[Anterolateral stabilization using the modified Lemaire technique for ACL deficiency]. / Anterolaterale Stabilisierung mittels modifizierter Lemaire-Plastik bei insuffizientem vorderem Kreuzband.

OBJECTIVE: Treatment of persistent anterolateral knee instability. INDICATIONS: Subjective/objective (rotational) instability of the knee after anatomic anterior cruciate ligament (ACL) reconstruction. ACL re-rupture including special demands (e.g., high-performance athletes, hyperlaxity) RELATIVE CONTRAINDICATIONS: Osteoarthritis, additional instability of the knee, which should be treated independently; non-anatomic ACL reconstruction with persisting instability should be treated first with anatomic ACL reconstruction. ABSOLUTE CONTRAINDICATIONS: General contraindications for surgery (e. g. septic arthritis), acute irritation of the affected knee. SURGICAL TECHNIQUE: Supine position. Incision along the proximal lateral femoral epicondyle. Marking of the needed width and length of the iliotibial band (ITB) graft. Passing the ITB graft underneath the lateral collateral ligament. Find and mark the isometric point for fixation next to the lateral femoral epicondyle. Fixation of the ITB graft. Layered wound closure. POSTOPERATIVE MANAGEMENT: Knee brace for at least 6 weeks. Range of motion (RoM): from postoperative day 1: flexion-extension 90-0-0°; first 2 weeks after surgery: partial weight bearing (20 kg). RESULTS: An anterolateral extra-articular reconstruction may reduce a persistent anterolateral rotatory instability as well as the re-rupture rate following ACL reconstruction with good patient-reported short-term outcomes. Based on current (biomechanical) data, anterolateral tenodesis seems to be superior to a reconstruction of the anterolateral ligament. If a tenodesis is performed, the graft should be fixed in an isometric position, with neutral rotation of the knee and low graft tension to avoid extraphysiologic load within the lateral compartment. Indications for such a procedure may include a high-grade pivot shift or revision ACL reconstruction as well as a persistent anterolateral rotatory instability following anatomic ACL reconstruction.

The impact of different peripheral suture techniques on the biomechanical stability in flexor tendon repair.

PURPOSE: Flexor tendon repair consists of circumferential peripheral sutures in combination with core sutures to avoid fraying and reduces the exposure of suture material on tendon surface. The peripheral suture adds up to a tenfold increase of the biomechanical stability compared to the core suture alone. The purpose of our study was to determine the most favourable peripheral repair technique for tendon repair. METHODS: Seventy-two porcine flexor tendons underwent standardized tenotomy and repair using one of the following six methods (n = 12): simple-running (SR), simple-locking (SL), Halsted-mattress (HM), lin-locking (LL), Lembert-mattress (LM), and Silfverskiöld cross-stich (SCS) suture technique. The SL- suture was placed 2 mm; the HM, LM, SC, and LL suture were placed 5 mm from the tendon gap. The SR suture was placed 1, 2, and 3 mm from tendon ends; no additional core suture was applied. For cyclic testing (1000 cycles), elongation was calculated; for load to failure construct stiffness, yield load and maximum load were determined. RESULTS: The mean cyclic elongation for all tested suture techniques was less than 2 mm; there was no significant difference between the groups regarding elongation as well as yield load. The HM, LM, SCS, and LL suture techniques presented significantly higher maximum loads compared to the SR- and SL-sutures. The 3 mm SR showed significantly higher maximum loads compared to the 2 and 1 mm SR. CONCLUSIONS: Beside the distance from tendon gap, the type of linkage of the suture material across and beneath the epitendineum is important for biomechanical stability. Simple-running suture is easy to use, even with a slight increase of the distance from tendon gap significantly increases biomechanical strength. For future repairs of flexor tendon injuries, 3 mm stitch length is highly recommended for simple peripheral suture, while the Halsted-mattress suture unites the most important qualities: biomechanically strong, most part of suture material placed epitendinous, and not too complicated to perform.

Converting round tendons to flat tendon constructs: Does the preparation process have an influence on the structural properties?

