The significance of Siglec-15 expression in resectable non-small cell lung cancer.

Siglec-15 (S15) is another important mechanism of tumor immune escape besides the PD-L1/PD-1 pathway and represents a new kind of immune checkpoint inhibitor. However, the associations of tumor Siglec-15 expression with clinicopathological characteristics and outcomes of non-small cell lung cancer (NSCLC), and tumor-infiltrating lymphocytes (TILs) in a tumor microenvironment (TME) have so far been unclear. A total of 324 NSCLC surgical samples on tumor microarray were used in this study for investigating the association of S15 expression with clinicopathological characteristics and overall survival (OS) as well as correlation with TILs using multiplex immunofluorescence staining and PD-L1. Results showed that the expression of S15 in adenocarcinoma was significantly higher than that in squamous cell carcinoma. S15 expression was positively correlated with CD8+ T cell density in the stroma. The expression rate of PD-L1 in lung squamous cell carcinoma was higher than that in lung adenocarcinoma. S15 expression was not associated with the prognosis of early NSCLC. The pathological mechanism of the co-expression of S15 and PD-L1 in resectable NSCLC remains to be further studied.

[Role of overexpression of myeloid cell leukemia-1-long in prognosis of malignant pleomorphic adenoma].

Objective: To evaluate the clinicopathological significance of the expression of the two myeloid cell leukemia-1 isoforms, myeloid cell leukemia-1-long (Mcl-1L) and myeloid cell leukemia-1-short (Mcl-1S) in malignant pleomorphic adenoma (MPA). Methods: A total of 56 patients with MPA treated with surgery, the expression of Mcl-1L and Mcl-1S were detected by quantitative real-time PCR (qPCR) in mRNA level, and by Western blotting in protein level, respectively. The correlation between clinicopathological parameters (gender, age, site, habits, size, node, metastasis, recurrence) was done using the chi-square test. Kaplan-Merier curve was used to analyze the survival in all patients, Log Rank test was performed to observe the difference between groups. Cox's proportional hazard test was used to identify the factors that were independent predictors of survival. Results: qPCR showed overexpression of Mcl-1L in 66.1%MPA patients (37/56), with significant difference in terms of tumor site, significantly higher expression of Mcl-1L in MPA (parotid gland: 2.21±1.07, sublingual gland: 2.12±1.08, other sites: 1.83±0.81) than benign pleomorphic adenoma tissue (1.00±0.19) (P<0.01). Western blotting analysis showed significantly higher expression of Mcl-1L in MPA (parotid gland: 1.02±0.43, sublingual gland: 0.71±0.29, other sites: 0.70±0.30) than benign pleomorphic adenoma tissue (0.39±0.08) (P<0.01). In malignant pleomorphic adenoma patients, high Mcl-1L expression was significantly associated with node positivity (P=0.045), advanced tumor size (P=0.002). Kaplan-Meier survival line analysis indicated a significantly lower survival time in Mcl-1L higher-expression patients (24.79±9.71) than lower-expression patients (30.59±9.01) (P=0.017). Multivariate analysis indicated Mcl-1L to be an independent prognostic factor for MPA (P=0.002). Conclusions: The studies suggest that overexpression of Mcl-1L is associated with poor prognosis in MPA. Mcl-1L is an independent prognostic factor in malignant pleomorphic adenoma.

Selecting the optimum conditions for two-dimensional difference gel electrophoresis of proteins expressed in Populus euphratica.

We selected the optimum conditions for two-dimensional difference gel electrophoresis (2D-DIGE) of proteins expressed in the heteromorphic leaves of Populus euphratica Oliv. by adjusting the isoelectric focusing, the loading quantity, the concentration of the electrophoretic gel, and other parameters. The results of our study showed that protein separation was improved with many clear protein spots observed, and the differentiations were obvious. The findings of this study will be useful for future studies of protein expression in the heteromorphic leaves of P. euphratica.

Thermoluminescence studies of LiSrBO(3):RE(3+) (RE=Dy, Tb, Tm and Ce).

The polycrystalline samples of LiSrBO(3):RE(3+) were synthesized by high-temperature solid-state reaction. The influence of different rare earth dopants, i.e. Dy(3+), Tb(3+), Tm(3+) and Ce(3+), on thermoluminescence (TL) of LiSrBO(3) phosphor was discussed. We also investigated some dosimetric characteristics of LiSrBO(3):Tm(3+). The effect of the concentration of Tm(3+) on the TL intensity was investigated and the result of which showed that the optimum mole fraction of Tm(3+) was 1%. The TL kinetic parameters of LiSrBO(3):0.01Tm(3+) were calculated by the computer glow curve deconvolution (CGCD) method. We also studied the three-dimensional (3D) TL emission spectrum of LiSrBO(3):0.01Tm(3+) under gamma-ray irradiation. The dose-response of LiSrBO(3):0.01Tm(3+) to gamma-ray was linear in the range from 1 to 400mGy.