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1.
J Exp Biol ; 227(3)2024 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-38197515

RESUMO

Vectors of infectious disease include several species of Aedes mosquitoes. The life cycle of Aedes aegypti, the yellow fever mosquito, consists of a terrestrial adult and an aquatic larval life stage. Developing in coastal waters can expose larvae to fluctuating salinity, causing salt and water imbalance, which is addressed by two prime osmoregulatory organs - the Malpighian tubules (MTs) and anal papillae (AP). Voltage-gated ion channels (VGICs) have recently been implicated in the regulation of ion transport in the osmoregulatory epithelia of insects. In the current study, we: (i) generated MT transcriptomes of freshwater-acclimated and brackish water-exposed larvae of Ae. aegypti, (ii) detected expression of several voltage-gated Ca2+, K+, Na+ and non-ion-selective ion channels in the MTs and AP using transcriptomics, PCR and gel electrophoresis, (iii) demonstrated that mRNA abundance of many altered significantly following brackish water exposure, and (iv) immunolocalized CaV1, NALCN, TRP/Painless and KCNH8 in the MTs and AP of larvae using custom-made antibodies. We found CaV1 to be expressed in the apical membrane of MTs of both larvae and adults, and its inhibition to alter membrane potentials of this osmoregulatory epithelium. Our data demonstrate that multiple VGICs are expressed in osmoregulatory epithelia of Ae. aegypti and may play an important role in the autonomous regulation of ion transport.


Assuntos
Aedes , Febre Amarela , Animais , Aedes/fisiologia , Água/metabolismo , Túbulos de Malpighi/metabolismo , Febre Amarela/metabolismo , Mosquitos Vetores , Cloreto de Sódio/metabolismo , Transporte de Íons , Canais Iônicos/genética , Larva/fisiologia
2.
G3 (Bethesda) ; 12(7)2022 07 06.
Artigo em Inglês | MEDLINE | ID: mdl-35639673

RESUMO

Here, we report the discovery of a novel Sediminibacterium sequenced from laboratory cultures of freshwater stream cyanobacteria from sites in Southern California, grown in BG11 medium. Our genome-wide analyses reveal a highly contiguous and complete genome (97% BUSCO) that is placed within sediminibacterial clades in phylogenomic analyses. Functional annotation indicates the presence of genes that could be involved in mutualistic/commensal relationship with associated cyanobacterial hosts.


Assuntos
Cianobactérias , Rios , Cianobactérias/genética , Água Doce/microbiologia , Estudo de Associação Genômica Ampla , Filogenia
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