PURPOSE: The structural properties of hamstring tendon grafts were evaluated in a porcine model, after processing it to a flat shape, to better replace or augment anatomic flat structures (e.g. ACL, MPFL or MCL). METHODS: In this biomechanical study, porcine flexor tendons were used which have a comparable shape to semitendinosus and gracilis tendons. One part of the tendon was prepared to a flat tendon construct by splitting the tendon longitudinally with a knife to half of the diameter of the tendon. The semi-split tendon was scratched out to a flat shape. The other matched part was tested in its original round shape. The tendons (n = 40) have been fixed in a uniaxial testing machine (Zwick/Roell) by cryo-clamps after preparing the fixed ends by 2-0 polyester sutures (2-0 Ethibond® EXCEL, Ethicon, Somerville, NJ). In every specimen, there was a free 60-mm tendon part between both clamps. The tendons have been loaded to failure to evaluate typical biomechanical parameters such as stiffness, yield load and maximum load. RESULTS: No statistically significant differences (n.s.) regarding stiffness, yield load and maximum load between natively round and processed flat tendons could be detected. CONCLUSION: A prepared flat-shaped tendon does not show any different structural properties compared with an original round tendon. Therefore, a flat tendon seems to be a biomechanical stable graft option for anatomic reconstruction or augmentation of injured natively flat-shaped structures such as MCL, MPFL or ACL.

Evaluation of biomechanical properties: are porcine flexor tendons and bovine extensor tendons eligible surrogates for human tendons in in vitro studies?

INTRODUCTION: Porcine flexor tendons, bovine extensor tendons, and human (semitendinosus) tendons are frequently used as substitutes for human ACL grafts in biomechanical in vitro studies. This study compares the biomechanical properties and structural differences of these tendons. MATERIALS AND METHODS: In this biomechanical study, fresh-frozen porcine flexor tendons, bovine extensor tendons, and human semitendinosus tendons were used (n = 36). The tendons were mounted in a uniaxial testing machine (Zwick/Roell) with cryo-clamps, leaving a 60 mm tendon part free between the two clamps. Specimens have been loaded to failure to evaluate the biomechanical parameters stiffness, yield load, and maximum load. A Total Collagen Assay Kit was used to detect differences in the total collagen type I concentration (n = 30). A one-way ANOVA was performed to detect differences in the means. The significance level was set at p < 0.05. RESULTS: There were no significant differences in the stiffness between the groups (bovine 194 ± 43 N/mm, porcine 211 ± 63 N/mm, and human cadaveric 208 ± 58 N/mm). The yield and maximum loads were high (>1000 N) in all groups, but they were significantly increased in both animal specimens (means of 1681-1795 N) compared with human cadaveric specimen (means of 1289-1406 N; p < 0.01). No difference in the collagen type I concentration was detected (N.S.). CONCLUSION: Porcine flexor and bovine extensor tendons are eligible substitutes with similar stiffness and high failure loads compared with human cadaveric semitendinosus tendons in in vitro studies.

[Medial patellofemoral ligament reconstruction using quadriceps tendon]. / Rekonstruktion eines medialen patellofemoralen Ligaments mit Quadrizepssehnenstreifen.

