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1.
Artigo em Coreano | WPRIM (Pacífico Ocidental) | ID: wpr-1043347

RESUMO

Purpose@#This study aimed to identify the relationship between nursing professionalism and nursing intention for patients with emerging infectious diseases of nursing students who had experienced coronavirus disease 2019 (COVID-19), with a focus on the mediating effect of e-Health literacy. @*Methods@#The study surveyed 177 nursing students who had experienced COVID-19. The data were collected using self-reported questionnaires. The collected data were analyzed using IBM SPSS statistics 25.0, and the mediating effect was analyzed through the SPSS Process macro model 4. @*Results@#Nursing professionalism (β=.26, p=.002) and e-Health literacy (β=.18, p=.021) were found to be significant predictors of nursing intention for patients with emerging infectious diseases. In addition, e-Health literacy partially mediated the relationship between nursing professionalism and nursing intention for patients with emerging infectious diseases. @*Conclusion@#e-Health literacy was a mediating factor in the relationship between the nursing professionalism and nursing intention of nursing students for patients with emerging infectious diseases. In order to improve nursing intention of nursing students for patients with emerging infectious diseases, it is important to develop an education program that can enhance their e-Health literacy as well as nursing professionalism.

2.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-1043406

RESUMO

Purpose@#This study aimed to develop a counseling program called Motivational Interviewing-Cognitive Behavioral Therapy Integrated Counseling Program (MICBT-CP) and evaluate its effectiveness in post-case management of individuals who have attempted suicide. @*Methods@#Twenty-eight participants with a history of suicide attempts and high scores on Beck’s Scale for Suicidal Ideation were divided into three groups: an experimental group (EG), control group 1 (CG1), and control group 2 (CG2). Each group received different interventions: MICBT-CP for the EG, cognitive-behavioral treatment-based case management (CBT-CM) for CG1, and general case management (GCM) for CG2. Measurements of suicidal ideation, depression, hopelessness, problem-solving competence, life motivation, and perceived motivation were taken at baseline (T1), 6 weeks (T2), and 18 weeks (T3). @*Results@#Significant improvements in life motivation, perceived motivation, and problem-solving competence were observed in the EG compared to CG1 and CG2. Moreover, the EG showed significant reductions in suicidal ideation, hopelessness, and depression. The treatment of CG2 with GCM did not yield significant results, indicating the limitations of current GCM in post-suicide attempt case management. @*Conclusion@#The study emphasizes the need for effective programs such as MICBT-CP and improved practitioner skills in post-suicide case management.

3.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-1002142

RESUMO

The purpose of this study is to evaluate the effect of photo-polymerization on the mechanical strength of the hybrid resin block. Hybrid composite resin was fabricated by polymer matrix mixed with inorganic filler and various additive. Visible light (460 nm) LED curing device for photo-polymerization was designed and fabricated. Photo-polymerization of resin block was performed under 3 hrs at 1200 mW/cm2 , 2 hrs at 1600 mW/cm2 , 3 hrs at 1600 mW/cm2 , and 4 hrs at 1600 mW/cm2 of irradiation intensities and time. Mechanical strength was measured using flexural strength. In addition, the sample treated for 2 hrs at 1600 mW/cm2 was performed post-treatment under conditions of plasma surface treatment (100 W, 5 min) and light curing for 15 min in order to improve the degree of polymerization. And then specimen was measured for flexural strength again. After the photo-polymerization, surface morphology was analyzed by SEM. The flexural strength of the composite resin block was significantly increased as the irradiation time was increased. Especially, post-treated sample showed higher flexural strength than that of the other samples. In the SEM analysis, surface morphology was no significant difference regardless of the photo-polymerization conditions. Light irradiation and plasma treatment could be a powerful technique to improve the polymerization of hybrid composite resin block for CAD/CAM-processed prosthesis.

