RESUMO
Cell suspensions of Marchantia polymorpha hydrogenate progesterone to 5alpha-pregnane-3,20-dione. Structure elucidation of the product was achieved by comprehensive NMR analyses.
Assuntos
Marchantia/metabolismo , Progesterona/metabolismo , Monoterpenos Bicíclicos , Biotransformação , Células Cultivadas/metabolismo , Marchantia/citologia , Progesterona/química , Terpenos/química , Terpenos/metabolismoRESUMO
Microbial transformation of 13R,14R,15-trihydroxylabd-7-ene (5) and 13R,14R,15-trihydroxylabd-8(17)-ene (6) by the fungus Debaryomyces hansenii gave 1 (13R,14R,15-trihydroxy-6-oxolabd-8-ene) and 3 (7alpha,13R,14R,15-tetrahydroxy-labd-8(17)-ene), respectively. While, microbial transformation of 5 by Aspergillus niger afforded 2 (3beta,13R,14R,15-tetrahydroxy-labd-7-ene), and 13R,14R,15-trihydroxylabd-8,17-ene (6) gave 3 and 4 (3R,14R,15-3-oxotetrahydroxy-labd-7-ene). The structures of the new compounds, 1 and 2, were assigned by 1D and 2D high-field NMR spectroscopic methods. Antimicrobial activity of these compounds were tested and their MIC were determined.
