Concentration properties of biopolymers via dead-end forward osmosis.

Extracellular polymeric substances (EPSs) in excess sludge of wastewater treatment plants are valuable biopolymers that can act as recovery materials. However, effectively concentrating EPSs consumes a significant amount of energy. This study employed novel energy-saving pressure-free dead-end forward osmosis (DEFO) technology to concentrate various biopolymers, including EPSs and model biopolymers [sodium alginate (SA), bovine serum albumin (BSA), and a mixture of both (denoted as BSA-SA)]. The feasibility of the DEFO technology was proven and the largest concentration ratios for these biopolymers were 94.8 % for EPSs, 97.1 % for SA, 97.8 % for BSA, and 98.4 % for BSA-SA solutions. An evaluation model was proposed, incorporating the FO membrane's water permeability coefficient and the concentrated substances' osmotic resistance, to describe biopolymers' concentration properties. Irrespective of biopolymer type, the water permeability coefficient decreased with increasing osmotic pressure, remained constant with increasing feed solution (FS) concentration, increased with increasing crossing velocity in the draw side, and showed little dependence on draw salt type. In the EPS DEFO concentration process, osmotic resistance was minimally impacted by osmotic pressure, FS concentration, and crossing velocity, and monovalent metal salts were proposed as draw solutes. The interaction between reverse diffusion metal cations and EPSs affected the structure of the concentrated substances on the FO membrane, thus changing the osmotic resistance in the DEFO process. These findings offer insights into the efficient concentration of biopolymers using DEFO.

Systemic immune-inflammation index is associated with cardiac complications following acute ischemic stroke: A retrospective single-center study.

BACKGROUND: Stroke-induced heart syndrome is a feared complication of ischemic stroke, that is commonly encountered and has a strong association with unfavorable prognosis. More research is needed to explore underlying mechanisms and inform clinical decision making. This study aims to explore the relationship between the early systemic immune-inflammation (SII) index and the cardiac complications after acute ischemic stroke. METHODS: Consecutive patients with acute ischemic stroke were prospectively collected from January 2020 to August 2022 and retrospectively analyzed. We included subjects who presented within 24 hours after symptom onset and were free of detectable infections or cancer on admission. SII index [(neutrophils × platelets/ lymphocytes)/1000] was calculated from laboratory data at admission. RESULTS: A total of 121 patients were included in our study, of which 24 (19.8 %) developed cardiac complications within 14 days following acute ischemic stroke. The SII level was found higher in patients with stroke-heart syndrome (p<.001), which was an independent predictor of stroke-heart syndrome (adjusted odds ratio 5.089, p=.002). CONCLUSION: New-onset cardiovascular complications diagnosed following a stroke are very common and are associated with early SII index.

Phosphate recovery from sludge-incinerated ash by adsorption with hydrotalcite synthesized by metals in the ash.

Selective adsorption of phosphorus (P) from the acidic leachate of sludge-incinerated ash (SIA) becomes more attractive due to avoiding removing heavy metals. Especially, layered double hydroxides (LDHs) as an anion adsorbent could be applied into this area owing to their good capacity on P-adsorption and low cost on preparation. Interestingly, SIA contains more aluminum (Al) and iron (Fe) needed to be removed prior to P-recovery, and removed Al and Fe could be utilized to synthesize LDHs, like Mg/Al-LDH and Mg/Fe-LDH. With this study, Mg/Al-LDH-r and Mg/Fe-LDH-r were economically synthesized with Al and Fe removed from SIA, which were similar in their chemical structures to commercial LDHs. The synthesized LDHs had a high P-adsorption capacity, up to 95.0%. The maximal phosphate capacity of the recovered LDHs (Mg/Al-LDH-r and Mg/Fe-LDH-r) was 239.0 and 199.8 mg P/g LDHs, respectively. "NaOH + desalinated brine" as a new desorption solution could achieve a desorption ratio at about 80%, which could reduce the liquid-solid ratio by at least 60%, greatly decreasing the desorption cost. Pot trials demonstrated that the desorbed and precipitated CaP could promote the growth of maize as well as a commercial P-fertilizer. Furthermore, the adsorbed phosphate by LDHs could be directly used as a slow-released P-fertilizer and also improve the pH value of acidic soil, completely deleting the desorption process.

Near-IR-Regulated Composite Hydrogel with Real-Time Infection Monitoring and a Combined Antibacterial Effect for Efficient Wound Management.

