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1.
Fitoterapia ; 147: 104738, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-33031866

RESUMO

Seven new diterpenoid alkaloids, acotarines A-G (1-7), along with four known diterpenoid alkaloids, were isolated from Aconitum taronense. Their structures were established by 1D, 2D NMR spectra and HRESIMS data. Acotarine A (1) represents a franchetine-type alkaloid characterized by a N-butanone moiety. All the compounds were tested for their activities to induce lysosomal biogenesis through LysoTracker Red staining, compounds acotarines B (2), E (5), F (6), indaconitine (8), 7,8-epoxy-franchetine (9), and 6-epiforesticine (10) showed inducing lysosomal biogenesis activity.


Assuntos
Aconitum/química , Alcaloides/farmacologia , Diterpenos/farmacologia , Lisossomos/efeitos dos fármacos , Alcaloides/isolamento & purificação , China , Diterpenos/isolamento & purificação , Células HeLa , Humanos , Estrutura Molecular , Biogênese de Organelas , Compostos Fitoquímicos/isolamento & purificação , Compostos Fitoquímicos/farmacologia , Raízes de Plantas/química
2.
Rapid Commun Mass Spectrom ; 27(11): 1203-12, 2013 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-23650033

RESUMO

RATIONALE: Limonoids, a class of tetranortriterpenoids, exhibit various biological effects, including acting as potent antifeedants and insect growth-regulators against various pests. The analysis of phragmalin- and mexicanolide-type limonoids by collision-induced dissociation tandem mass spectrometry (CID-MS/MS) has not been reported. METHODS: A high-performance liquid chromatography/electrospray ionization (HPLC/ESI)-MS/MS method was developed to investigate the fragmentation patterns of [M+NH4 ](+) ions for nine reference phragmalin- and mexicanolide-type limonoids. The method was also used in the identification of limonoid compounds in botanic extracts of Heynea trijuga. RESULTS: The losses of side chains and the furan part were the major fragmentation patterns. However, there was variation in the relative abundances of product ions resulting from the same fragmentation pathways. A total of 89 phragmalin- and mexicanolide-type limonoids in botanic extracts of Heynea trijuga were detected and 50 of these compounds were identified or tentatively characterized based on elemental constituents, fragmentation pathways, and the profile of the major product ions of reference compounds. In addition, the isomers could be tentatively distinguished. CONCLUSIONS: An HPLC/ESI-MS/MS method was developed and could be used to simultaneously identify and screen phragmalin- and mexicanolide-type limonoids in botanic extracts of Heynea trijuga.


Assuntos
Cromatografia Líquida de Alta Pressão/métodos , Medicamentos de Ervas Chinesas/química , Limoninas/química , Espectrometria de Massas em Tandem/métodos , Estrutura Molecular , Espectrometria de Massas por Ionização por Electrospray/métodos
3.
J Nat Prod ; 74(2): 152-7, 2011 Feb 25.
Artigo em Inglês | MEDLINE | ID: mdl-21268637

RESUMO

Phytochemical investigation of the leaves of Trichilia connaroides afforded 12 new limonoids with phragmalin- (1-11) and mexicanolide-type skeletons (12). The structures of these limonoids, including the absolute configuration of 3, were determined by spectroscopic analysis. Compounds 6 and 8 showed moderate cytotoxicity against HL-60 cells.


Assuntos
Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Medicamentos de Ervas Chinesas/isolamento & purificação , Limoninas/química , Limoninas/isolamento & purificação , Meliaceae/química , Antineoplásicos Fitogênicos/farmacologia , Ensaios de Seleção de Medicamentos Antitumorais , Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/farmacologia , Células HL-60 , Humanos , Limoninas/farmacologia , Estrutura Molecular , Ressonância Magnética Nuclear Biomolecular , Folhas de Planta/química
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