RESUMO
To explore the effect of Internet+diet self-management intervention technology on the blood pressure control of hypertension high-risk population through the intervention of hypertension high-risk population in Haikou City community, so as to provide scientific evidence for the prevention and treatment of cardiovascular diseases (CVD). The multi-stage cluster sampling method was used, and 295 hypertension high-risk participants were recruited from 15 communities in Haikou City from July to December 2021. The 15 communities were randomly divided into three groups: blank group, traditional group and Internet plus group by random number table method. The blank group referred to the group (99 participants) that did not take special intervention measures but the routine interventions in accordance with the "National Basic Public Health Service Standards (the Third Edition) Health Education Service Standards". On the basis of the blank group, the traditional group (95 participants) was intervened by giving additional traditional methods such as holding lectures and distributing popular science books. The Internet plus group (101 participants) was given additional Internet measures on the basis of the intervention of the traditional group. After 6 months, questionnaires, laboratory biochemical tests, and physical measurements were conducted. SPSS 25.0 software was applied for data analysis. Measurement data that followed normal distribution were statistically described by using mean±standard deviation, analysis of variance was used for inter group comparisons before intervention, analysis of covariance was used for inter group comparisons after intervention, and Bonferroni adjustment was used for pairwise comparisons between groups. Measurement data that did not follow the Normal distribution were represented by M (Q1, Q3). The rank sum test was used for inter group comparison. The k sample Kruskal Wallis single factor ANOVA was used to compare the distribution between different groups. Counting data were described by composition ratio or rate. Under the premise of balanced comparison between groups before intervention, Chi-squared test was used for inter group comparison after intervention, and Bonferroni adjustment method was used for pairwise comparison between groups. The results showed that a total of 295 participants were included, with males accounting for 35.6% (105) and females accounting for 64.4% (190). The age ranged from 55 to 74 years old, with an average age of (64.69±5.73) years. The number of married accounted for 95.6% (282 participants). There were no statistically significant differences in gender, age, family history, education level, occupation, marital status, drinking habits, regular exercise, dietary status, SBP (systolic blood pressure), DBP (diastolic blood pressure), pulse pressure difference, BMI (body mass index), folic acid, and 24-hour urine sodium among the three groups upon enrollment (P values>0.05). After the intervention, the drinking rate was as follows: Internet plus group (29, 28.7%)Assuntos
Doenças Cardiovasculares
, Hipertensão
, Autogestão
, Masculino
, Feminino
, Humanos
, Pessoa de Meia-Idade
, Idoso
, Pressão Sanguínea
, Hipertensão/epidemiologia
, Hipertensão/prevenção & controle
, Doenças Cardiovasculares/prevenção & controle
, Dieta
, Sódio
, Internet
, Ácido Fólico
RESUMO
Objective: To investigate the efficacy and safety of a new cervical artificial disc prosthesis in the treatment of cervical degenerative diseases. Methods: The clinical data of 18 patients with single-level cervical degenerative diseases who underwent three dimensional printed anatomical bionic cervical disc replacement at Department of Spinal Surgery,Honghui Hospital,Xi'an Jiaotong University from May 2019 to July 2020 were analyzed retrospectively. There were 7 males and 11 females,aged (45±8) years old(range:28 to 58 years).The surgical segment was located at C3ï¼4 level in 2 cases, C4ï¼5 level in 5 cases, C5ï¼6 level in 9 cases, and C6ï¼7 level in 2 cases.The clinical and radiographic outcomes were recorded and compared at preoperative,postoperative times of one month and twelve months.The clinical assessments contained Japanese orthopedic association (JOA) score,neck disability index (NDI) and visual analogue scale (VAS).Imaging assessments included range of motion (ROM) of cervical spine, prosthesis subsidence and prosthesis anteroposterior migration.Repeated measurement variance analysis was used for comparison between groups,and paired t test was used for pairwise comparison. Results: All patients underwent the operation successfully and were followed up for more than 12 months.Compared with preoperative score,the JOA score,NDI and VAS were significantly improved after surgery (all P<0.01).There was no significant difference in postoperative ROM compared with 1-and 12-month preoperative ROM (t=1.570,P=0.135;t=1.744,P=0.099). The prosthesis subsidence was (0.29±0.13) mm (range: 0.18 to 0.50 mm) at 12-month postoperatively.The migration of prosthesis at 12-months postoperatively were (0.71±0.20) mm (range: 0.44 to 1.08 mm).There was no prosthesis subsidence or migration>2 mm at 12-month postoperatively. Conclusion: Three dimensional printed anatomical biomimetic cervical artificial disc replacement has a good early clinical effect in the treatment of cervical degenerative diseases, good mobility can be obtained while maintaining stability.
