Exploring Reversible Redox Behavior in the 6H-BaFeO3-δ (0 < δ < 0.4) System: Impact of Fe3+/Fe4+ Ratio on CO Oxidation.

This work is devoted to evaluating the relationship between the oxygen content and catalytic activity in the CO oxidation process of the 6H-type BaFeO3-δ system. Strong evidence is provided about the improvement of catalytic performance with increasing Fe average oxidation state, thus suggesting the involvement of lattice oxygen in the catalytic process. The compositional and structural changes taking place in both the anionic and cationic sublattices of the catalysts during redox cycles have been determined by temperature-resolved neutron diffraction. The obtained results evidence a structural transition from hexagonal (P63/mmc) to orthorhombic (Cmcm) symmetry. This transition is linked to octahedra distortion when the Fe3+ concentration exceeds 40% (δ values higher than 0.2). The topotactical character of the redox process is maintained in the δ range 0 < δ < 0.4. This suggests that the cationic framework is only subjected to slight structural modifications during the oxygen exchange process occurring during the catalytic cycle.

Le fort I osteotomy with or without concomitant removal of upper third molars: A prospective cohort study of intraoperative findings, related complications, and level of pterygomaxillary separation after down-fracture.

The main objective of our study was to assess the clinical implications of concomitant intraoperative removal of maxillary third molars (M3M) with the Le Fort I osteotomy 'twist' technique (LF1twist). A prospective comparative study was carried out regarding the necessity of bone trimming around the pedicle, intra- and postoperative complications, surgery time, and the level of pterygomaxillary junction (PMJ) separation following LF1twist with concomitant intraoperative removal of M3M (test group) versus LF1twist alone (control group). Outcome parameters were assessed from clinical and radiographic measurements. In total, 100 surgery sites were included (50 in each group). The mean surgery time was 53.4 ± 7.8 min, with nonsignificant differences between groups. The frequency of intraoperative complications was negligible, with no significant differences between groups - though all bleeding events (n = 4) occurred when M3M was not removed concomitantly. No postoperative complications were recorded. Percentages of disarticulations occurring at the PMJ were similar in both groups. In conclusion, the results demonstrate that removal of M3M in conjunction with LF1twist does not imply additional surgery time, or differences regarding the level of PMJ separation or perioperative complications. Furthermore, the concomitant procedure reduces the amount of maxillary and palatal bone in the disjunction area, which facilitates down-fracture and field clearing for maxillary repositioning.

Does orthognathic surgery have an incidentally beneficial effect on mild or asymptomatic sleep apnoea?

A prospective study was performed to assess the effect of orthognathic surgery on mild obstructive sleep apnoea (OSA) in patients with an underlying dentofacial deformity treated for occlusal and/or aesthetic reasons. As the main outcome variables, changes in upper airway volume and apnoea-hypopnoea index (AHI) were evaluated at 1 and 12 months of follow-up, in patients undergoing orthognathic surgery with widening movements of the maxillomandibular complex. Descriptive, bivariate, and correlation analyses were performed; significance was set at P < 0.05. Eighteen patients diagnosed with mild OSA were enroled (mean age 39.8 ± 10.0 years). An overall upper airway volume widening of 46.7% after orthognathic surgery was observed at 12 months of follow-up. The AHI decreased significantly from a median 7.7 events/hour preoperatively to 5.0 events/h at 12 months postoperative (P = 0.045), and the Epworth Sleepiness Scale score decreased from a median 9.5 preoperatively to 7 at 12 months postoperative (P = 0.009). A cure rate of 50% was obtained at 12 months of follow-up (P = 0.009). Despite the limited sample size, this study provides evidence that in patients with an underlying retrusive dentofacial deformity and mild OSA, a slight decrease in AHI is obtained after orthognathic surgery due to upper airway enlargement, which could be added as a beneficial effect of orthognathic surgery.

'Pillow technique' to improve lip support in the context of zygoma implant rehabilitation.

