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1.
Acta Chir Orthop Traumatol Cech ; 82(1): 48-50, 2015.
Artigo em Tcheco | MEDLINE | ID: mdl-25748661

RESUMO

PURPOSE OF THE STUDY: The study was designed to investigate whether anatomical variations of the anterior and posterior divisions of the internal iliac artery and their branches are associated with different risks of bleeding resulting from injury to the posterior pelvic segment. MATERIAL AND METHODS: The study was carried out on 19 cadavers. The dissected area included the internal iliac artery from the common iliac artery bifurcation to the origins of the superior gluteal artery, the inferior gluteal artery and the internal pudendal artery. Using an electronic slide rule, distances between the bifurcation and the origin of each branch from either the anterior or the posterior division were measured. The diameter of each vessel was also determined. Findings of the study were compared with variations described in the literature. The degree of risk for bleeding related to different anatomical variations of the internal iliac artery and its branches was evaluated based on the proximity to the bone. RESULTS: There are six anatomical variations of internal iliac artery branches. Four of them were found: type A1 was recorded in 10 specimens, type A2 in six, type B1 in two and type C in one specimen. Types B2 and D were not seen. DISCUSSION: The type B2 and C anatomical variations were considered to carry higher risks of bleeding due to injury to the posterior pelvic segment. These variations are characterized by vessels larger in diameter and a longer course of the posterior division along the posterior part of the greater sciatic notch (area often involved in unstable pelvic ring fractures). On the other hand, the type C variation showed a longer internal iliac artery separated from the bone with a thick layer of soft tissue, which suggested lower risk than was attributed to the dominant type A1 variation. It was not possible to evaluate type B2 variation because it is very rare and was not found in study material. In type A2 and B1 variations, the branches were separated from bony structures similarly to the dominant type A1 variation. CONCLUSIONS: The cadaver study designed to assess the risk of bleeding associated with different morphological variations of the branching pattern of the internal iliac artery did not identify any anatomical arrangement that might carry a higher risk of injury to the vessels by free bone fragments of the posterior segment in unstable pelvic fractures. It can be concluded that less common branching patterns of the internal iliac artery are not associated with higher risk of bleeding than the dominant type A1 variation.


Assuntos
Fraturas Ósseas/complicações , Hemorragia/etiologia , Artéria Ilíaca/lesões , Ossos Pélvicos/lesões , Cadáver , Humanos , Artéria Ilíaca/anatomia & histologia , Medição de Risco/métodos
2.
Neoplasma ; 59(1): 62-9, 2012.
Artigo em Inglês | MEDLINE | ID: mdl-22103898

RESUMO

Zeocin is a member of bleomycin/phleomycin family of antibiotics isolated from Streptomyces verticullus. This unique radiomimetic antibiotic is known to bind to DNA and induce oxidative stress in different organisms producing predominantly single- and double- strand breaks, as well as a DNA base loss resulting in apurinic/apyrimidinic (AP) sites. The aim of this study was to induce an adaptive response (AR) by zeocin in freshly isolated human lymphocytes from blood and to observe whether plant extracts could modulate this response. The AR was evaluated by the comet assay. The optimal conditions for the AR induction and modulation were determined as: 2 h-intertreatment time (in PBS, at 4°C) given after a priming dose (50 µg/ml) of zeocin treatment. Genotoxic impact of zeocin to lymphocytes was modulated by plant extracts isolated from Gentiana asclepiadea (methanolic and aqueous haulm extracts, 0.25 mg/ml) and Armoracia rusticana (methanolic root extract, 0.025 mg/ml). These extracts enhanced the AR and also decreased DNA damage caused by zeocin (after 0, 1 and 4 h-recovery time after the test dose of zeocin application) to more than 50%. These results support important position of plants containing many biologically active compounds in the field of pharmacology and medicine.


Assuntos
Antibacterianos/toxicidade , Armoracia/química , Bleomicina/toxicidade , Regulação da Expressão Gênica/efeitos dos fármacos , Gentiana/química , Extratos Vegetais/farmacologia , Adaptação Biológica/efeitos dos fármacos , Antibacterianos/administração & dosagem , Antibacterianos/antagonistas & inibidores , Antibacterianos/farmacologia , Bleomicina/administração & dosagem , Bleomicina/antagonistas & inibidores , Bleomicina/farmacologia , Ensaio Cometa , Quebras de DNA de Cadeia Dupla/efeitos dos fármacos , Quebras de DNA de Cadeia Simples/efeitos dos fármacos , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos , Interações Medicamentosas , Eletroforese em Gel de Ágar , Humanos , Linfócitos/citologia , Linfócitos/efeitos dos fármacos , Metanol , Testes de Mutagenicidade , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/isolamento & purificação , Raízes de Plantas/química , Solventes , Água
3.
Neoplasma ; 58(5): 386-91, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-21744991

RESUMO

Varied medicinal plants are known as a source of natural phytochemicals with antioxidant activities that can protect organisms from oxidative stress and from various chronic diseases. Papaver rhoeas has a long history of medicinal usage, especially for ailments in adults and children. The possible cytotoxicity, genotoxicity and potential antioxidant effect of plant extract isolated from flowers of Papaver rhoeas was investigated in human lymfoblastoid cell line (TK6). Antioxidant activity of this extract was determined using the DPPH assay. The plant extract exhibited dose dependent free radical scavenging ability. The growth activity assay was used for determination of cytotoxicity. To assess potential genotoxicity the comet assay was used. The lower extract concentrations (0.25 and 0.5 mg/ml) neither exerted cytotoxic, nor genotoxic effects in TK6 cells but they stimulated cell proliferation. The concentration 25 mg/ml scavenged almost 85% of DPPH free radical. On the other hand, this concentration had strong cytotoxic and genotoxic effect on TK6 cells. The balance between beneficial and harmful effects should be always considered when choosing the effective dose.


Assuntos
Antioxidantes/farmacologia , Proliferação de Células/efeitos dos fármacos , Dano ao DNA/efeitos dos fármacos , Sequestradores de Radicais Livres/farmacologia , Linfócitos/efeitos dos fármacos , Papaver/química , Extratos Vegetais/farmacologia , Células Cultivadas , Ensaio Cometa , Radicais Livres/metabolismo , Humanos , Linfócitos/citologia , Estresse Oxidativo/efeitos dos fármacos , Extratos Vegetais/isolamento & purificação
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