Assuntos
Degeneração do Disco Intervertebral , Disco Intervertebral , Substituição Total de Disco , Adulto , Biomimética , Vértebras Cervicais/cirurgia , Feminino , Seguimentos , Humanos , Disco Intervertebral/cirurgia , Degeneração do Disco Intervertebral/cirurgia , Masculino , Pessoa de Meia-Idade , Amplitude de Movimento Articular , Estudos Retrospectivos , Substituição Total de Disco/métodos , Resultado do TratamentoRESUMO
OBJECTIVE: This study was designed to investigate the role of microRNA-198 in thyroid cancer (TCa) progression. PATIENTS AND METHODS: Quantitative real-time polymerase chain reaction (qRT-PCR) was carried out to examine microRNA-198 and H3F3A levels in tumor tissue specimens and paracancerous ones collected from 50 patients with TCa, and the interplay between microRNA-198 or H3F3A and some clinical indicators or prognosis of TCa patients was analyzed as well. MicroRNA-198 and H3F3A overexpression models were constructed using lentivirus in TCa cell lines TPC-1 and BHP2-7, and the impacts of microRNA-198 on TCa cell functions were evaluated by using cell counting kit-8 (CCK-8), plate clone formation, and transwell assays. Finally, recovery investigations were conducted to explore the underlying mechanisms as well as the interaction between microRNA-198 and H3F3A. RESULTS: QRT-PCR indicated that in tumor tissues of TCa patients, microRNA-198 showed a remarkably lower expression than in adjacent normal tissue samples. Compared with patients with high expression of microRNA-198, those with microRNA-198 low expression had more advanced tumor stage, larger tumor size, higher lymph node metastasis rate, and lower overall survival rate. Meanwhile, the results of research on H3F3A were just opposite to the above observations on microRNA-198. In in vitro cell experiments, overexpression of microRNA-198 significantly weakened the proliferation and migration ability of thyroid tumor cells. Besides, Luciferase reporter gene experiment revealed that H3F3A was a specific target gene for microRNA-198. Moreover, qRT-PCR indicated that H3F3A and microRNA-198 were negatively correlated in thyroid carcinoma tissues. In addition, compared with NC group, overexpression of H3F3A markedly enhanced the migration and proliferative capacity of TCa cells. Lastly, recovery experiment revealed a mutual regulation between microRNA-198 and H3F3A, the two of which may together participate in the malignant progression of TCa. CONCLUSIONS: MicroRNA-198 is remarkably reduced in TCa and inhibits malignant progression of TCa by regulating H3F3A. Meanwhile, microRNA-198 is remarkably associated with pathological stage, tumor size, lymph node metastasis, and poor prognosis of TCa.
Assuntos
Histonas/metabolismo , MicroRNAs/metabolismo , Neoplasias da Glândula Tireoide/metabolismo , Adulto , Movimento Celular , Proliferação de Células , Células Cultivadas , Feminino , Histonas/genética , Humanos , Masculino , MicroRNAs/genética , Neoplasias da Glândula Tireoide/patologiaRESUMO
Genomic full-length sequence of HLA-B*15:178 was identified by a group-specific sequencing approach from China.