This study proposes a simple, off-the-shelf ancillary method for application in the dental rehabilitation of severe maxillary atrophy with zygoma implants, allowing simultaneous improvement of lip support in cases with a moderate lack of premaxillary projection. Three consecutive patients with an atrophic maxilla were evaluated retrospectively. All were treated with a fixed rehabilitation over four zygomatic implants and the pillow technique. The study variables included radiological assessment of the premaxilla volume, upper lip and perinasal soft tissue changes, clinical complications, and subjective evaluation of functional and aesthetic patient satisfaction based on a visual analogue scale. All of the zygomatic implants showed osseointegration. The survival rate was 100%. The immediate postoperative course was uneventful, and no surgical complications were noted at the follow-up visits. Radiological assessment of the premaxilla volume enhancement showed a final mean skeletal projection improvement of +9.4 mm, while the nasolabial angle decreased an average of + 0.6 mm. On the other hand, only small changes in nasal width were detected. Patient satisfaction with the functional and aesthetic outcomes at the 1-year follow-up was excellent. The pillow graft is an easy-to-handle technique that can be included in the armamentarium for moderately incrementing the maxillary sagittal dimension and enhancing lip support in the context of zygoma implant rehabilitation.

Three-dimensional evaluation of postoperative stability: a comparative study between surgery-first and surgery-late protocols.

The main objective of this study was to compare the stability of the surgery-first and surgery-late approaches according to the standardized centre protocols, by three-dimensional evaluation after 1 year of follow-up. A retrospective study was designed that included a test group (surgery-first protocol) and a control group (surgery-late protocol), with a follow-up period of at least 1 year (average 14 months; range 12-24 months). Stability was evaluated using linear and angular measurements by superimposing cone beam computed tomography images obtained at specific points in time: preoperatively, 1 month after surgery, and at the end of the orthodontic treatment. A total of 56 patients with a mean age of 32.2 ± 11.1 years were included in the study. After surgery there were significant changes in all of the measurements in at least one dimension in both groups (except for the transverse maxillary dimension), which remained stable at the end of the treatment, with no statistically significant differences between the two groups. At the 1-year follow-up, both groups presented a SNA angle relapse; this relapse was more significant in the surgery-late group (P = 0.031) and was present only in Class III patients (P = 0.013). In conclusion, an equivalent three-dimensional stability between surgery-first and surgery-late protocols was demonstrated after 1 year of follow-up when eligibility criteria were strictly adhered to.

Transmucosal pterygomaxillary disjunction using a piezoelectric device, in the context of the minimally invasive Le Fort I osteotomy protocol.

The aim of this study was to assess the accuracy and clinical implications of pterygomaxillary junction (PMJ) disjunction with a transmucosal PMJ osteotomy using a piezoelectric hand-piece device, in the context of Le Fort I osteotomy, by evaluating the level of PMJ disarticulation and the need for bone trimming around the pedicle. An ambidirectional 1-month follow-up cohort study was designed involving consecutive patients undergoing minimally invasive maxillary Le Fort I osteotomy through the twist technique. Two cohorts were defined according to whether or not the transmucosal PMJ osteotomy was performed. The site of PMJ disjunction was analysed radiographically. A total of 114 patients were included in the study, 57 in each group. The overall accuracy of the PMJ disjunction path was higher in the test group (43.9%) than in the control group (15.8%). Multiple logistic regression analysis identified the need for bone trimming (odds ratio 0.02; P < 0.001) and removal of the upper third molar (odds ratio 0.17; P < 0.001) as relevant factors. In conclusion, compared with the originally described twist technique, combination of the latter with the PMJ osteotomy increased its accuracy at the level of the PMJ. As a result, there is a decrease in resistance during down-fracture and decrease in the need for bone trimming around the pedicle, with preservation of the minimally invasive concept.

First national survey of residues of active substances in honeybee apiaries across Spain between 2012 and 2016.

This nationwide monitoring aimed to investigate the prevalence of residues of plant protection products (PPPs) and veterinary medicine products (VMPs) based on random selection of apiaries of Apis mellifera. For a three-year period (2012, 2013 and 2016), this study targeted 306 PPPs, VMPs and other active substances in 442 samples of bee bread honeycomb (BBHC) and 89 samples of honeybees collected from up to 177 apiaries. The results indicate that honeybees were most often exposed to residues of coumaphos, tau-fluvalinate, chlorfenvinphos, and acrinathrin, with a prevalence from a maximum of 98.8% to 49.4% in BBHC samples. Residues of coumaphos, tau-fluvalinate, amitraz (DMF + DMPF), carbendazim and orthophenylphenol were also frequently detected, from a maximum of 55.1% to 13.5% of the honeybee samples. Neonicotinoid residues, namely clothianidin and thiamethoxam, whose outdoor uses in crops are completely banned in EU, were not detected. Imidacloprid was found in 3.4% to 13.3% of samples during 2013 and 2016, respectively. Imidacloprid exceeded its acute toxicity (LD50) value for honey bees in two samples of BBHC. Fipronil was detected in 0.5% of the samples during 2013. The diversity of active substances found (% of different residues analyzed) ranged from 33.9% to 37.2% in BBHC from 2012, 2013 to 2016, and was of 26.5% in honeybees in 2016. In at least 54% of the samples, the total residue load was in the range of 200 to 1500 µg·kg-1. Up to 50% of BBHC samples were positive for one or two residues. No toxic residues for honeybees were detected in up to 88.8% of bee samples. This systematic surveillance of active substances assisted the evaluation of which target pesticides to look for and provided support to the competent authorities in the bee health decision-making.

