Observations of TCRVß Gene Expression in Rats with Dampness Syndrome.

Environmental dampness is one factor which can cause human diseases. The effects of exposure to humidity on human immune function are diverse and numerous. In the theory of traditional Chinese medicine (TCM), dampness is defined as one of the major pathogenic factors in the human body. It is divided into "external dampness" and "internal dampness." However the molecular mechanism leading to humidity-induced immunosuppression is obscure. In the present study, we investigated the expression of the T-cell antigen receptor variable ß (TCRVß) subfamilies in rats which were fed in different humid environment. And the expression levels of the TCRVß subfamilies were detected using FQ-PCR. We found that the dampness might reduce the immunological recognition function of rats. And the obstruction of the immunological recognition function might be caused by internal dampness rather than external dampness.

Correlation between pathogenesis of dampness syndrome and interleukin-2, interleukin-8 in rats.

OBJECTIVE: To explore the pathogenesis of dampness syndrome by detecting the changes of interleukin-2 (IL-2) and interleukin-8 (IL-8) levels. METHODS: Female Sprague Dawley rats were divided randomly into five groups according to the random number table: a normal group (Group I), an external dampness group (Group II), an internal dampness group (Group III), and an external and internal dampness group (Group IV). Twenty days after the model made, IL-2 and IL-8 levels were detected by radioimmunoassay method. RESULTS: The IL-2 and IL-8 levels among groups were significant (F = 3.102, P < 0.05; F = 2.657, P < 0.05, respectively). The level of IL-2 in Group II and Group III were higher than that in Group I (P < 0.05, P < 0.01, respectively), especially higher in the Group III compared with Group II (P < 0.05). The level of IL-8 in Group III were higher than those in Group I, Group II and Group IV (P < 0.05, P < 0.01, P < 0.05, respectively). In the Group III, the 24-hour water and body weight were higher than that in the Group IV (all P < 0.05), and spontaneous movement frequency was higher than those in Group II and Group IV (P < 0.05). CONCLUSION: Immune activation and inflammatory reaction might be easily caused by external dampness other than internal dampness.