The Subtype Identity of Testicular Cancer Cells Determines Their Immunostimulatory Activity in a Coculture Model.

Testicular germ cell cancer (TGCC) is subdivided into several subtypes. While seminomatous germ cell tumors (SGCT) are characterized by an intensive infiltration of immune cells which constitute a pro-inflammatory tumor micromilieu (TME), immune cells in non-seminomatous germ cell tumors (NSGCT) are differently composed and less abundant. Previously, we have shown that the seminomatous cell line TCam-2 promotes T cell and monocyte activation in a coculture model, resulting in mutual interactions between both cell types. Here we set out to compare this feature of TCam-2 cells with the non-seminomatous cell line NTERA-2. Peripheral blood T cells or monocytes cocultured with NTERA-2 cells failed to secrete relevant amounts of pro-inflammatory cytokines, and significantly downregulated the expression of genes encoding activation markers and effector molecules. In contrast, immune cells cocultured with TCam-2 cells produced IL-2, IL-6 and TNFα, and strongly upregulated the expression of multiple pro-inflammatory genes. Furthermore, the expression of genes involved in proliferation, stemness and subtype specification remained unaltered in NTERA-2 cells during coculture with T cells or monocytes, indicating the absence of mutual interactions. Collectively, our findings uncover fundamental differences between SGCT and NSGCT in their capability to generate a pro-inflammatory TME, which possibly impacts the clinical features and prognosis of both TGCC subtypes.

Interrater Reliability of Operationalized Psychodynamic Diagnosis 3 (OPD-3).

Objective: The study aims to assess interrater reliability of the revised Operationalized Psychodynamic Diagnosis 3 (OPD-3) system. Three OPD axes were included in the reliability analysis: the Levels of Structural Integration Axis (OPD-LSIA), the Conflict Axis (OPD-CA), and the Interpersonal Relations Axis (OPD-IRA). Methods: A heterogeneous sample of n = 80 patients was diagnostically assessed with SCID-I and SCID-II as well as with an OPD interview. Two raters of a group of five independently rated each patient's OPD interview according to OPD-3. Results: Interrater reliability was good to excellent for OPD-LSIA [ICC1,1 = .874 - .920], and moderate to good forOPD-CA [ICC1,1 = .547 - .764] as well as forOPD-IRA at the aggregated level of Agency and Communion [ICC1,1 = .575 - .793], except for raters' self-perceived tendencies to act towards the patient. Conclusion: The revised OPD-3 can be used as a reliable instrument for a psychodynamic diagnosis of conflicts, levels of structural integration, and dysfunctional relationship patterns.

Can we differentiate between psychoanalytic and psychodynamic psychotherapy? - An empirical investigation of therapists' self-reports.

Therapy differentiation is a crucial component of psychotherapy research. It refers to whether inspected treatments differ from one another. In the history of psychoanalysis, the differentiation between psychodynamic and analytic psychotherapy was often discussed but seldom inspected empirically. In this study, we propose a set of items which should in theory offer the possibility to differentiate between psychodynamic and analytic psychotherapy on session level. We inspect these items using therapists' self-reports concerning N = 295 cases of different psychodynamic and analytic therapies. Results of an exploratory factor analysis and subsequent inspections of the psychodynamic and analytic items in different forms of therapies strengthen the usability of these items for differentiating between psychodynamic and analytic therapy. However, further studies using different perspectives (e. g., observer ratings) are essential. The presented items are a promising step towards the development of an instrument for treatment differentiation which could later be used in treatment comparison studies.

[Reliability and validity of the OPD-conflict-questionnaire in an inpatient treatment sample]. / Reliabilität und Validität des OPD-Konfliktfragebogens bei stationären Psychotherapiepatient*innen.