4.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-1041505

RESUMO

Background@#Alloantibodies against red blood cells (RBC) found in pregnant women can cause hemolytic disease in the fetus and newborn (HDFN). Therefore, checking and carefully observing the mother’s unexpected alloantibodies is essential during pregnancy. This study examined the frequency of unexpected antibodies according to the department. @*Methods@#For patients who visited the authors’ hospital from December 31, 2020 to May 1, 2023 the results of RBC antibody screening and unexpected antibody identification tests were collected and classified according to the department. The antibody screening test was detected with Qwalys-3 DIAGAST, Loos Cedex, France) equipment using ABS HEMASCREEN (DIAGAST), and the antibody identification test was performed using the Resolve panel (Ortho-Clinical Diagnostics, San Diego, USA). The difference in frequency of each antibody according to the patient group was tested using Pearson’s chi-square test and Fisher’s exact test according to the relative frequency. @*Results@#Among 46,193 patients who underwent unexpected antibody screening, 9,531 were obstetrics, and 18,313 were infertility centers. One hundred and seventy-seven patients underwent the unexpected antibody identification test: 57 obstetrics patients, 42 infertility center patients, and 78 positive patients who visited other departments.One hundred and ninety-three antibodies were identified, and there was no significant difference in the positive rate of unexpected antibodies by department. The antibody identified with the highest frequency was anti-M (18.1%) followed by anti-E (13.5%). @*Conclusion@#In the East Asian population, anti-M is frequently reported, causing clinical problems. Anti-M was also commonly observed in this study; so, the clinical features should be carefully observed if anti-M is identified.

5.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-1041543

RESUMO

The purpose of this study is to investigate the effect of polishing procedure and pH on the color change of denture base resin. Heat-cured denture resin specimens (10 x 10 x 4 mm) were prepared with three surface roughness levels:G0 (non-polished), G1 (polished with acrylic carbide bur, silicone rubber points and brush wheel), G2 (additional polishing and buffing with lathe, pumice and rouge compound). Each specimen of G0, G1, G2 was immersed in a staining solution with four different acidic conditions (pH 2, pH 4, pH7, pH 9.18) for 14 days. Surface roughness (Ra) was measured with AFM (atomic force microscope) and the surface was inspected with a digital microscope. Colorchange (∆E*) was quantified using a spectrophotometer in CIE Lab color space. After immersion, inspection with the digital microscope showed most stain in G0, followed by G2 and G1. G0 have shown significantly higher color change (∆E*) after immersion than G1 and G2 in different pH solutions other than pH 9.18. In the results of the color change estimation in each group with different pH solutions, all groups showed the least color change in pH 9.18 solution.The highest color change after immersion was observed in G0, pH 7.0 solution and G0, pH 2.0 solution (P0.05). Denture base resin specimen with higher surface roughness showed more significant color change after immersion compared to specimen with lower surface roughness. More significant color changes were observed after immersion in acidic and neutral pH solution than in alkaline solution.

6.
Yonsei Medical Journal ; : 448-454, 2023.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-1003225

RESUMO

Purpose@#The aim of this study was to investigate the relationship between pain duration and pain relief after epidural adhesiolysis. @*Materials and Methods@#Patients with low back pain who underwent lumbar epidural adhesiolysis were enrolled. A clinically significant reduction in pain score was defined as a ≥30% reduction at 6-month follow-up evaluation. Variables were compared based on pain duration categories. Changes in pain scores and pain outcome were also compared. Logistic regression analysis was conducted to identify factors associated with pain relief after adhesiolysis. @*Results@#A total of 169 patients, including 77 (45.6%) patients with a favorable pain outcome, were included for analysis. Patients with a pain duration ≥3 years reported lower baseline pain scores and showed more frequent severe central stenosis. Pain scores significantly decreased over time after the procedure except in patients with a pain duration ≥3 years. Most patients who experienced pain for ≥3 years showed poor pain relief (80.8%), unlike other pain duration categories (pain duration <3 months=48.1%, 3 months–1 year=51.8%, 1–3 years=48.6%). A pain duration ≥3 years and lower baseline pain score were independent factors associated with an unfavorable pain outcome. @*Conclusion@#Pain lasting ≥3 years prior to lumbar epidural adhesiolysis was associated with worse outcomes in terms of pain relief. Therefore, this intervention should be considered early before pain chronification in patients with low back pain.

7.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-967283

RESUMO

Purpose@#This study aims to examine the mediating effects of psychological contracts and subjective career success and the effect of organizational justice on organizational citizenship behavior among hospital nurses. @*Methods@#The data were collected using structured questionnaires through online surveys from 200 nurses in hospitals located in P metropolitan city and J city from February 9 to March 8, 2022. Data were analyzed using SPSS 26.0, Hayes’s SPSS Process Macro 4.0 version program, and bootstrapping. @*Results@#The paths of organizational justice to psychological contract, psychological contract to subjective career success, and subjective career success to organizational citizenship behavior were significant. The double mediating effects of psychological contract and subjective career success were significant in the relationship between organizational justice and organizational citizenship behavior. @*Conclusion@#Based on this study, nursing managers should enable nurses to positively perceive organizational justice and the psychological contract. For this, efforts should be made to secure a transparent and fair system. For subjective career success, it is necessary to establish a career management system and provide opportunities for career development such as relevant education at the organizational level. This will be a motivating process that can induce organizational citizenship behavior.