Chronic wounds induced by bacterial infection have seriously affected the health of people in the world. So, it is meaningful to develop a novel strategy with real-time infection monitoring and excellent antibacterial performance for enhancing wound management. Herein, we constructed a composite hydrogel by loading the pH indicator bromothymol blue (BTB) and gold nanocages containing 2,2'-azobis[2-(2-imidazolin-2-yl)propane] dihydrochloride (Au NCs@AIPH) into a polyacrylamide-co-poly(acrylic anhydride-modified oxidized sodium alginate) (PAM-co-PAOSA) hydrogel. In vitro and in vivo experimental results demonstrated that the composite hydrogel could effectively detect bacteria and diagnose the infection status of a wound in real time by showing visible color changes. In addition, the composite hydrogel containing Au NCs@AIPH possessed an excellent photothermal effect under near-IR (NIR) laser irradiation. The photothermal effect further activated AIPH to generate toxic free radicals to form combined antibacterial therapy for accelerating wound healing. Moreover, the composite hydrogel showed great biocompatibility. Therefore, the multifunctional hydrogel provided a novel wound management strategy for bacterial infection diagnosis and combined therapy in an infected wound.

Recovering and potentially applying of alginate like extracellular polymers from anaerobic digested sludge.

Extracellular polymeric substances (EPS) are biopolymers contained in both aerobic and anaerobic sludge. In EPS, alginate like extracellular polymers (ALE) is thought as a highly valued material, which have been widely studied with aerobic sludge. Nevertheless, a curiosity on ALE remains in anaerobic digested sludge (ADS). With 5 different sludge sources, anaerobic digestion of excess sludge was conducted in a batch mode, and then ADS was used to extract ALE and to analyze its physicochemical properties for potential applications. The yield of ALE extracted from ADS (ALE-ADS) ranged from 119.4 to 179.4 mg/g VSS. The compositional characteristics of ALE-ADS observed by FT-IR, 3D-EEM and UV-Vis spectroscopy revealed that there were minor differences in the composition and property of ALE-ADS but a similarity of 62 %-70 % to a commercial alginate remained in terms of chemical functional groups. Moreover, ALE-ADS composed of 1,4-linked ß-d-mannuronic acid (M) and 1,4 α-l-guluronic acid (G) residues that form blocks of GG (20.8 %-33.8 %), MG (12.8 %-30.1 %) and MM (6.6 %-15.1 %), respectively. Based on the gel-forming capacity, film-forming property, adsorbility, and amphiphilicity, ALE-ADS seems potential as a water-proof coating with even a better performance than the commercial alginate, as a seed coating with an increased germination rate, and as a bio-adsorbent with a similar performance to the commercial alginate and ALE from aerobic sludge.

Giant tumefactive perivascular spaces.

Perivascular spaces are parts of the glymphatic pathway in the brain, which are microscopic but visible on magnetic resonance imaging when enlarged. Here, we described a case of a 16-year-old boy who presented with chronic headaches. Magnetic resonance imaging revealed giant perivascular spaces in the right centrum semiovale. Furtherly, we summarized the literature on classical and rare presentations of massive perivascular spaces and raised awareness that more clinical significance of the giant tumefactive perivascular spaces remains to be elucidated.

Low-temperature drying of waste activated sludge enhanced by agricultural biomass towards self-supporting incineration.

A high moisture content of waste activated sludge (WAS) associated with a low calorific value needs to be deeply dried towards self-supporting incineration. On the other hand, thermal energy with low temperature exchanged from treated effluent has great potential for drying sludge. Unfortunately, low-temperature drying of sludge seems to be low in efficiency and long in drying time. For this reason, some agricultural biomass was added into WAS to improve the drying efficiency. The drying performance and sludge properties were analyzed and evaluated with this study. Experimental results demonstrated that wheat straw was the best in enhancing the drying performance. With only 20 % (DS/DS) of crushed wheat straw added, the average drying rate achieved up to 0.20 g water/g DS·min, much higher than 0.13 g water/g DS·min of the raw WAS. The drying time to the targeted moisture content (63 %) (for self-supporting incineration) was shortened to only 12 min, much lower than 21 min of the raw WAS. The analysis revealed that wheat straw could reduce the specific resistance of filtration (SRF) and increase the sludge filterability (X). Also, the sludge rheology, particle size distribution and SEM images could conclude that agricultural biomass played a positive role in skeleton builders, forming a mesh-like structure in sludge flocs. These special channels could obviously improve the transfer capacities of heat and water inside the sludge matrix and thus greatly increase the drying performance of WAS.

