RESUMO
Forkhead box protein 3 (FoxP3)(+) regulatory T cells (Tregs ) are important not only in regulating the development of autoimmune conditions, but also in chronic infectious diseases. Given their cardinal function in suppressing immune activation, research has focused upon whether they play a detrimental role in chronic infections, particularly HIV. While the role of Tregs in HIV has been investigated intensively, it remains an unresolved topic. However, it is generally accepted that Tregs are susceptible to HIV infection and are preferentially preserved over conventional CD4(+) T cells. It is unknown whether the peripheral-induced or the thymic-derived Tregs are more susceptible to HIV cytotoxicity. It has been recognized that Tregs can be segregated into two subsets based on Helios expression, with the vast majority being Helios(+) . This study examines the impact of HIV infection on total Tregs and their Helios subsets in a perinatal-acquired HIV-infected paediatric population. The finding indicates a selective expansion or survival of Tregs in association with CD4 depletion and increased viraemia. The Helios(+) and Helios(-) subsets within Tregs appear to be equally affected. However, the Helios(+) Tregs seem to be more preserved in patients with low CD4(+) ≤ 25% and detectable plasma HIV RNA >20 copies/ml. In this group, the frequencies of Tregs are increased, but their numbers appear insufficient to restrain immune activation. In conclusion, our findings suggest that both Helios subsets of Tregs are susceptible to HIV infection and are preferentially preserved compared to conventional CD4(+) T cells.
Assuntos
Fatores de Transcrição Forkhead/imunologia , Regulação da Expressão Gênica/imunologia , Infecções por HIV/imunologia , HIV-1/imunologia , Fator de Transcrição Ikaros/imunologia , Linfócitos T Reguladores/imunologia , Adolescente , Adulto , Criança , Pré-Escolar , Doença Crônica , Feminino , Fatores de Transcrição Forkhead/biossíntese , Infecções por HIV/sangue , Infecções por HIV/congênito , Infecções por HIV/patologia , HIV-1/metabolismo , Humanos , Fator de Transcrição Ikaros/biossíntese , Lactente , Masculino , RNA Viral/sangue , RNA Viral/imunologia , Linfócitos T Reguladores/metabolismo , Linfócitos T Reguladores/patologia , Timo/imunologia , Timo/metabolismo , Timo/patologiaRESUMO
We report the development of Campylobacter jejuni enteritis in a patient with preexisting humoral and cellular immune recognition of C. jejuni antigens. This is one of few studies in which the immunologic status of a person with regard to C. jejuni before and after C. jejuni infection is directly compared, and it is the only study of which we are aware that includes measurements of cellular immunity. The findings may be important to Campylobacter vaccine development efforts.
Assuntos
Infecções por Campylobacter/imunologia , Campylobacter jejuni , Enterite/imunologia , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias , Infecções por Campylobacter/etiologia , Campylobacter jejuni/imunologia , Humanos , Imunidade Celular , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Masculino , Pessoa de Meia-IdadeRESUMO
A preterm breast-fed infant had three episodes of type Ia/c group B streptococcus septicemia. After the second episode rifampin was given to the infant, but further Ia/c exposure to maternal breast milk ensued. We propose rifampin treatment for both the mother and infant in cases of recurrent group B streptococcus disease.
Assuntos
Hansenostáticos/uso terapêutico , Leite Humano/microbiologia , Rifampina/uso terapêutico , Infecções Estreptocócicas/tratamento farmacológico , Streptococcus agalactiae/isolamento & purificação , Aleitamento Materno , Eletroforese em Gel de Campo Pulsado , Feminino , Humanos , Recém-Nascido , RecidivaAssuntos
Infecções Oportunistas Relacionadas com a AIDS/complicações , Pneumonia por Pneumocystis/complicações , Infecções Oportunistas Relacionadas com a AIDS/imunologia , Adulto , Contagem de Linfócito CD4 , Feminino , Infecções por HIV/complicações , Infecções por HIV/diagnóstico , Infecções por HIV/transmissão , Humanos , Imunocompetência , Lactente , Recém-Nascido , Troca Materno-Fetal , Pneumonia por Pneumocystis/imunologia , Gravidez , Complicações Infecciosas na GravidezRESUMO
Shigella sonnei infection resulting from oral administration of 500 colony-forming units was followed in 11 volunteers with the objective of studying the immune response and pathogenesis. Characterization of infection included recording of signs and symptoms, excretion of S. sonnei in stool, measurement of humoral tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interferon-gamma (IFN-gamma), C-reactive protein, IL-2 receptor, soluble CD8, antibody-antigen complexes, and endotoxin. Measurements were also made of the immune response including lymphocytes secreting antibody to S. sonnei O antigen and serum antibody to this antigen. Six of the volunteers developed typical shigellosis with excretion of bacteria in stool and systemic signs and symptoms, three excreted bacteria but did not show illness, and two showed no evidence of infection or illness. Shigellosis was characterized by excretion in stool of S. sonnei beginning on average 1.3 days after ingestion. Excretion of S. sonnei (mean of time of the first positive cultures) was followed in sequence by the onset of increases in TNF-alpha (10 hr), liquid stools (14 hr), fever and dysentery (18 hr), IFN-gamma (22 hr), and C-reactive protein (34 hr). A S. sonnei-specific immune response was demonstrated somewhat later, between days 4 and 7 postinfection by antibody-secreting cells, and between days 7 and 14 postinfection by humoral antibody. Shigellosis was not associated with increased humoral IL-1 beta, endotoxin, or antigen-antibody complexes.
Assuntos
Proteínas de Fase Aguda/análise , Anticorpos Antibacterianos/análise , Citocinas/análise , Disenteria Bacilar/imunologia , Shigella sonnei/imunologia , Adolescente , Adulto , Células Produtoras de Anticorpos/imunologia , Antígenos de Bactérias/imunologia , Proteína C-Reativa/análise , Disenteria Bacilar/etiologia , Fezes/microbiologia , Feminino , Humanos , Interferon gama/análise , Masculino , Shigella sonnei/isolamento & purificação , Fator de Necrose Tumoral alfa/análiseRESUMO
Production of leukocyte migration inhibition factor was measured in vitro with purified protein derivative and phytohemagglutinin in 14 marasmic infants 6 to 18 months of age. Twenty-seven well-nourished infants served as controls. All the children had received BCG vaccine in the neonatal period. Tuberculin reaction was positive in four of 14 of the marasmic infants and 13 of 27 of the controls. When leukocyte migration inhibition factor was induced with purified protein derivative, the four tuberculin positive malnourished subjects had a mean migration inhibition index of 55.7% which was significantly higher than the mean migration inhibition index of 38.2% in the tuberculin positive controls. In the tuberculin negative subjects the mean migration inhibition index was 24.7 and 16.6% in the marasmic and control groups, respectively. The difference was not statistically significant. Phytohemagglutinin-induced migration inhibition was comparable in malnourished and control infants. There was no correlation between leukocyte migration inhibition factor production and biochemical indices of iron nutrition, erythrocyte or serum folate, vitamin A, carotene, or serum zinc levels in either group. It is suggested the capacity of lymphocytes to produce leukocyte migration inhibition factor is unchanged in marasmus.