Reliability and validity of the OPD-conflict-questionnaire in an inpatient treatment sample Objectives: Unconscious conflicts are a major part of psychodynamic diagnostics. Benecke et al. (2018) developed the OPD-conflict-questionnaire (OPD-CQ) to assess unconscious conflicts according to the Operationalized Psychodynamic Diagnostics (OPD) via self-report. We inspected its reliability and validity in a large inpatient sample with a focus on correlations with symptomatic burden, interpersonal problems, and structural level. Methods: N = 2083 patients completed questionnaires at the beginning of their inpatient stay in the Fachklinikum Tiefenbrunn between 2017 and 2020. We calculated internal consistencies of the OPD-CQ scales and (partial-)correlations of the OPD-CQ scales with different instruments. Results: Internal consistencies were only partly satisfying (for eight of 13 scales). We found significant (partial-)correlations of the conflicts with symptom severity and interpersonal problems which were in line with expectations. However, structural level correlated with more conflicts than we expected. Conclusions: Due to the low internal consistencies of some scales, we recommend a revision of the OPD-CQ. Still, the found correlations show the potential of the OPD-CQ as a screening instrument for patients in inpatient treatment.

A psychometric evaluation of the Standardized Assessment of Severity of Personality Disorder (SASPD) in nonclinical and clinical German samples.

The Standardized Assessment of Severity of Personality Disorder (SASPD) is a 9-item self-report screening instrument and was developed to assess personality disorder (PD) severity according to the initial proposal of ICD-11. Our aim was to investigate the psychometric properties of the German version of the SASPD in nonclinical and clinical samples. A total of 1,991 participants (N = 888 from nonclinical and N = 1,103 from clinical samples) provided ratings on the SASPD as well as other measures of psychopathology and personality. We examined the SASPD regarding its factor structure, internal consistency, and construct validity. A unidimensional structure of the SASPD provided inadequate model fit, whereas a 3-factor solution provided good fit in both the nonclinical and clinical samples. Internal consistency of the SASPD total score was acceptable in the clinical and nonclinical samples based on this multifactorial model. In terms of convergent validity, SASPD scores correlated fairly with other measures of PD severity across samples. Discriminant validity with measures of general symptom distress and measures of (normal) personality traits was mixed. In addition, the SASPD scores predicted levels of PD severity above and beyond a measure of symptom distress. The SASPD captures some theoretically expected features of PD severity. However, the multidimensional structure and limited convergent and discriminant validity may hamper future usage of the SASPD as a short screening tool of PD severity according to ICD-11. (PsycInfo Database Record (c) 2020 APA, all rights reserved).

[Difficult situations in psychotherapy and how therapists deal with them]. / Schwierige Situationen in der Psychotherapie und wie Therapeuten mit ihnen umgehen.

Difficult situations in psychotherapy and how therapists deal with them Objectives: In theory and research, it is assumed that therapeutic competences are especially relevant in difficult situations. In the present study, we collected and categorized situations that psychotherapists subjectively evaluated as difficult. Additionally, we inspected therapists' reactions to these situations and considered correlations between situations and reactions. Methods: In an online-survey, 101 therapists described difficult situations and their corresponding reactions. The reports were analyzed by qualitative content analysis (Mayring 2015) and resulted in two category systems for the difficult situations and the reactions. Results: Difficult situations reached from everyday conflicts to extreme situations (e. g. threats). The most frequent difficult situations were in context of therapeutic frame, aspects of disorder, and critics, demands, accusations of patients. The most frequent reactions concerned therapeutic frame, external help and supportive interventions. We found significant correlations between difficult situations due to aspects of disorder and asking for external help. Conclusions: Although categories were sometimes difficult to isolate and few cognitive behavioral therapists participated, the collection of difficult situations can be of help for therapists and their training.

[Levels of structural integration in adolescents and the relationship to later mental disorders - A longitudinal study]. / Psychisches Strukturniveau im Jugendalter und der Zusammenhang mit späterer psychischer Erkrankung ­ eine Langzeitstudie.