8.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-967445

RESUMO

Background@#Biogenetic causal explanations of mental disorders are commonly used for public education and campaigns. However, the influence of biogenetic explanations on the ideas about and attitudes toward mental illness is unclear. We examined the influence of biogenetic explanations on attitudes toward mental disorders using a meta-analytic method accompanied by subgroup analyses. @*Methods@#The protocol for this meta-analysis was registered in PROSPERO (CRD42020158656) in March 2020. Experimental and correlational studies were analyzed separately. Five outcome measures related to attitudes toward mental disorders were included: ‘attitude toward helpseeking,’ ‘blame,’ ‘perceived dangerousness,’ ‘prognostic pessimism,’ and ‘social distance.’ Subgroup analyses were performed for the type of mental disorder, population, and geographic region for which a biogenetic explanation was provided. @*Results@#A total of 44 studies were included, of which 24 were experimental and 20 were correlational. A positive attitude toward help-seeking was associated with having a biogenetic concept (d = 0.43; 95% confidence interval [CI], 0.18 to 0.67; P < 0.001) in general population and in Eastern countries in particular. Although a biogenetic explanation was associated with a decreased level of blame (d = −0.20; 95% CI, −0.38 to −0.02; P = 0.029) in the general population, it was also associated with significantly higher levels of perceived dangerousness (d = 0.13; 95% CI, 0.03 to 0.23; P = 0.008). A tendency toward a higher level of prognostic pessimism and social distance was associated with a biogenetic concept of mental disorders although there was no statistical significance. @*Conclusion@#Having a biogenetic concept of the cause of mental disorders was related with a positive attitude toward help-seeking, particularly in the general population and individuals living in Eastern countries. Providing a biogenetic explanation decreased blame toward individuals with mental illness but was associated with increased perceived dangerousness and prognostic pessimism. Therefore, although a biogenetic explanation use of mental health services, it should be carefully applied to avoid an increase in negative thoughts, such as that mental illness is biologically irreversible and untreatable.promotes public

9.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-977183

RESUMO

Background@#A precise anatomical understanding of the adductor canal (AC) and its neural components is essential for discerning the action mechanism of the AC block. We therefore aimed to clarify the detailed anatomy of the AC using micro-computed tomography (micro-CT), histological evaluation, and immunofluorescence (IF) assays. @*Methods@#Gross dissections of 39 thighs provided morphometric data relevant to injection landmarks. Serial sectional images of the AC were defined using micro-CT and ultrasonography. The fascial and neural structures of the AC proper were histologically evaluated using Masson’s trichrome and Verhoeff-Van Gieson staining, and double IF staining using choline acetyltransferase (ChAT) and neurofilament 200 antibodies. @*Results@#The posteromedial branch insertion of the nerve to vastus medialis (NVM) into the lateral border of the AC proper was lower (14.5 ± 2.4 cm [mean ± SD] above the base of the patella) than the origin of the proximal AC. The AC consists of a thin subsartorial fascia in the proximal region and a thick aponeurosis-like vastoadductor membrane in the distal region. In the proximal AC, the posteromedial branch of the NVM (pmNVM) consistently contained both sensory and motor fibers, and more ChAT-positive fibers were observed than in the saphenous nerve (27.5 ± 11.2 / 104 vs. 4.2 ± 2.6 / 104 [counts/µm2], P < 0.001). @*Conclusions@#Anatomical differences in fascial structures between the proximal and distal AC and a mixed neural component of the neighboring pmNVM have been visualized using micro-CT images, histological evaluation, and IF assays.

10.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-937115

RESUMO

Background@#Intraoperative pulmonary aspiration is a rare but potentially fatal complication associated with various risk factors. Preoperative recognition of these risk factors can prevent aspiration events during general anesthesia or facilitate prompt corrective measures in patients experiencing this complication. Case: A 70-year-old female patient with hypertension underwent bilateral total knee arthroplasty under general anesthesia. Despite using a midnight nothing-per-oral (NPO) protocol, an unpredictable intraoperative aspiration event occurred during anesthesia induction. A detailed evaluation of the patient’s medical history and subsequent diagnostic imaging examinations indicated achalasia. She was treated for aspiration pneumonia for 2 weeks. After 2 months, rescheduled total knee arthroplasty was performed under spinal anesthesia without any complications. @*Conclusions@#Obtaining the patient’s medical history and assessing the risk factors are important to prevent unpredictable intraoperative pulmonary aspiration. High-risk patients should undergo adequate preoperative fasting and regional anesthesia or rapid-sequence intubation should be considered for safe induction of general anesthesia.

