Acotarines A-G, new diterpenoid alkaloids from Aconitum taronense induce lysosomal biogenesis.

Seven new diterpenoid alkaloids, acotarines A-G (1-7), along with four known diterpenoid alkaloids, were isolated from Aconitum taronense. Their structures were established by 1D, 2D NMR spectra and HRESIMS data. Acotarine A (1) represents a franchetine-type alkaloid characterized by a N-butanone moiety. All the compounds were tested for their activities to induce lysosomal biogenesis through LysoTracker Red staining, compounds acotarines B (2), E (5), F (6), indaconitine (8), 7,8-epoxy-franchetine (9), and 6-epiforesticine (10) showed inducing lysosomal biogenesis activity.

Analysis of mexicanolide- and phragmalin-type limonoids from Heynea trijuga using high-performance liquid chromatography/electrospray tandem mass spectrometry.

RATIONALE: Limonoids, a class of tetranortriterpenoids, exhibit various biological effects, including acting as potent antifeedants and insect growth-regulators against various pests. The analysis of phragmalin- and mexicanolide-type limonoids by collision-induced dissociation tandem mass spectrometry (CID-MS/MS) has not been reported. METHODS: A high-performance liquid chromatography/electrospray ionization (HPLC/ESI)-MS/MS method was developed to investigate the fragmentation patterns of [M+NH4 ](+) ions for nine reference phragmalin- and mexicanolide-type limonoids. The method was also used in the identification of limonoid compounds in botanic extracts of Heynea trijuga. RESULTS: The losses of side chains and the furan part were the major fragmentation patterns. However, there was variation in the relative abundances of product ions resulting from the same fragmentation pathways. A total of 89 phragmalin- and mexicanolide-type limonoids in botanic extracts of Heynea trijuga were detected and 50 of these compounds were identified or tentatively characterized based on elemental constituents, fragmentation pathways, and the profile of the major product ions of reference compounds. In addition, the isomers could be tentatively distinguished. CONCLUSIONS: An HPLC/ESI-MS/MS method was developed and could be used to simultaneously identify and screen phragmalin- and mexicanolide-type limonoids in botanic extracts of Heynea trijuga.

Phragmalin- and mexicanolide-type limonoids from the leaves of Trichilia connaroides.

Phytochemical investigation of the leaves of Trichilia connaroides afforded 12 new limonoids with phragmalin- (1-11) and mexicanolide-type skeletons (12). The structures of these limonoids, including the absolute configuration of 3, were determined by spectroscopic analysis. Compounds 6 and 8 showed moderate cytotoxicity against HL-60 cells.