Assuntos
Genômica/métodos , Antígenos HLA-B/genética , Análise de Sequência de DNA/métodos , Doadores de Tecidos , Sequência de Bases , Humanos , Homologia de SequênciaRESUMO
Macdunnoughia crassisigna Warren (Lepidoptera: Noctuidae) is a highly destructive herbivore that poses a serious risk to cotton, maize, soybean, and cruciferous vegetables in East Asia. Examining the effects of various biotic and abiotic factors on the flight performance of M. crassisigna is crucial for a better understanding of its trans-regional migration. In this study, the flight activity of M. crassisignai moths of different ages, under different temperatures and relative humidity (RH) levels, was evaluated by tethering individuals to computerized flight mills for a 24-h trial period. The results showed that M. crassisignai had the capacity for sustained flight and the flight ability was strongest in 3-day-old individuals, and then their flight performance decreased significantly in older moths. For both sexes, temperature had a significant effect on their flight performance, and the flight activity was relatively higher at 24-28°C than other temperatures. There was a significant effect of RH on all flight parameters of the tested moths, and the flight activity was relatively higher at RH of 60-75% than other RH levels. For 3-day-old moths under the optimum conditions (24°C and 75% RH) throughout the 24 h scotophase, their mean flight distance reached 66 km, and the mean flight duration reached 13.5 h, suggesting M. crassisigna possess strong potential to undertake long-distance migration. These findings will be helpful for developing sound forecasting systems of this pest species.
Assuntos
Voo Animal , Mariposas/fisiologia , Fatores Etários , Animais , Feminino , Umidade , Masculino , Fatores Sexuais , TemperaturaRESUMO
HLA-A*24:353 differs from HLA-A*24:02:01 by an amino acid exchange glutamine to glutamate at position 316.
Assuntos
Alelos , Éxons , Antígeno HLA-A24/genética , Polimorfismo de Nucleotídeo Único , Linfócitos T Citotóxicos/imunologia , Substituição de Aminoácidos , Povo Asiático , Sequência de Bases , Clonagem Molecular , Códon/química , Expressão Gênica , Genótipo , Antígeno HLA-A24/imunologia , Hepatite B/imunologia , Hepatite B/virologia , Teste de Histocompatibilidade , Humanos , Reação em Cadeia da Polimerase , Alinhamento de Sequência , Análise de Sequência de DNA , Linfócitos T Citotóxicos/virologiaRESUMO
KIR3DS1*082 allele differs from the closest allele KIR3DS1*01301 at nucleotide 1114T>C in exon 8.
Assuntos
Alelos , Éxons , Polimorfismo de Nucleotídeo Único , Receptores KIR3DS1/genética , Doadores de Tecidos , Substituição de Aminoácidos , Povo Asiático , Sequência de Bases , Transplante de Medula Óssea , Códon/química , Expressão Gênica , Genótipo , Humanos , Tipagem Molecular , Reação em Cadeia da Polimerase , Receptores KIR3DS1/imunologia , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
KIR2DL1*00602 differs from KIR2DL1*00302 by a non-synonymous mutation in exon 7.
Assuntos
Alelos , Éxons , Polimorfismo de Nucleotídeo Único , Receptores KIR2DL1/genética , Substituição de Aminoácidos , Povo Asiático , Sequência de Bases , Clonagem Molecular , Códon/química , Expressão Gênica , Genótipo , Transplante de Células-Tronco Hematopoéticas , Teste de Histocompatibilidade , Humanos , Reação em Cadeia da Polimerase , Receptores KIR2DL1/imunologia , Alinhamento de Sequência , Análise de Sequência de DNA , Doadores de TecidosRESUMO
KIR2DL1*030 differs from KIR2DL1*00302 by a single non-synonymous mutation in exon 4.
Assuntos
Alelos , Éxons , Células Matadoras Naturais/imunologia , Polimorfismo de Nucleotídeo Único , Receptores KIR2DL1/genética , Substituição de Aminoácidos , Povo Asiático , Sequência de Bases , Clonagem Molecular , Códon/química , Expressão Gênica , Genótipo , Teste de Histocompatibilidade , Humanos , Células Matadoras Naturais/citologia , Reação em Cadeia da Polimerase , Cultura Primária de Células , Receptores KIR2DL1/imunologia , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
Polyhydroxyalkanoates (PHAs) are a class of natural polyesters as carbon and energy reserves by >300 species of microorganisms. They are fully biodegradable, biocompatible and piezoelectric biopolymers that have attracted much attention recently as the biomaterial of choice for medical applications. However, the toughness, processability and hydrophilicity of PHAs need to tune to expand their applications as tissue engineering scaffolds or drug delivery systems. Reactive polymer blending is one of the most economic and versatile way to produce materials combining the desired properties via forming the compatibilizing agents in situ or inducing the chemico-physical interactions between polymer blends. This review focuses on the PHAs-based reactive blends aiming to present a brief introduction to the mechanism of reactive polymer blending technique, including the formation of H-bonding, branching/crosslinking copolymers, graft copolymers or complex copolymers during polymer blending process.
