An Anatomical Dissection Method for Observation of Fibrous Facial Structures.

BACKGROUND: In recent years, structures including the superficial musculoaponeurotic system and retaining ligaments that support the facial soft tissue have been clarified. However, these structures are very difficult to observe in their entirety by the standard gross anatomical procedure (ie, dissection from superficial to deep layers). Furthermore, accurate descriptions of these structures are rare in both anatomical and plastic surgery textbooks. The aim of this study was to clarify the facial fibrous structures in a gross anatomical view. METHODS: The authors' novel method used soft facial tissue and bone. The tissue was fixed in gelatin and sectioned at a thickness of 5 to 10 mm. Each section was placed on a wooden board; the bone was then pinned, and the skin was pulled outward with sutures to hyperextend the soft tissue. Subsequently, the loose connective tissue was torn and fat tissue was removed under a surgical microscope. After the removal of fat tissue, the fibrous facial structures (eg, the superficial musculoaponeurotic system and retaining ligaments) could be observed clearly. RESULTS: The thickness of the sections allowed three-dimensional observation, such that a structure located deep within a section could be clearly observed. The expansion of soft tissue facilitated observation of the facial layer and fibrous structures, and the locations of nerves and vessels. Therefore, the facial layer structure was readily discerned. CONCLUSION: This method is likely to be very useful in the field of plastic surgery because it enabled intuitive identification of facial layers and their characteristics. CLINICAL RELEVANCE STATEMENT: The dissection method developed by the authors reveals the connected morphology of each tissue of the face, thus providing basic data for analyzing soft tissue changes due to aging and gravity. This will be useful for the development of anti-aging medicine.

Closure of Intractable Enterocutaneous Fistula with a Rectus Abdominis Musculocutaneous Flap.

Large enterocutaneous fistulas of the small intestine are rare and difficult to close, particularly if the fistula is associated with massive leakage of digestive juice and the residual intestinal tract is too short for anastomosis. We present a patient who underwent small bowel resection and secondary anastomosis following massive necrosis of the small intestine due to superior mesenteric artery thrombosis. After resection of an enterocutaneous fistula and reanastomosis, the residual small bowel was only 70 cm long with a persistent fistula. We successfully closed the fistula by employing a hinged rectus abdominis musculocutaneous flap. Here, we report our procedure for treating a large enterocutaneous fistula without performing laparotomy and bowel resection.

Novel Cadaver Injection Method Using Latex and Magnetic Fluid.

Vascular injection into extracted tissue may be associated with leakage due to excessive local injection pressure. Historically, this complication has been impossible to resolve because the injection pressure has been the only available force with which to send the agent to the peripheral vasculature. We have developed a new vascular injection method that utilizes a material affected by magnetic force and is therefore not solely dependent upon the injection pressure. We mixed the same weights of latex and magnetic fluid and injected the solution into the arterial stump of an extracted tissue specimen. Next, we used a permanent magnet to attract the agent into the peripheral vasculature. We repeated the injection and magnetic application until no further fluid could be injected. We used this method in 20 formalin-fixed tissue specimens. The vessels were clearly observable through to the peripheral areas, and leakage from the injected artery was minimal. This new agent has several beneficial characteristics: it is X-ray impermeable, is durable in the face of chemical insult, and allows for easy visual observation. The injected tissue can be studied for X-ray film examination, tissue clarification, and gross anatomical dissection. Additionally, this method can be applied to both fresh and formalin-fixed tissue. We consider that this method has the potential to expand the applications of injection studies.

A new modified Pudendal Thigh Flap of vaginoplasty including reconstruction of vaginal vestibule.

We modified the conventional Pudendal Thigh Flap (PTF) on the vaginoplasty including reconstruction of vaginal vestibule. After the operation, no stenosis of the vaginal vestibule and opening of the vagina was observed. It is believed that our technique is cosmetically and functionally possible and a useful method.