diseases in TCM , which is worth further study .

Effects of Zuogui Pill on Gene Expression Profile of Regenerate Hepatic Tissue in MSG-Liver Regeneration-Rat / 中华中医药杂志

Objective: To research the effects of Zuogui Pill on the gene expression profile of regenerate hepatic tissue in MSG-liver regeneration-rat.Methods: MSG-liver regeneration-rat models were copied;therapy group were given Zuogui Pill(5g/kg) by intragastric administration.Rats were executed at the 5th day after operation,and the regenerate liver tissues were frozen by liquid nitrogen.1176 gene chips of gene expression profile correlated to cell differentiation and multiplication were adopted to observe the changes of gene expression profile in the regenerate liver tissues.Results: There were 292 differential expression genes in therapy group compared with model group(20 genes were up-regulation expressed and 272 genes were down regulation expressed).Conclusion: Zuogui Pill has significant effects on the gene expression profile of regenerate hepatic tissue in MSG-liver regeneration-rat,and most of the genes are down regulation expressed.The result hints that Zuogui Pill could influence the liver regeneration by regulating the gene expressions of MSG-live regeneration-rat.Down regulation of the gene expressions of regenerate hepatic tissue in MSG-liver regeneration-rat possibly is one of the important molecular mechanisms of Zuogui Pill in nourishing liver and kidney.