Association between the interleukin-1ß C-511T polymorphism and periodontitis: a meta-analysis in the Chinese population.

The association between the interleukin-1 beta (IL-1ß) C-511T (or rs16944) polymorphism and periodontitis remains inconclusive, even though there have been previous studies on this association. To assess the effects of IL-1ß C-511T variants on the risk of development of periodontitis, a meta-analysis was performed in a single ethnic population. Studies, published up to December 2015, were selected for the meta-analysis from PubMed and Chinese databases. The associations were assessed with pooled OR and 95%CI. This meta-analysis identified 8 studies, including 1276 periodontitis cases and 1558 controls. Overall, a significant association between the IL-1ß C-511T polymorphism and periodontitis was found in the Chinese population (TT vs CC: OR = 1.48, 95%CI = 1.19-1.85; TT + CT vs CC: OR = 1.50, 95%CI = 1.25-1.81; T vs C: OR = 1.33, 95%CI = 1.06-1.68). In the subgroup analyses based on geographical area(s), source of controls, and type of periodontitis, significant results were obtained for the association between IL-1ß C-511T variants and periodontitis. Our meta-analysis indicated that the IL-1ß C-511T polymorphism may be a genetic susceptibility factor for periodontitis in the Chinese population. This marker could be used to identify Chinese individuals at a high risk for periodontitis.

Isolation and characterization of a bacterial strain of the genus Ochrobactrum with methyl parathion mineralizing activity.

AIMS: To isolate and characterize a methyl parathion (MP)-mineralizing bacterium, and to elucidate the degradative pathway of MP and localize the responsible degrading genes. METHODS AND RESULTS: A bacterial strain, designated B2, capable of mineralizing MP was isolated from the MP-polluted soil. Analysis of the 16S rRNA gene sequence and phenotypic analysis suggested that strain B2 had a close relationship with Ochrobactrum anthropi. B2 could totally degrade MP and four metabolites [p-nitrophenol (PNP), 4-nitrocatechol (4-NC), 1,2,4-benzenetriol (BT) and hydroquinone (HQ)] were identified by HPLC and gas chromatography-mass spectrometry analyses. Plasmid curing of strain B2 resulted in the loss of ability of B2 to degrade PNP, but not the ability to hydrolyse MP. CONCLUSIONS: Ochrobactrum sp. B2 can mineralize MP rapidly via PNP, 4-NC, BT and HQ pathway. B2 harbours a plasmid encoding the ability to degrade PNP, while MP-hydrolysing activity is encoded on the bacterial chromosome. SIGNIFICANCE AND IMPACT OF THE STUDY: This new bacterial strain (B2) capable of mineralizing MP will be useful in a pure-culture remediation process of organophosphate pesticides and their metabolites such as nitroaromatics.

[The efficacy of parenteral nutrition with different energy for systemic inflammatory response syndrome (SIRS) in the aged].

To evaluate the efficacy and safety of parenteral nutrition (PN) with lower or higher energy for treating systemic inflammatory response syndrome (SIRS) in the aged, thirty old SIRS critically ill patients were involved in a randomized control clinical trial. Fifteen cases were treated by PN with lower energy (105 KJ.kg-1.d-1), and another fifteen cases with higher energy (146 KJ.kg-1.d-1) for 6 days. The data about metabolisms of protein, glucose and fat, blood gas analysis, acute physiology and chronic health evaluation (APACHE II) were analysed. The results showed that: 1. The effects of 105 KJ.kg-1.d-1 PN in increasing organism protein and maintaining nitrogen balance were the same as those of 146 KJ.kg-1.d-1 PN. 2. The levels of fast blood sugar and triglyceride maintained unchanged in 105 KJ.kg-1.d-1 PN group, while they increased significantly in 146 KJ.kg-1.d-1 PN group(P < 0.05). 3. PaO2 was raised greatly in 105 KJ.kg-1.d-1 PN group, while not in 146 KJ.kg-1.d-1 PN group. 4. 105 KJ.kg-1.d-1 PN and 146 KJ.kg-1.d-1 PN decreased APACHE II(P < 0.05), but the efficiency of 105 KJ.kg-1.d-1 PN was better than that of 146 KJ.kg-1.d-1 PN (P < 0.05). We conclude that PN with 105 KJ.kg-1.d-1 energy intake is more effective and safer for treating SIRS than that with 146 KJ.kg-1.d-1 in the aged.

Preparation of antibodies directed to the Babesia ovata- or Theileria sergenti-parasitized erythrocytes.

To investigate the surface antigens of the bovine red blood cells (RBCs) parasitized by Babesia ovata or Theileria sergenti, attempts were made to produce monoclonal antibodies (mAbs) with BALB/c mice. Comparable numbers of hybridomas producing anti-piroplasm mAbs, as well as anti-bovine RBC mAbs, were obtained from the mice immunized with B. ovata- or T. sergenti-PRBCs. However, mAbs directed to the surface of parasitized RBCs (PRBCs) were obtained only from the mice immunized with B. ovata-PRBCs, but not from those immunized with T. sergenti-PRBCs. When serum samples from the immunized mice and the infected cattle were examined, antibodies recognizing B. ovata-PRBC surface were detected in the sera against B. ovata, but analogous antibodies were undetectable in the sera against T. sergenti, despite that the sera showed substantial antibody titers to T. sergenti piroplasms. The results suggest that significant antigenic modifications occur on the surface of B. ovata-PRBCs, but not on the surface of T. sergenti-PRBCs.