Assuntos
Tecnologia Biomédica/métodos , Poli-Hidroxialcanoatos/química , Poli-Hidroxialcanoatos/síntese química , Animais , Sistemas de Liberação de Medicamentos , Humanos , Engenharia Tecidual , Alicerces Teciduais/químicaRESUMO
HLA-A*24:02:09 shows one nucleotide difference from HLA-A*24:02:01:01 at position 408 in exon 3 (codon 112 GGG>GGC).
Assuntos
Alelos , Antígeno HLA-A24/genética , Mutação Puntual , Povo Asiático , Sequência de Bases , Clonagem Molecular , Códon , Éxons , Genótipo , Antígeno HLA-A24/imunologia , Teste de Histocompatibilidade , Humanos , Dados de Sequência Molecular , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
The novel HLA-DPA1*01:11 allele differs from the closest allele DPA1*01:03:01 by single nucleotide change at coding sequence nucleotide 242 A>G (codon 50 CAA>CGA) in exon 2.
Assuntos
Alelos , Povo Asiático/genética , Etnicidade/genética , Cadeias alfa de HLA-DP/genética , Teste de Histocompatibilidade , Análise de Sequência de DNA , Sequência de Bases , Humanos , Dados de Sequência Molecular , Alinhamento de SequênciaRESUMO
Compared with HLA-DPB1*24:01, DPB1*139:01 has two changes at nucleotide positions 280 (A to C) and 338 (G to T).
Assuntos
Cadeias beta de HLA-DP/genética , Polimorfismo de Nucleotídeo Único , Alelos , Substituição de Aminoácidos , Povo Asiático , Sequência de Bases , Transplante de Medula Óssea , Clonagem Molecular , Éxons , Cadeias beta de HLA-DP/imunologia , Haplótipos , Teste de Histocompatibilidade , Humanos , Íntrons , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Alinhamento de Sequência , Análise de Sequência de DNARESUMO
The objective of this study was to investigate the pharmacokinetics and bioavailability of valnemulin in broiler chickens after intravenous (i.v.), intramuscular (i.m.) and oral administrations of 10 mg/kg body weight (bw). Plasma samples were analyzed by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Pharmacokinetic characterization was performed by non-compartmental analysis using WinNonlin program. After intravenous administration, distribution was wide with the volume of distribution based on terminal phase(V(z)) of 4.27 ± 0.99 Lâ/kg. Mean valnemulin t(1/2ß)(h), Cl(ß)(Lâ/hâ/kg), V(ss)(Lâ/kg) and AUC((0-∞))(µg·hâ/mL) values were 2.85, 0.99, 2.72 and 10.34, respectively. After intramuscular administration, valnemulin was rapidly absorbed with a C(max) of 2.2 µg/mL achieved at 0.43 h (t(max)), and the absolute bioavailability (F) was 88.81%; and for the oral route the same parameters were 0.66 ± 0.15 µg/mL, 1.54 ± 0.27 h and 74.42%. A multiple-peak phenomenon was present after oral administration. The plasma profile of valnemulin exhibited a secondary peak during 2-6 h and a tertiary peak at 32 h. The favorable PK behavior, such as the wide distribution, slow elimination and acceptable bioavailability indicated that it is likely to be effective in chickens.