Levels of structural integration in adolescents and the relationship to later mental disorders - A longitudinal study Abstract. Objective: Psychological disorders frequently manifest during adolescence. Because of the multifactorial influencing factors, the courses of the diseases are heterogeneous, from relapsing-remitting to chronic. This study investigated whether the level of structural integration of the Operationalized Psychodynamic Diagnostics in Childhood and Adolescence (OPD-CA) correlates with later symptomatic burden. Method: This long-term study assessed the levels of structural integration according to the OPD-CA of 60 adolescents (mean age = 15.6; SD = 0.9). Seven years later, we then measured symptomatic burden (SCID axis I and II) and overall burden (GAF, BSI-GSI) (73.3 % follow-up participation rate). Results: The results showed high correlations between deficient structural integration in adolescence and later symptoms and overall burden in early adulthood. Conclusion: The follow-up examination after a 7-year time period showed significant correlations, which argue for the predictive value of structural integration. This suggests that early specific treatment, e.g., in the form of intensive psychotherapy, be urgently recommended in order to influence this course.

Psychoanalytic Techniques in Different Psychoanalytically Oriented Psychotherapies - An Analysis of Therapists' Self-Reports.

OBJECTIVES: Psychotherapy approaches based on psychoanalytic thinking which also modify standard technique are on the rise. Despite various theoretical considerations, empirical research seldom inspected concepts of practicing therapists concerning technique in different psychoanalytically oriented approaches. METHODS: In an online survey, 124 German psychoanalysts and psychodynamic therapists described their technique on a Psychoanalytic Technique Questionnaire in 295 therapy phases, subjectively assessed as good, of four therapy approaches varying in setting and duration. RESULTS: Factor analysis extracted three therapeutic strategies: a classic, a clarifying, and a supportive technique. Linear mixed model analyses showed that, in therapies with a lower frequency of sessions and face-to-face setting as well as in shorter therapies, classic technique was less often and supportive technique more often used. CONCLUSIONS: Therapists' concepts of different psychoanalytically oriented approaches mostly agree with theoretical assumptions. Future research could inspect patient characteristics associated with specific techniques and the consequences of technique use for treatment success.

[The OPD conflict questionnaire]. / Der OPD-Konfliktfragebogen.

The OPD conflict questionnaire Background: To date, no self-report measure is available to assess modes of coping with unconscious conflicts according to Operationalized Psychodynamic Diagnosis (OPD). The OPD conflict questionnaire (OPD-CQ) developed here thus intends to close this gap. METHODS: To select the items for the OPD-CQ, expert ratings and psychometric criteria based on a sample of 534 persons were combined. RESULTS: The OPD-CQ comprises 66 items with which active and passive modes of coping with six conflicts as well as defended perception of conflict and emotions can be assessed. Some of the scales had a rather low internal consistency. An investigation of the factor structure of the OPD-CQ scales revealed five factors that could be meaningfully interpreted. The OPD-CQ scales showed expected correlations with other clinically relevant instruments. In addition, we showed the incremental validity of the OPD-CQ scales regarding the prediction of psychological distress and life satisfaction above and beyond structural deficits. DISCUSSION: The results provide important clues toward developing and validating the OPDCQ. The first version presented here should be considered preliminary.

A Comparison of Psychoanalytic Therapy and Cognitive Behavioral Therapy for Anxiety (Panic/Agoraphobia) and Personality Disorders (APD Study): Presentation of the RCT Study Design.

OBJECTIVES: Anxiety disorders, most notably panic disorders and agoraphobia, are common mental disorders, and there is a high comorbidity with personality disorders. Randomized controlled trails addressing this highly relevant group of patients are missing. DESIGN: The multicenter Anxiety and Personality Disorders (APD) study investigates 200 patients with panic disorder and/or agoraphobia with comorbid personality disorder in a randomized control-group comparison of psychoanalytic therapy (PT) and cognitive behavioral therapy (CBT), including 100 patients in each group. Each patient will be examined over a period of six years, regardless of the duration of the individual treatment. The main issues that are addressed in this study are the comparison of the efficacy of PT and CBT in this special patient population, the comparison of the sustainability of the effects of PT and CBT, the comparison of the long-term cost-benefit-ratios of PT and CBT as well as the investigation of prescriptive patient characteristics for individualized treatment recommendations (differential indication). DISCUSSION: The APD study compares efficacy, sustainability, and cost-benefit-ratios of CBT and PT for anxiety plus personality disorders in a randomized controlled trail. The study design meets the requirements for an efficacy study for PT, which were recently defined. TRIAL REGISTRATION: Current Controlled Trials ISRCTN12449681.