Performance of a continuous flow reactor on bio-reducing vanadium with straw.

Vanadium (V) in groundwater could cause a serious threat to the environment and health. Continuous flow reactors were applied to reduce V(V) with straw being a solid carbon. The reduced efficiency of V(V) in the reactor with straw and inoculated sludge reached to 71.8%-99.9% for two months' operation (after 44 d). However, a long-term operation with only straw was not satisfied, achieving the reduced efficiency of 39.2-66.6%. The SEM images clearly revealed some traces of straw surface by utilized by microbes, which implied that microbes had a stronger capacity to hydrolyze straw. The introducing external microbes were essential to achieve a better bio-reduction performance on V(V). Treponema (5.3%) with metal reduction ability and Prevotellaceae (3.3%) able to specifically degrade complex plant-derived polysaccharides were found to be dominant in the microbial community. Utilizing agricultural biomass can save a lot of normal carbon like acetate, which is of benefit for carbon emissions.

Calcium Alginate Production through Forward Osmosis with Reverse Solute Diffusion and Mechanism Analysis.

Calcium alginate (Ca-Alg) is a novel target product for recovering alginate from aerobic granular sludge. A novel Ca-Alg production method was proposed herein where Ca-Alg was formed in a sodium alginate (SA) feed solution (FS) and concentrated via forward osmosis (FO) with Ca2+ reverse osmosis using a draw solution of CaCl2. An abnormal reverse solute diffusion was observed, with the average reverse solute flux (RSF) decreasing with increasing CaCl2 concentrations, while the average RSF increased with increasing alginate concentrations. The RSF of Ca2+ in FS decreased continuously as the FO progressed, using 1.0 g/L SA as the FS, while it increased initially and later decreased using 2.0 and 3.0 g/L SA as the FS. These results were attributed to the Ca-Alg recovery production (CARP) formed on the FO membrane surface on the feed side, and the percentage of Ca2+ in CARP to total Ca2+ reverse osmosis reached 36.28%. Scanning electron microscopy and energy dispersive spectroscopy also verified CARP existence and its Ca2+ content. The thin film composite FO membrane with a supporting polysulfone electrospinning nanofiber membrane layer showed high water flux and RSF of Ca2+, which was proposed as a novel FO membrane for Ca-Alg production via the FO process with Ca2+ reverse diffusion. Four mechanisms including molecular sieve role, electrification of colloids, osmotic pressure of ions in CARP, and FO membrane structure were proposed to control the Ca-Alg production. Thus, the results provide further insights into Ca-Alg production via FO along with Ca2+ reverse osmosis.

[N2O Emission from the Processes of Wastewater Treatment: Mechanisms and Control Strategies].

As a direct carbon emission source, the amount of nitrous oxide (N2, which is actually caused by AOB denitrification. To control the N2O emission during biological N-removal, complete HND and NO2- accumulation for AOB denitrification should be avoided to a large extent. For this purpose, DO in aerobic tanks should be controlled at a normal level (approximately 2 mg·L-1), and solid retention time (SRT) should be extended, up to 20 d, which would avoid accumulating N2O for AOB denitrification. Additionally, external carbon should be supplemented in time to promote HDN approaching the end, N2. This review summarizes the mechanisms of all the mentioned N2O emission pathways and discusses the control strategies of N2O emission according to the associated mechanisms.

Recovery of Extracellular Polymeric Substances from Excess Sludge Using High-Flux Electrospun Nanofiber Membranes.

The recycling of extracellular polymeric substances (EPSs) from excess sludge in wastewater treatment plants has received increasing attention in recent years. Although membrane separation has great potential for use in EPS concentration and recovery, conventional membranes tend to exhibit low water flux and high energy consumption. Herein, electrospun nanofiber membranes (ENMs) were fabricated using polyvinylidene fluoride (PVDF) and used for the recovery of EPSs extracted from the excess sludge using the cation exchange resin (CER) method. The fabricated ENM containing 14 wt.% PVDF showed excellent properties, with a high average water flux (376.8 L/(m2·h)) and an excellent EPS recovery rate (94.1%) in the dead-end filtration of a 1.0 g/L EPS solution at 20 kPa. The ENMs displayed excellent mechanical strength, antifouling properties, and high reusability after five recycles. The filtration pressure had a negligible effect on the average EPS recovery rate and water flux. The novel dead-end filtration with an EPS filter cake on the ENM surface was effective in removing heavy-metal ions, with the removal rates of Pb2+, Cu2+, and Cr6+ being 89.5%, 73.5%, and 74.6%, respectively. These results indicate the potential of nanofiber membranes for use in effective concentration and recycling of EPSs via membrane separation.

