Serum protein albumin and chromium: Mechanistic insights into the interaction between ions, nanoparticles, and protein.

The interaction of human proteins and metal species, both ions and nanoparticles, is poorly understood despite their growing importance. These materials are the by-products of corrosion processes and are of relevance for food and drug manufacturing, nanomedicine, and biomedical implant corrosion. Here, we study the interaction of Cr(III) ions and chromium oxide nanoparticles with bovine serum albumin in physiological conditions. This study combined electrophoretic mobility measurements, spectroscopy, and time-of-flight secondary ion mass spectrometry with principal component analysis. It was determined that neither metal species resulted in global albumin unfolding. The Cr(III) ions interacted strongly with amino acids found in previously discovered metal binding sites, but also were most strongly implicated in the interaction with negatively charged acid residues, suggesting an electrostatic interaction. Bovine serum albumin (BSA) was found to bind to the Cr2O3 nanoparticles in a preferential orientation, due to electrostatic interactions between the positive amino acid residues and the negative chromium oxide nanoparticle surface. These findings ameliorate our understanding of the interaction between trivalent chromium ions and nanoparticles, and biological macromolecules.

Serum Albumin Aggregation Facilitated by Cobalt and Chromium Metal Ions.

The interaction of serum proteins with cobalt (Co) and chromium (Cr) ions is poorly understood, but it is suspected to result in protein aggregation, which may alter the corrosion process of biomedical CoCr alloys or result in adverse health effects. Here, we study the aggregation ability and mechanism of bovine serum albumin (BSA) induced or accelerated by aqueous Co(II) and Cr(III) ions. The metal salts were selected by chemical speciation modeling, and they did not affect the pH or precipitate under simulated physiological conditions (150 mM NaCl and pH 7.3). The counterion of Cr(III) influenced the binding to BSA only at physiologically irrelevant low ionic strength. This study used a variety of spectroscopic and light scattering methods. It was determined that both metal ions and an equimolar mixture of metal ions have the potential to induce protein aggregation. Melting curves collected by circular dichroism spectroscopy indicate that Co(II) significantly reduced BSA's melting temperature when compared with Cr(III) or an equimolar mixture of Co(II) and Cr(III), both of which increased the melting temperature of BSA. The metal ions in solution preferentially interacted with BSA, resulting in the depletion of metal ions from the surrounding protein-free solution. Finally, this study suggests that the likely mechanism for Co(II)- and Cr(III)-induced BSA aggregation is salt bridging between protein molecules.

Comparison of different surface disinfection treatments of drinking water facilities from a corrosion and environmental perspective.

Surface disinfection of water facilities such as water wells requires measures that can remove pathogens from the walls to ensure a high drinking water quality, but many of these measures might increase corrosion of the contact surfaces (often highly pure steel) and affect the environment negatively due to disinfectant-contaminated waste sludge and wastewater. Today, most treatments worldwide are based on hypochlorites. We investigated the extent of corrosion during treatments of steel at relevant conditions of ozone, sodium, and calcium hypochlorite for drinking water preparation, utilizing weight loss, electrochemical, solution analytical, and surface analytical methods. The ozone treatment caused significantly less corrosion as compared with sodium or calcium hypochlorite with 150-250 mg/L active chlorine. Hypochlorite or other chlorine-containing compounds were trapped in corrosion products after the surface disinfection treatment with hypochlorite, and this risked influencing subsequent corrosion after the surface disinfection treatment. A life cycle impact assessment suggested ozone treatment to have the lowest negative effects on human health, ecosystems, and resources. Calcium hypochlorite showed the highest negative environmental impact due to its production phase. Our study suggests that ozone surface disinfection treatments are preferable as compared with hypochlorite treatments from corrosion, economic, and environmental perspectives.