The correlation between hEST2 reverse transcription motif and the activity of telomerase / 中国癌症杂志

Purpose:Telomerase was discovered recently as an important tumor marker, and the necessary gene sequence which is closely related with telomerase activity is one of the methods to study the telomerase regulation mechanism. Methods:RT PCR, Northern blot were used to test the relationship between the expression of hEST2 reverse transcriptase motif fragment and telomerase activity, classic telomerase measurement method was set up as contol. Results:Indicate that the expression of 7 RT motifs fragment which include telomerase specific T motif have a close relationship with telomerase activity. Conclusions:It will be helpful to further research of telomerase regulation mechanism, diagnosis and inhibition.