Extracting compositional blocks of alginate-like extracellular polymers (ALE) from conventional activated sludge (CAS).

As a highly added value material, alginate-like extracellular polymers (ALE) can be extracted from extracellular polymeric substances (EPS) from aerobic granular sludge (AGS). In fact, conventional activated sludge (CAS) also contains a certain amount of ALE. As CAS is widely used everywhere, waste activated sludge (WAS) from CAS is huge in its absolute amount. Although the ALE property of CAS was identified not so good as that from AGS, the mechanisms remains unclear. For this reason, it is necessary to unravel the chemically compositional blocks of ALE. Referring to natural alginate, ALE can be separated into three compositional blocks: GGL, GML and MML (like units containing guluronate or mannuronate), associated with other compositions including protein (PN), polysaccharide (PS), phosphorus (P), humic acid (HA). With real WAS from CAS, ALE was extracted and three blocks were separated: GGL = 54 %, GML = 42 % and MML = 4 % in weight, which is similar to the previous study. Moreover, the GGL blocks in CAS were obviously lower than AGS, down to by 1/3-1/2. And the GML and MML blocks in CAS were much higher than AGS, by more than 1/2. Different compositional blocks of ALE in AGS and CAS should be the reason forming different properties in applications. For this reason, a further study will be initiated to dispense/reorganize three blocks of ALE from CAS for expanding its potential applications, based on the compositional blocks of ALE from AGS.

Synergy of phosphate recovery from sludge-incinerated ash and coagulant production by desalinated brine.

Wet-chemical approach is widely applied for phosphate recovery from incinerated ash of waste activated sludge (WAS), along with metals removed/recovered. The high contents of both aluminum (Al) and iron (Fe) in WAS-incinerated ash should be suitable for producing coagulants with some waste anions like Cl- and SO42- With acid (HCl) leaching and metals' removing, approximately 88 wt% of phosphorus (P) in the ash could be recovered as hydroxylapatite (HAP: Ca5(PO4)3OH); Fe3+ in the acidic leachate could be selectively removed/recovered by extraction with an organic solvent of tributyl phosphate (TBP), and thus a FeCl3-based coagulant could be synthesized by stripping the raffinate with the original brine (containing abundant Cl- and SO42-). Furthermore, a liquid poly-aluminum chloride (PAC)-based coagulant could also be synthesized with Al3+ removed from the ash and the brine, which behaved almost the same in the coagulation performance as a commercial coagulant on both phosphate and turbidity removals. Both P-recovery from the ash and coagulant production associated with the brine would enlarge the markets of both 'blue' phosphate and 'green' coagulants.

Performance and Enhancement of Various Fillers Guiding Vanadium (V) Bioremediation.

Bioremediation of vanadium (V) pollution in groundwater is an emerging topic. However, knowledge of V in a biogeochemical process is limited and long-term effective removal methods are lacking. V(V) remediation processes by various kinds of auxiliary fillers (maifanite-1, maifanite-2, volcanic rock, green zeolite and ceramsite), agricultural biomass and microbial enhancing were explored in this study. In tests without inocula, the V(V) removal efficiencies of ceramsite (inert filler) and maifanite-2 (active filler) were 84.9% and 60.5%, respectively. When inoculated with anaerobic sludge, 99.9% of V(V) could be removed with the synergistic performance of straw and maifanite-2. TOC (Total Organic Carbon), trace elements and three-dimensional fluorescence analyses confirmed that maifanite-2 was the most suitable among various fillers in biological V(V) removal systems with straw. This study provides a collaborative method (adsorption-biology) by using straw with maifanite-2 in V(V)-contaminated groundwater. The knowledge gained in this study will help develop permeable reactive barrier technology to repair polluted groundwater to put forward a reasonable, effective and sustainable environmental treatment strategy.

Unravelling key factors controlling vivianite formation during anaerobic digestion of waste activated sludge.