11.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-926614

RESUMO

Background@#Leprosy is a chronic granulomatous disease caused by Mycobacterium leprae. As leprosy has a long disease course, various dermatoses must be considered in the differential diagnosis. @*Objective@#In Korea, due to the low incidence rate of leprosy and lack of doctors with experience of leprosy, it is difficult to make an early diagnosis. Therefore, we evaluated the importance of cooperation projects in the diagnosis of leprosy. @*Methods@#A retrospective study was performed on 137 patients who were referred by other hospitals to the Institute for Leprosy Research, Korean Hansen Welfare Association, to make an early diagnosis of leprosy from 2009 to 2018. @*Results@#Among 137 patients, 19 were diagnosed with leprosy, which accounted for approximately 37% of new cases of leprosy in Korea during the 10 years. By nationality, 15 were Korean, and 4 were foreigners. The most common type was lepromatous leprosy. All multibacillary patients were positive for phenolic glycolipid-1 antibody, M. leprae-specific gene polymerase chain reaction, and acid-fast bacilli stain. Among the four paucibacillary patients who showed both negative acid-fast bacilli stain and phenolic glycolipid-1, three were positive for polymerase chain reaction. Of the 55 skin biopsy specimens that were not diagnosed with leprosy, granulomatous skin disease was the most commonly diagnosed condition. @*Conclusion@#In this study, we diagnosed patients with leprosy who were referred from other hospitals due to the suspicion of leprosy, which enables early treatment of leprosy. Finally, we believe that our project has public health significance in preventing the spread of leprosy in the community.

12.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-968135

RESUMO

Background@#This study is a 1-year follow-up study for mentally disabled people who started living independently. The purpose of this study is to confirm the performance of the supported independent housing (SIH) program for severe mental illness and to compare the effectiveness with the collective living family, a group home (GH). @*Methods@#This study included mentally disabled people living in publicly supported SIH (n=24) and living in GH (n=31). The outcome measures were brief psychiatric rating scale, Global Assessment Function (GAF), internalized stigma, hope, self-esteem, life satisfaction, and working alliance. Analyzes were performed using descriptive methods and chi-square analysis, Independent t-test, Mann-Whitney U-test, and Friedman test. @*Results@#In the case of the SIH group, GAF and internalized stigma showed a significant effect after 1 year, and the working alliance showed a tendency to decrease at 6 months and then increase again after 1 year. In comparison between the two groups, only GAF showed a significant difference after 1 year. Hope, self-esteem, and life satisfaction showed a significant effect in the GH group after 1 year, but not in the SIH group. @*Conclusions@#Results show that the SIH and GH housing types achieve different results over the 1-year period. GAF and internalized stigma were significant in SIH, and psychological variables were significant in GH. In GAF, SIH showed a more significant effect than GH. Housing support should take into account the various individual circumstances and preferences of the client, and above all, securing housing stability and expanding accessibility is important.

13.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-925338

RESUMO

Purpose@#Extravasation of diagnostic and therapeutic materials might occur when the intravascular solution leaks into the surrounding tissues. Injury associated with extravasation depends on various factors. It may range from mild skin reaction to severe necrosis. However, the incidence rate for extravasation is largely unknown because of the limited reporting in Korea. Therefore, this study was conducted to identify the incidence of extravasation and nurses’ attitude and knowledge of extravasation for providing high-quality nursing care. @*Methods@#Three acute care hospitals were surveyed to estimate the occurrence of extravasation. Knowledge and attitude toward extravasation were investigated from 793 nurses working in six hospitals. @*Results@#The incidence rate of extravasation was 0.5%. Extravasation commonly occurred in elderly patients aged 66 or older (59.9%) and internal medicine (48.2%), and it happened 13.73±20.68 days after hospitalization on average.It mostly occurred in the forearm site (52.9%) and was mainly caused by parenteral nutrition (33.6%). The mean scores of nurses’ knowledge and attitude were 14.63±2.86 and 28.91±36.00, respectively. There was a significant negative correlation between the subjects' knowledge and attitude (r=-.11, p=.002). @*Conclusion@#It is necessary to have a reporting system that can accurately monitor the occurrence of extravasation for patient safety management. In addition, it is necessary to develop a protocol that can be applied to clinical practice and a nurse education program.

