Evaluation of the cerebral hemodynamic response to rhythmic handgrip.

The response of the cerebral circulation to exercise has been studied with transcranial Doppler ultrasound (TCD) because this modality provides continuous measurements of blood velocity and is well suited for the exercise environment. The use of TCD as an index of cerebral blood flow, however, requires the assumption that the diameter of the insonated vessel is constant. Here, we examine this assumption for rhythmic handgrip using a spectral index designed to measure trends in vessel flow. Nineteen normal subjects were studied during 5 min of volitional maximum rhythmic right handgrip at 1 Hz. TCD velocities from both middle arteries (left and right), blood pressure, and end-tidal PCO(2) were recorded every 10 s. A spectral weighted sum was also calculated as a flow index (FI). Averages were computed from the last 2 min of handgrip. Relative changes in velocity, FI, and pressure were calculated. The validity of FI was tested by comparing the change in diameter derived from equations relating flow and diameter. Mean blood pressure increased 23.8 +/- 17.8% (SD), and velocity increased 13.3 +/- 9.8% (left) and 9.6 +/- 8.3% (right). Although the mean change in FI was small [2.0 +/- 18. 2% (left) and 4.7 +/- 29.7% (right)], the variation was high: some subjects showed a significant increase in FI and others a significant decrease. Diameter estimates from two equations relating flow and luminal area were not significantly different. Decreases in FI were associated with estimated diameter decreases of 10%. Our data suggest that the cerebral blood flow (CBF) response to rhythmic handgrip is heterogeneous and that middle cerebral artery flow can decrease in some subjects, in agreement with prior studies using the Kety-Schmidt technique. We speculate that the velocity increase is due to sympathetically mediated vasoconstriction rather than a ubiquitous flow increase. Our data suggest that the use of ordinary TCD velocities to interpret the CBF response during exercise may be invalid.

Oscillations in cerebral blood flow detected with a transcranial Doppler index.

Although transcranial Doppler ultrasound (TCD) has been used to detect oscillations in CBF, interpretation is severely limited, since only blood velocity and not flow is measured. Oscillations in vessel diameter could, therefore, mask or alter the detection of those in flow by TCD velocities. In this report, the authors use a TCD-derived index of flow to detect and quantify oscillations of CBF in humans at rest. A flow index (FI) was calculated from TCD spectra by averaging the intensity weighted mean in a beat-by-beat manner over 10 seconds. Both FI and TCD velocity were measured in 16 studies of eight normal subjects at rest every 10 seconds for 20 minutes. End tidal CO2 and blood pressure were obtained simultaneously in six of these studies. The TCD probe position was meticulously held constant. An index of vessel area was calculated by dividing FI by velocity. Spectral estimations were obtained using the Welch method. Spectral peaks were defined as peaks greater than 2 dB above background. The frequencies and magnitudes of spectral peaks of FI, velocity, blood pressure, and CO2 were compared with t tests. The Kolmogorov-Smirnov test was used to further confirm that the data were not white noise. In most cases, three spectral peaks (a, b, c) could be identified, corresponding to periods of 208+/-93, 59+/-31, and 28+/-4 (SD) seconds for FI, and 196+/-83, 57+/-20, and 28+/-6, (SD) seconds for velocity. The magnitudes of the spectral peaks for FI were significantly greater (P<0.02) than those for velocity. These magnitudes corresponded to variations of at least 15.6%, 9.8%, and 6.8% for FI, and 4.8%, 4.2%, and 2.8% for velocity. The frequencies of the spectral peaks of CO2 were similar to those of FI with periods of 213+/-100, 60+/-46, and 28+/-3.6 (SD) seconds. However, the CO2 spectral peak magnitudes were small, with an estimated maximal effect on CBF of (+/-) 2.5+/-0.98, 1.5+/-0.54, and 1.1+/-0.31 (SD) percent. The frequencies of the blood pressure spectral peaks also were similar, with periods of 173+/-81, 44+/-8, and 26+/-2.5 (SD) seconds. Their magnitudes were small, corresponding to variations in blood pressure of (+/-) 2.1+/-0.55, 0.97+/-0.25, and 0.72+/-0.19 (SD) percent. Furthermore, coherence analysis showed no correlation between CO2 and FI, and only weak correlations at isolated frequencies between CO2 and velocity, blood pressure and velocity, or blood pressure and FI. The Kolmogorov-Smirnov test distinguished our data from white noise in most cases. Oscillations in vessel flow occur with significant magnitude at three distinct frequencies in normal subjects at rest and can be detected with a TCD-derived index. The presence of oscillations in blood velocity at similar frequencies but at lower magnitudes suggests that the vessel diameters oscillate in synchrony with flow. Observed variations in CO2 and blood pressure do not explain the flow oscillations. Ordinary TCD velocities severely underestimate these oscillations and so are not appropriate when small changes in flow are to be measured.

