Lagoecia cuminoides L., its antioxidant activity and polyphenolic constituents from Iran.

Lagoecia cuminoides L. belongs to the family of Umbelliferae (Apiaceae), and known also as common wild cumin. The aerial parts of L. cuminoides were collected at the flowering stage and dried, then the methanolic extract was analyzed for polyphenol compounds identified by HPLC-DAD and antioxidant activity (DPPH(2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay). It was found that the predominant phenolic constituents were chlorogenic acid, hesperidin, rosmarinic acid, hesperetin and vanillin. The antioxidant activity of methanolic extract from L. cuminoides was found 1597 µg/mL in DPPH scavenging assay. There is no strict positive relationship between the polyphenolic content and antioxidant activity of extracts.

Gontscharovia popovii, a new source of carvacrol, its polyphenolic constituents, essential oil analysis, total phenolic content and antioxidant activity.

The experiment was carried out using the shadow-dried aerial parts including leaves and shoots of Gontscharovia popovii collected in Fars province in order to investigate the polyphenolic compositions, antioxidant activity, total phenolic content and essential oil constituents. The result showed IC50 of 395.77 µg mL-1 and total phenolic content of about 20.01 mg g-1 gallic acid equivalent dry weight. It also showed a wild range of polyphenols such as; Gallic acid, catechin, chloregenic acid, rutin, vanillin, trans-Ferulic acid, sinapic acid, coumarin, hesperedin, quercetin, hesperetin, eugenol and carvacrol as the main detected polyphenols. Some major compounds were also detected through essential oil analysis, such as; 76.7% carvacrol, 4.25% γ-Terpinene, 3.8% p-Cymene and 2.4% (E)-Caryophyllene. Qualitative and quantitative analyses of chemical compounds of G. popovii was performed using HPLC, GC, GC/MS and microplate reader.

The constituents of essential oil in leaves of Karaj accession of Trigonella foenum graecum.

The chemical composition of the essential oils of Karaj accession of Trigonella foenum graecum leaves was detected by hydro-distillation and analysed by gas chromatography (GC-FID) and gas chromatography-mass spectroscopy (GC-MS) apparatuses for first time. Thirty-six compounds representing 95.3% of the total components were identified. The patterns of the main compounds were (2E)-Hexenal (26.61%), n-Hexadecanoic acid (10.14%) and (E)-b-Ionone (7.99%). Other notable constituents were Thymol (4.79%), 6,10,14-trimethyl-2-Pentadecanone (4.59%), Carvacrol (3.40%), (E)-Nerolidol (3.32%) and (2E,6Z)-Nonadienal (3.30%). (2E)-Hexenal was found as the most dominant component in this study.

Essential oil composition, free radical scavenging activity and polyphenolic content of Gaillonia eriantha Jaub. & Spach from Iran.

Gaillonia eriantha is a member of family Paederieae (Rubiaceae). The essential oil of G. eriantha growing wild in south of Iran was obtained by hydrodistillation and analysed by gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). Antioxidante activity and polyphenolic content were identified by DPPH microplate method and HPLC-DAD, respectively. Twenty-two compounds were identified in the oil. The major volatile compounds were camphor (14.8%), octyl formate (14.7%), 1,8-cineole (10.9%), n-decane (8.1%), n-undecane (6.8%), n-dodecane (6.1%), n-nonane(5.4%) and α-agarofuran (5.2%). The antioxidant activity of the extract of G. eriantha was found to be 786.57 µg/mL. It was found that the predominant phenolic constituents were chlorogenic acid (2.23 mg/g), catechin (0.37 mg/g), hesperidin (0.13 mg/g), trans-ferulic acid (0.12 mg/g), caffeic acid (0.051 mg/g), carvacrol (0.028 mg/g) and vanillin (0.021 mg/g), respectively.

Comparative essential oil composition of aerial parts of Tanacetum dumosum Boiss. from Southern Zagros, Iran.

The essential oils of leaves and flowers of Tanacetum dumosum Boiss., an endemic medicinal shrub, were extracted by using hydrodistillation method and analysed using GC and GC-MS. A total of 43 and 44 compounds were identified in the essential oils from the leaves and flowers of T. dumosum, respectively. The major chemical constituents of leaves oil were borneol (27.9%), bornyl acetate (18.4%), 1,8-cineol (17.5%), α-terpineol (5.3%), cis-chrysanthenyl acetate (3.3%), camphene (2.7%) and terpinene-4-ol (1.9%), while the main components of the flower oil were isobornyl-2-methyl butanoate (41.1%), trans-linalyl oxide acetate (11.9%), 1,8-cineole (7.7%), thymol (4.2%), linalool (3.9%), camphor (2.9%), isobornyl propanoate (2.9%), α-terpineol (2.1%) and caryophyllene oxide (2.0%). Major qualitative and quantitative variations for some main chemical compounds among different aerial parts of T. dumosum were identified. High contents of borneol, bornyl acetate, 1,8-cineol and linalool in the leaves and flowers of T. dumosum show its potential for use in the food and perfumery industry.

Human radiation dosimetry of 6-[18F]FDG predicted from preclinical studies.

PURPOSE: The authors are developing 6-[(18)F]fluoro-6-deoxy-D-glucose (6-[(18)F]FDG) as an in vivo tracer of glucose transport. While 6-[(18)F]FDG has the same radionuclide half-life as 2-[(18)F]fluoro-2-deoxy-D-glucose (2-[(18)F]FDG) which is ubiquitously used for PET imaging, 6-[(18)F]FDG has special biologic properties and different biodistributions that make it preferable to 2-[(18)F]FDG for assessing glucose transport. In preparation for 6-[(18)F]FDG use in human PET scanning, the authors would like to determine the amount of 6-[(18)F]FDG to inject while maintaining radiation doses in a safe range. METHODS: Rats were injected with 6-[(18)F]FDG, euthanized at specified times, and tissues were collected and assayed for activity content. For each tissue sample, the percent of injected dose per gram was calculated and extrapolated to that for humans in order to construct predicted time-courses. Residence times were calculated as areas under the curves and were used as inputs to OLINDA/EXM in order to calculate the radiation doses. RESULTS: Unlike with 2-[(18)F]FDG for which the urinary bladder wall receives the highest absorbed dose due to urinary excretion, with 6-[(18)F]FDG there is little urinary excretion and osteogenic cells and the liver are predicted to receive the highest absorbed doses: 0.027 mGy/MBq (0.100 rad/mCi) and 0.018 mGy/MBq (0.066 rad/mCi), respectively. Also, the effective dose from 6-[(18)F]FDG, i.e., 0.013 mSv/MBq (0.046 rem/mCi), is predicted to be approximately 30% lower than that from 2-[(18)F]FDG. CONCLUSIONS: 6-[(18)F]FDG will be safe for use in the PET scanning of humans.

Chemical constituents of the essential oil from aerial parts and fruit of Anisosciadium orientale.

The essential oils from aerial parts and fruits of Anisosciadium orientale DC. growing wild in Iran were obtained by hydrodistillation and analyzed by GC and GC/MS. Seventy-one compounds were identified in the fruit oil and fifteen in the oil from the aerial parts. The main oil components of the fruits and aerial parts were myristicin (33.5%-33.7%), alpha-terpinolene (22%-25.8%) and limonene (19.5%-19.7%). Some compounds, such as geranyl butyrate and germacrene-D, were only detected in the fruit oil.