1.
Proc Natl Acad Sci U S A
; 90(17): 8118-22, 1993 Sep 01.
Artigo
em Inglês
| MEDLINE
| ID: mdl-8367472
RESUMO
Recombination walking is based on the genetic selection of specific human clones from a yeast artificial chromosome (YAC) library by homologous recombination. The desired clone is selected from a pooled (unordered) YAC library, eliminating labor-intensive steps typically used in organizing and maintaining ordered YAC libraries. Recombination walking represents an efficient approach to library screening and is well suited for chromosome-walking approaches to the isolation of genes associated with common diseases.