Enhancing sweet potato (Ipomoea batatas) resilience grown in cadmium-contaminated saline soil: a synergistic approach using Moringa leaf extract and effective microorganisms application.

Raising soil contamination with cadmium (Cd2+) and salinization necessitates the development of green approaches using bio-elicitors to ensure sustainable crop production and mitigate the detrimental health impacts. Two field trials were carried out to study the individual and combined effects of foliage spraying of Moringa leaf extract (MLE) and soil application of effective microorganisms (EMs) on the physio-biochemical, osmolytes, antioxidants, and performance of sweet potato grown in Cd2+-contaminated salty soil (Cd2+ = 17.42 mg kg-1 soil and soil salinity ECe = 7.42 dS m-1). Application of MLE, EMs, or MLE plus EMs significantly reduced the accumulation of Cd2+ in roots by 55.6%, 50.0%, or 68.1% and in leaves by 31.4%, 27.6%, or 38.0%, respectively, compared to the control. Co-application of MLE and EMs reduced Na+ concentration while substantially raising N, P, K+, and Ca2+ acquisition in the leaves. MLE and EMs-treated plants exhibited higher concentrations of total soluble sugar by 69.6%, free proline by 47.7%, total free amino acids by 29.0%, and protein by 125.7% compared to the control. The enzymatic (SOD, APX, GR, and CAT) and non-enzymatic (phenolic acids, GSH, and AsA) antioxidants increased in plants treated with MLE and/or EMs application. Applying MLE and/or EMs increased the leaf photosynthetic pigment contents, membrane stability, relative water content, water productivity, growth traits, and tuber yield of Cd2+ and salt-stressed sweet potato. Consequently, the integrative application of MLE and EMs achieved the best results exceeding the single treatments recommended in future application to sweet potato in saline soil contaminated with Cd2+.

Application of bio-organic amendments improves soil quality and yield of fennel (Foeniculum vulgare Mill.) plants in saline calcareous soil.

The impact of bio-organic amendments on crop production is poorly understood in saline calcareous soils. The aim in the present study was to determine the effects of the application of organic manure along with lactic acid bacteria (LAB) on soil quality, and morpho-physio-biochemical responses, seed yield (SY) and essential oil yield (EOY) of fennel plants (Foeniculum vulgare Mill.) grown in saline calcareous soils. Eight treatments of farmyard manure (FM) or poultry manure (PM) individually or combined with Lactobacillus plantarum (Lp) and/or Lactococcus lactis (Ll) were applied to saline calcareous soil in two growing seasons. Either FM or PM combined with LAB had beneficial effects on lowering ECe, pH and bulk density and increasing total porosity, organic matter, and water and nutrient retention capacities in addition to total bacterial population in the soil. Growth, nutrient uptake, SY and EOY of plants were also enhanced when fennel seeds were inoculated with Lp and/or Ll and the soil was amended with any of the organic manures under unfavorable conditions. Compared to control (no bio-organic amendments), FM + Lp + Lt or PM + Lp + Lt treatment signficantlly (P ≤ 0.05) increased plant height by 86.2 or 65.0%, total chlorophyll by 73 or 50%, proline by 35 or 45%, glutathione by 100 or 138%, SY by 625 or 463% and EOY by 300 or 335%, respectively, in fennel plants. Co-application of the naturally occurring microorganisms (i.e., LAB) and organically-derived, nutrient-rich fertilizer (i.e., FM or PM) is recommended to improve yield of fennel plants in saline calcareous soils.

Compost and Phosphorus/Potassium-Solubilizing Fungus Effectively Boosted Quinoa's Physio-Biochemical Traits, Nutrient Acquisition, Soil Microbial Community, and Yield and Quality in Normal and Calcareous Soils.