As a product of phosphorous recovery from anaerobic digestion (AD) of waste activated sludge (WAS), vivianite has received increasing attention. However, key factors controlling vivianite formation have not yet been fully addressed. Thus, this study was initiated to ascertain key factors controlling vivianite formation. A simulation of chemical equilibriums indicates that interfering ions such as metallic ions and inorganic compounds may affect vivianite formation, especially at a PO43-concentration lower than 3 mM. The experiments demonstrated that the rate of ferric bio-reduction conducted by dissimilatory metal-reducing bacteria (DMRB) and the competition of methane-producing bacteria (MPB) with DMRB for VFAs (acetate) were not the key factors controlling vivianite formation, and that ferric bio-reduction of DMRB can proceed when a sufficient amount of Fe3+ exists in WAS. The determined affinity constants (Ks) of both DMRB and MPB on acetate revealed that the KHAc constant (4.2 mmol/g VSS) of DMRB was almost 4 times lower than that of MPB (15.67 mmol/g VSS) and thus MPB could not seriously compete for VFAs (acetate) with DMRB. As a result, vivianite formation was controlled mainly by the amount of Fe3+ in WAS. In practice, a Fe/P molar ratio of 2:1 should be enough for vivianite formation in AD of WAS. Otherwise, exogenously dosing Fe3+ or Fe2+ into AD must be applied in AD.

Metformin Preserves VE-Cadherin in Choroid Plexus and Attenuates Hydrocephalus via VEGF/VEGFR2/p-Src in an Intraventricular Hemorrhage Rat Model.

Hydrocephalus induced by intraventricular hemorrhage (IVH) is associated with unfavorable prognosis. The increased permeability of choroid plexus and breakdown of the blood-brain barrier (BBB) was reported as a prominent mechanism of IVH-induced hydrocephalus, and vascular endothelial-cadherin (VE-cadherin) was demonstrated to be relevant. Metformin was reported to protect endothelial junction and preserve permeability widely; however, its role in hydrocephalus remains unclear. In this study, the decreased expression of VE-cadherin in the choroid plexus, accompanied with ventricle dilation, was investigated in an IVH rat model induced by intraventricular injection of autologous blood. Metformin treatment ameliorated hydrocephalus and upregulated VE-cadherin expression in choroid plexus meanwhile. We then observed that the internalization of VE-cadherin caused by the activation of vascular endothelial growth factor (VEGF) signaling after IVH was related to the occurrence of hydrocephalus, whereas it can be reversed by metformin treatment. Restraining VEGF signaling by antagonizing VEGFR2 or inhibiting Src phosphorylation increased the expression of VE-cadherin and decreased the severity of hydrocephalus after IVH. Our study demonstrated that the internalization of VE-cadherin via the activation of VEGF signaling may contribute to IVH-induced hydrocephalus, and metformin may be a potential protector via suppressing this pathway.

Creating coagulants through the combined use of ash and brine.

Sludge incineration and seawater desalination are two approaches that can be used in the disposal of waste activated sludge (WAS) and for obtaining fresh water. As resource recovery from wastewater treatment and water purification is a topic of particular interest in these times, "water mining" has become a focus of research, with phosphate/P-recovery from WAS incineration ash, and extraction of useful elements from the brine of desalination being important steps in the pursuit of a circular/blue economy. However, P-recovery from ash involves removing metals, which need to be disposed of carefully, as does the brine collected. If cations in the ash and anions in the brine could be combined in order to produce coagulants/flocculants, a new circular model would be established. A preliminary experiment for this purpose has demonstrated that a liquid poly­aluminum chloride (PAC) could be synthesized from the aluminum ion/Al3+ removed from the ash and the original brine. With this work, we synthesized the liquid PAC by a hydrothermal method, and the results from infrared spectrometer demonstrated that the synthesized PAC was similar to a commercial PAC. Moreover, the synthesized PAC was able to efficiently reduce the effluent turbidity of wastewater treatment plants (WWTPs), especially when compared with the commercial PAC. It is therefore important that research in this area be continued in order to improve the quality of synthesized coagulants and to produce different coagulants based on cations and anions in ash and brine.

Making Waves: A sea change in treating wastewater - Why thermodynamics supports resource recovery and recycling.