Relevance of CT for the detection of septic foci: diagnostic performance in a retrospective cohort of medical intensive care patients.

AIM: To analyse the diagnostic yield of computed tomography (CT) in septic patients from a medical intensive care unit (ICU). MATERIALS AND METHODS: A full-text search of the department's radiological information system (RIS) retrieved 227 body CT examinations undertaken to search for a septic focus in 2018 from medical ICU patients. CT reports were categorised according to the identified foci. Clinical and laboratory information was gathered. Data were analysed statistically using descriptive statistics, diagnostic test quality criteria, binomial tests and chi-square test. RESULTS: A total of 227 CT examinations from 165 septic patients detected 264 foci, which were distributed as follows: 58.3% (n=154/264) chest, 26.5% (n=70/264) abdomen, 5.3% (n=14/264) genitourinary system, and 9.8% (n=26/264) other body regions. In 15.9% (n=36/227) no focus was identified on CT. Based on CT reports, 37.5% (n=99/264) of foci were graded as certain, 18.9% (n=50/264) as likely, and 15.9% (n=42/264) as possible infectious sources. Septic foci were detected using CT with 75.8% sensitivity (95% confidence interval [CI] 69.6-81.9%) and 59.46% specificity (95% CI 42.9-76.1%). The positive predictive value was 90.6% (95% CI 86-95.2%), with a negative predictive value of 32.4% (95% CI 21-43.8%). CONCLUSION: The present results confirm that body CT is a suitable rule-in test for septic patients in medical intensive care, although it cannot reliably rule out a septic focus. Follow-up CT examinations may reveal a septic source in the further course of a patient's hospital stay.

Effect of ultrasonication on waste activated sludge rheological properties and process economics.

The present study provides an overall view of the effect of the ultrasound treatment on waste activated sludge (WAS) rheological and dewatering properties as well as its impact on the economic balance of a theoretical wastewater treatment plant. The results showed that ultrasonication at 27,000 kJ/kg TS increased the soluble protein concentration (> 100%), bound water content (∼25%), and capillary suction time (> 100%) of WAS. The molecular weight distribution of the extracellular polymeric substances (EPS) revealed that the ultrasound treatment solubilised a portion of the peptides and low-molecular-weight proteins. The thixotropic behaviour of the WAS was analysed by means of a rheological structural model that defines the time evolution of a structural parameter as a function of kinetic coefficients for the breakdown and build-up processes. The ultrasound treatment reduced the kinetic coefficients for the breakdown process and changed the fast speed of alignment of flocs because of the reduction of WAS structures. Similarly, the creep tests revealed that the ultrasound treatment at 27,000 kJ/kg TS reduced the initial elasticity (∼80%) and the zero-shear rate viscosity (∼60%), which means that the internal structure of the WAS loosened and disrupted. Finally, a techno-economic analysis showed that ultrasonication was not yet economically favourable since its implementation increased 14% the net cost for WAS treatment and disposal. However, a sensitivity analysis illustrated that increasing electricity revenue and reducing biosolids disposal costs through improvement in WAS biodegradability is important to make ultrasound implementation economically attractive.

The Nereid on the rise: Platynereis as a model system.