Calcareous soil had sufficient phosphorus and potassium (PK) in different forms due to the high contents of PK-bearing minerals; however, the available PK state was reduced due to its PK-fixation capacity. Compost, coupled with high PK solubilization capacity microbes, is a sustainable solution for bioorganic fertilization of plants grown in calcareous soil. A 2-year field experiment was conducted to investigate the effect of compost (20 t ha-1) with Aspergillus niger through soil drenching (C-AN) along with partial substitution of PK fertilization on quinoa performance in normal and calcareous soils. Treatments included PK100% (72 kg P2O5 ha-1 + 60 kg K2O ha-1 as conventional rate), PK100%+C-AN, PK75%+C-AN, PK50%+C-AN, PK25%+C-AN, and only C-AN in normal and calcareous soils. Results showed that C-AN and reduced PK fertilization (up to 75 or 50%) increased photosynthetic pigments and promoted nutrient acquisition in quinoa grown in calcareous soil. Reduced PK fertilization to 75 or 50% plus C-AN in calcareous soil increased osmoprotectants, nonenzymatic antioxidants, and DPPH scavenging activity of quinoa's leaves compared to the PK0%+C-AN treatment. The integrative application of high PK levels and C-AN enhanced the quinoa's seed nutritional quality (i.e., lipids, carbohydrates, mineral contents, total phenolics, total flavonoids, half maximal inhibitory concentration, and antiradical power) in calcareous soil. At reduced PK fertilization (up to 75 or 50%), application of compost with Aspergillus niger through soil drenching increased plant dry weight by 38.7 or 53.2%, hectoliter weight by 3.0 or 2.4%, seed yield by 49.1 or 39.5%, and biological yield by 43.4 or 33.6%, respectively, compared to PK0%+C-AN in calcareous soil. The highest P-solubilizing microorganism's population was found at PK0%+C-AN in calcareous soil, while the highest Azotobacter sp. population was observed under high PK levels + C-AN in normal soil. Our study recommends that compost with Aspergillus niger as a bioorganic fertilization treatment can partially substitute PK fertilization and boost quinoa's tolerance to salt calcareous-affected soil.

Coapplication of Effective Microorganisms and Nanomagnesium Boosts the Agronomic, Physio-Biochemical, Osmolytes, and Antioxidants Defenses Against Salt Stress in Ipomoea batatas.

The application of bio- and nanofertilizers are undoubtedly opening new sustainable approaches toward enhancing abiotic stress tolerance in crops. In this study, we evaluated the application of effective microorganisms (EMs) of five groups belonging to photosynthetic bacteria, lactic acid bacteria, yeast, actinobacteria, and fermenting fungi combined with magnesium oxide (MgO) nanoparticles (MgO-NP) on the growth and productivity of sweet potato plants grown in salt-affected soils. In two field experiments carried out in 2020 and 2021, we tested the impacts of EMs using two treatments (with vs. without EMs as soil drench) coupled with three foliar applications of MgO-NP (0, 50, and 100 µg ml-1 of MgO, representing MgO-NP0, MgO-NP50, and MgO-NP100, respectively). In our efforts to investigate the EMs:MgO-NP effects, the performance (growth and yield), nutrient acquisition, and physio-biochemical attributes of sweet potatoes grown in salt-affected soil (7.56 dS m-1) were assessed. Our results revealed that salinity stress significantly reduced the growth parameters, yield traits, photosynthetic pigment content (chlorophylls a and b, and carotenoids), cell membrane stability, relative water content, and nutrient acquisition of sweet potatoes. However, the EMs+ and/or MgO-NP-treated plants showed high tolerance to salt stress, specifically with a relatively superior increase when any of the biostimulants were combined. The application of EMs and/or MgO-NP improved osmotic stress tolerance by increasing the relative water content and membrane integrity. These positive responses owed to increase the osmolytes level (proline, free amino acids, and soluble sugars) and antioxidative compounds (non-enzymatic concentration, enzymatic activities, phenolic acid, and carotenoids). We also noticed that soil salinity significantly increased the Na+ content, whereas EMS+ and/or MgO-NP-treated plants exhibited lower Na+ concentration and increased K+ concentration and K+/Na+ ratio. These improvements contributed to increasing the photosynthetic pigments, growth, and yield under salinity stress. The integrative application of EMs and MgO-NP showed higher efficacy bypassing all single treatments. Our findings indicated the potential of coapplying EMs and MgO-NP for future use in attenuating salt-induced damage beneficially promoting crop performance.

Impact of Folic Acid in Modulating Antioxidant Activity, Osmoprotectants, Anatomical Responses, and Photosynthetic Efficiency of Plectranthus amboinicus Under Salinity Conditions.