Estimation of vessel flow and diameter during cerebral vasospasm using transcranial Doppler indices.

OBJECTIVE: An important limitation of transcranial Doppler (TCD) ultrasonography is its inability to directly measure blood flow or vessel diameter. To extend the ability of TCD ultrasonography, indices were derived from an intensity-weighted mean of the entire Doppler spectrum. The objective of this article is to test the behavior of these indices under conditions of diameter constancy (hyper- and hypoventilation) and when vessel diameter decreases (vasospasm). METHODS: A flow index (FI) was calculated by averaging several heartbeats of spectral data and calculating the first spectral moment. An area index (AI) was defined as the FI divided by the mean velocity, motivated by the knowledge that vessel flow is the product of vessel diameter and mean velocity. To test the FI and the AI under conditions of diameter constancy, middle cerebral artery Doppler signals were obtained from 20 patients during conditions of hypercarbia, hypocarbia, and normocarbia. To test the ability of these indices to evaluate a decrease in vessel diameter, signals from 41 sites on 23 arteries were obtained from patients who underwent both TCD and angiographic studies on two separate occasions after the occurrence of subarachnoid hemorrhage. The changes in the AI were compared with the arterial diameters measured from angiograms. RESULTS: The FI was proportional to the mean velocity in the cohort of healthy patients (r=0.97). The AI changed by less than 3% in the same cohort. The AI predicted the direction of the diameter change in all vessels showing angiographic changes in area. Changes in the AI and the measured angiographic changes in cross-sectional areas were correlated (overall, r=0.90; with two outlines removed, r=0.86). CONCLUSION: This variant of the intensity-weighted mean predicts changes in vessel cross-sectional area under conditions of changes in CO2 and cerebral vasospasm. This preliminary study suggests that careful use of this tool may provide accurate evaluation of cerebral blood flow through the large vessels and quantitative changes in diameter, which occur frequently after subarachnoid hemorrhage.

Cerebral blood flow and cranial magnetic resonance imaging in eclampsia and severe preeclampsia.

OBJECTIVE: To measure cerebral blood flow in women with eclampsia and severe preeclampsia using phase-contrast magnetic resonance imaging (MRI). METHODS: Women with eclampsia and severe preeclampsia were studied and compared with normotensive cohorts. Magnetic resonance imaging studies were performed initially in hypertensive women after seizure treatment or prophylaxis was given. Magnetic resonance imaging flow measurements were made using a phase contrast velocity imaging technique in each middle and posterior cerebral artery. Conventional brain MRI and magnetic resonance angiography of the circle of Willis were performed at the time of flow measurement. Women with preeclampsia and eclampsia served as their own controls and were matched with normotensive cohorts. All of the hypertensive women were studied again 4-5 weeks postpartum. Paired t test analysis and an analysis of variance were performed. Considering a 20% minimum detectable difference in flow, the power was 0.80, 0.92, 0.86, and 0.96 for the left and right middle cerebral arteries and the left and right posterior cerebral arteries, respectively. RESULTS: All 28 women enrolled were studied initially within 24 hours of delivery or of their most recent seizure. There were no significant differences in blood flow in either the posterior or middle cerebral arteries in women with eclampsia or severe preeclampsia between the initial studies and those 4-5 weeks postpartum, or compared with their normal counterparts. No findings of vasospasm were seen. T2-weighted brain images were markedly abnormal in all eight women with eclampsia, mildly abnormal in two of ten with severe preeclampsia, and normal in all ten controls. CONCLUSIONS: No flow changes were seen in the posterior or middle cerebral arteries of women with eclampsia and severe preeclampsia despite the presence of remarkable brain lesions in all women with eclampsia. These findings question the role of vasospasm and cerebral hypoperfusion, although a vasodilatory effect of magnesium could not be excluded.

Evaluation of cerebral arterial flow with transcranial Doppler ultrasound: theoretical development and phantom studies.

Blood flow information available from transcranial Doppler ultrasound is usually derived from velocity alone because no knowledge of vessel caliber is available. In cases such as vasospasm, where vessel size changes, the inference of flow from velocity becomes questionable. A computational technique was used to calculate a flow index and 2 vessel area indices based on the first and zero moments of the Doppler power spectrum. These indices were tested in a steady and pulsatile flow phantom using 6 different diameter elastic tubes. Changes in the flow index showed good agreement with changes in timed volume flow for different flow rates. The vessel caliber indices correctly predicted changes in area when different diameter tubes were examined. These indices may prove useful in clinical settings where the constancy of flow or vessel diameter between studies are in question.