The Nereid Platynereis dumerilii (Audouin and Milne Edwards (Annales des Sciences Naturelles 1:195-269, 1833) is a marine annelid that belongs to the Nereididae, a family of errant polychaete worms. The Nereid shows a pelago-benthic life cycle: as a general characteristic for the superphylum of Lophotrochozoa/Spiralia, it has spirally cleaving embryos developing into swimming trochophore larvae. The larvae then metamorphose into benthic worms living in self-spun tubes on macroalgae. Platynereis is used as a model for genetics, regeneration, reproduction biology, development, evolution, chronobiology, neurobiology, ecology, ecotoxicology, and most recently also for connectomics and single-cell genomics. Research on the Nereid started with studies on eye development and spiralian embryogenesis in the nineteenth and early twentieth centuries. Transitioning into the molecular era, Platynereis research focused on posterior growth and regeneration, neuroendocrinology, circadian and lunar cycles, fertilization, and oocyte maturation. Other work covered segmentation, photoreceptors and other sensory cells, nephridia, and population dynamics. Most recently, the unique advantages of the Nereid young worm for whole-body volume electron microscopy and single-cell sequencing became apparent, enabling the tracing of all neurons in its rope-ladder-like central nervous system, and the construction of multimodal cellular atlases. Here, we provide an overview of current topics and methodologies for P. dumerilii, with the aim of stimulating further interest into our unique model and expanding the active and vibrant Platynereis community.

Whole-body integration of gene expression and single-cell morphology.

Animal bodies are composed of cell types with unique expression programs that implement their distinct locations, shapes, structures, and functions. Based on these properties, cell types assemble into specific tissues and organs. To systematically explore the link between cell-type-specific gene expression and morphology, we registered an expression atlas to a whole-body electron microscopy volume of the nereid Platynereis dumerilii. Automated segmentation of cells and nuclei identifies major cell classes and establishes a link between gene activation, chromatin topography, and nuclear size. Clustering of segmented cells according to gene expression reveals spatially coherent tissues. In the brain, genetically defined groups of neurons match ganglionic nuclei with coherent projections. Besides interneurons, we uncover sensory-neurosecretory cells in the nereid mushroom bodies, which thus qualify as sensory organs. They furthermore resemble the vertebrate telencephalon by molecular anatomy. We provide an integrated browser as a Fiji plugin for remote exploration of all available multimodal datasets.

Ocean warming and freshening effects on lipid metabolism in coastal Antarctic phytoplankton assemblages dominated by sub-Antarctic species.

Marine phytoplankton can utilize different strategies to cope with ocean warming and freshening from glacial melting in polar regions, which are disproportionally impacted by global warming. In the present study, we investigated the individual and combined effects of a 4 °C increase in seawater temperature (T+) and a 4 psu decrease in salinity (S-) from ambient values on biomass, nutrient use, fatty acid composition and lipid damage biochemistry of natural phytoplankton assemblages from Potter Cove (25 de Mayo/King George Island, Antarctica). Experiments were conducted by exposing the assemblages to four treatments during a 7-day incubation period using microcosm located along shore from January 23 to 31, 2016. The N:P ratio decreased in all treatments from day 4 onwards, but especially under high temperature (T+). Lipid damage was mainly detected under S0T+ and S-T+ conditions, and it decreased when the production of the antioxidant α-tocopherol increased. This antioxidant protection resulted in a build-up of phytoplankton biomass, especially at T+. Under the combined effect of both stressors (S-T+), the concentration of ω3 fatty acids increased, potentially leading to higher-quality FA composition. These results, which were related to the dominance of sub-Antarctic species in phytoplankton assemblages, contribute to the understanding of the potential consequences of ocean warming and increase seawater freshening on the trophic webs of the Southern Ocean.

Determinantes sociales de salud y su relación con desnutrición infantil en dos comunidades étnicas colombianas / Social determinants and malnutrition of infants from two Colombian indigenous communities