Entropy is a concept defined by the second law of thermodynamics. Applying this concept to the world we live in, entropy production must be minimized and negentropy (negative entropy production) should be accelerated, in order to produce a healthy and stable ecological system. The present wastewater treatment, however, contributes to entropy production. This means that conventional wastewater treatment, without recovery of resource and energy, will gradually but inevitably contribute to a deteriorating ecological balance. When the self-cleaning ability of the natural ecological system is limited, the need to develop sustainable wastewater treatment in order to delay entropy production and accelerate negentropy becomes urgent. Resource and energy recovery from wastewater should be the first priority, as they can contribute significantly towards minimizing entropy production and accelerating negentropy. Sustainable wastewater treatment must focus on recovering recyclable high value-added organic chemicals from wastewater and/or excess sludge to minimize entropy production caused by methane (CH4, once combusted, is converted into CO2 - an even higher substance in entropy) via anaerobic digestion. Instead of CH4, thermal energy present in the effluent can be utilized for heating/cooling buildings and also for drying excess sludge towards incineration to recover more energy. Overall, this can lead to a carbon-neutral operation and even creating a "carbon sink" could be possible for wastewater treatment.

Enhancing extraction of alginate like extracellular polymers (ALE) from flocculent sludge by surfactants.

Alginate like extracellular polymers (ALE) recovered from flocculent sludge has been identified as a kind of highly valuable biomaterials. However, the extraction protocols limit the production of biopolymers as ALE extracted from flocculent sludge is at a lower level, around 90-190 mg/g VSS. Under this circumstance, the eco-friendly and effective optimizations for the ALE extraction protocols are expected, and thus surfactants have gained an attention to enhancing the ALE extraction. With this study, different surfactants with different structures and chemical characteristics, such as sodium dodecyl sulfate (SDS), cetyltrimethylammonium bromide (CTAB) and octyl phenyl polyoxyethylene ether (Triton X-100), were experimented to improve the ALE extraction, and in turn the optimal conditions and the associated mechanisms were evaluated and figured out. The experimental results indicated that surfactants could enhance the ALE extraction but also improve the alginate purification of ALE. With the optimal dosage of surfactants, the ALE extraction increased from 124.1 mg/g VSS to about 222.8-281.9 mg/g VSS, and the alginate purify was at around 54%-70%, in which the efficiency of the ALE extraction was improved by 79.5%-127.2%. Among others, Triton X-100 had the best performance on improving the ALE extraction, followed by CTAB and SDS. The mechanisms of surfactants on enhancing the ALE extraction and improving the alginate purify can be attributed to: i) surfactants micelles, which can solubilize flocs and extracellular biopolymers; ii) similar structures of surfactants and ALE, which follows the rule of "like dissolves like"; iii) functional groups adsorption, which facilitates the ALE release from matrixes. In a word, the optimized extraction protocol by using surfactants can be effectively applied to extract ALE from flocculent sludge.

Delayed Minocycline Treatment Ameliorates Hydrocephalus Development and Choroid Plexus Inflammation in Spontaneously Hypertensive Rats.

Hydrocephalus is a complicated disorder that affects both adult and pediatric populations. The mechanism of hydrocephalus development, especially when there is no mass lesion present causing an obstructive, is poorly understood. Prior studies have demonstrated that spontaneously hypertensive rats (SHRs) develop hydrocephalus by week 7, which was attenuated with minocycline. The aim of this study was to determine sex differences in hydrocephalus development and to examine the effect of minocycline administration after hydrocephalus onset. Male and female Wistar-Kyoto rats (WKYs) and SHRs underwent magnetic resonance imaging at weeks 7 and 9 to determine ventricular volume. Choroid plexus epiplexus cell activation, cognitive deficits, white matter atrophy, and hippocampal neuronal loss were examined at week 9. In the second phase of the experiment, male SHRs (7 weeks old) were treated with either saline or minocycline (20 mg/kg) for 14 days, and similar radiologic, histologic, and behavior tests were performed. Hydrocephalus was present at week 7 and increased at week 9 in both male and female SHRs, which was associated with greater epiplexus cell activation than WKYs. Male SHRs had greater ventricular volume and epiplexus cell activation compared to female SHRs. Minocycline administration improved cognitive function, white matter atrophy, and hippocampal neuronal cell loss. In conclusion, while both male and female SHRs developed hydrocephalus and epiplexus cell activation by week 9, it was more severe in males. Delayed minocycline treatment alleviated hydrocephalus, epiplexus macrophage activation, brain pathology, and cognitive impairment in male SHRs.