OBJECTIVE: Stabilization of the patella by medial patellofemoral ligament (MPFL) reconstruction. INDICATIONS: Recurrent lateral patella instability with chronic weakening of the MPFL. CONTRAINDICATIONS: Femoropatellar cartilage defects ICRS grade 3° or higher. Tuberositas Tibiae Trochlear Groove Index (TTTG) >20 mm. Lateral hypercompression of the patella without instability. SURGICAL TECHNIQUE: A 3 cm transverse skin incision at the superomedial edge of the patella in 90° of flexion. Longitudinal incision of the prepatellar bursa and exposure of the quadriceps tendon. Preparation of a flat tendon strip with a length of 8 cm, a width of 10 mm, and a thickness of 3 mm, leaving the attachment at the patella intact. Flipping of the tendon strip and passing of the graft through a tunnel underneath the prepatellar tissue at the medial edge of the patella. Passing of the graft in layer two of the medial joint capsule just below the fascia (layer 1) and the vastus medialis. Fixation of the graft in a bone tunnel, drilled in the femoral insertion site of the native MPFL using a biodegradable interference screw. POSTOPERATIVE MANAGEMENT: Patella centralizing brace for 4 weeks with range of motion (ROM) 0/0/90°, 20 kg of partial weight bearing for 3 weeks. Full weight-bearing according to pain starting from week 4 postoperatively. ROM up to 90° of flexion directly postoperatively. Free ROM starting from week 6 postoperatively. Stationary cycling 6 weeks postoperatively. Swimming and running after 10 months. Return to pivoting sports after 4-5 months. RESULTS: A total of 17 patients (7 men and 10 women; average age 21.5 years ±3.9 years, average BMI 22.6 ±3.9) were treated using this technique between March 2011 and November 2012. Only patients with at least one recurrent patella dislocation following conservative treatment were included. Patient satisfaction 12 months postoperatively was very high. Overall, 94.1 % would undergo the procedure again and 94.1 % were very satisfied with the cosmetic result. Significant improvement in Lysholm score 6, 12, and 24 months postoperatively (p < 0.05). Free ROM in 94.1 % of cases 12 months postoperatively. The Kujala score was reported to be 89 (±7.1) 12 months following surgery. No redislocation occurred during the 24 months following surgery.

[Dynamic intraligamentary stabilization of the anterior cruciate ligament. Operative technique and short-term clinical results]. / Dynamische intraligamentäre Stabilisierung des vorderen Kreuzbandes. Operationstechnik und klinische Kurzzeitergebnisse.

BACKGROUND: Reconstruction of tears in the anterior cruciate ligament with a tendon graft is the current gold standard. OBJECTIVES: Dynamic intraligamentary stabilization is a new technique for preservation of the anterior cruciate ligament. METHODS: This article describes the indications, operative technique, rehabilitation and preliminary results after dynamic intraligamentary stabilization for acute anterior cruciate ligament ruptures. RESULTS: A total of 24 women and 31 men with an acute anterior cruciate ligament tear were included in a prospective clinical trial. Of the patients 26 had already been followed-up for 12 months and satisfying values for the Lysholm, Tegner and International Knee Documentation Committee (IKDC) outcome scores were achieved. High subjective patient satisfaction was also achieved. The Lachman test showed a mean anterior translation difference to the healthy side of 1.7 mm. CONCLUSION: Dynamic intraligamentary stabilization in combination with microfracturing of the notch can provide biomechanical and biological conditions for self-healing of the anterior cruciate ligament. Further clinical and biomechanical research is needed to identify appropriate patients and rupture types suitable for this new technique.

[Arthroscopic treatment of tibial plateau fractures]. / Arthroskopisch gestützte Versorgung der Tibiakopffraktur.

OBJECTIVE: The aim of arthroscopically assisted treatment of tibial plateau fractures is to achieve minimally invasive reduction and internal fixation of the joint fracture of the tibial plateau. Using the arthroscopic procedure, both the approach morbidity and the control of the articular reduction can be optimized. INDICATIONS: Displaced tibia plateau fractures of AO type A1 and B1/2/3 or Tscherne P2. CONTRAINDICATIONS: Strongly displaced tibial plateau fractures, which require an open surgical approach and stabilization with plate fixation (e.g., AO type C fractures or Moore type 5 fractures); 2nd and 3rd degree open fractures. Danger of compartment syndrome. SURGICAL TECHNIQUE: Planning of the surgical approach and confirmation of the indication by CT imaging. Diagnostic arthroscopy of the knee joint with treatment of associated injuries and confirming the indications for arthroscopically assisted reduction. Under arthroscopic control, insertion of an ACL tibial aiming device. In the central portion of the dislocated fracture fragment, a 2.4 mm K-wire is placed with the help of the aiming device. Opening of the outer cortex using a cannulated drill (9-11 mm diameter), introduction of a cannulated plunger below the fracture resulting in reduction of the fracture and compression of the cancellous bone below the fracture. Simultaneously the reduction is controlled by arthroscopy. Finally, the fracture is fixed using minimally invasive screw fixation (3.5-7.3 mm cancellous screws with washers) or by plate osteosynthesis (e.g., support plate). The metaphyseal defect can optionally be filled with bone substitute material. POSTOPERATIVE MANAGEMENT: Rehabilitation is dependent on the extent of the fracture. In arthroscopically treated fractures, partial weight bearing of 20 kg over a period of 6-12 weeks is usually necessary.