14.
Artigo em Coreano | WPRIM (Pacífico Ocidental) | ID: wpr-874710

RESUMO

Purpose@#This study aimed to understand and describe the diabetes self-management experience of visually impaired people with diabetes. @*Methods@#Ten participants were recruited through a website used by the visually impaired from February to March 2020. Data were collected through two focus group interviews conducted in June 2020; each group consisted of five participants. All interviews were recorded with the consent of the participants and transcribed verbatim. The transcribed data were analyzed using qualitative content analysis. @*Results@#Seven categories were as follows; a two-faced, lifelong companion, an unprepared encounter, struggle to live, love-hate relationship with family, strategies to adapt, lessening attention to self-management, the desire to learn properly. @*Conclusion@#It can be concluded that the visually impaired have fewer opportunities for receiving diabetes self-management education than general diabetic patients. Consequently, plans to improve the education available to such patients are required. Additionally, psychological counseling and diabetes education for patients’ families are necessary, and improving the perception of medical workers regarding the visually impaired will be prove useful.

15.
Artigo em Coreano | WPRIM (Pacífico Ocidental) | ID: wpr-920153

RESUMO

Perilymph fistula (PLF) is defined as an abnormal communication between the middle ear cavity and perilymphatic space of the inner ear. Most cases of PLF are responsible for pressure change caused by internal trauma or external trauma. However, spontaneous PLF might occur without an obvious history of barotrauma. We report two cases of possible spontaneous PLF in the postpartum period: both cases involved progressive sensorineural hearing loss with positional dizziness. Although the leakage of perilymph was not found during exploration, both hearing loss and dizziness improved immediately after PLF repair. It is necessary to consider the possibility of PLF in patients who have sudden or progressive sensorineural hearing loss with positional nystagmus. Early surgical exploration is recommended even if the history of barotrauma is not clear.

16.
Artigo em Coreano | WPRIM (Pacífico Ocidental) | ID: wpr-920332

RESUMO

Purpose@#This study was conducted to identify the factors affecting the quality of life among cancer survivors using the results of the 8th National Health and Nutrition Survey. @*Methods@#Data was collected from the KNHANES Ⅷ-1 (2019). The research tool used the demographic and health related characteristics and quality of life (EQ-5D).Among total 8110 participants, 262 cancer survivors were included in the analysis. Data analysis was conducted using SPSS 25 version. @*Results@#The overall mean of quality of life for participants was 0.92±0.13 (range 0~1).There were statistically significant differences in the quality of life results by age (F=6.23, p<.001), marital status (F=7.40 p<.001), education level (F=12.36, p<.001), current economic activity (t=22.28, p<.001), household income (F=5.49, p=.001), cancer type (t=2.61 p=.018), chronic disease (t=21.29, p<.001), health examination (t=5.39, p=.021), cancer screening (t=4.93, p=.027), activity limitation (t=63.93, p<.001), physical activity (F=4.96, p=.008), monthly drinking (t=8.39, p=.004), depressive symptoms (t=13.52, p<.001), private health insurance (t=20.15, p<.001), body mass index (r=-.17 p=.005), subjective health status (r=.42 p<.001), perceived stress level (r=-.18 p=.003). Factors influencing the quality of life of cancer survivors included cancer screening (β=.21, p=003), education level (β=-.22, p=.003), current economic activity (β=.18, p=.010), subjective health status (β=.19, p=.007), depressive symptoms (β=-.15, p=.034), and physical activity (β=.14, p=.037), which explained 24.5% of the quality of life. @*Conclusion@#To improve the quality of life of cancer survivors, the appropriate intervention is needed for effective management considering various factors which affect the quality of life of the cancer survivors.

17.
Health Communication ; (2): 67-74, 2021.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-914415

RESUMO

Background@#: Interprofessional education is a necessary step in preparing healthcare providers. This study was done to develop an interprofessional ethical education for nursing and medical students in South Korea, and examine its effects on their readiness for interprofessional learning and achievement in learning outcomes. @*Methods@#: The study was designed using a one-group pretest-posttest design. The subjects were 34 4th-year nursing students and 39 5th-year medical students who participated in an interprofessional ethical education program. Descriptive statistics, a independent t-test, and a dependent t-test were used for data analysis. @*Results@#: After interprofessional ethical education, students’ readiness for interprofessional learning and achievement in learning outcomes were improved. The improvement of nursing students were higher than that of medical students. @*Conclusion@#: The study results indicate that an interprofessional ethical education for nursing and medical students is effective in increasing their readiness for interprofessional learning and achievement in learning outcomes.

