Microwave induced "egg yolk" structure in Cr/V-MIL-53.

Using a one pot microwave procedure, mixed-metal "egg yolk" MOFs are created, with a core of (Cr/V)-MIL-53 and a shell of Cr-MIL-53. In contrast, the solvothermal method produces homogeneous mixed-metal MOFs. The influence of Cr and V on the flexibility and breathing was studied via T-XRPD and CO2 adsorption measurements.

Constitutively Active Arabidopsis MAP Kinase 3 Triggers Defense Responses Involving Salicylic Acid and SUMM2 Resistance Protein.

Mitogen-activated protein kinases (MAPKs) are important regulators of plant immunity. Most of the knowledge about the function of these pathways is derived from loss-of-function approaches. Using a gain-of-function approach, we investigated the responses controlled by a constitutively active (CA) MPK3 in Arabidopsis thalianaCA-MPK3 plants are dwarfed and display a massive derepression of defense genes associated with spontaneous cell death as well as the accumulation of reactive oxygen species, phytoalexins, and the stress-related hormones ethylene and salicylic acid (SA). Remarkably CA-MPK3/sid2 and CA-MPK3/ein2-50 lines, which are impaired in SA synthesis and ethylene signaling, respectively, retain most of the CA-MPK3-associated phenotypes, indicating that the constitutive activity of MPK3 can bypass SA and ethylene signaling to activate defense responses. A comparative analysis of the molecular phenotypes of CA-MPK3 and mpk4 autoimmunity suggested convergence between the MPK3- and MPK4-guarding modules. In support of this model, CA-MPK3 crosses with summ1 and summ2, two known suppressors of mpk4, resulted in a partial reversion of the CA-MPK3 phenotypes. Overall, our data unravel a novel mechanism by which the MAPK signaling network contributes to a robust defense-response system.

Size-tunable, bright, and stable PbS quantum dots: a surface chemistry study.

PbS Qdots are synthesized using PbCl2 and elemental sulfur as precursors. The available size range is significantly expanded using tri-n-octylphosphine (TOP), enabling the synthesis of monodisperse suspensions of Qdots with a mean size varying between 3 and 10 nm. The ligand composition and dynamics are investigated with nuclear magnetic resonance (NMR) spectroscopy. We show that the Qdots are passivated solely by highly dynamic OlAm ligands, even when TOP is employed during synthesis. In this respect, TOP is a compound strongly modifying the Qdot synthesis, without affecting the final Qdot surface chemistry. Next, the OlAm ligands are exchanged for oleic acid (OlAc). NMR data show that the OlAc ligands are tightly bound to the Qdot surface, with a coverage of 3.0±0.4 nm(-2). In addition, we demonstrate that they are bound as oleate ions. Combining this with the inorganic Qdot composition, we observe that charge-neutral Qdots are obtained when taking into account the charge of the stoichiometric PbS Qdot core, the surface excess of Pb ions, the surface-adsorbed Cl ions and the oleate ligands. The Qdot suspensions are stable under atmospheric conditions, showing no changes in the NMR and absorbance spectra for several weeks. Finally, we determine the photoluminescence quantum yield (PL QY) for OlAc-capped PbS Qdots, synthesized either with or without TOP. In both cases, they are highly luminescent, with PL QY values varying between 20 and 90%, depending on the Qdot size.

Solubilization of flurbiprofen within non-ionic Tween 20 surfactant micelles: a 19F and 1H NMR study.

The solubilization of the poorly water soluble anti-inflammatory drug flurbiprofen in non-ionic Tween 20 surfactant micellar solutions was studied by both (19)F and (1)H NMR spectroscopy in an acidic environment. These non-destructive techniques allowed us to investigate the effect of temperature cycling in situ. Using (19)F NMR, an increased solubilisation capacity was observed as the temperature increased. This effect became more pronounced above the cloud point, which was reduced by more than 30 degrees C in the presence of an excess of flurbiprofen. Upon clouding, peak splitting was observed in the (19)F spectrum, which indicates that two pools of solubilised flurbiprofen exist that are in slow exchange on the NMR frequency timescale. The clouding and solubilization processes were found to be reversible, albeit with slow kinetics. Based on chemical shift differences of both Tween 20 and flurbiprofen, as well as NOESY experiments, the flurbiprofen was found to be accumulated within the palisade layer of the Tween 20 micelles.