[Stage-adapted treatment of infection after reconstruction of the anterior cruciate ligament]. / Stadiengerechte Therapie bei Infekten nach Ersatzplastik des vorderen Kreuzbandes.

OBJECTIVE: Debridement and irrigation of the knee joint and the donor site to reduce the amount of bacteria and to remove bacterial enzymes and inflammatory cytokines in cases with infection after replacement of the anterior cruciate ligament (ACL). INDICATIONS: Proven infection, suspected infection. CONTRAINDICATIONS: High anesthesiological risk. SURGICAL TECHNIQUE: The therapy of infections after ACL reconstruction depends on the different stages as described by Gächter. In stage I and II, arthroscopic debridement and irrigation (about 10 l) of all compartments is performed. Several biopsies for microbiological analysis are obtained during surgery. Broad-spectrum antibiotic therapy started immediately after surgery. Once the resistance of the bacteria is known, antibiotic therapy may be changed according to the results. When pain, swelling, elevated CRP, or white blood cells persists, arthroscopic irrigation is repeated. In stage III, a complete arthroscopic synovectomy is performed. When the graft is intact, it is left in situ. Resection is performed in case of graft insufficiency, tunnel malplacement, or persistent infection. When the bone tunnels are affected, they should be debrided. In stage IV with osteolysis, an arthrotomy with open debridement may be required. The surgical therapy can be supported with the application of local antibiotics. REHABILITATION: Redon drainage is left in situ until the next irrigation is performed, control of wound and laboratory results, passive exercises, physiotherapy, systemic antibiotic therapy. RESULTS: Between 2008 and 2013, we treated 13 patients with an infection after replacement of the ACL. In all cases, complete healing was achieved. In 4 cases, the graft was resected. In 2 patients, arthrofibrosis developed and arthrolysis was required.

Root tear of the meniscus: biomechanical evaluation of an arthroscopic refixation technique.

BACKGROUND: Aim of the current study was to evaluate the structural properties of an arthroscopic refixation technique for meniscal root tears. HYPOTHESIS: Arthroscopic two suture root tear refixation technique using a transtibial approach restores the structural properties of the intact meniscus attachment more closely than a one suture technique. METHODS: Lateral root tear refixation was performed in a porcine knee model. Using a material testing machine, structural properties were determined after a cyclic loading protocol comparing transtibial tunnel reconstruction using one or a double suture technique. Intact posterior horn served as control group. RESULTS: Elongation after cyclic testing was significantly lower for intact and two suture technique when compared to single suture technique. Stiffness was significantly higher for intact constructs with a mean of 53.7 (±6.5) N/m and two suture technique with 44.8 (±9.9) N/m when compared to one suture technique with a mean of 37.1 (±5.4) N/m. In elongation and stiffness, no differences were found between intact and two suture technique. Ultimate failure loads were 325.6 (±77) N for the intact, 273.6 (±45.6) N for two suture technique and 149.8 (±24.3) N for the one suture technique. Both reconstruction techniques showed significantly lower ultimate failure load when compared to the intact control. CONCLUSION: Structural properties of root tear fixation using a transtibial single suture technique showed significantly higher elongation and lower stiffness and failure load after cyclic loading compared to the intact, whereas a two suture technique showed no difference in elongation and stiffness; however, lower failure load.

[Ligament ruptures of the lower extremity in the elderly]. / Bandverletzungen der unteren Extremität beim älteren Menschen.

There is an increasing incidence of ligament ruptures of the lower extremities in older patients. This higher incidence is caused by the typical current demographic changes in the population and the higher level of activity and athletic motivation of the older people in our society. In this review we address the most important ligament ruptures of the lower extremity in the old patient. Quadriceps tendon and Achilles tendon ruptures are mostly the result of degenerative and abrasion changes. The ACL rupture on the other hand occurs spontaneously after adequate trauma and without former degenerative changes especially in highly active patients. For a differentiated treatment of the older patient with tendon ruptures, secondary diseases, an increased risk and complication profile and a potentially decreased compliance during rehabilitation must be taken into consideration before indicating operative or conservative therapy. There are no strict age-related limitations for indication of an operative treatment of tendon ruptures in the older patient. In this patient group a differentiated treatment decision is recommended.