RESUMEN Objetivo Identificar la relación entre los determinantes sociales de la salud y el estado nutricional de los niños pertenecientes a dos comunidades indígenas de la Costa Caribe colombiana, los wayús y los zenús. Métodos Estudio transversal que aborda el estado nutricional de los wayús y los zenús en edad preescolar y escolar. Incluyendo, además, estimación de determinantes sociales de la salud, referida por cuidadores de menores, que aborda algunos aspectos de ejes de desigualdad de tipo estructural (etnia, territorio, edad y sexo) e intermedios: vivienda, entorno y redes, mediante instrumentos de salud familiar. Se estandarizaron métricas de talla, peso e insumos de familiograma y ecomapa. Se estimaron medidas descriptivas de tipo frecuentistas y de medidas de tendencia central. Se estimó la odds ratio y su relación con variables independientes. Resultados La prevalencia de desnutrición global en la etnia Wayú fue del 59,1% IC (47,569,8) y en la etnia Zenú del 22,4% IC (15,9-30,4) para una p<0,001. Esta diferencia se relaciona con factores de riesgo intermediarios de saneamiento: inadecuadas excretas con OR=2.54; IC (1.42-4.53) p=0.002, la falta de empleo OR=1.94; IC (1.09-3.46) P=0.03 y falta de acceso a servicios de salud occidental OR=2.42; IC (1.34-4.40) p=0.005. Conclusiones La mejora en la nutrición requiere intervenir determinantes intermedios relacionados con saneamiento, modelo de salud, intersectorialidad de políticas en salud más enfoque étnico-cultural y territorial.

ABSTRACT Objetive To identify the relationship between the Health's Social Determinants and the nutritional status of children belonging to two indigenous communities on the Colombian Caribbean coast, the Wayú and the Zenú. Methods Cross-sectional study that addresses nutritional status in wayúes and zenúes at preschool and school age. Also including the estimation of health's social determinants, referred by minor's caregivers, which addresses some aspects of structural ¡nequality axes (ethnicity, territory, age, and sex) and intermediates: housing, environment, and networks, through health instruments family. Metrics for height, weight, and inputs for the familiogram and ecomap were standardized. Descriptive measures of frequencies and central tendency were estimated. Odds ratio and its relationship with ¡ndependent variables were estimated. Results The prevalence of global malnutrition in the Wayú ethnic group was 59.1% (47.5-69.8) and in the Zenú ethnic group 22.4% (15.9-30.4) for a p<0.001. This difference is related to intermedíate risk factors with sanitation: inadequate excreta with OR=2.54; CI (1.42-4.53) p=0.002, lack of employment OR=1.94; CI (1.09-3.46) P=0.03 and lack of access to western health services OR=2.42; IC (1.34-4.40) p=0.005. Conclusions Improvement in nutrition requires the intervention of intermediate determinants related to sanitation, health model, intersectoriality of health policies plus an ethnic-cultural and territorial focus.

A Systematic Review of Collective Evidences Investigating the Effect of Diabetes Monitoring Systems and Their Application in Health Care.

Introduction: Diabetes monitoring systems (DMS) are a possible approach for regular control of glucose levels in patients with Type 1 or 2 diabetes in order to improve therapeutic outcomes or to identify and modify inappropriate patient behaviors in a timely manner. Despite the significant number of studies observing the DMS, no collective evidence is available about the effect of all devices. Goal: To review and consolidate evidences from multiple systematic reviews on the diabetes monitoring systems and the outcomes achieved. Materials and methods: Internet-based search in PubMed, EMBASE, and Cochrane was performed to identify all studies relevant to the research question. The data regarding type of intervention, type of diabetes mellitus, type of study, change in clinical parameter(s), or another relevant outcome were extracted and summarized. Results: Thirty-three out of 1,495 initially identified studies, involving more than 44,100 patients with Type 1, Type 2, or gestational diabetes for real-time or retrospective Continuous Glucose Monitoring (CGMS), Sensor Augmented Pump Therapy (SAPT), Self-monitoring Blood Glucose (SMBG), Continuous subcutaneous insulin infusion (CSII), Flash Glucose Monitoring (FGM), Closed-loop systems and telemonitoring, were included. Most of the studies observed small nominal effectiveness of DMS. In total 11 systematic reviews and 15 meta-analyses, with most focusing on patients with Type 1 diabetes (10 and 6, respectively), reported a reduction in glycated hemoglobin (HbA1c) levels from 0.17 to 0.70% after use of DMS. Conclusion: Current systematic review of already published systematic reviews and meta-analyses suggests that no statistically significant difference exists between the values of HbA1c as a result of application of any type of DMS. The changes in HbA1c values, number and frequency of hypoglycemic episodes, and time in glucose range are the most valuable for assessing the appropriateness and effectiveness of DMS. Future more comprehensive studies assessing the effectiveness, cost-effectiveness, and comparative effectiveness of DMS are needed to stratify them for the most suitable diabetes patients' subgroups.

Variation between natural head orientation and Frankfort horizontal planes in orthognathic surgery patients: 187 consecutive cases.