Salinity is a major threat to the sustainability of agricultural production systems. Salt stress has unfavorable implications on various plant physio-morphological and biochemical reactions, causing osmotic and ionic stress. Exogenously applied folic acid (FA) may at least provide one mechanism to evade the injurious stress effects of saline irrigation water on Plectranthus amboinicus. In this regard, two pot trials were performed during the 2018-2019 and 2019-2020 seasons in an open greenhouse of an experimental farm (29°17'N; 30°53'E) in Fayoum, Egypt. We tested four levels of saline irrigation water (SW): 34, 68, and 102 mM NaCl, plus tap water as the control = 0), combined with FA at three concentrations (25 and 50 µM, plus spray with distilled water as the control = 0). The growth parameters, biochemistry, physiology, elemental leaf status, essential oil content, and anatomical responses were assessed. Salt markedly reduced photosynthetic productivity [Fv/Fm and performance index (PI)], total chlorophyll [soil plant analysis development (SPAD)], and leaf osmoprotectant compounds, i.e., total soluble sugars (TSS), free amino acids, proline, and total phenolics, thus hampering P. amboinicus growth and essential oil yield. However, the addition of FA as a foliar spray to P. amboinicus irrigated with saline water induced increases in Fv/Fm, SPAD, and PI. These were linked with enriched stem anatomical structures, leaf osmoprotectant compounds, and enhanced leaf enzymatic activity, e.g., superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase, glutathione, ascorbic acid, and antioxidant content. Under salt stress, supplementation of 25 and 50 µM FA increased the growth and production of essential oil by 27.8 and 55.6%, respectively, compared with no applied FA. The highest growth characteristics and elemental leaf contents were obtained when P. amboinicus was irrigated with 0 mM saline water and treated foliarly with 50 µM of FA compared with non-treated plants. Overall, these data showed that foliar spraying with FA reduces the impact of salt stress on P. amboinicus irrigated with saline water.

Soil application of effective microorganisms and nitrogen alleviates salt stress in hot pepper (Capsicum annum L.) plants.

The application of effective microorganisms (EMs) and/or nitrogen (N) have a stimulating effect on plants against abiotic stress conditions. The aim of the present study was to determine the impact of the co-application of EMs and N on growth, physio-biochemical attributes, anatomical structures, nutrients acquisition, capsaicin, protein, and osmoprotectant contents, as well as the antioxidative defense system of hot pepper (Capsicum annum L.) plants. In the field trials, EMs were not applied (EMs-) or applied (EMs+) along with three N rates of 120, 150, and 180 kg unit N ha-1 (designated as N120, N150, and N180, respectively) to hot pepper plants grown in saline soils (9.6 dS m-1). The application of EMs and/or high N levels attenuated the salt-induced damages to hot pepper growth and yield. The application of EMs+ with either N150 or N180 increased the number, average weight and yield of fruits by 14.4 or 17.0%, 20.8 or 20.8% and 28.4 or 27.5%, respectively, compared to hot pepper plants treated with the recommended dose (EMs- × N150). When EMs+ was individually applied or combined with either N150 or N180, increased accumulation of capsaicin were observed by 16.7 or 20.8%, protein by 12.5 or 16.7%, proline by 19.0 or 14.3%, and total soluble sugars by 3.7 or 7.4%, respectively, in comparison with those treated with the integrative EMs- × N150. In addition, the non-enzymatic contents (ascorbate, and glutathione) and enzymatic activities (catalase, superoxide dismutase, and glutathione reductase) of the antioxidant defense systems significantly increased in hot pepper plants treated with EMs+ alone or combined with N150 or N180 under salt stress conditions. Higher accumulation of nutrients (N, P, K+, and Ca2+) along with reduced Na+ acquisition was also evidenced in response to EMs+ or/and high N levels. Most anatomical features of stems and leaves recovered in hot pepper plants grown in saline soils and supplied with EMs+ and N. The application of EMs and N is undoubtedly opening new sustainable approaches toward enhancing abiotic stress tolerance in crops (e.g. hot pepper).

Sequential Antioxidants Foliar Application Can Alleviate Negative Consequences of Salinity Stress in Vicia faba L.