Structural properties of a new device for graft fixation in cruciate ligament reconstruction: the shim technique.

INTRODUCTION: In ACL reconstruction using a soft tissue graft, aperture fixation with interference screws (IFS) can lacerate and rotate the graft and cause primary tunnel widening. To overcome these downsides, a new wedge-shaped implant (MegaShim, Karl Storz, Germany) was developed. We hypothesized that aperture fixation of hamstring ACL reconstruction using the MegaShim technique shows comparable structural properties when compared to interference screw fixation. METHODS: In a porcine knee model, ACL reconstructions with a tunnel diameter of 6, 7, 8 and 9 mm were performed and human hamstring grafts were fixed using the MegaShim technique (group I). In group 2, grafts were fixed in a hybrid fixation using a MegaShim and cortical flip button (FlippTack, Karl Storz, Germany). Interference screw graft fixation served as a control group (group III). Maximum load, yield load and stiffness were recorded using material testing machine. Grafts were cyclically preconditioned between 0 and 20 N for 10 cycles before the graft-bone complex was loaded to failure. Statistical evaluation was performed using SPSS Version 11.0. RESULTS: Mean maximum load to failure for the hybrid fixation was significantly higher than after interference screw or MegaShim fixation. The difference between MegaShim and interference screw fixation showed no significant difference for 6 and 7 mm sizes. An 8 and 9 mm MegaShim fixation resulted in significantly lower ultimate failure load compared to interference screw fixation. Stiffness of grafts fixed using 6 mm MegaShim was significantly lower than grafts fixed with hybrid or interference screw fixation, whereas no significant differences were found in the 7, 8, and 9 mm fixations. CONCLUSION: Aperture fixation using the MegaShim technique provides comparable structural properties compared to interference screw fixation. Hybrid fixation using MegaShim and cortical flip button results in significantly higher ultimate failure loads than both aperture fixation approaches. Smaller grafts (6 mm) showed significantly lower ultimate failure load and stiffness than interference screw fixation. The "MegaShim technique" is an alternative to interference screw fixation concerning initial fixation strength.

[3D navigated osteosynthesis of calcaneal fractures. Open and minimally invasive techniques]. / 3D-navigierte Fersenbeinosteosynthese: Offene und minimal-invasive Technik.

BACKGROUND: It is hypothesized that misplacement of sustentacular screws during osteosynthesis of intraarticular calcaneal fractures can be reduced with the help of navigation. A method for three-dimensional (3D) navigated placement of sustentacular screws for treating intraarticular calcaneal fractures is presented and evaluated. MATERIAL AND METHODS: 11 consecutive patients with 15 intraarticular calcaneal fractures were treated using 3D navigation. In 12 cases osteosynthesis was done through an extended lateral approach; in three cases, it was achieved through a minimally invasive percutaneous approach. For verification and documentation of the placed screws, a second 3D scan was performed. RESULTS: A total of 20 screws were placed using 3D navigation. None of the navigated screws was misplaced. Extra operating time due to navigation averaged 11.9 minutes (+/-2.2 min). CONCLUSION: Through a combination of intraoperative 3D imaging and navigation, placement of sustentacular screws is possible and can yield precise and reliable results. Especially in minimally invasive treatment, a high quality of osteosynthesis can be achieved.

Mechanical response of cervical vertebral endplates to axial loading.