The purpose of this study was to assess the relationship between the Frankfort horizontal (FH) and natural head orientation (NHO), their correlation between patients' malocclusion, and the impact of counterclockwise rotation (CCW) on the FH-NHO angle variation after orthognathic surgery. An evaluation of 187 consecutive patients was performed at the Maxillofacial Institute (Teknon Medical Center, Barcelona). FH-NHO° was measured pre- and postoperatively at 1 and 12 months, after three-dimensional (3D) superimposition using a software (Dolphin®). Patients were classified as follows: 3.2%, 48.7% and 48.1%, class I, II and III, respectively. Baseline FH-NHO° was significantly positive for patients with dentofacial deformities (2.73°±4.19 (2.12-3.33°, P<0.001). The impact of orthognathic surgery in FH-NHO° was greater in class II when compared with class III patients, with a variation of 2.04°±4.79 (P<0.001) and -1.20°±3.03 (P<0.001), respectively. FH-NHO° increased when CCW rotational movements were performed (P=0.006). The results of this study suggest that pre- and postoperative NHO differs from FH in orthognathic patients. The angle between FH and NHO is significantly larger in class III than in class II patients at baseline, which converges after orthognathic surgery when CCW rotation is performed. Therefore, NHO should be used as the real horizontal plane when planning for orthognathic surgery.

A three-year large scale study on the risk of honey bee colony exposure to blooming sunflowers grown from seeds treated with thiamethoxam and clothianidin neonicotinoids.

Despite the restriction of the use of neonicotinoids in the EU, including thiamethoxam and clothianidin, the debate over their risk on honey bees has not been fully settled. This study presents results of a three-year study working with 180 honey bee colonies in ten replicates. Colonies were sorted into three treatments (60 colonies per treatment) exposed to sunflower blooms grown from seeds treated with thiamethoxam, clothianidin and a non-treated control. Each colony was assessed at six moments: one before to exposition to sunflower, two during the exposition (short-time risk), two after exposition (medium-time risk) and one after wintering (long-time risk). The health and development of the colonies were assessed by monitoring adult bee population, brood development, status of the queen, food reserves and survival. No significant difference among treatments when raw data was considered. However, when evolution from initial status of the colony was evaluated, a significant difference was observed from the first week of exposure to sunflower blooms. In this period, the number of adult bees and the amount of brood were slightly lower in the bee hives exposed to neonicotinoids, although such differences disappeared in subsequent evaluations. The concentration of residues in samples of beebread and adult bees was at the level of ng·g-1. Magnitude of the effect of the treatment factor on the variability of colony health and development related parameters was low. The most important factor was the hive, followed by the replicate and year, and to a lesser extent the initial strength of the colonies.

Managing gestational diabetes mellitus using a smartphone application with artificial intelligence (SineDie) during the COVID-19 pandemic: Much more than just telemedicine.

We describe our experience in the remote management of women with gestational diabetes mellitus during the COVID-19 pandemic. We used a mobile phone application with artificial intelligence that automatically classifies and analyses the data (ketonuria, diet transgressions, and blood glucose values), making adjustment recommendations regarding the diet or insulin treatment.

Method to generate a large cohort in-silico for type 1 diabetes.

BACKGROUND AND OBJECTIVE: In the last decade, several technological solutions have been proposed as artificial pancreas systems able to treat type 1 diabetes; most often they are built based on a control algorithm that needs to be validated before it is used with real patients. Control algorithms are usually tested with simulation tools that integrate mathematical models related mainly to the glucose-insulin dynamics, but other variables can be considered as well. In general, the simulators have a limited set of subjects. The main goal of this paper is to propose a new computational method to increase the number of virtual subjects, with physiological characteristics, included in the original mathematical models. METHODS: A subject is defined by a set of parameters given by a mathematical model. From the available reduced number of subjects in the model, the covariance of each parameter of every subject is obtained to establish a mathematical relationship. Then, new sets of parameters are calculated using linear regression methods; this generates larger cohorts, which allows for testing insulin therapies in open-loop or closed-loop scenarios. The new method proposed here increases the number of subjects in a virtual cohort using two versions of Hovorka's mathematical model. RESULTS: Two covariant cohorts are obtained with linear regression. Both cohorts are clustered to avoid overlapping in the glucose-insulin dynamics and are compared in terms of their qualitative and quantitative behaviours in the normoglycemic range. As a result, there have been generated two larger cohorts (256 subjects) than the original population, which contributes to improving the variability in in-silico tests. In addition, for analysing the characteristics of the covariant generation method, two random cohorts have been generated, where the parameters are obtained individually and independently from each other, exhibiting only distribution limitations so that these cohorts do not have physiological subjects. CONCLUSIONS: The proposed methodology has enabled the generation of a large cohort of 256 subjects, with different characteristics that are plausible in the T1DM population, significantly increasing the number of available subjects in existing mathematical models. The proposed methodology does not limit the number of subjects that can be generated and thus, it can be used to increase the number of cohorts provided by other mathematical models in diabetes, or even other scientific problems.