18.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-917588

RESUMO

This study evaluated release of the residual monomer and cytotoxicity after polymerization of three denture base resins (heat curing resin, self-curing resin, light curing resin by 3D printer). Three types of resin specimen according to the manufacturer’s guideline were fabricated: heat curing resin (Group H), self-curing resin (Group S), 3D Printed resin (Group L). Each group of specimen were stored in distilled water at 37 ℃ for 1 day, 1 week, 2 weeks, 3 weeks, 4 weeks, 6 weeks, and 8 weeks in order to measure the release of the residual monomer. Absorbance was measured at each storage time in the three groups. Cytotoxicity is measured by MTT test at each storage time in the three groups. For statistical analysis, one-way ANOVA with post-hoc tests of Dunnett T3 test and Scheffe test were carried out, where p values of less than 0.05 were considered as significant. Group H showed the lower absorbance than Group S and Group L. There was no statistically significant difference in the absorbance of Group S and Group L for 8 weeks, except for the one day. Group H and Group S showed no cytotoxicity, but Group L resulted in cytotoxicity. To conclude, light curing resin used in 3D printer revealed high cytotoxicity in this study. In order to apply the 3D printed denture base resin to clinical situation, it is necessary to develop a method to reduce the release of residual monomers.

19.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-917752

RESUMO

Background@#The purpose of this study was to explore the employment experiences of mentally disabled employees working in the subsidiary company for the disabled. @*Methods@#Ten people who employed in the subsidiary company for the disabled interviewed with 12 months. The study had a qualitative descriptive design and qualitative content analysis was used. @*Results@#Employment experiences of mentally disabled employees was emerged into three domains (support, effort, expectation and demand), six categories (relational support, supportive work environment, system support, self-management efforts, improving the quality of employment, improving awareness) and 12 subcategories. @*Conclusions@#In conclusion, for stable employment of the mentally disabled peoples, efforts are required at the individual, company, and government level. A flexible and careful work environment for the mentally disabled peoples and support systems such as counseling and case management are needed to be considered. Job assignments that meet the individual skills and needs of persons with mental disabilities should be considered, and it is necessary to develop an integrated supported employment program that supports individual skills training after employment. Above all, a government effort is needed to eliminate social prejudice against mental illness, and also an effort to improve employers' awareness are needed.

20.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-785337

RESUMO

PURPOSE: Phosphoinositide 3-kinase (PI3K)-δ-dependent Akt activation is known to play critical roles in various immune responses of white blood cells in which PI3K-δ isoform is mostly expressed in contrast to the classes IA PI3Ks p110α and p110β. However, the immunological role of PI3K-δ isoform is still controversial in airway epithelium under house dust mite (HDM)-induced allergic response. This study aimed to evaluate the role of PI3K-δ isoform in HDM-induced allergic responses, focusing on NLRP3 inflammasome activation in airway epithelium.METHODS: We used wild-type mice and PI3K-δ knock-out (KO) mice for HDM-induced asthma animal model and also performed in vitro experiments using primary cultured murine tracheal epithelial cells and human airway epithelial cells.RESULTS: PI3K-δ activated HDM-induced NLRP3 inflammasome and epithelial cell-derived cytokines in the lung including airway epithelial cells. PI3K-δ KO mice or knock-down of PI3K-δ using siRNA exhibited the significant reduction in allergic asthmatic features and the suppression of NLRP3 inflammasome assembly as well as epithelial cell-derived cytokines. Interestingly, significantly increased expression of PI3K-δ isoform was observed in stimulated airway epithelial cells and the increases in epithelial cell-derived cytokines were markedly suppressed by blocking PI3K-δ, while these cytokine levels were independent of NLRP3 inflammasome activation.CONCLUSIONS: The results of this study suggest that PI3K-δ-isoform can promote HDM-induced allergic airway inflammation via NLRP3 inflammasome-dependent response as well as via NLRP3 inflammasome-independent epithelial cell activation.


Assuntos
Animais , Humanos , Camundongos , Asma , Citocinas , Poeira , Células Epiteliais , Epitélio , Técnicas In Vitro , Inflamassomos , Inflamação , Leucócitos , Pulmão , Modelos Animais , Fosfotransferases , Pyroglyphidae , RNA Interferente Pequeno
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