Neglected role of iron redox cycle in direct interspecies electron transfer in anaerobic methanogenesis: Inspired from biogeochemical processes.

Anaerobic digestion is an indispensable technical option towards green and low-carbon wastewater treatment, with interspecies electron transfer (IET) playing a key role in its efficiency and operational stability. The exogenous semiconductive iron oxides have been proven to effectively enhance IET, while the cognition of the physicochemical-biochemical coupling stimulatory mechanism was circumscribed and remains to be elucidated. In this study, semiconductive iron oxides, α-Fe2O3, γ-Fe2O3, α-FeOOH, and γ-FeOOH were found to significantly enhance syntrophic methanogenesis by 76.39, 72.40, 37.33, and 32.64% through redirecting the dominant IET pathway from classical interspecies hydrogen transfer to robust direct interspecies electron transfer (DIET). Their alternative roles as electron shuttles potentially substituting for c-type cytochromes were conjectured to establish an electron transport matrix associated with conductive pili. Distinguished from the conventional electron conductor mechanism of conductive Fe3O4, semiconductive iron oxides facilitated DIET intrinsically through the capacitive Fe(III/II) redox cycles coupled with secondary mineralization. The growth of Aminobacterium, Sedimentibacter, and Methanothrix was enriched and the gene copy numbers of Geobacteraceae 16S ribosomal ribonucleic acid were selectively flourished by 2.0-∼4.5- fold to establish a favorable microflora for DIET pathway. Metabolic pathways of syntrophic acetogenesis from propionate/butyrate and CO2 reduction methanogenesis were correspondingly promoted. The above findings provide new insights into the underlying mechanism of iron minerals enhancing the DIET-oriented pathway and offer paradigms for redox-mediated energy harvesting biological wastewater treatment.

Interface interaction between silica and organic macromolecule conditioned forward osmosis membranes: Insights into quantitative thermodynamics and dynamics.

Silica scaling is a rising concern in forward osmosis membrane-based water treatment process. The coexistence of ubiquitous organic macromolecules causes complex silica scaling. The silica scaling mechanism on the surface of the organic conditioned membrane remains unclear. An integrated multi scale thermodynamic and dynamic approach was used in this study to provide in-depth insights into the binding effect at the interface between the silica and the organic conditioned membrane at the molecular level. Sodium alginate (SA) was used as the model polysaccharide, bovine serum albumin (BSA) and lysozyme (LYZ) were chosen as two oppositely charged proteins. The results show that the silica scaling degree of different organic conditioned membranes follows the order LYZ > BSA > SA. The binding strength between silica and organic macromolecules and the membrane surface charge are the major factors governing the degree of silica scaling. Quartz crystal microbalance with dissipation (QCM-D), isothermal titration calorimetry (ITC), and extended Derjaguin-Landau-Verwey-Overbeek (XDLVO) model analyses were conducted to quantify the binding capacity of silica to the organic conditioned membrane. The LYZ conditioned membrane exhibits the highest affinity for silica adsorption, and electrostatic interaction was the main molecular interaction force. This study provides fresh insights into how silica and an organic conditioned membrane interact and induce silica scaling, providing new information on potential mechanisms and control strategies to prevent membrane scaling.

Novel electro-assisted micro-aerobic cathode biological technology induces oxidative demethylation of N, N-dimethylformamide for efficient ammonification of refractory membrane-making wastewater.

Recalcitrant and toxicological membrane-making wastewater displays negative impacts on environment, and this is difficult to treat efficiently using conventional hydrolytic acidification. In this study, a novel electro-assisted biological reactor with micro-aerobic cathode (EABR-MAC) was developed to improve the biodegradation and ammonification of N, N-dimethylformamide (DMF) in membrane-making wastewater, and the metabolic mechanism using metagenomic sequencing as comprehensively illustrated. The results showed that EABR-MAC significantly improved the ammonification of refractory organonitrogen and promoted DMF oxidative degradation by driving the electron transferred to the cathode. Additionally, the inhibition rates of oxygen uptake rate and nitrification in EABR-MAC were both lower under different cathode aeration frequency conditions. Microbial community analysis indicated that the functional fermentation bacteria and exoelectrogens, which were correlated with COD removal, ammonification, and detoxification, were significantly enriched upon electrostimulation, and the positive biological connections increased to form highly connected communities instead of competition. The functional genes revealed that EABR-MAC forcefully intervened with the metabolic pathway, so that DMF converted to formamide and ammonia by oxidative demethylation and formamide hydrolysis. The results of this study provide a promising strategy for efficient conversion of organonitrogen into ammonia nitrogen, and offer a new insight into the effects of electrostimulation on microbial metabolism.