Quantitative determination of pesticide residues in specific parts of bee specimens by nanoflow liquid chromatography high resolution mass spectrometry.

The presence of pesticide residues in bees is of great interest, given the central role of bees as indicators for environmental assessment. The goal of this article is to propose a method to capture enhanced chemical information for these central environmental indicators. Most of the methods rely on the analysis of pooled samples rather than individual specimens due to practical sample preparation method considerations and limitations in sensitivity. This leads to miss information on the mapping of pesticides and actual amount of pesticide per specimen. In this article, a nanoflow liquid chromatography system coupled to high resolution mass spectrometry (using a hybrid quadrupole-Orbitrap instrument) has been applied for the development of a multiresidue pesticide method for the determination of 162 multiclass pesticides in specific part of honeybee samples (ca. abdomen, head or thorax). The reduced flow rate provided an enhancement in sensitivity and a strong reduction of matrix effects, thus only a quick and simple ultrasound assisted extraction using minute amount of sample was required. Satisfactory results were obtained for all tested analytes with concentration levels detected lower than 0.5 ng g-1 in all cases, thus being acceptable for monitoring purposes. Matrix effect was negligible for 94% of compounds. Extraction recoveries ranged from 70% to 105%, being within SANTE guidelines. Finally, the applicability of the method was demonstrated, by successful application to the analysis of contaminated honeybee samples, extracting useful information from specific bee parts of single specimens, thus, enabling pseudo spatially resolved chemical information.

Automated Insulin Delivery: The Artificial Pancreas Technical Challenges.

BACKGROUND: The automation of glucose control has been an important goal of diabetes treatment for many decades. The first artificial pancreas experiences were in-hospital, closely supervised, small-scale, and short-term studies that demonstrated their superiority over continuous subcutaneous insulin infusion therapy. At present, long-term outpatient studies are being conducted in free-living scenarios. AREAS OF UNCERTAINTY: The integration of multiple devices increases patients' burden and the probability of technical risks. Control algorithms must be robust to manage disturbance variables, such as physical exercise, meal composition, stress, illness, and circadian variations in insulin sensitivity. Extra layers of safety could be achieved through remote supervision. Dual-hormone systems reduce the incidence and duration of hypoglycemia, but the availability of stable pumpable glucagon needs to be solved. Faster insulin analogues are expected to improve all types of artificial pancreas. THERAPEUTIC ADVANCES: Artificial pancreas safety and feasibility are being demonstrated in outpatient studies. Artificial pancreas use increases the time of sensor-measured glucose in near-normoglycemia and reduces the risk of hyperglycemia and hypoglycemia. The benefits are observed both in single- and dual-hormone algorithms and in full- or semi-closed loop control. A recent meta-analysis including 41 randomized controlled trials showed that artificial pancreas use achieves a reduction of time in hyperglycemia (2 hours less than control treatment) and in hypoglycemia (20 minutes less); mean levels of continuous glucose sensor fell by 8.6 mg/dL over 24 hours and by 14.6 mg/dL overnight. The OpenAPS community uses Do It Yourself artificial pancreas in the real world since 2013, and a recent retrospective cross-over study (n = 20) compared continuous glucose sensor readings before and after initiation: mean levels of blood glucose fell by 7.4 mg/dL over 24 hours and time in range increased from 75.8% to 82.2% (92 minutes more). CONCLUSIONS: The outpatient use of artificial pancreas is safe and improves glucose control in outpatients with type 1 diabetes compared with the use of any type of insulin-based treatment. The availability of open-source solutions and data sharing is needed to foster the development of new artificial pancreas approaches and to promote the wide use of Big Data tools for knowledge discovery, decision support, and personalization.