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1.
Materials (Basel) ; 16(12)2023 Jun 19.
Artigo em Inglês | MEDLINE | ID: mdl-37374642

RESUMO

This study aimed to assess the feasibility of utilizing geopolymer for repairing reinforced concrete beams. Three types of beam specimens were fabricated: benchmark specimens without any grooves, rectangular-grooved beams, and square-grooved beams. The repair materials employed included geopolymer material, and epoxy resin mortar, while carbon fiber sheets were used as reinforcement in select cases. The repair materials were applied to the rectangular and square-grooved specimens, with the carbon fiber sheets attached to the tension side of the specimens. To evaluate the flexural strength of the concrete specimens, a third-point loading test was conducted. The test results indicated that the geopolymer exhibited higher compressive strength and shrinkage rate compared to the epoxy resin mortar. Furthermore, the specimens reinforced with carbon fiber sheets demonstrated even greater strength than the benchmark specimens. In terms of flexural strength under cyclic third-point loading tests, the carbon fiber-reinforced specimens exhibited the ability to withstand over 200 cycles of repeated loading at 0.8 times the ultimate load. In contrast, the benchmark specimens could only withstand seven cycles. These findings highlight that the use of carbon fiber sheets not only enhances compressive strength but also improves resistance to cyclic loading.

2.
PeerJ ; 10: e12921, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35321406

RESUMO

Drought acts as a major abiotic stress that hinders plant growth and crop productivity. It is critical, as such, to discern the molecular response of plants to drought in order to enhance agricultural yields under droughts as they occur with increasing frequency. Pear trees are among the most crucial deciduous fruit trees worldwide, and yet the molecular mechanisms of drought tolerance in field-grown pear remain unclear. In this study, we analyzed the differences in transcriptome profiles of pear leaves, branches, and young fruits in irrigation vs field-drought conditions over the growing seasons. In total, 819 differentially expressed genes (DEGs) controlling drought response were identified, among which 427 DEGs were upregulated and 392 DEGs were downregulated. Drought responsive genes were enriched significantly in monoterpenoid biosynthesis, flavonoid biosynthesis, and diterpenoid biosynthesis. Fourteen phenylpropanoid, five flavonoid, and four monoterpenoid structural genes were modulated by field drought stress, thereby indicating the transcriptional regulation of these metabolic pathways in fruit exposed to drought. A total of 4,438 transcription factors (TFs) belonging to 30 TF families were differentially expressed between drought and irrigation, and such findings signal valuable information on transcriptome changes in response to drought. Our study revealed that pear trees react to drought by modulating several secondary metabolic pathways, particularly by stimulating the production of phenylpropanoids as well as volatile organic compounds like monoterpenes. Our findings are of practical importance for agricultural breeding programs, while the resulting data is a resource for improving drought tolerance through genetic engineering of non-model, but economically important, perennial plants.


Assuntos
Pyrus , Transcriptoma , Humanos , Transcriptoma/genética , Pyrus/genética , Secas , Melhoramento Vegetal , Redes e Vias Metabólicas/genética , Monoterpenos/metabolismo
3.
PLoS One ; 16(2): e0246070, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33545712

RESUMO

Pear is one of the most important economic fruits worldwide. The productivity is often negatively affected by drought disaster, but the effects and adaptive mechanism of pear in response to drought stress has not been well understood at the gene transcription levels. Using Illumina HiSeq 2500, the transcriptome from 'Yulu Xiang' Pear leaves were sequenced and analyzed to evaluate the effects of long-term drought stress on the expression of genes in different biosynthetic pathways. Results showed that long-term drought stress weakened antioxidant systematization and impaired the synthesis of photosynthetic pigment in 'Yulu Xiang' Pear leaves. The reduced light utilization and photosynthetic productivity finally resulted in the inhibited fruit development. The transcriptome survey and expression analysis identified 2,207 differentially expressed genes (DEGs) which were summarized into the 30 main functional categories. DEGs analysis showed that the enzyme genes involved in phenylpropanoid biosynthesis under drought stress were up-regulated, and the promoted process of phenylpropanoid synthesis may be beneficial to reduce the transpiration rate and increase water use efficiency of 'Yulu Xiang' Pear leaves. Up-regulated malate dehydrogenase expression were also observed in drought stress groups, and the activated soluble sugar biosynthesis could be helpful to promote osmotic regulation and increase antioxidant capacity to enhance drought resistance of leaves. The mRNA expression of enzyme genes associated with hormones including ethylene, abscisic acid, and gibberellin were higher in drought stress groups than that in control, indicating a promoted cell proliferation under drought stress. Long-term drought stress significantly decreased photosynthetic productivity, and negatively affected development of 'Yulu Xiang' Pear. Transcriptome survey and expression analysis reveals that the inhibited photosynthesis could be closely related with drought-induced lignification and hormones synthesis, and the present dataset can provide more valuable information to analyze the function of drought stress-related genes improving plant drought tolerance.


Assuntos
Adaptação Fisiológica/genética , Secas , Perfilação da Expressão Gênica , Pyrus/genética , Pyrus/fisiologia , Estresse Fisiológico/genética , Inquéritos e Questionários
4.
Micromachines (Basel) ; 10(2)2019 Jan 25.
Artigo em Inglês | MEDLINE | ID: mdl-30691047

RESUMO

This paper describes the design of a front-end receiver amplifier for capacitive micromachined ultrasonic transducer (CMUT). The proposed operational amplifier (op amp) consists of a full differential folded-cascode amplifier stage followed by a class AB output stage. A feedback resistor is applied between the input and the output of the op amp to make a transimpedance amplifier. We analyzed the equivalent circuit model of the CMUT element operating in the receiving mode and obtained the static output impedance and center frequency characteristics of the CMUT. The op amp gain, bandwidth, noise, and power consumption trade-offs are discussed in detail. The amplifier was fabricated using GlobalFoundries 0.18-µm complementary metal-oxide-semiconductor (CMOS) technology. The open loop gain of the amplifier is approximately 65 dB, and its gain bandwidth product is approximately 29.5 MHz. The measured input reference noise current was 56 nA/√Hz@3 MHz. The amplifier chip area is 325 µm × 150 µm and the op amp is powered by 3.3 V, the static power consumption is 11 mW. We verified the correct operation of our amplifier with CMUT and echo-pulse shown that the CMUT center frequency is 3 MHz with 92% fractional bandwidth.

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