OBJECTIVE: After anterior cervical discectomy the implantation of a spacer is common practice. The majority of these spacers are trapezoid titanium cages. During the development of a height-adjustable cervical implant we needed to establish the testing limits for this device. A known phenomenon is subsidence of the cage into the vertebral endplates, which leads to a decrease in height and/or angulation of the cervical spinal segment. In contrast to the thoracic and lumbar spines, there are only limited data concerning the load-bearing ability of cervical endplates. The aim of our investigation was to obtain these data. METHODS: Bone density of 16 cervical vertebrae was estimated by quantitative computed tomography. After embedding of the vertebrae into PMMA, each endplate was slowly compressed until failure using a metal indenter resembling the form of a newly developed cervical implant. A fixed protocol with increasing loading cycles was followed. Endpoint was breakage of the endplate as established by failure to resist the increasing loading forces produced by the testing machine. RESULTS: The mean bone density of the 16 cervical vertebrae was 204 with a standard deviation of 52 mg Ca-HA/mL (range 130-281). The endplates failed with a mean loading of 1084 N +/- 314 (range 340-1550 N). The maximum load correlates with the bone density (R2 = 0.7347). With the 97.79 mm2 load bearing surface of the cage we calculate a mean cervical endplate break strength of 10.47 MPa and a 95 % confidence interval of 12.66-9.51 MPa. An initial settling produced by resting of the anchoring teeth in the cervical endplates was observed in 8 vertebrae at a load of 113 N (range 50-250 N). CONCLUSIONS: In contrast to the thoracic and lumbar spines, cervical endplates show a lower resistance against axial forces. The data are important to understand postoperative cage subsidence and to establish testing limits for the development of new implant designs.

Temporal expression of the Bacillus subtilis secA gene, encoding a central component of the preprotein translocase.

In Bacillus subtilis, the secretion of extracellular proteins strongly increases upon transition from exponential growth to the stationary growth phase. It is not known whether the amounts of some or all components of the protein translocation apparatus are concomitantly increased in relation to the increased export activity. In this study, we analyzed the transcriptional organization and temporal expression of the secA gene, encoding a central component of the B. subtilis preprotein translocase. We found that secA and the downstream gene (prfB) constitute an operon that is transcribed from a vegetative (sigmaA-dependent) promoter located upstream of secA. Furthermore, using different independent methods, we found that secA expression occurred mainly in the exponential growth phase, reaching a maximal value almost precisely at the transition from exponential growth to the stationary growth phase. Following to this maximum, the de novo transcription of secA sharply decreased to a low basal level. Since at the time of maximal secA transcription the secretion activity of B. subtilis strongly increases, our results clearly demonstrate that the expression of at least one of the central components of the B. subtilis protein export apparatus is adapted to the increased demand for protein secretion. Possible mechanistic consequences are discussed.

Use of the pre-pro part of Staphylococcus hyicus lipase as a carrier for secretion of Escherichia coli outer membrane protein A (OmpA) prevents proteolytic degradation of OmpA by cell-associated protease(s) in two different gram-positive bacteria.

Heterologous protein secretion was studied in the gram-positive bacteria Bacillus subtilis and Staphylococcus carnosus by using the Escherichia coli outer membrane protein OmpA as a model protein. The OmpA protein was found to be translocated across the plasma membrane of both microorganisms. However, the majority of the translocated OmpA was similarly degraded in B. subtilis and S. carnosus despite the fact that the latter organism does not secrete soluble exoproteases into the culture medium. The finding that purified OmpA, which was added externally to the culture medium of growing S. carnosus cells, remained intact indicates that newly synthesized and exported OmpA is degraded by one or more cell-associated proteases rather than by a soluble exoprotease. Fusion of the mature part of OmpA to the pre-pro part of a lipase from Staphylococcus hyicus allowed the efficient release of the corresponding propeptide-OmpA hybrid protein into the supernatant and completely prevented the cell-associated proteolytic degradation of the mature OmpA, most likely reflecting an important function of the propeptide during secretion of its natural mature lipase moiety. The relevance of our findings for the biotechnological use of gram-positive bacteria as host organisms for the secretory production of heterologous proteins is discussed.

Cloning and sequencing of the dnaK operon of Bacillus stearothermophilus.

Here, we report the cloning of a 5.8-kb PstI fragment of chromosomal DNA from Bacillus stearothermophilus (Bt) carrying the three genes, grpE, dnaK and dnaJ. This fragment contains, in addition, the 3'-end of an open reading frame which has been shown to be part of the dnaK operon in three bacterial species. The dnaJ gene could complement an Escherichia coli dnaJts mutant for growth at high temperature. Sequencing and hybridization data strongly suggest that the Bt chromosome contains an analog of dnaJ.