Salinity is one of the most limiting abiotic stresses in agricultural productivity. Exogenously applied antioxidants successfully enabled salt-stressed plants to cope with stress. Two-season field experiments were conducted consecutively in 2016/17 and 2017/18 to study the effects of foliar applications of singular (ascorbate, AsA; proline, Pro; and glutathione, GSH) or sequential (AsA-Pro-GSH and GSH-Pro-AsA) antioxidants on growth, yield, physio-biochemical attributes, and enzymatic and non-enzymatic antioxidative defense system of Vicia faba L. (CV. Sakha-1) plants grown under saline soil conditions (EC = 4.53 dS m-1). Under soil salinity conditions, AsA, Pro, or GSH-Pro-ASA improved growth and productivity, photosynthesis efficiency, stomatal conductance (gs), plant water status, as well as enzymatic and non-enzymatic antioxidants. However, sequential AsA-Pro-GSH foliar application followed by singular GSH significantly exceeded all other treatments (i.e., AsA, Pro, and GSH-Pro-AsA), improving growth characteristics (shoot length, shoot fresh and dry weights, and leaves area), photosynthesis efficiency, stomatal conductance, plant water status, and yield and its components (green pods weight/plant-1, green pods yield/hectare-1, and seed yield/hectare-1), as well as enzymatic (ascorbate peroxidase, catalase, superoxide dismutase, and glutathione reductase) and non-enzymatic (AsA, GSH, Pro, phenolic aglycone, phenolic glycosides) antioxidants compared to control. Overall, our results clearly demonstrate that sequential AsA-Pro-GSH foliar application has a positive effect on salt-stressed Vicia faba plants.

Sequential Application of Antioxidants Rectifies Ion Imbalance and Strengthens Antioxidant Systems in Salt-Stressed Cucumber.

Exogenous antioxidant applications enable salt-stressed plants to successfully cope with different environmental stresses. The objectives of this investigation were to study the effects of sequential treatments of proline (Pro), ascorbic acid (AsA), and/or glutathione (GSH) on 100 mM NaCl-stressed cucumber transplant's physio-biochemical and growth traits as well as systems of antioxidant defense. Under salinity stress, different treatment of AsA, Pro, or/and GSH improved growth characteristics, stomatal conductance (gs), enhanced the activities of glutathione reductase (GR), superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) as well as increased contents of AsA, Pro, and GSH. However, sequential application of antioxidants (GSH-Pro- AsA) significantly exceeded all individual applications, reducing leaf and root Cd2+ and Na+ contents in comparison to the control. In plants grown under NaCl-salt stress, growth characteristics, photosynthetic efficiency, membrane stability index (MSI), relative water content (RWC), contents of root and leaf K+ and Ca2+, and ratios of K+/Na+ and Ca2+/Na+ were notably reduced, while leaf contents of non-enzymatic and enzymatic antioxidants, as well as root and leaf Cd2+ and Na+ concentrations were remarkably increased. However, AsA, Pro, or/and GSH treatments significantly improved all investigated growth characteristics, photosynthetic efficiency, RWC and MSI, as well as AsA, Pro, and GSH, and enzymatic activity, leaf and root K+ and Ca2+ contents and their ratios to Na+, while significantly reduced leaf and root Cd2+ and Na+ contents.

Sequenced ascorbate-proline-glutathione seed treatment elevates cadmium tolerance in cucumber transplants.

During its life cycle, plant has to cope with a number of abiotic stresses including cadmium stress. Cadmium (Cd) is highly toxic to plant and greatly influences its growth and entire metabolism. Antioxidants have to enable plant to beat such stresses. Therefore, effects of ascorbate (AsA), proline (Pro) and glutathione (GSH) applied, as seed soaking solutions, singly or in a sequence on cucumber transplant growth, physio-biochemical attributes and antioxidant defense system activity were investigated under 2 mM Cd stress. Adding Cd to transplants in irrigation water reduced photosynthetic efficiency, and nutrient (K+ and Ca2+) contents, while increased the activity of defense systems (non-enzymatic and enzymatic antioxidants) and Cd2+ contents in roots and leaves. Exogenous AsA, Pro and GSH applied singly or in a sequence improved transplant growth (e.g., shoot length, leaf area, shoot fresh weight and shoot dry weight), photosynthetic efficiency (i.e., SPAD chlorophyll, Fv/Fm and PI), transplant health (i.e., increased leaf MSI and RWC, and decreased root and leaf Cd2+ contents), antioxidant defense systems activity (enzymatic; superoxide dismutase, catalase, glutathione reductase and ascorbate peroxidase, and non-enzymatic; Pro, AsA and GSH antioxidants) and nutrient (K+ and Ca2+) contents. These positive results were obtained under irrigation with or without Cd, AsA. Sequenced AsA-Pro-GSH was the best treatment of which this study recommends to use, followed by GSH treatment, for growing cucumber transplants under Cd stress.