Assuntos
Antibacterianos/farmacocinética , Galinhas/metabolismo , Administração Oral , Animais , Antibacterianos/administração & dosagem , Disponibilidade Biológica , Cromatografia Líquida de Alta Pressão/veterinária , Diterpenos/administração & dosagem , Diterpenos/farmacocinética , Esquema de Medicação/veterinária , Injeções Intramusculares/veterinária , Injeções Intravenosas/veterinária , Distribuição Aleatória , Espectrometria de Massas em Tandem/veterináriaAssuntos
Imunossupressores/uso terapêutico , Fator Estimulador de Colônias de Macrófagos/sangue , Espondilite Anquilosante/tratamento farmacológico , Talidomida/uso terapêutico , Fator de Crescimento Transformador beta1/sangue , Adolescente , Adulto , Biomarcadores/sangue , Criança , Humanos , Masculino , Projetos Piloto , Adulto JovemRESUMO
The feasibility of low energy processing in ocular tissues with femtosecond laser sources was investigated in this research. One laser source was a femtosecond amplifier, and the other was a femtosecond oscillator. The amplifier used in this experiment was a CPA-2001 (Clark-MXR, Inc), with 150 fs pulse duration and 1 kHz repetition rate. The femtosecond oscillator (model 900-B Mira) produced a 200 fs pulse duration and a 76 MHz repetition rate. Both these two laser systems operated at 800 nm wavelengths. Firstly, the pulse intensity thresholds in water produced by the two laser sources were compared. The optical breakdown probability analysis shows that the pulse energy threshold achieved by the oscillator was less than 10% of that achieved by the amplifier. Then, the non-linear propagation of the femtosecond pulses in the ocular tissues was studied with the femtosecond oscillator. The results showed a potential for pulse energy processing at the nanojoule level with a femtosecond oscillator in glaucoma treatment.
Assuntos
Córnea/efeitos da radiação , Glaucoma/radioterapia , Lasers de Estado Sólido/uso terapêutico , Terapia com Luz de Baixa Intensidade/instrumentação , Amplificadores Eletrônicos , Humanos , Modelos Biológicos , Espalhamento de Radiação , Processamento de Sinais Assistido por ComputadorRESUMO
Agaricus blazei Murill is an edible fungus used in traditional medicine, which has various well-documented medicinal properties. In the present study, we investigated the effects of hemicellulase-derived mycelia extract (Agaricus blazei fraction H: ABH) on the immune system. First, we examined the cytokine-inducing activity of ABH on human peripheral mononuclear cells (PBMC). The results indicated that ABH induced expression of IL-12, a cytokine known to be a critical regulator of cellular immune responses. Flow cytometric analysis demonstrated the induction of IL-12 production by the CD14-positive cell population, consisting of monocytes/macrophages (Mo/Mphi). Furthermore, the elimination of Mo/Mphi attenuated IL-12 production in PBMC. ABH-induced IL-12 production was inhibited by anti-CD14 and anti-TLR4 antibodies but not by anti-TLR2 antibody. The activity of ABH was not inhibited by polymyxin B, while the activity of lipopolysaccharide used as a reference was inhibited. Oral administration of ABH enhanced natural killer (NK) activity in the spleen. These findings suggest that ABH activated Mo/Mphi in a manner dependent on CD14/TLR4 and NK activity.
RESUMO
Corticosterone may nongenomically affect cell functions in addition to its well-characterized effects on gene expression. The purpose of this study is to examine if corticosterone has a rapid nongenomic effect on excitability of dorsal root ganglion neurons by using patch-clamp and single-cell Ca(2+) microfluometry techniques. The results show that corticosterone has a dose-dependent rapid inhibitory effect on the voltage-dependent calcium currents in dorsal root ganglion neurons. Moreover, corticosterone inhibits [Ca(2+)](i) elevation induced by 50 mM high K(+) within just 3 s. The inhibitory effects of corticosterone on the voltage-dependent calcium current and high K(+)-induced calcium influx diminish after adding protein kinase C inhibitor or pretreatment with pertussis toxin for 24 h. Our results demonstrate an nongenomic effect of corticosterone on the excitability of dorsal root ganglion neurons and the effect is mediated through a putative pertussis toxin-sensitive G-protein-coupled receptor and activation of protein kinase C.