Sequenced application of ascorbate-proline-glutathione improves salt tolerance in maize seedlings.

The role of antioxidants exogenously-applied individually or in sequences in the improvement of salt tolerance in maize seedlings, and their effects on changes in the activities of endogenous enzymatic and non-enzymatic antioxidants, and the concentrations of phytohormones in seedlings grown under 100mM NaCl stress were assessed. The efficiency of maize seedlings to tolerate salt stress in terms of growth was noticed to varying degrees with antioxidants applied singly or in sequences. The healthy growth of salt-stressed seedlings was correlated with the improvements in the activities of enzymatic and non-enzymatic antioxidants, the concentrations of osmoprotectants and phytohormones, and tissue health in terms of relative water content and membrane stability index. Results show that, seed soaking in AsA, GSH and proline applied in sequences (i.e., AsA0.50-Pro0.50-GSH0.50 or GSH0.50-Pro0.50-AsA0.50) was better than their applications individually. In addition, the sequenced application of AsA0.50-Pro0.50-GSH0.50 as integrated treatment was better, generating maize seedlings more tolerant to salinity than those generated from the sequenced application of GSH0.50-Pro0.50-AsA0.50. Therefore, we recommend using the sequenced application of AsA0.50-Pro0.50-GSH0.50 as integrated soaking treatment for maize to grow under salt stress.

Modulation of cadmium toxicity and enhancing cadmium-tolerance in wheat seedlings by exogenous application of polyamines.

Cadmium (Cd) stress causes several negative physiological, biochemical and structural changes due to the oxidative stress caused through the generation of ROS, leading to a reduction in plant growth. To look for an effective method to increase Cd tolerance of wheat seedlings, the effect of presoaking Triticum aestivum L. seeds in spermidine (Spd; 2mM) or spermine (Spm; 2mM) on seedling growth, physiological attributes and antioxidant defence system under 1mM Cd stress were investigated. Spm or Spd alleviated the adverse effects of Cd stress to convergent degrees. Presoaking wheat seeds in either polyamine increased the seedling growth and the activities of antioxidant enzymes compared to the control, but other attributes were slightly affected. Under Cd stress, presoaking seeds in either polyamine significantly increased seedling growth, membrane stability index, relative water content, concentrations of protein, starch, ascorbic acid, total glutathione, Spm and Spd, and the activities of superoxide dismutase and catalase. In contrast, electrolyte leakage, concentrations of proline, total soluble sugars, malondialdehyde, hydrogen peroxide and Cd(2+), and the activities of peroxidase and ascorbate peroxidase were reduced compared to the control. These results are important as the potential of Spd or Spm to alleviate the harmful effects of Cd stress offer an opportunity to increase the resistance of wheat seedlings to growth under Cd stress conditions.

Role of Ulva lactuca extract in alleviation of salinity stress on wheat seedlings.

Seaweeds are potentially excellent sources of highly bioactive materials that could represent useful leads in the alleviation of salinity stress. The effects of presoaking wheat grains in water extract of Ulva lactuca on growth, some enzymatic activities, and protein pattern of salinized plants were investigated in this study. Algal presoaking of grains demonstrated a highly significant enhancement in the percentage of seed germination and growth parameters. The activity of superoxide dismutase (SOD) and catalase (CAT) increased with increasing the algal extract concentration while activity of ascorbate peroxidase (APX) and glutathione reductase (GR) was decreased with increasing concentration of algal extract more than 1% (w/v). The protein pattern of wheat seedling showed 12 newly formed bands as result of algal extract treatments compared with control. The bioactive components in U. lactuca extract such as ascorbic acid, betaine, glutathione, and proline could potentially participate in the alleviation of salinity stress. Therefore, algal presoaking is proved to be an effective technique to improve the growth of wheat seedlings under salt stress conditions.